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Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie最新文献

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Heterogeneity of microfibrils: role of thrombospondin-microfibrils in the thrombogenicity of the subendothelium. 微纤维的异质性:血栓反应-微纤维在内皮下层血栓形成中的作用。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-04-01
F Fauvel-Lafève, B Arbeille, C de Romeuf, M Lemesle, Y J Legrand
{"title":"Heterogeneity of microfibrils: role of thrombospondin-microfibrils in the thrombogenicity of the subendothelium.","authors":"F Fauvel-Lafève,&nbsp;B Arbeille,&nbsp;C de Romeuf,&nbsp;M Lemesle,&nbsp;Y J Legrand","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We report the results of an immunogold electron microscopical analysis on microfibrils from the arterial subendothelium showing that thrombospondin (TSP) is present on 40 nm-diameter structures joining 8-10 nm-diameter microfibrils containing fibrillin. They differ from type VI collagen which forms 3-5 nm-diameter microfibrils. TSP containing microfibrils (TSP-MF) extracted from human umbilical arteries did not contain fibrillin or type VI collagen. Blood platelet interactions with TSP-MF were not modified by anti-fibrillin or anti-type VI collagen antibodies. In situ, vWF was bound to cross-linked microfibrils, at the level of their 40 nm junction, and a double-labeling with the anti-thrombospondin and anti-vWF antibodies was observed. In vitro, vWF binding to TSP-MF was not inhibited by anti-fibrillin or anti-type VI collagen antibodies. These results suggest a structural and functional heterogeneity of microfibrils and emphasize the role of TSP-MF in the thrombogenicity of the subendothelium.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 4","pages":"333-41"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19735914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microanalysis of serotonin by high pressure liquid chromatography with electrochemical detection. 电化学检测高压液相色谱微量分析血清素。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-04-01
P Nirdé, N Nagel
{"title":"Microanalysis of serotonin by high pressure liquid chromatography with electrochemical detection.","authors":"P Nirdé,&nbsp;N Nagel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Serotonin (5-HT) analysis by high pressure liquid chromatography with electrochemical detection (HPLC-ED) has been designed to perform micro assays below the picogram range. We demonstrated that the oxidation potential of 5-HT was tightly linked to the pH of the buffer used as eluent phase. The electrochemistry of 5-HT in pH 4.00 acetate buffer and in pH 6.80 phosphate buffer allowed quantification down to 2 x 10(-18) g. The oxidization current was linked to the 5-HT amount added. The electrochemical response of 5-HT was different for low amounts in the 10(-18) g range and for amounts above the picogram range. Two curves were obtained according to the electrochemical response of 5-HT: the first one was for the 10(-18) g range and the second one was for the picogram range and above. It thus appeared between concentration ranges that assays are not be feasible. This method has been used to analyse 20 microns frozen slices of rat spinal cord. These data open new insights into scarce quantification of 5-HT without the use of radio-elements.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 4","pages":"249-55"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19734660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological indications of hair cell neodifferentiation in the organ of Corti of amikacin treated rat pups. 阿米卡星处理大鼠幼鼠Corti器官毛细胞新分化的形态学特征。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-04-01
M Lenoir, P Vago
{"title":"Morphological indications of hair cell neodifferentiation in the organ of Corti of amikacin treated rat pups.","authors":"M Lenoir,&nbsp;P Vago","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Postnatal rats were treated with amikacin during the period of cochlear supra-normal sensitivity to ototoxic antibiotics, i.e. from day 9 to day 16 when the organ of Corti is achieving its maturation. Scanning and transmission electron microscopies were used to evaluate morphological changes in the organs of Corti at different post-treatment periods, up until postnatal day 90. A massive loss in outer (OHCs) and inner (IHCs) hair cells was observed from as early as day 17. A prominent feature in the apical part of cochleas at days 21 and 35 was the transient presence of small atypical cells in the region of pre-existing OHCs. These atypical cells did not have the appearance of degenerating OHCs, but had tuffs of microvilli reminiscent of nascent stereociliary bundles. A second striking observation was the presence of pear-shaped cells in the place normally occupied by IHCs. These cells had neither cuticular plate nor stereocilia, but their basal pole was contacted by both afferent and efferent fibers, as in the early stages of IHC maturation. Such efferent contacts are very rare in the adult cochlea. These findings, not previously reported in antibiotic poisoned cochleas of adult animals, suggest that an attempt at sensory cell neodifferentiation occurs in vivo in the developing organ of Corti intoxicated with aminoglycoside antibiotics.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 4","pages":"269-76"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19735909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Performance of urea-sensitive enzyme field effect transistors: influence of the storage conditions. 脲敏感酶场效应晶体管的性能:贮存条件的影响。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-04-01
