Contemporary Clinical Dentistry最新文献_第7页

Comparative Evaluation of the Bonding Efficacy of Multimode Adhesive, Two-Step Self-Etch Adhesive, and a Total-Etch System to Pulpal Floor Dentin - An In vitro Study. 多模粘合剂、两步自蚀刻粘合剂和全蚀刻系统对牙髓基牙本质结合效果的比较评估——体外研究。

IF 1.2

Contemporary Clinical Dentistry Pub Date : 2023-04-01 Epub Date: 2023-02-07 DOI: 10.4103/ccd.ccd_754_21

Dhanya Valsan, Sajeev Bhaskaran, Joy Mathew, Krishnan Hari, Joseph Joy

{"title":"Comparative Evaluation of the Bonding Efficacy of Multimode Adhesive, Two-Step Self-Etch Adhesive, and a Total-Etch System to Pulpal Floor Dentin - An In vitro Study.","authors":"Dhanya Valsan, Sajeev Bhaskaran, Joy Mathew, Krishnan Hari, Joseph Joy","doi":"10.4103/ccd.ccd_754_21","DOIUrl":"10.4103/ccd.ccd_754_21","url":null,"abstract":"Background: Postendodontic restoration with composite resins has gained popularity due to the development of more reliable adhesives with simplified bonding procedures and less technique sensitivity. Pulpal floor dentin is a complex biological structure and a more challenging bonding substrate. Hence, appropriate selection of the bonding agent for these regions of the tooth is important.Aims: To compare the shear bond strength of a new generation of dental adhesives referred to as multimode adhesives, two-step self-etch adhesive, and a total-etch system to pulpal floor dentin.Materials and methods: Sixty human mandibular molars were sectioned with a slow speed diamond disc to expose the pulp chamber and divided into four groups of 15 teeth each, based on the bonding agent used. Group A: Single bond universal (self-etch); Group B: Single Bond Universal (etch-and-rinse), Group C: Clearfil SE Bond, Group D: Adper Single Bond 2. Using a split Teflon mold, composite cylinders were built on the prepared dentinal surfaces. The shear bond strength was then evaluated with the universal testing machine.Results: Two step self-etch adhesives recorded higher shear bond strength than total-etch and multimode/universal adhesives applied in both self-etch and etch-and-rinse techniques.Conclusion: The self-etching/priming system bonded more strongly to the pulpal floor dentin than multimode adhesive and total-etch system.","PeriodicalId":10632,"journal":{"name":"Contemporary Clinical Dentistry","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/49/e9/CCD-14-104.PMC10399809.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9950589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vanishing the Existence of the Mandible? 消失下颌骨的存在？

IF 1.2

Contemporary Clinical Dentistry Pub Date : 2023-04-01 Epub Date: 2023-06-30 DOI: 10.4103/ccd.ccd_545_22

Narayan Dutt Pandey, Shrikant Dusane, Bhushan Ramdas Bhagat, Shubhangi Bhushan Bhagat

引用次数: 0

Rehabilitation and follow up of a Case of Periodontitis - Generalized, Stage IV, Grade B, Progressive, and with no Risk Factors. 一例牙周炎的康复和随访-全身性，IV期，B级，进行性，无危险因素。

IF 1.2

Contemporary Clinical Dentistry Pub Date : 2023-04-01 Epub Date: 2023-06-30 DOI: 10.4103/ccd.ccd_733_21

Sreekanth Puthalath, V C Santhosh, Sameera G Nath, Reshmi Viswanathan

引用次数: 0

The Bond Strength of Root Canal Filling after Calcium Hydroxide Removal with a Simple Apical Negative Pressure Kit in Oval-Shaped Root Canal. 简单根尖负压试剂盒去除氢氧化钙后根管充填在椭圆形根管中的结合强度。

IF 1.2

Contemporary Clinical Dentistry Pub Date : 2023-04-01 Epub Date: 2022-11-11 DOI: 10.4103/ccd.ccd_583_21

