The Onderstepoort journal of veterinary research最新文献

{"title":"Sero-epidemiological survey and risk factors associated with bovine brucellosis among slaughtered cattle in Nigeria.","authors":"Victor O Akinseye,&nbsp;Hezekiah K Adesokan,&nbsp;Akwoba J Ogugua,&nbsp;Folashade J Adedoyin,&nbsp;Patricia I Otu,&nbsp;Ayi V Kwaghe,&nbsp;Noah O Kolawole,&nbsp;Oyinye J Okoro,&nbsp;Charity A Agada,&nbsp;Adeniyi O Tade,&nbsp;Olufemi O Faleke,&nbsp;Anyanwu L Okeke,&nbsp;Ibikunle M Akanbi,&nbsp;Mofoluwake M Ibitoye,&nbsp;Morenike O Dipeolu,&nbsp;Emma J Dale,&nbsp;Perrett Lorraine,&nbsp;Andrew V Taylor,&nbsp;Emmanuel A Awosanya,&nbsp;Eniola O Cadmus,&nbsp;Judy A Stack,&nbsp;Simeon I Cadmus","doi":"10.4102/ojvr.v83i1.1002","DOIUrl":"https://doi.org/10.4102/ojvr.v83i1.1002","url":null,"abstract":"<p><p>Bovine brucellosis is endemic in Nigeria; however, limited data exist on nationwide studies and risk factors associated with the disease. Using a cross-sectional sero-epidemiological survey, we determined the prevalence of and risk factors for brucellosis in slaughtered cattle in three geographical regions of Nigeria. Serum samples from randomly selected unvaccinated cattle slaughtered over a period of 3 years (between December 2010 and September 2013) from northern, southern and south-western Nigeria were tested for antibodies to Brucella abortus using the Rose Bengal test. Data associated with risk factors of brucellosis were analysed by Stata Version 12. In all, 8105 cattle were screened. An overall seroprevalence of 3.9% (315/8105) was recorded by the Rose Bengal test, with 3.8%, 3.4% and 4.0% from the northern, southern and south-western regions, respectively. Bivariate analysis showed that cattle screened in northern Nigeria were less likely to be seropositive for antibodies to Brucella spp. than those from south-western Nigeria (odds ratio = 0.94; 95% confidence interval: 0.73-1.22). However, logistic regression analysis revealed that breed ( p = 0.04) and sex ( p £ 0.0001) of cattle were statistically significant for seropositivity to Brucella spp. The study found that brucellosis was endemic at a low prevalence among slaughtered cattle in Nigeria, with sex and breed of cattle being significant risk factors. Considering the public health implications of brucellosis, we advocate coordinated surveillance for the disease among diverse cattle populations in Nigeria, as is carried out in most developed countries. </p>","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":" ","pages":"a1002"},"PeriodicalIF":2.7,"publicationDate":"2016-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/ojvr.v83i1.1002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34534822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis and genotyping of African swine fever viruses from 2015 outbreaks in Zambia.","authors":"Jonas Thoromo,&nbsp;Edgar Simulundu,&nbsp;Herman M Chambaro,&nbsp;Liywalii Mataa,&nbsp;Caesar H Lubaba,&nbsp;Girja S Pandey,&nbsp;Ayato Takada,&nbsp;Gerald Misinzo,&nbsp;Aaron S Mweene","doi":"10.4102/ojvr.v83i1.1095","DOIUrl":"https://doi.org/10.4102/ojvr.v83i1.1095","url":null,"abstract":"<p><p>In early 2015, a highly fatal haemorrhagic disease of domestic pigs resembling African swine fever (ASF) occurred in North Western, Copperbelt, and Lusaka provinces of Zambia. Molecular diagnosis by polymerase chain reaction targeting specific amplification of p72 (B646L) gene of ASF virus (ASFV) was conducted. Fourteen out of 16 domestic pigs from the affected provinces were found to be positive for ASFV. Phylogenetic analyses based on part of the p72 and the complete p54 (E183L) genes revealed that all the ASFVs detected belonged to genotypes I and Id, respectively. Additionally, epidemiological data suggest that the same ASFV spread from Lusaka to other provinces possibly through uncontrolled and/or illegal pig movements. Although the origin of the ASFV that caused outbreaks in domestic pigs in Zambia could not be ascertained, it appears likely that the virus may have emerged from within the country or region, probably from a sylvatic cycle. It is recommended that surveillance of ASF, strict biosecurity, and quarantine measures be imposed in order to prevent further spread and emergence of new ASF outbreaks in Zambia.