Clinical physiology and biochemistry最新文献

{"title":"NO-Synthase Pathway of L-Arginine Transformation in Spermatozoa of Infertile Men with Different Forms of Pathospermia","authors":"D. Vorobets, O. Оnufrovych, R. Fafula, U. Yefremova, Z. Vorobets","doi":"10.25040/ECPB2016.03.047","DOIUrl":"https://doi.org/10.25040/ECPB2016.03.047","url":null,"abstract":"Elucidation patterns of male germ cells functioning is of particular relevance in the present conditions due to increasing trends in male reproductive disorders [1]. Men infertility is caused by the effect of different pathological processes in genital organs and glands, resulting in pathospermia, which reduces fertilizing ability of sperm cells. Common methods for diagnosis of infertility including clinical and morphological, cytological, bacteriological methods do not always indicate the cause of the decrease in viability of spermatozoa and do not reveal mechanisms of the changes [13]. Over the past two decades a large amount of researches devoted to studying the role of nitric oxide and NO-synthase in the regu lation of physiological functions and pathological processes appeared. However, the role of NO-synthase in sperm cells in different forms of male infertility remains poorly understood [9, 11]. The NO formation in the human body is carried out as a result of oxidation of nitrogen atom that is part of the amino acid L-arginine by enzyme – Nitric Oxide Synthase (NOS) [4, 10, 11, 20]. There are three of its isoforms: neuronal – NOS (nNOS), inducible (macrophage) – NOS II (iNOS) and endothelial – NOS III (eNOS). Constitutive isoforms of NO-synthase (nNOS, eNOS) provide a synthesis of NO in physiological conditions. iNOS is inactive in physiological conditions and is activated in response to pathogenic stimuli [3–6, 12, 20, 26]. Therefore, the functional and regulatory features of inducible isoform of NOS depend on the nature of pathological process and the affected organ [12]. Both constitutive and inducible isoforms of NOS are related to NO production in the early phase of inflammation and their pro-inflammatory effect is manifested. Late phase of inflammation is associated with local leukocyte activity and infiltration. Its development is contributed only by NO, produced by iNOS, localized in leukocytes [12]. The elucidation of changes in NOS isoforms activity in men with impaired fertility determines actuality of present work. The aim of the research is to detect changes in the activity of NOS isoforms of sperm cells in patients with different forms of infertility. Materials and methods. Spermatozoa preparation. Semen samples of men aged 21–44 years were used in the research. Relatively healthy donors with no reproductive disorders and infertile men were among the men under study. The control group consisted of 20 healthy men with somatic fertility, normozoospermia and confirmed parenthood (married for 3–10 years and have 1–3 healthy children). Before turning to the study, all men had familiarized themselves with patient information leaflets and gave informed consent to participate in the research.","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"68 Supplement 1","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"2016-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75004933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monosynaptic Responce of Ventral Root of the Spinal Cord Cord in the Early Stages of Postdenervation Spinal Hyperreflexia","authors":"A. Rodinskiy, I. Gutnik, O. Klimenko, T. Demchenko, A. Petrenko, O. Kuzub","doi":"10.25040/ECPB2016.03.005","DOIUrl":"https://doi.org/10.25040/ECPB2016.03.005","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"8 1","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"2016-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87225665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Search for Imbalance Mechanisms of the Metabolic Indicators and Natural Detoxification at the Action of New 4-thiazolidinone Derivatives Possessing Anti-neoplastic Activity","authors":"L. Kobylinska, R. Lesyk, R. Stoyka, B. Zimenkovsky","doi":"10.25040/ECPB2016.03.024","DOIUrl":"https://doi.org/10.25040/ECPB2016.03.024","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"105 1","pages":"24-34"},"PeriodicalIF":0.0,"publicationDate":"2016-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79236391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Indices of Laboratory Animals Blood at Pre-Slaughter Stress","authors":"S. Grabovskyi, O. Grabovska, A. Velyka","doi":"10.25040/ECPB2016.02.034","DOIUrl":"https://doi.org/10.25040/ECPB2016.02.034","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"20 1","pages":"34-39"},"PeriodicalIF":0.0,"publicationDate":"2016-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73987794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Layering Aortic Aneurism. The Way to the Diagnosis","authors":"O. Trotsenko, N. Kyselchuk","doi":"10.25040/ECPB2016.02.084","DOIUrl":"https://doi.org/10.25040/ECPB2016.02.084","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"71 