S Poyard, D Gorchkov, A Jdanova, N Jaffrezic-Renault, C Martelet, A Soldatkin, A El'Skaya
{"title":"Performance of urea-sensitive enzyme field effect transistors: influence of the storage conditions.","authors":"S Poyard,&nbsp;D Gorchkov,&nbsp;A Jdanova,&nbsp;N Jaffrezic-Renault,&nbsp;C Martelet,&nbsp;A Soldatkin,&nbsp;A El'Skaya","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Urea-sensitive enzyme field effect transistors (ENFETs) were prepared by cross-linking urease with bovine serum albumin in saturated glutaraldehyde vapor on the sensitive surface of a pH-FET. The linear part of the biosensor dynamic range is between 5 x 10(-5) and 10(-3) M of urea. The influence of pH on the sensor response, stability and reproducibility of the urea sensor were examined. The addition of EDTA, glycerol, sodium azide and dithiothreitol in the storage buffer solution was studied. In these specific storage conditions, an increase of sensor sensitivity and stability was observed, which means that the enzyme is inhibited during the immobilisation procedure and can be partially restored. Furthermore such reagents do not affect the operational characteristics of the sensor when working in serum.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 4","pages":"257-62"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19734661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Method of measurement of cadmium influx by fura-2 titration in MDCK cell evidenced. Fluorescence video-microscopy study]. [证实了用fura-2滴定法测定MDCK细胞中镉内流的方法。]荧光视频显微镜研究]。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-04-01
M Grouselle, A Boudou, B Oreja-Erroz
{"title":"[Method of measurement of cadmium influx by fura-2 titration in MDCK cell evidenced. Fluorescence video-microscopy study].","authors":"M Grouselle,&nbsp;A Boudou,&nbsp;B Oreja-Erroz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cadmium influx rate in mammal kidney cells (MDCK) is analyzed using an original method based on fura-2 titration. The method relies on the high affinity of the fluorophore for the metal. It follows that the excitation spectrum shift of fura-2 can be linearly correlated to the influx rate of cadmium. Fluorescence digital imaging microscopy allows the study at single cell and intra-cellular organite levels. Results show that the cadmium uptake seems to be carrier dependent. Metal fluxes are potential independent, with a temperature effect caracterized by a Q10 of 2.3 +/- 0.2. No effect of verapamil is noticed; however, cadmium transport is inhibited by external calcium. Apparent dissociation constant for the cadmium uptake is estimated at 4.5 10(-5) M at 20 degrees C. An additional passive transmembrane diffusion process is also evidenced.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 4","pages":"277-87"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19735910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dictyopterin appears at amoebae emergence during spore germination in Dictyostelium. 在竹荪孢子萌发过程中，变形虫萌发时会出现双蝶呤。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-04-01
R Klein, I Tatischeff, D Cotter
{"title":"Dictyopterin appears at amoebae emergence during spore germination in Dictyostelium.","authors":"R Klein, I Tatischeff, D Cotter","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Intracellular dictyopterin, which is present in reduced form, mainly as tetrahydrodictyopterin, both in vegetative cells and in starved cells, could neither be detected in dormant spores nor in unswollen or swollen activated spores. Synthesis of tetrahydrodictyopterin starts with emergence of nascent amoebae during spore germination. Tetrahydrodictyopterin levels in cell-free extracts vary as a function of the percent emerged amoebae in spore suspension. These data are consistent with a requirement for tetrahydrodictyopterin during vegetative growth.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 4","pages":"289-94"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19735911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Skeletochronological study in Bufo bufo in Brittany]. [布列塔尼Bufo Bufo的骨骼年代研究]。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-04-01
T Frétey, B Le Garff
