Community Based Medical Journal最新文献

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Should Physicians Avoid Routine Iron Supplementation in Rheumatic Musculoskeletal Diseases (RMDs)? 风湿病肌肉骨骼疾病(RMDs)患者应避免常规补铁吗?
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64239
Mohammad Aminul Islam
{"title":"Should Physicians Avoid Routine Iron Supplementation in Rheumatic Musculoskeletal Diseases (RMDs)?","authors":"Mohammad Aminul Islam","doi":"10.3329/cbmj.v12i1.64239","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64239","url":null,"abstract":"Abstract not available \u0000CBMJ 2023 January: Vol. 12 No. 01 P: 01-02","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"2146 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86555499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study on the Effect of Topical Pre-operative Nonsteroidal Anti-inflammatory Drugs in Maintaining Mydriasis during Small Incision Cataract Surgery 术前局部非甾体类抗炎药对小切口白内障手术维持瞳孔散瞳效果的研究
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64276
Md Emdad Hussain
{"title":"Study on the Effect of Topical Pre-operative Nonsteroidal Anti-inflammatory Drugs in Maintaining Mydriasis during Small Incision Cataract Surgery","authors":"Md Emdad Hussain","doi":"10.3329/cbmj.v12i1.64276","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64276","url":null,"abstract":"A prospective, randomized, double masked, single centered comparative study done in the Bangladesh Naval Ship Patenga, Chattogram, Bangladesh, from July 2021 to June 2022, to assess the clinical benefits and effectiveness of prophylactic pre-operative topical administration of NSAIDs in maintaining mydriasis during small incision cataract surgery. Thirty patients underwent small incision cataract surgery with intra ocular lens implantation were randomized to compare the efficacy of topical NSAIDs drops (Group-A) and placebo drops (Group-B) in maintaining peroperative mydriasis. Balanced salt solution containing non preservative adrenaline was used in all patients. Pupil size was measured prior to the corneal incision and after the completion of the operation. The two groups were analyzed with respect to the changes in pupillary diameter at the beginning and at the end of the operation. The change in pupil size was significantly different among the two groups at the end of the surgery. The mean vertical and horizontal diameter of the two groups were near about similar at the beginning of the surgery. Significant differences were seen after IOL implantation with the NSAIDs group having the larger mean diameter in both vertical (P=0.003) and horizontal (P=0.008) pupillary measurements at the conclusion of surgery. Topical NSAIDs has been shown to be a more effective inhibitor of meiosis during per operative uncomplicated small incision cataract surgery and provides a more stable mydriatic effect during surgery compared to the placebo group. \u0000CBMJ 2023 January: Vol. 12 No. 01 P: 22-26","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87061676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Health Literacy: Intervention and Outcome in Child Healthcare in Bangladesh 健康素养:孟加拉国儿童保健的干预和结果
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64396
S. Sharmin, A. S. M. Nurunnabi, Shamsi Sumaiya Ashique, Asmay Jahan, Munira Begum
{"title":"Health Literacy: Intervention and Outcome in Child Healthcare in Bangladesh","authors":"S. Sharmin, A. S. M. Nurunnabi, Shamsi Sumaiya Ashique, Asmay Jahan, Munira Begum","doi":"10.3329/cbmj.v12i1.64396","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64396","url":null,"abstract":"Bangladesh made significant improvements in child healthcare and achieved its Millennium Development Goal (MDG) 4 (to reduce child mortality). In 2010, the United Nations recognized Bangladesh for its exceptional progress towards MDG 4 to reduce child and maternal mortality in the face of many socioeconomic challenges. We are also committed to achieve our Sustainable Development Goal (SDG) by 2030. Health education department of Government of Bangladesh prioritized several essential health interventions over the past decades. There were focuses on expanding immunization, tackling diarrhoea and pneumonia, vitamin A campaign, prevention of blindness, iodine deficiency, and improving adolescent health. These programmes and strategies were scaled-up nationwide. The government along with its stakeholders promoted widespread availability and affordability of those health literacy interventions with an emphasis on community-based approaches. As a result, inequities in coverage of specific interventions declined; this may explain the improvements seen amongst even the most disadvantaged populations of the country. Media campaigns played a great role, which were undertaken in Bangladesh for decades and involved a nationally broadcast television supported by radio, television, newspaper, and billboard advertisements and local promotion activities. We expect that ongoing public private partnerships (PPPs) in which the government contracts NGOs and the private sector will enable us greater success and coverage of health information and literacy services throughout the country. \u0000CBMJ 2023 January: Vol. 12 No. 01 P: 124-128","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88920269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Renal Impairment in a Patient with Multiple Myeloma: A Case Report 多发性骨髓瘤患者肾脏损害1例报告
