Clinical chemistry最新文献_第5页

HOPEing That a PEACEful Resolution for the Clinical Utility of High-Sensitivity Cardiac Troponin in the Ambulatory Setting Will Improve Laboratory Testing. 希望高敏感性心肌肌钙蛋白在门诊环境中的临床应用的和平解决将改善实验室检测。

IF 7.1 2区医学

Clinical chemistry Pub Date : 2025-03-27 DOI: 10.1093/clinchem/hvaf034

Peter A Kavsak

引用次数: 0

Vitamin B12 Deficiency and New Recommendations by the National Institute for Health and Care Excellence: A Challenging Clinical and Laboratory Topic. 维生素B12缺乏症和国家健康和护理卓越研究所的新建议：一个具有挑战性的临床和实验室主题。

IF 7.1 2区医学

Clinical chemistry Pub Date : 2025-03-27 DOI: 10.1093/clinchem/hvaf037

Simona Ferraro, Simona Da Molin, Cristina Cereda, Gianvincenzo Zuccotti, Santica Marcovina, Bruno Mario Cesana

引用次数: 0

Incidental Findings of Maternal Cancer in Noninvasive Prenatal Testing through Cell-Free DNA Sequencing. 通过无细胞DNA测序在无创产前检测中偶然发现的母亲癌症。

IF 7.1 2区医学

Clinical chemistry Pub Date : 2025-03-21 DOI: 10.1093/clinchem/hvaf014

Daniel J Smit, Klaus Pantel

引用次数: 0

Pediatric Vaccine-Induced Antibody Thresholds: Rethinking Pre-Immunosuppression Serologic Testing and Revaccination Implications 儿童疫苗诱导的抗体阈值：重新思考免疫抑制前血清学检测和重新接种的意义

IF 9.3 2区医学

Clinical chemistry Pub Date : 2025-03-19 DOI: 10.1093/clinchem/hvaf020

Megan Culler Freeman, Adam Sinder, Grace Conway, Sarah Chamseddine, Mariam Faiz Nassar, Bradley J Wheeler, Adam Anderson, Sarah E Wheeler

{"title":"Pediatric Vaccine-Induced Antibody Thresholds: Rethinking Pre-Immunosuppression Serologic Testing and Revaccination Implications","authors":"Megan Culler Freeman, Adam Sinder, Grace Conway, Sarah Chamseddine, Mariam Faiz Nassar, Bradley J Wheeler, Adam Anderson, Sarah E Wheeler","doi":"10.1093/clinchem/hvaf020","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf020","url":null,"abstract":"Background Immune response to vaccination is assessed when adequate vaccine protection is in question or immunosuppression is imminent through measurement of antibody levels, which wane as time from vaccination increases. The serologic cutoff value for adequate response is based on thresholds derived from studies in adults, and age-appropriate thresholds for children have not been established. We sought to investigate age-specific differences in antibody levels in healthy children to guide determination of vaccine immunity status when clinically indicated. Methods This cross-sectional study assessed clinical serology for measles, mumps, rubella (MMR), varicella, and hepatitis B (HepB) in an age-stratified cohort of 471 healthy children who were up to date for vaccination (1 to 18 years). Remnant specimens with sufficient volume were collected from July 23, 2019, to November 17, 2020, as convenience samples and chart reviewed for inclusion. Results While children of all ages had detectable titers to MMR, median titers for HepB and varicella waned by ages 11 to 12 and 9 to 10 years, respectively. Children had titers above adult thresholds for MMR at all measured timepoints, retrospectively resulting in 24.6% (95% CI, 21.6%–27.8%) of children having an inappropriate MMR classification when adult instead of pediatric thresholds were used. Current use of HepB and varicella serology may be inappropriate due to the rapid waning of titers. The adequacy of an individual’s response to one vaccine component did not infer adequate responses to other components. Conclusions Application of age-appropriate reference intervals for vaccine serologic tests will provide a foundation for improved treatment recommendations and standards of care.","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"90 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oropouche Virus: The Next (Re)Emerging Arboviral Threat? Oropouche病毒：下一个（重新）出现的虫媒病毒威胁？

IF 7.1 2区医学

Clinical chemistry Pub Date : 2025-03-12 DOI: 10.1093/clinchem/hvaf017

Portia Mira, Elitza S Theel

引用次数: 0

Enrichment of Microbial DNA in Plasma to Improve Pathogen Detection in Sepsis: A Pilot Study 血浆中微生物DNA的富集提高败血症病原体检测的初步研究

IF 9.3 2区医学

Clinical chemistry Pub Date : 2025-03-11 DOI: 10.1093/clinchem/hvaf011

Eddie G Dominguez, Bradon R McDonald, Haikun Zhang, Michelle D Stephens, Elise C Dietmann, Megan Nedden, Nicole Byington, Sydney Thompson, Mary Junak, Caitlin S Pepperell, Mehreen T Kisat