</p>","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":" ","pages":"a1095"},"PeriodicalIF":2.7,"publicationDate":"2016-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/ojvr.v83i1.1095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34534336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medicinal plants used to control internal and external parasites in goats.","authors":"Marcia Sanhokwe,&nbsp;Johnfisher Mupangwa,&nbsp;Patrick J Masika,&nbsp;Viola Maphosa,&nbsp;Voster Muchenje","doi":"10.4102/ojvr.v83i1.1016","DOIUrl":"https://doi.org/10.4102/ojvr.v83i1.1016","url":null,"abstract":"<p><p>The use of medicinal plants plays a major role in the primary health care of animals in South Africa. A survey was conducted to document medicinal plants used to control parasites in goats in Kwezi and Ntambethemba villages in the Eastern Cape Province, South Africa. Information from 50 farmers and 3 herbalists was obtained through the use of a structured questionnaire, and a snowball sampling technique was used to identify key informants. The obtained data were analysed using PROC FREQ of SAS (2003), and fidelity level values were determined to estimate the healing potential of the mentioned plants. The survey revealed nine plant species belonging to eight families that were used to control parasites in goats. Asphodelaceae (22.22%) was the most frequently used plant family. Leaves were the most used plant parts, constituting 60.38%. They were prepared either as infusions or decoctions of single plants or in mixtures. Aloe ferox, Acokanthera oppositifolia and Elephantorrhiza elephantina were the plants having the highest fidelity level for their use to control parasites, each scoring 100%, followed by Albuca setosa (83.33%). The study revealed low knowledge about ethnoveterinary medicine in the study area. It also revealed that information on ethno-veterinary medicine in this area is mostly confined to older people and there is danger that this knowledge can be lost before being passed on to other generations. Therefore, there is an urgent need to document information on these plant species so that the future generation can benefit. Further investigation should be carried out to validate the efficacy and safety of the above-mentioned plants so as to provide cheap alternative ways of controlling parasites. </p>","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":" ","pages":"a1016"},"PeriodicalIF":2.7,"publicationDate":"2016-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/ojvr.v83i1.1016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34534335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serosurvey of peste des petits ruminants virus in small ruminants from different agro-ecological zones of Nigeria","authors":"T. Woma, P. Ekong, Dauda Garba Bwala, J. Ibu, L. Ta'ama, D. Dyek, Ladi Saleh, D. Shamaki, D. Kalla, D. Bailey, H. Kazeem, M. Quan","doi":"10.4102/ojvr.v83i1.1035","DOIUrl":"https://doi.org/10.4102/ojvr.v83i1.1035","url":null,"abstract":"Peste des petits ruminants, caused by the peste des petits ruminants virus (PPRV), is a highly contagious and economically important transboundary viral disease of domestic and wild small ruminants and a major hindrance to small-ruminant production in Nigeria. The seroprevalence and distribution of PPRV antibodies in small ruminants in rural households, farms, live animal markets and slaughter slabs across the six different agro-ecological zones of Nigeria were determined. A total of 4548 serum samples from 3489 goats and 1059 sheep were collected in 12 states. A PPRV competitive enzyme-linked immunosorbent assay was used to test the samples and the data analysed with R statistical software version 3.0.1. The study animals included all ages and both sexes. The overall prevalence estimate of sera positive for PPRV antibodies was 23.16% (n = 1018 positive samples per 4548 total samples, 95% confidence interval: 21.79% – 24.57%). There were significant differences in the seroprevalence between the states (p = 0.001). Taraba State had the highest seroprevalence of 29.51%, whilst the lowest seroprevalence of 14.52% was observed in Cross River State. There were no significant differences in the PPRV seroprevalence between male and female animals (p = 0.571), age (p = 0.323) and between species (p = 0.639). These data indicate the current seroprevalence to PPRV in the small-ruminant population in Nigeria.","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131922189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-series analysis of ruminant foetal wastage at a slaughterhouse in North Central Nigeria between 2001 and 2012","authors":"N. Alhaji, Ismail Ayoade Odetokun, A. Shittu, J. Onyango, U. Chafe, M. S. Abubakar, I. Muraina, F. Fasina, Hu Suk Lee","doi":"10.4102/ojvr.v82i1.1010","DOIUrl":"https://doi.org/10.4102/ojvr.v82i1.1010","url":null,"abstract":"In developing countries, foetal wastage from slaughtered ruminants and the associated economic losses appear to be substantial. However, only a limited number of studies have comprehensively evaluated these trends. In the current study, secondary (retrospective) and primary data were collected and evaluated to estimate the prevalence of foetal wastage from cattle, sheep and goats slaughtered at an abattoir in Minna, Nigeria, over a 12-year period (January 2001 – December 2012). Time-series modelling revealed substantial differences in the rate of foetal wastage amongst the slaughtered species, with more lambs having been wasted than calves or kids. Seasonal effects seem to influence rates of foetal wastage and certain months in the year appear to be associated with higher odds of foetal wastage. Improved management systems