Clinical and Experimental Medicine最新文献

{"title":"Evaluating comparative effectiveness of pembrolizumab-based therapy versus chemotherapy in treatment of gastric carcinoma: a systematic review and meta-analysis of randomized controlled trials.","authors":"Haleema Mansoor, Maheen Gohar, Asma Attaria, Faiza Fatima Karim, Umaimah Naeem, Mohsin Khan, Javed Iqbal","doi":"10.1007/s10238-025-01610-5","DOIUrl":"10.1007/s10238-025-01610-5","url":null,"abstract":"<p><p>Gastric cancer, especially cancer of the gastro-esophageal junction, ranks among the first five cancers in the world with the highest mortality rates. It has poor survival rates for the advanced stages. Traditional chemotherapy, while standard, often results in significant side effects and limited efficacy. The objective of this meta-analysis and systemic review is to ascertain if pembrolizumab-based therapies for advanced gastric cancer are more effective and safer than standard chemotherapy. The focus consisted of RCTs with adults suffering from gastric carcinoma who received pembrolizumab every 3 weeks (200 mg) intra-related dose or with at least comparable chemotherapy regimen. Outcomes assessed are as follows: overall survival (OS), progression-free survival (PFS), and objective response rate (ORR). All potential sources regarding the search of outcome measures were applied: Google Scholar, Scopus, PubMed, and Cochrane library, and last search in June 2024 was carried out. Out of 568 articles screened, four RCTs comprising 2,831 patients met the inclusion criteria. Analysis indicated that pembrolizumab alone did not significantly improve OS compared to chemotherapy (HR 0.87). However, when combined with chemotherapy, pembrolizumab dramatically enhanced OS (HR 0.80) and PFS (HR 0.78). ORR was superior in the pembrolizumab plus chemotherapy group (RR 1.24), while pembrolizumab monotherapy showed no significant difference from chemotherapy alone. Safety analysis revealed a higher frequency of adverse events in the pembrolizumab-based therapy groups compared to chemotherapy. Pembrolizumab together with chemotherapy improves greater survival and higher levels of response rate in patients with severe gastric cancer, especially with high PD-L1 expression. But it has rather more adverse events, allowing patient monitoring with care.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"98"},"PeriodicalIF":3.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Darwinian approach to the development of the vascular system in the vertebrates.","authors":"Domenico Ribatti","doi":"10.1007/s10238-025-01633-y","DOIUrl":"10.1007/s10238-025-01633-y","url":null,"abstract":"<p><p>The vascular system originated around 600 million years ago. Endothelial cells evolved between 540 and 510 million years ago, and endothelial heterogeneity also developed. In invertebrates, two typologies have been described, the so-called open and closed systems, whereas in vertebrates only a closed system is present. In mammals, the presence of smooth muscle cells in the walls of small arteries regulates blood pressure and distribution to different organs; capillaries are involved in the exchange of gasses and metabolites; veins return the blood to the heart, whereas lymphatic vessels collect interstitial fluids and white blood cells and are in continuity with the venous system. Endothelial heterogeneity is the consequence of the different interactions of endothelium with the organ and tissue microenvironment including stromal cells, which is mediated by soluble factors or cell-cell/cell-extracellular matrix interactions leading to a particular phenotype of the endothelium. In this context, the heterogeneity of endothelial cells reflects specific responses to different microenvironments and their specialization to perform different functions, leading to different subsets of endothelial cells with unique gene expression patterns.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"97"},"PeriodicalIF":3.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium channels as pharmacological targets for cancer therapy.","authors":"Xiaozhen Liu, Changyun Feng, Li Yan, Jili Cao, Xinping Zhu, Mingqian Li, Guizhi Zhao","doi":"10.1007/s10238-025-01632-z","DOIUrl":"10.1007/s10238-025-01632-z","url":null,"abstract":"<p><p>Ca²⁺, as critical second messengers in biological processes, plays a pivotal role in the regulation of diverse cellular signaling pathways. The dysregulation of calcium signaling is intricately linked to the progression of various cancers. The capacity of Ca²⁺ to modulate cell death and proliferation, along with its potential for pharmacological manipulation, presents a promising avenue for the development of novel cancer therapeutics. This review provides a comprehensive overview of the classification of Ca²⁺ channels and their mechanisms of action in oncogenesis, explores the application of Ca²⁺ blockers in cancer treatment, and underscores the importance of conducting further clinical trials.