Warat Leelapornpisid, Thanapat Sastraruji, Phumisak Louwakul, Anat Dewi

{"title":"The Bond Strength of Root Canal Filling after Calcium Hydroxide Removal with a Simple Apical Negative Pressure Kit in Oval-Shaped Root Canal.","authors":"Warat Leelapornpisid, Thanapat Sastraruji, Phumisak Louwakul, Anat Dewi","doi":"10.4103/ccd.ccd_583_21","DOIUrl":"10.4103/ccd.ccd_583_21","url":null,"abstract":"Background: The aim of this study was to evaluate the effect of a simple irrigating device, which produced from the apical negative pressure concept, as a final irrigating method after calcium hydroxide (CH) medication on the bond strength of epoxy resin-based sealer in the oval-shaped root canal.Methods: Forty-eight single-rooted premolars with oval-shaped canals were included in the study. The crown was decoronized and canal instrumented with Mtwo (VDW GmbH, Munich, Germany) up to size 40/04. The specimens were assigned to four groups according to the irrigation protocol after 1 week of CH medication: control group (no medication) and three experimental groups conventional needle irrigation (CNI), passive ultrasonic irrigation (PUI), and simple apical negative pressure kit (sANP). The teeth were obturated with a warm vertical technique using epoxy resin-based sealers. After 1 week, the roots were transversally sectioned at coronal, middle, and apical thirds. A push-out test was performed by a universal testing machine, and statistical analysis was performed using One-Way ANOVA with Duncan's post hoc test.Results: The bond strength in the control group was significantly higher than the CNI group in all root canal thirds (P < 0.05). At coronal third, sANP showed lower bond strength than PUI group (P < 0.05). However, the bond strength of sANP group was comparable to the control group (P > 0.05) which was significantly higher than the PUI group at apical third (P < 0.05).Conclusion: A sANP enhanced the bond strength of epoxy resin-based sealer in the apical third of CH-medicated root canal in the oval-shaped canal.","PeriodicalId":10632,"journal":{"name":"Contemporary Clinical Dentistry","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/13/81/CCD-14-98.PMC10399799.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9955826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of Smear Layer Removal Efficacy of Neem Leaf Extract, Propolis, and Orange Oil when used as Endodontic Irrigants: An in vitro Scanning Electron Microscopic Study. 印楝叶提取物、蜂胶和橙油用作口腔正畸冲洗剂时去除涂抹层效果的比较评价：体外扫描电子显微镜研究。

IF 1.2

Contemporary Clinical Dentistry Pub Date : 2023-04-01 Epub Date: 2023-06-30 DOI: 10.4103/ccd.ccd_611_21

Ria Setia, Nitika Bajaj, Meenu Bhola, Gurlal Singh Brar

{"title":"Comparative Evaluation of Smear Layer Removal Efficacy of Neem Leaf Extract, Propolis, and Orange Oil when used as Endodontic Irrigants: An in vitro Scanning Electron Microscopic Study.","authors":"Ria Setia, Nitika Bajaj, Meenu Bhola, Gurlal Singh Brar","doi":"10.4103/ccd.ccd_611_21","DOIUrl":"10.4103/ccd.ccd_611_21","url":null,"abstract":"Introduction: In root canal treatment, chemical debridement of the root canal with the help of irrigants is important due to the complex internal anatomy of the teeth. Biomechanical root canal preparation produces a smear layer. It covers the dentinal tubules, which may interfere with the penetration of various irrigants and intracanal medicaments, so the smear layer has to be removed. The potential side effects and certain limitations of chemical irrigants for smear layer removal have led to a shift toward herbal alternatives.Objective: The aim of the present study was to evaluate and compare the smear layer removal efficacy of Neem leaf extract, Propolis, and Orange oil as endodontic irrigants using the scanning electron microscope.Materials and methods: Ninety samples were taken and then randomly (simple random sampling method) divided into three experimental groups (n = 30) depending upon different endodontic irrigants used: Group 1-Neem leaf extract, Group 2-Orange oil and Group 3-Propolis and smear layer removal evaluation was done under the scanning electron microscope Scanning Electron Microscope (SEM) at all the levels, i.e., coronal, middle, and apical.Results: The collected data were analyzed using the Chi-square test. Group 1 (Neem leaf extract) showed the highest smear layer removal efficacy, which was followed by Group 2 (Orange oil) and the least efficacy of smear layer removal was shown by Group 3 (Propolis) at coronal, middle, and apical levels.Conclusion: Group 1 (Neem leaf extract) performed significantly better in removing the smear layer at coronal, middle, and apical levels as compared to the other two groups.","PeriodicalId":10632,"journal":{"name":"Contemporary Clinical Dentistry","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/68/93/CCD-14-128.PMC10399795.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9944370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial and Cytotoxic Activity of Ocimum tenuiflorum and Stevia rebaudiana-Mediated Silver Nanoparticles - An In vitro Study. 细辛和甜叶菊介导的银纳米粒子的抗菌和细胞毒性活性——体外研究。