{"title":"Enrichment of Microbial DNA in Plasma to Improve Pathogen Detection in Sepsis: A Pilot Study","authors":"Eddie G Dominguez, Bradon R McDonald, Haikun Zhang, Michelle D Stephens, Elise C Dietmann, Megan Nedden, Nicole Byington, Sydney Thompson, Mary Junak, Caitlin S Pepperell, Mehreen T Kisat","doi":"10.1093/clinchem/hvaf011","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf011","url":null,"abstract":"Background Diagnosis of sepsis and timely identification of pathogens in critically ill patients remains challenging. Plasma metagenomic sequencing to detect microbial cell-free DNA (mDNA) has shown promise, but low abundance of mDNA in plasma limits sensitivity and necessitates high sequencing depth. mDNA is shorter and more fragmented than human cell-free DNA. Here, we evaluated whether combining single-stranded DNA (ssDNA) sequencing library preparation and size selection can enrich mDNA and improve pathogen detection. Methods We prospectively enrolled 48 trauma patients and collected daily blood samples during the first 10 days of intensive care unit (ICU) admission. For patients with culture-proven infections, we extracted plasma DNA, prepared double-stranded DNA (dsDNA) and ssDNA sequencing libraries, and applied size selection to exclude fragments &gt;110 bp. Following sequencing, we performed taxonomic classification, and evaluated differences in mDNA fractions and in sensitivity for pathogen detection (compared to background noise). Results We analyzed 46 plasma samples from 5 patients who developed culture-proven infections, including 17 samples coincident with positive microbial cultures. Size-selected ssDNA libraries showed the total mDNA fraction 204-fold higher on average than conventional dsDNA libraries (P &lt; 0.0001). However, for pathogen-specific DNA (at the genus level), the highest sensitivity was observed in size-selected dsDNA (82%), compared to dsDNA (41%), ssDNA (71%), and size-selected ssDNA (35%) library preparations. Conclusions Our results demonstrate that combining ssDNA library preparation together with fragment size selection improves mDNA yield, potentially reducing sequencing requirements. However, at the genus level, this combination also increases background noise, which limits sensitivity for pathogen detection.","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"213 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Annotation of Complete ABO Alleles and Resolution of ABO Variants by an Improved Full-Length ABO Haplotype Sequencing 基于改进的ABO全长单倍型测序的ABO全等位基因综合注释和ABO变异解析

IF 9.3 2区医学

Clinical chemistry Pub Date : 2025-03-06 DOI: 10.1093/clinchem/hvaf015

Yanling Ying, Jingjing Zhang, Xiaozhen Hong, Wenjing Yuan, Kairong Ma, Xinyu Huang, Xianguo Xu, Faming Zhu

{"title":"Comprehensive Annotation of Complete ABO Alleles and Resolution of ABO Variants by an Improved Full-Length ABO Haplotype Sequencing","authors":"Yanling Ying, Jingjing Zhang, Xiaozhen Hong, Wenjing Yuan, Kairong Ma, Xinyu Huang, Xianguo Xu, Faming Zhu","doi":"10.1093/clinchem/hvaf015","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf015","url":null,"abstract":"Background Full-length ABO haplotype sequencing is crucial for accurate genotyping, reference gene annotation, and molecular mechanism analysis of its variants. However, there is currently a deficiency of comprehensive annotation for full-length ABO haplotypes, spanning from the 5′ untranslated region (UTR) to the 3′ UTR. Methods Two sets of specimens (79 random blood donors and 47 ABO variants) were tested. The full-length ABO gene spanning the 5′ UTR to the 3′ UTR was amplified using an improved one-step ultra-long-range PCR with a pair of PCR suppression primers. A single-molecule real-time library was constructed, and ABO haplotype sequencing was performed. Data analysis including basecalling, aligning, variant calling, clustering, and variant annotation were performed. Results The amplicon measured 26.1 kb without splicing, representing the most complete ABO gene reported to date. The complete ABO haplotype sequence was obtained via long-read sequencing. The comprehensive ABO reference alleles were obtained and the ABO sequence patterns within each allele in a Chinese population were further classified. The full-length ABO gene haplotype analysis technique effectively resolved ABO variants with structural variations (SVs), including large fragment deletions, inversions, recombination, and chimeras. Conclusions Full-length ABO haplotype sequencing filled a gap that was missing with respect to the 3′ UTR sequences of ABO alleles and can advance blood group genomic analysis, aiding in ABO gene function analysis, evolutionary studies, and the resolution of ABO variants.","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"18 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary on A Pulmonary Nodule with an Unexpected Mutation Profile. 一种具有意外突变特征的肺结节的评论。

IF 7.1 2区医学

Clinical chemistry Pub Date : 2025-03-03 DOI: 10.1093/clinchem/hvae204

Viral Patel, Sheena Bhalla

引用次数: 0

Advances in Cardiac Troponin Composition Assays: A Step Closer to the Clinic? 心肌肌钙蛋白组成测定的进展：离临床又近了一步？

IF 7.1 2区医学

Clinical chemistry Pub Date : 2025-03-03 DOI: 10.1093/clinchem/hvae206

Xander M R van Wijk, Sander A J Damen

引用次数: 0

An AI Model (LORIS) to Predict Immune Checkpoint Blockade Response in Cancer: A Clinical Data Science Perspective. 预测癌症免疫检查点阻断反应的人工智能模型（LORIS）：临床数据科学视角。