are suggested to reduce the risk of foetal losses.","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122379605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatio-temporal patterns and movement analysis of pigs from smallholder farms and implications for African swine fever spread, Limpopo province, South Africa","authors":"F. Fasina, J. M. Mokoele, B. T. Spencer, L. van Leengoed, Yvette Bevis, I. Booysen","doi":"10.4102/ojvr.v82i1.795","DOIUrl":"https://doi.org/10.4102/ojvr.v82i1.795","url":null,"abstract":"Infectious and zoonotic disease outbreaks have been linked to increasing volumes of legal and illegal trade. Spatio-temporal and trade network analyses have been used to evaluate the risks associated with these challenges elsewhere, but few details are available for the pig sector in South Africa. Regarding pig diseases, Limpopo province is important as the greater part of the province falls within the African swine fever control area. Emerging small-scale pig farmers in Limpopo perceived pig production as an important means of improving their livelihood and an alternative investment. They engage in trading and marketing their products with a potential risk to animal health, because the preferred markets often facilitate potential long-distance spread and disease dispersal over broad geographic areas. In this study, we explored the interconnectedness of smallholder pig farmers in Limpopo, determined the weaknesses and critical control points, and projected interventions that policy makers can implement to reduce the risks to pig health. The geo-coordinates of surveyed farms were used to draw maps, links and networks. Predictive risks to pigs were determined through the analyses of trade networks, and the relationship to previous outbreaks of African swine fever was postulated. Auction points were identified as high-risk areas for the spread of animal diseases. Veterinary authorities should prioritise focused surveillance and diagnostic efforts in Limpopo. Early disease detection and prompt eradication should be targeted and messages promoting enhanced biosecurity to smallholder farmers are advocated. The system may also benefit from the restructuring of marketing and auction networks. Since geographic factors and networks can rapidly facilitate pig disease dispersal over large areas, a multi-disciplinary approach to understanding the complexities that exist around the animal disease epidemiology becomes mandatory.","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134530979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of oxytetracycline residues in cattle meat marketed in the Kilosa district, Tanzania","authors":"Zuhura I. Kimera, R. Mdegela, C. N. Mhaiki, E. Karimuribo, F. Mabiki, H. Nonga, J. Mwesongo","doi":"10.4102/ojvr.v82i1.911","DOIUrl":"https://doi.org/10.4102/ojvr.v82i1.911","url":null,"abstract":"Oxytetracycline is used to treat various diseases in cattle. However, its use may be associated with unacceptable residue levels in food. Oxytetracycline residues in tissues from indigenous cattle were determined in a cross-sectional study conducted in the Kilosa district, Tanzania, between November 2012 and April 2013. A total of 60 tissue samples, including muscle, liver and kidney, were collected from slaughterhouses and butchers and analysed for oxytetracycline using high-performance liquid chromatography. Oxytetracycline residues were found in 71.1% of the samples, of which 68.3% were above acceptable regulatory levels. The mean concentration of oxytetracycline across tissues was 3401.1 µg/kg ± 879.3 µg/kg; concentrations in muscle, liver and kidney were 2604.1 µg/kg ± 703.7 µg/kg, 3434.4 µg/kg ± 606.4 µg/kg and 3533.1 µg/kg ± 803.6 µg/kg, respectively. High levels of oxytetracycline residue in meat from indigenous cattle may pose a health threat to consumers in Kilosa. The findings possibly reflect a general lack of implementation of recommended withdrawal periods, ignorance about drug use and lack of extension services. Strict regulation of the use of antimicrobial drugs in the livestock industry and associated testing of animal-derived food sources prior to marketing are required.","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116065356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of multidrug-resistant fatal colisepticaemia in weanling pigs","authors":"F. Fasina, Dauda Garba Bwala, E. Madoroba","doi":"10.4102/ojvr.v82i1.986","DOIUrl":"https://doi.org/10.4102/ojvr.v82i1.986","url":null,"abstract":"Abstract Escherichia coli is usually a benign commensal of the gut microflora. However, when E. coli acquires virulence genes it can multiply rapidly and cause disease through colonisation of the intestinal mucosa. Escherichia coli can become a significant pathogen in young pigs. We report an investigation of fatal colisepticaemia in weanling pigs from emerging farms where piglets and weaners were diarrhoeic and the mortality rate ranged between 15% and 70% in each litter. Faecal