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64374
Mahmud Hasan, N. C. Ray
{"title":"Renal Impairment in a Patient with Multiple Myeloma: A Case Report","authors":"Mahmud Hasan, N. C. Ray","doi":"10.3329/cbmj.v12i1.64374","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64374","url":null,"abstract":"Multiple myeloma (MM) is a type of cancer occurs due to malignant plasma cell that affects with various clinical manifestations including renal impairment, anemia, bone disease and infection. The most common renal presentation of multiple myeloma is myeloma cast nephropathy. We described a case of multiple myeloma along with renal impairment in a 62-year old male patient. His serum B2-Microglobulin was 19.18 mg/L, which is much higher than reference value. His serum creatinine was 2.58 mg/dL. After continuing medication, the patient’s renal function was restored. This case is being reported for clinical knowledge dissemination and building clinical insight. \u0000CBMJ 2023 January: Vol. 12 No. 01 P: 111-114","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72691858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Incidence and Risk Factors of Congenital Heart Diseases in Syndromic Neonates 综合征新生儿先天性心脏病发病率及危险因素分析
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64272
Md Mahbubur Rahman, Abdullah Al Amin, J. Ferdaus, M. Quader
{"title":"Incidence and Risk Factors of Congenital Heart Diseases in Syndromic Neonates","authors":"Md Mahbubur Rahman, Abdullah Al Amin, J. Ferdaus, M. Quader","doi":"10.3329/cbmj.v12i1.64272","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64272","url":null,"abstract":"Congenital heart defects (CHD) are the most common types of major birth defects, leading cause of infant morbidity and mortality. Syndromic babies are more affected by CHD. This observational study was conducted in a tertiary care hospital of military facility in Bangladesh. Eighty syndromic neonates were studied through history, physical examination and investigated for incidence and risk factors for CHD. Out of 80 syndromic neonates 61(76.3%) were found having CHD. Male female ratio was 1.18:1. Average age at diagnosis was 25±1.6 days of age. Down Syndrome (DS) and Congenital Rubella Syndrome (CRS) were the most common syndromes. Ventricular Septal defect (VSD) and Atrial septal defect (ASD) II were the most common CHD. Consanguinity, extracardiac malformations, maternal illness (HTN, DM, Hypothyroidism etc.), both fertility and non-fertility medication are also found to be associated with CHD in Syndromic neonates. CHDs have serious implications and it is important to understand them and how they may affect the child so that appropriate medical and surgical treatment may be provided. So, early detection of CHD in neonate will be able to decrease morbidity as well as mortality.\u0000CBMJ 2023 January: Vol. 12 No. 01 P: 17-21","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81529647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison Between Pethidine and Nalbuphine Hydrochloride Combating Epigastric Pain During Mopping of Intraperitoneal Blood in Lower Uterine Caesarean Section Operation 哌替啶与盐酸纳布啡对抗下子宫剖宫产术腹膜内吸血时胃脘痛的比较
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64362
Md. Ashraful Islam, S. Akter, M. Bashar, Md Abdullah Tareq Bhuiyan, Evana Samad, Md. Abu Kawsar, M. Hossain, Taneem Mohammad, Shuchana Chakma, Hasnat Khan
{"title":"Comparison Between Pethidine and Nalbuphine Hydrochloride Combating Epigastric Pain During Mopping of Intraperitoneal Blood in Lower Uterine Caesarean Section Operation","authors":"Md. Ashraful Islam, S. Akter, M. Bashar, Md Abdullah Tareq Bhuiyan, Evana Samad, Md. Abu Kawsar, M. Hossain, Taneem Mohammad, Shuchana Chakma, Hasnat Khan","doi":"10.3329/cbmj.v12i1.64362","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64362","url":null,"abstract":"A randomized prospective single blind trial was conducted in the Department of Anaesthesia, Analgesia, Palliative and Intensive Care Medicine, Dhaka Medical College Hospital, Dhaka, Bangladesh, between July 2021 and February 2022, to compare the effects of intravenous pethidine and nalbuphine hydrochloride combating epigastric pain and discomfort during mopping of intraperitoneal blood in lower uterine caesarean section (LUCS) operation. A total of 120 women participated in the study. They were randomly divided into two groups: group A (n=60) received intravenous diluted (with normal saline) 20-25 mg of pethidine, while group B (n=60) received intravenous diluted 10-20 mg of nalbuphine hydrochloride. There were no differences observed in mean age, weight, height, and duration of operation between two groups (P>0.05). However, participants of group B reported less or no epigastric pain and discomfort during mopping of intraperitoneal blood than that of group A (P<0.05), i.e., episode and intensity of pain measured by using visual analogue scales indicated that nalbuphine hydrochloride has a longer duration of action than pethidine. Our study revealed that intravenous diluted nalbuphine hydrochloride works better than pethidine to minimize epigastric pain and discomfort during mopping of intraperitoneal blood in lower uterine caesarean section (LUCS) operation.   \u0000CBMJ 2023 January: Vol. 12 No. 01 P: 81-86","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81953328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correlation of Serum Vitamin D and Calcium Levels between Outdoor and Indoor Working Professionals in Dhaka City, Bangladesh 孟加拉国达卡市室外和室内职业人员血清维生素D和钙水平的相关性
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64363
Tarana Tasrin, Shahnila Ferdousi, Md Kamal Sultan, M. Mozaffor
{"title":"Correlation of Serum Vitamin D and Calcium Levels between Outdoor and Indoor Working Professionals in Dhaka City, Bangladesh","authors":"Tarana Tasrin, Shahnila Ferdousi, Md Kamal Sultan, M. Mozaffor","doi":"10.3329/cbmj.v12i1.64363","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64363","url":null,"abstract":"A cross-sectional study was conducted in the Department of Biochemistry, Dhaka Medical College, Dhaka, Bangladesh, from July 2018 to June 2019, to compare serum vitamin D levels and observe its correlation with serum calcium levels among the outdoor and indoor working professionals. A total of 101 indoor and outdoor workers of different sectors living in Dhaka city participated in this study. All study subjects were divided into two groups: outdoor workers were in group A (n=35) and indoor workers were in group B (n=66). Estimation of serum vitamin D3 was done by chemiluminescent microparticle immunoassay, while serum calcium was measured using Arsenazo-III dye method. The mean age of the study participants was 38.72±6.88 years, ranging between 25 and 52 years. Among the study participants, 64(63.4%) were male and 37(36.6%) were female. Among outdoor workers, most were from Traffic Police Services (83%) and the rest were street hawkers (17%). Indoor workers were recruited from the hospital – nurses (32%), doctors (27%), administrative employees (21%), hospital ward assistants (12%) and medical technicians (8%). Serum vitamin D3 level was found higher in outdoor workers than that of indoor workers (19.35±5.67 ng/ml vs. 14.17±4.18 ng/ml) and the difference was statistically significant (P<0.001). Serum calcium level was lower in outdoor workers than that of indoor workers (8.82±0.54 mg/dl vs. 9.08±1.08 mg/dl); however, the difference was not statistically significant (P>0.05). Moreover, no significant correlation was observed between serum vitamin D and calcium levels in both outdoor (r= – 0.117; P=0.510) and indoor (r= – 0.163; P=0.196) groups. \u0000CBMJ 2023 January: Vol. 12 No. 01 P: 87-92","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83203021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
When Small Bugs Cause Big Problems: Tropical Insect Bite Led to Myocarditis 当小虫子引起大问题:热带昆虫叮咬导致心肌炎
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64373
Sm Ahsanul Habib, Taneem Mohammad, M. A. Alam
{"title":"When Small Bugs Cause Big Problems: Tropical Insect Bite Led to Myocarditis","authors":"Sm Ahsanul Habib, Taneem Mohammad, M. A. Alam","doi":"10.3329/cbmj.v12i1.64373","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64373","url":null,"abstract":"Wasp bite induced allergic reactions or organ dysfunctions are not uncommon in tropical countries. Most people affected with insect bite hardly develop any signs or symptoms; however, about 30 percent of them may show signs of illness which is often overlooked and causes fatality. Among the fatal complications specially anaphylaxis and renal dysfunction have been reported; however, myocardial involvement in the form of myocarditis is very rare. Awareness about such complications is important as these affect the clinical course and outcomes of the patients. We have presented a case of wasp bite induced myocarditis and heart failure here, which was diagnosed at a later stage as patient did not improve being admitted in the emergency department and subsequently transferred to the critical care unit.  \u0000CBMJ 2023 January: Vol. 12 No. 01 P: 107-110","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75330173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison between Effects of Midazolam and Dexmedetomidine as Sedative in Elective Caesarean Section under Spinal Anaesthesia 咪达唑仑与右美托咪定在脊髓麻醉下择期剖宫产术中的镇静效果比较
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64365