IF 1.2

Contemporary Clinical Dentistry Pub Date : 2023-04-01 Epub Date: 2022-11-11 DOI: 10.4103/ccd.ccd_369_21

Indumathy Pandiyan, Meignana Indiran Arumugham, Sri Sakthi Doraikannan, Pradeep Kumar Rathinavelu, Jayashri Prabakar, S Rajeshkumar

{"title":"Antimicrobial and Cytotoxic Activity of Ocimum tenuiflorum and Stevia rebaudiana-Mediated Silver Nanoparticles - An In vitro Study.","authors":"Indumathy Pandiyan, Meignana Indiran Arumugham, Sri Sakthi Doraikannan, Pradeep Kumar Rathinavelu, Jayashri Prabakar, S Rajeshkumar","doi":"10.4103/ccd.ccd_369_21","DOIUrl":"10.4103/ccd.ccd_369_21","url":null,"abstract":"Background: Silver nanoparticles (AgNPs) are the nanoparticles of silver between 1 nm and 100 nm in size. In this study, AgNPs were extracted from Ocimum tenuiflorum and Stevia rebaudiana which is a medicinal plant of Indian origin, worshipped by the Hindus and used in Ayurvedic medicine since ancient times.Aim: The aim of the study was to assess the antimicrobial and cytotoxic effect of AgNPs reinforced with the herb O. tenuiflorum and S. rebaudiana against oral pathogens.Materials and methods: In this in vitro study, the organisms used were Streptococcus mutans, Staphylococcus aureus, Lactobacillus sp., and Candida albicans. Agar well-diffusion method was used to assess the antimicrobial efficacy of the nanoparticles at 25 mL, 50 mL, and 100 mL. To assess the cytotoxic effect, brine shrimp lethality assay was used.Results: Zone of inhibition was found to be highest at 100 mL against S. mutans, S. aureus, Lactobacillus sp., and C. albicans. The cytotoxic activity at 5 mL and 10 mL was 0%. The maximum cytotoxicity was seen at 80 mL where 30% of the Nauplii's died.Conclusion: The findings from this study suggest that AgNPs reinforced with O. tenuiflorum and S. rebaudiana extracts has the potential as an antimicrobial agent and has less cytotoxic effect on brime shrimp and can be used as an alternative to commercially available antimicrobial agents.","PeriodicalId":10632,"journal":{"name":"Contemporary Clinical Dentistry","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e9/47/CCD-14-109.PMC10399804.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9953556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Association of Serum Interleukin-10 Level with Glycemic Status to Predict Glycemic Alteration with Periodontitis: A Cross-Sectional, Observational Study. 血清白细胞介素-10水平与血糖状态的相关性预测牙周炎的血糖变化：一项横断面观察研究。

IF 1.2

Contemporary Clinical Dentistry Pub Date : 2023-04-01 Epub Date: 2023-06-30 DOI: 10.4103/ccd.ccd_537_22