and tissue samples were processed for histopathology, bacteriology and molecular biology (multiplex and monoplex polymerase chain reaction) and we recovered enteroaggregative multidrug-resistant E. coli producing EAST-1 enterotoxin. An association between poor housing conditions and the observed cases was established and future management programmes were recommended to reduce the impact of such pathogens. Enteroaggregative E. coli is becoming a major problem in the pig industry. It therefore becomes necessary to establish the full impact of E. coli on the South African pig industry and to determine the geographic extent of the problem.","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132408305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Equine encephalosis in Thoroughbred foals on a South African stud farm","authors":"J. Grewar, P. Thompson, Carina W. Lourens, A. Guthrie","doi":"10.4102/ojvr.v82i1.966","DOIUrl":"https://doi.org/10.4102/ojvr.v82i1.966","url":null,"abstract":"Thoroughbred foal body temperature data were collected from shortly after birth until shortly after weaning during the 2007/2008 season on a stud farm in the Western Cape Province of South Africa. Equine encephalosis (EE) caused by EE virus (EEV) serotype 4 (EEV-4) occurred in the foal group during the first autumn after their birth (March and April 2008). A descriptive study was undertaken to provide data on the EEV maternal antibody status, the association between pyrexia and EEV infection, and the incidence of infection amongst the foals prior to and during the episode. This included the frequent capturing of foal body temperature data and regular collection of serum and whole blood during pyretic episodes. Infection by EEV was determined using both virological and serological methods. A high EE incidence of at least 94% occurred amongst the foal cohort, despite the fact that 37% of foals had previously shown maternal antibody to EEV-4. Pyrexia in foals was not directly associated with EE infection and 41% of infected foals showed no detectable pyretic episode. Information obtained from this EE episode showed the high incidence of EEV infection in foals during the first autumn after their birth. Monitoring foal body temperature can alert farmers to outbreaks of infectious disease, such as EE. These results are relevant to the epidemiology of EE and facilitate greater understanding of it as a differential diagnosis of African horse sickness (AHS), given that EE and AHS have similar epidemiologic profiles.","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116287306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular assessment of the African swine fever virus status of Ornithodoros ticks from Swaziland.","authors":"Carin I Boshoff,&nbsp;Armanda D S Bastos,&nbsp;Mzwandi M Dube,&nbsp;Livio Heath","doi":"10.4102/ojvr.v81i1.846","DOIUrl":"https://doi.org/10.4102/ojvr.v81i1.846","url":null,"abstract":"<p><p>African swine fever (ASF) is an economically significant haemorrhagic disease of domestic pigs. It is caused by the African swine fever virus (ASFV), a deoxyribonucleic acid (DNA)arbovirus. Argasid ticks of the genus Ornithodoros, which are widely distributed throughout southern Africa, play a primary role in virus maintenance and spread within the endemic sylvatic cycle. The ASF status of Swaziland is unknown, but this land-locked country is surrounded by ASF-positive countries, has a burgeoning pig industry and sylvatic cycle hosts present within its borders. In this first assessment of ASF status, warthog burrows in seven nature reserves and game management areas in Swaziland were investigated for tick and virus presence. Tick infestation rates of between 33.3% - 88.8% were recovered for the four Ornithodoros-infested reserves. A total of 562 ticks were screened for virus genome presence using a duplex Polymerase Chain Reaction (PCR) that targets the C-terminal end of the p72 gene of the ASFV and confirms DNA integrity through amplification of the 16S rRNA tick host gene. All samples were negative for virus genome presence and positive for the tick genome target. Nucleotide sequencing of the latter confirmed that Ornithodoros ticks from Swaziland are identical to those from the Kruger National Park in South Africa across the gene region characterised. Whilst this first evaluation of ASF presence in Swaziland indicates that the virus does not appear to be present in the key virus vector, the presence of sylvatic cycle hosts, together with the country's proximity to ASF-affected countries calls for expanded investigations and regular monitoring of the ASF status of Swaziland. </p>","PeriodicalId":107337,"journal":{"name":"The Onderstepoort journal of veterinary research","volume":" ","pages":"E1-5"},"PeriodicalIF":2.7,"publicationDate":"2014-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/ojvr.v81i1.846","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33946320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}