M. E. Karim, Ram Mustafijur Rashid, Mohammad Saleh Akram Akram, R. Ershad
{"title":"Comparison between Effects of Midazolam and Dexmedetomidine as Sedative in Elective Caesarean Section under Spinal Anaesthesia","authors":"M. E. Karim, Ram Mustafijur Rashid, Mohammad Saleh Akram Akram, R. Ershad","doi":"10.3329/cbmj.v12i1.64365","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64365","url":null,"abstract":"This randomized clinical trial compares midazolam and dexmedetomidine in terms of onset and recovery of sedation, haemodynamic effects, respiratory effects, and adverse effects of both the drugs in elective Caesarian section under spinal anaesthesia. The study included 60 ASA grade-I patients between age 20- and 40-years undergoing elective Caesarean sections under subarachnoid anaesthesia, from January 2022 to June 2022. Patients were randomly allocated to one of the two groups: midazolam group (Group-I, n=30), who received midazolam in a single dose of 0.10mg/kg and Dexmedetomidine group (Group-II, n=30), who received dexmedetomidine in a single dose of 2mcg/kg. Spinal anaesthesia was conducted by injecting a hyperbaric solution of 0.5% bupivacaine 3ml through a 25G spinal needle at L3-4 level. All parameters were documented at 5-minute intervals until arousal of the patient. The onset of sedation i.e., time from IV (intravenous) injection of Midazolam or Dexmedetomidine to closure of eye lids (OAA/S score of 3) and the arousal time from sedation i.e., time from closing of the eye lids to OAA/S score of 5 (patient is awake clinically) were noted. Any complication during operation was documented. The patient’s satisfaction with the sedation was assessed by the 5-point ‘Likert verbal rating scale’. There was no significant difference of mean blood pressure and mean heart rate between the two groups at different time intervals (P>0.05). Time of onset of sedation was significantly delayed in dexmedetomidine group (P<0.05). Duration of sedation was comparable between the two groups (P>0.05). Incidence of peroperative complications were comparable between the two groups (P>0.05). Haemodynamic effects and adverse effects of two drugs were comparable. Therefore, it is recommended that either midazolam or dexmedetomidine can be used for sedation in single dose technique during subarachnoid block for Caesarean section. \u0000CBMJ 2023 January: Vol. 12 No. 01 P: 99-106","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81225262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenotypic and Genotypic Characterization of Salmonella Typhi isolated from Human Blood with Distribution of Virulence Genes and Sequencing 人血伤寒沙门菌的表型和基因型鉴定、毒力基因的分布和测序
Community Based Medical Journal Pub Date : 2023-02-19 DOI: 10.3329/cbmj.v12i1.64287
Nigha Zannat Dola, S. Shamsuzzaman, M. Islam, A. Rahman, Nafisa Jabin Mishu, Modina Ansary Nabonee, R. A. Jalil
{"title":"Phenotypic and Genotypic Characterization of Salmonella Typhi isolated from Human Blood with Distribution of Virulence Genes and Sequencing","authors":"Nigha Zannat Dola, S. Shamsuzzaman, M. Islam, A. Rahman, Nafisa Jabin Mishu, Modina Ansary Nabonee, R. A. Jalil","doi":"10.3329/cbmj.v12i1.64287","DOIUrl":"https://doi.org/10.3329/cbmj.v12i1.64287","url":null,"abstract":"A cross-sectional study was conducted in the Department of Microbiology at tertiary care hospital in Bangladesh from July 2019 to June 2020, to see the phenotypic and genotypic characterization of Salmonella Typhi isolated from human blood with distribution of virulence genes and sequencing. Clinically suspected enteric fever patients irrespective of age and gender who attended either the Department of Microbiology or Medicine out-patient department (MOPD) in a tertiary level hospital in Bangladesh for blood culture and sensitivity test were included in this study. The positive growth S. Typhi was identified by gram straining, colony morphology and biochemical test. Then Salmonella Typhi was identified by using Salmonella specific antisera. Final identification was done by using 16s Rrna by PCR. Virulence factors like hemolysin and siderophore was detected by siderophores production assay. PCR and sequencing of virulence genes were done. A total number of 50 isolated organisms were identified as Salmonella species of which Salmonella Typhi was detected in 40 (48.2%) isolates. Among 40 isolated Salmonella Typhi, no Salmonella Typhi produced hemolysin and 30 (75.0%) isolates have ability to produce siderophore. Among the isolated Salmonella Typhi all isolates 40 (100%) were positive for viaB and staA gene, 21 (52.5%) were positive for iroB. Sequencing of viaB and iroB were done for further validation. In conclusion all isolated Salmonella Typhi carried viaB and staA, iroB genes were responsible for S. Typhi pathogenicity. \u0000CBMJ 2023 January: Vol. 12 No. 01 P: 41-47","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78967295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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