S K Aziz Ikbal, Sanjay Gupta, Vandana Tiwari, Gurpreet Dhinsa, Neelu Verma

{"title":"Association of Serum Interleukin-10 Level with Glycemic Status to Predict Glycemic Alteration with Periodontitis: A Cross-Sectional, Observational Study.","authors":"S K Aziz Ikbal, Sanjay Gupta, Vandana Tiwari, Gurpreet Dhinsa, Neelu Verma","doi":"10.4103/ccd.ccd_537_22","DOIUrl":"10.4103/ccd.ccd_537_22","url":null,"abstract":"Background and aim: Studies showed bidirectional relationship between chronic periodontitis (CPD) and diabetes. This study was conducted to estimate the levels of serum interleukin (IL)-10 in patients with CPD and type 2 diabetes mellitus (T2DM) to evaluate the association of serum IL-10 levels with glycemic status and to evaluate the influence of periodontal inflammation on glycemic control.Setting and design: A cross-sectional observational study.Materials and methods: Eighty patients were divided into four groups consisting of 20 patients in each group. Group 1: healthy control, Group 2: T2DM without chronic periodontitis, Group 3: chronic periodontitis only (CPD), and Group 4: T2DM with chronic periodontitis (CPD). Plaque index, gingival index, pocket probing depth, clinical attachment loss, random blood sugar, and glycated hemoglobin (HbA1c) level were recorded for categorizing patients into aforestated groups. Serum IL-10 level was measured by ELISA kit.Statistical analysis used: ANOVA statistics and post hoc Tukey's test were performed for comparing individual groups.Results: IL-10 was detected lowest in Group 3 followed by Group 4. Highest level of IL-10 was found in the healthy group then in Group 2.Conclusion: IL-10 levels have an inverse relationship with HbA1c. Lowest level of IL-10 in CPD dictates periodontal inflammation itself influences in regulating serum IL-10 level and poor glycemic control. Serum IL-10 level may be one of the predictors of glycemia.","PeriodicalId":10632,"journal":{"name":"Contemporary Clinical Dentistry","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/65/8f/CCD-14-152.PMC10399803.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9955827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioimpedance: A Tool for Screening Oral Cancer - A Systematic Review. 生物阻抗：筛查口腔癌症的工具-系统综述。

IF 1.2

Contemporary Clinical Dentistry Pub Date : 2023-04-01 Epub Date: 2023-06-30 DOI: 10.4103/ccd.ccd_195_23

Vaibhav Gupta, Usha Agrawal, Poonam Goel

{"title":"Bioimpedance: A Tool for Screening Oral Cancer - A Systematic Review.","authors":"Vaibhav Gupta, Usha Agrawal, Poonam Goel","doi":"10.4103/ccd.ccd_195_23","DOIUrl":"10.4103/ccd.ccd_195_23","url":null,"abstract":"Objective: The successful management of cancer depends on proper screening and treatment methods. Bioimpedance spectroscopy (BIS) is an established technique in detecting breast cancer, cervical cancer, and prostate cancer. This systematic review sought to investigate the current evidence regarding the clinical application of bioimpedance in the detection of oral squamous cell carcinoma and oral potentially malignant disorders.Study design: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were followed to perform this review. Electronic databases such as PubMed, MEDLINE, Embase, EBSCOhost, and Google Scholar were searched till March 2022. Articles published in the English medical literature on human participants report on the application of BIS in the screening of precancerous and cancerous lesions. The primary endpoint was defined as the ability to differentiate between normal and cancerous tissue.Results: A total of 6754 articles were identified; of which 481 were eligible for inclusion. Only five articles met the eligibility criteria and were included in the study. Qualitative analysis for each study was done to assess the data provided. All the studies demonstrated a significant divergence in BIS metrics between cancerous and normal tissue at 20 Hz and 50 KHz.Conclusion: Bioimpedance appears to be a promising novel tool for the detection of various malignancies which can be used in community screening due to its noninvasiveness and portability.","PeriodicalId":10632,"journal":{"name":"Contemporary Clinical Dentistry","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/c8/CCD-14-91.PMC10399808.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9950584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Antibacterial and Antibiofilm Effect of Amoxicillin and Mangifera indica L. Leaves Extract on Oral Pathogens. 阿莫西林和芒果叶提取物对口腔病原菌的抗菌和抗菌膜作用。

IF 1.2

Contemporary Clinical Dentistry Pub Date : 2023-04-01 Epub Date: 2023-06-30 DOI: 10.4103/ccd.ccd_399_22