{"title":"[Skeletochronological study in Bufo bufo in Brittany].","authors":"T Frétey,&nbsp;B Le Garff","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study on common toad (Bufo bufo), demonstrates the identity of structure between femur and phalange of a same individual. That allowed us to use skeletochronology without killing animals, and to study the same population. The periodicity of the lines of arrested growth (LAG) is annual. The absolute age is determined in 96.2% of males and 87.8% of females. The endosteal resorption shows a sexual dimorphism, more important for females. The highest longevity recorded in the sample is 12 years for males and 9 years for females. We have determined of the precise age at which individuals have obtained their sexual maturity for 92.9% of the males and 85.4% of the females. The number of lines of arrested growth (LAG) resorbed does not increase with age after adulthood.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 4","pages":"295-9"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19735912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Expression of growth dynamic in the structure of periosteal bone in Anas platyrhynchos]. [长颈海鱼骨膜结构生长动态的表达]。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-04-01
J Castanet, A Grandin, A Abourachid, A de Ricqlès
{"title":"[Expression of growth dynamic in the structure of periosteal bone in Anas platyrhynchos].","authors":"J Castanet,&nbsp;A Grandin,&nbsp;A Abourachid,&nbsp;A de Ricqlès","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An experimental study of the periosteal bone growth in the mallard from 42 to 154 post hatching days shows: (1) a noticeable time difference in the local biological age of the diaphyseal cortices between various long bones; (2) great differences in their histological structures, at a given individual age, expressing commensurate differences in local growth rates. Those results emphasize the importance of local factors to interpret the typology of the primary (periosteal) bone tissues. Experimental results allow to quantify the relationships between bone tissue typology and the velocity of its radial deposition.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 4","pages":"301-8"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19735913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systematics of Euromediterranean Silene (Caryophyllaceae): evidence from a phylogenetic analysis using ITS sequences. 欧洲地中海石楠属(石楠科)的系统学:基于ITS序列的系统发育分析证据。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-04-01
C Desfeux, B Lejeune
{"title":"Systematics of Euromediterranean Silene (Caryophyllaceae): evidence from a phylogenetic analysis using ITS sequences.","authors":"C Desfeux,&nbsp;B Lejeune","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The generic boundaries within the sub-family Silenoideae (Caryophyllaceae), and especially within the complex Silene-Lychnis-Melandrium have long been controversial. These 3 genes are now grouped in a single genus: Silene. In order to test the monophyly of this genus and to clarify intra-generic phylogenetic relationships, we compared ITS sequences of 22 species of Euromediterranean Silene chosen throughout the classification, and 4 putative outgroup species. The genus Silene in its current circumscription formed a well-supported monophyletic group. Lychnis and Melandrium species are scattered within the genus Silene. The monotypic genus Cucubalus also fell within the Silene clade.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":" ","pages":"351-8"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27358529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Database cloning human delta 1-pyrroline-5-carboxylate synthetase (P5CS) cDNA: a bifunctional enzyme catalyzing the first 2 steps in proline biosynthesis. 数据库克隆人类δ 1-吡咯-5-羧酸合成酶(P5CS) cDNA:催化脯氨酸生物合成前2步的双功能酶。
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-03-01
B Aral, J S Schlenzig, G Liu, P Kamoun
{"title":"Database cloning human delta 1-pyrroline-5-carboxylate synthetase (P5CS) cDNA: a bifunctional enzyme catalyzing the first 2 steps in proline biosynthesis.","authors":"B Aral,&nbsp;J S Schlenzig,&nbsp;G Liu,&nbsp;P Kamoun","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>delta 1-pyrroline-5-carboxylate synthetase (P5CS) catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis. We cloned a human P5CS cDNA by database cloning strategy and sequenced 2,907 bp from this cDNA which has a closed open reading frame (ORF) of 2,385 bp coding for a polypeptide of 795 amino acid residues. This cDNA, as its plant counterpart, encodes a bifunctional enzyme, with both gamma-glutamyl kinase (gamma-GK) and gamma-glutamyl phosphate reductase (gamma-GPR) activities that catalyzes the first 2 steps in proline biosynthesis and it hybridizes to a 4.5 kb mRNA from various tissues. A human genetic disease caused by a deficient P5CS has been recognized. The phenotypic features for deficiency of P5CS include joint hyperlaxity, skin hyperelasticity, cataract and mental retardation with hyperammonemia and low plasma levels of proline, citrulline and ornithine.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 3","pages":"171-8"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19733788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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