1","pages":"84-86"},"PeriodicalIF":0.0,"publicationDate":"2016-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84217091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ergonomic Aspects of Dentist’s Workplace. Part 1","authors":"O. Petryshyn, O. Kohut","doi":"10.25040/ECPB2016.02.058","DOIUrl":"https://doi.org/10.25040/ECPB2016.02.058","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"121 1","pages":"58-65"},"PeriodicalIF":0.0,"publicationDate":"2016-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79452569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Regulatory Systems Activity According to Analysis of the Heart Rate Variability of Rats under Introduction of Hydrogen Sulfide Donor NaHS","authors":"M. Gzhegotskyi, I. Kovalchuk, S. Kovalchuk","doi":"10.25040/ECPB2016.02.040","DOIUrl":"https://doi.org/10.25040/ECPB2016.02.040","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"34 1","pages":"40-44"},"PeriodicalIF":0.0,"publicationDate":"2016-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86474243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Syndrome – Modern overview of the Problem","authors":"O. Bondarenko, M. Sorochka","doi":"10.25040/ECPB2016.02.045","DOIUrl":"https://doi.org/10.25040/ECPB2016.02.045","url":null,"abstract":"During the last century the object of discussion between scholars has been the problem of integrated pathology. Published proceedings about this question between 1990–2000 were seldom, and from 2001 until 2010 their amount reached the point of 39 [22]. A condition which is characterized by the presence of several diseases is marked by an appellation “integrated” in academic literature, “multisystimetic” or “combined” pathology, “coexisted” or “associated” diseases and conditions [2–4]. Appellations “comorbidity” – for marking simultaneous affection of two organs, body systems or presence of two diseases; and “multimorbidity” – for marking more than 3 diseases at a time, are used more often in literature sources from English-speaking countries [20, 21]. Nowadays the problem of average increasing proportion of the total incidence of humanity with inveterate non-communicable diseases is being actively discussed. Metabolic syndrome (MS) is one of these problems that can cause many diseases and has already reached proportions of non-communicable epidemic, “epidemic of the 21 century.” Around one fourth of population in well developed countries appears to have MS [43]. We can definitely call it “civilization disease”. A huge part in its progression is made by urbanization, life style changes that lead to the lack of exercise, high-calorie food consumption and stress increasing. These factors continue constant increasing of arterial hypertension (AH), dislipidemy, adiposis and diabetes mellitus [1]. Patients with MS have 1.5–3 times higher risk of coronary heart disease (CHD) and apoplectic attack [5]. The average percentage of MS symptoms appear, such as disorders of cholesterol metabolism, glucose and high blood pressure, in the patients over 60 years old is 42–43,5 %. The death rate of CHD among these patients is 40% higher, of AH – 2,5–3 times higher, of type 2 diabetes – 4 times higher than among general population. Framingham Heart Study during which 5 thousand people from 18 to 74 years old had been carefully examined, gave a chance to identify – the combination of 3 or more components of MS leads to higher risk of CHD by 2.4 times more for males and 5,9 times more for females [6]. Core components of MS like abdominal obesity, hyperlipemia, insulin resistance (IR) are interdependent from the condition of the digestive system [14, 11, 16, 40]. Patients with MS have lower life quality than healthy men, that can be observed on the lower rate of physical and mental activity among both males and females by 1,5–2 times [7]. The survey which took place in Italy found out that the huge part of children (6–14 years old) has a risk factor for cardiometabolic","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"59 1","pages":"45-52"},"PeriodicalIF":0.0,"publicationDate":"2016-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80258554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CA-125 Antigen Concentration in Healthy Women of Different Age Groups and at Different Stages of the Ovary Malignant Neoplastic Transformation Development","authors":"O. Yakubets, D. Vorobets, L. Odnorih, Z. Vorobets, M. Gzhegotskyi","doi":"10.25040/ECPB2016.02.053","DOIUrl":"https://doi.org/10.25040/ECPB2016.02.053","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"41 1","pages":"53-58"},"PeriodicalIF":0.0,"publicationDate":"2016-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76301249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Amino Acids Derivatives of 1,4-Naphthоquinone on Membrane Related Processes During Loach Embryogenesis","authors":"A. Heneha, M. Bura","doi":"10.25040/ECPB2016.02.028","DOIUrl":"https://doi.org/10.25040/ECPB2016.02.028","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"22 1","pages":"28-33"},"PeriodicalIF":0.0,"publicationDate":"2016-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83241834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}