Sheila Soesanto, Evangelista Rachel Hepziba, Yasnill, Armelia Sari Widyarman

{"title":"The Antibacterial and Antibiofilm Effect of Amoxicillin and Mangifera indica L. Leaves Extract on Oral Pathogens.","authors":"Sheila Soesanto, Evangelista Rachel Hepziba, Yasnill, Armelia Sari Widyarman","doi":"10.4103/ccd.ccd_399_22","DOIUrl":"10.4103/ccd.ccd_399_22","url":null,"abstract":"Objective: This study aimed to determine the antibacterial and antibiofilm effects of amoxicillin combined with extract of Mangifera indica L. leaves against Staphylococcus aureus and Porphyromonas gingivalis.Materials and methods: This was an experimental laboratory in vitro study with a posttest-only control group design. An antibacterial test using the plate count method and an antibiofilm test using the microtiter plate biofilm assay method were conducted. The research samples comprised extract of M. indica L. leaves with a concentration of 100%; amoxicillin and extract concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, and 100%; and amoxicillin. Dimethyl sulfoxide served as a negative control and co-amoxiclav served as a positive control.Results: The combination of amoxicillin and the extract exhibited an antibacterial effect against S. aureus at a concentration of 12.5% and higher and more effective than co-amoxiclac P. gingivalis at a concentration of 3.125% and higher. In the antibiofilm test, the combination of amoxicillin and the extract at a concentration of 25% after 1 h of incubation and a concentration of 6.25% after 3 h of incubation inhibited S. aureus. The inhibition of S. aureus biofilms at a concentration of 100% after 24 h of incubation was as effective as that of co-amoxiclav. The extract at a concentration of 25% over the entire incubation period showed more potent inhibition against the P. gingivalis biofilm than co-amoxiclav.Conclusions: The ethanolic extract of M. indica L. leaves and the combination of amoxicillin and the extract have the potential to inhibit the growth and formation of S. aureus and P. gingivalis biofilms.","PeriodicalId":10632,"journal":{"name":"Contemporary Clinical Dentistry","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0d/b4/CCD-14-145.PMC10399797.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9950590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Evaluation of Cytotoxic Effects of Methanolic Leaf Extracts of Annona muricata on Oral Squamous Cell Carcinoma-15 Cell Lines and its Effect On Expression of Bcl 2-associated X protein, B-cell C/Lymphoma 2 and p53 Genes 番荔枝叶甲醇提取物对口腔鳞癌-15细胞株细胞毒作用的体外评价及其对Bcl - 2相关X蛋白、b细胞C/淋巴瘤2和p53基因表达的影响

Contemporary Clinical Dentistry Pub Date : 2023-01-01 DOI: 10.4103/ccd.ccd_81_23

S. Jeslin Mary, Veeran Veeravarmal, Isaac Joseph Tharmasahayam, Percy Ida Augustine, D. Angelin, Mohamed Hanifa Mohamed Nassar

{"title":"In vitro Evaluation of Cytotoxic Effects of Methanolic Leaf Extracts of Annona muricata on Oral Squamous Cell Carcinoma-15 Cell Lines and its Effect On Expression of Bcl 2-associated X protein, B-cell C/Lymphoma 2 and p53 Genes","authors":"S. Jeslin Mary, Veeran Veeravarmal, Isaac Joseph Tharmasahayam, Percy Ida Augustine, D. Angelin, Mohamed Hanifa Mohamed Nassar","doi":"10.4103/ccd.ccd_81_23","DOIUrl":"https://doi.org/10.4103/ccd.ccd_81_23","url":null,"abstract":"Abstract Background: Due to their wide spectrum of phytochemical components and lack of side effects, the use of plants for the prevention and treatment of cancer has recently attracted increased attention. One among them is Annona muricata , commonly called soursop. According to recent investigations, several types of cancer have been successfully treated using this plant’s extracts. However, studies on oral squamous cell carcinoma (SCC) are very limited. Aim: In the present study, we aimed to investigate the cytotoxic potential of leaf extract of A. muricata (LEAM) against oral tongue SCC-15 cell lines, using in vitro assays. Materials and Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide assay was performed to assess cytotoxic activity, and the apoptotic effect was determined using gene expression analyses of Bcl 2-associated X protein (Bax), B-cell C/lymphoma 2 (Bcl-2), and tumor-suppressor phosphoprotein (p53). Results: Significant cytotoxicity ( P ≤ 0.05) with a minimum inhibitory concentration value of 40 μg/ml was observed with the LEAM on SCC-15 cell lines. A highly significant decrease was observed in Bcl-2 gene expression ( P < 0.05), whereas p53 and BAX genes revealed a highly significant increase ( P < 0.05) when SCC-15 cell lines were treated with LEAM in the study group compared to the control. Conclusion: These results show that LEAM has the potential for development as a therapeutic agent for cytotoxicity, particularly on oral SCC cells, following further investigation.","PeriodicalId":10632,"journal":{"name":"Contemporary Clinical Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134980222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0