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"94"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and functional dysregulations of CD8 + T Cells in myasthenia gravis.","authors":"Chang Liu, Hao Zhang, Yu-Yao Zhai, Jing Dong, Yang Zhou, Heng Li, Min Zhang, Chun-Lin Yang, Peng Zhang, Xiao-Li Li, Rui-Sheng Duan, Tong Du","doi":"10.1007/s10238-025-01603-4","DOIUrl":"10.1007/s10238-025-01603-4","url":null,"abstract":"<p><p>Myasthenia Gravis (MG) is a heterogeneous autoimmune disorder characterized by fluctuating muscle weakness caused by autoantibodies targeting neuromuscular junction components. While the role of CD4 + T cells in MG is well established, the contribution of CD8 + T cells remains poorly understood. In this study, we analyze CD8 + T cells in 36 MG patients and 38 age- and gender-matched controls using flow cytometry to evaluate subset distribution, granzyme expression, and cytokine production. MG patients exhibit an altered CD4 + /CD8 + T cell ratio and significant changes in CD8 + T cell subsets, including increased central memory CD8 + T cell (Tcm) proportions and decreased effector memory CD8 + T cell (Tem) proportions. Granzyme B expression in Tcm cells is significantly elevated in MG patients, whereas no significant changes are observed in other subsets or GZMK expression. Cytokine analysis reveals increased IL-21, GM-CSF, and IL-17A production by CD8 + T cells in MG patients. These phenotypic and functional alterations of CD8 + T cells persist during the acute phase of the disease, regardless of immunotherapy usage, and vary between ocular and generalized MG. Subgroup and correlation analyses further identify age-dependent and age-independent dysregulations of CD8 + T cells, indicating complex and subtype-specific roles of CD8 + T cells in the immunopathological processes underlying MG. Our findings provide novel insights into the involvement of CD8 + T cells in MG pathogenesis, laying a foundation for future research and potential therapeutic strategies targeting CD8 + T cells.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"96"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of clinical features between patients with and without renal involvement in IgG4-related disease.","authors":"Qixing Yu, Ganyuan He, Zhi Zhao, Jiayi Chen, Qiqi Huang, Wenke Hao, Xiangbin Mi, Wenxue Hu","doi":"10.1007/s10238-025-01612-3","DOIUrl":"10.1007/s10238-025-01612-3","url":null,"abstract":"<p><strong>Objective: </strong>The kidney is one of the organs most frequently affected in immunoglobulin G4-related disease (IgG4-RD). Early identification of IgG4-RD with renal injury by clinical features is a current challenge. There is a paucity of data regarding the clinical features of renal involvement in IgG4-RD.</p><p><strong>Materials: </strong>Patients with the diagnosis of IgG4-RD with and without renal injury were included in the retrospective cohort study. Cox regression analyses were used to investigate the risk factors for disease relapse and to construct the nomogram model.</p><p><strong>Results: </strong>From December 2014 to February 2022, 54 patients with IgG4-RD were retrospectively enrolled. Renal involvement in IgG4-RD was observed in 55.6% of the patients. The differences of age and lacrimal gland accumulation were statistically significant (P < 0.001, and P = 0.034, respectively). Age was significantly higher in the kidney injury group. Regarding laboratory findings, basophil counts, hemoglobin levels, and serum cholinesterase level were significantly lower in patients with renal involvement (P = 0.033, P = 0.006 and P = 0.019). Erythrocyte sedimentation rate level was significantly higher in patients with renal involvement (P = 0.017). Seven (23.4%) patients in the kidney injury group relapsed during follow-up with mean recurrence time 9.86 ± 7.08 months. Early diagnosis plays a key role in patient outcomes. Female, elevated erythrocyte sedimentation rate level, and elevated complement component 4 are the risk factors for the disease relapse of IgG4-RD patients. Moreover, an effective nomogram model has been developed to predict disease relapse in patients with IgG4-RD.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"95"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of leukemic stem cell (CD26 +) in chronic myeloid leukemia patients with different molecular responses and in treatment-free remission.","authors":"María Fernanda Camacho, Margot Peña, María Jazmín Toloza, Beatriz Moiraghi, Alicia Enrico, Romina Mariano, Florencia Negri, Carolina Pavlovsky, Verónica Ventriglia, María Josefina Freitas, Inés Engelberger, Raquel Bengió, Irene Larripa","doi":"10.1007/s10238-025-01626-x","DOIUrl":"10.1007/s10238-025-01626-x","url":null,"abstract":"<p><p>CD26 + leukemic stem cells (LSC) are a specific marker for chronic myeloid leukemia (CML), absent in healthy individuals and other myeloid neoplasms. These cells can contribute to disease resistance, as they are believed to sustain the leukemic clone despite effective tyrosine kinase inhibitor (TKI) therapy. This study analyzed CD26 + LSC and BCR::ABL1 transcript levels simultaneously using multiparametric flow cytometry and RT-qPCR in 210 chronic-phase patients undergoing TKI therapy and 31 patients in treatment-free remission (TFR). A significant decrease in LSC levels was observed as patients achieved deep molecular response (DMR, BCR::ABL1^IS ≤ 0.01%) (χ², p < 0.001). However, 19% (14/73) of DMR patients displayed persistent CD26 + LSC, suggesting a quiescent state without detectable BCR::ABL1 transcripts. A weak correlation (r = 0.187, p = 0.046) between LSC/µL absolute number and BCR::ABL1 transcript levels indicates a limited predictive value between these two variables. In TFR patients, LSC recurrence during follow-up did not correlate with molecular relapse, questioning their clinical relevance in this setting. In conclusion, while CD26 + LSC are frequently observed in patients with poor molecular response, their levels significantly decrease as patients achieve DMR. However, their persistence or recurrence in TFR lacks prognostic value for molecular relapse, indicating that CD26 + LSC are not reliable predictors of outcomes in CML.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"93"},"PeriodicalIF":3.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The necessity of adjuvant chemotherapy in young patients with T₁N₀M₀ breast cancer: a population-based study.","authors":"Sheng Chen, Shujie Chen, Wei Cao, Xiaoyun Zhou, Min Wei, Jie Wang, Li Yang","doi":"10.1007/s10238-025-01621-2","DOIUrl":"10.1007/s10238-025-01621-2","url":null,"abstract":"<p><p>Chemotherapy clearly adversely affects fertility in women of childbearing age. But it is not yet clear whether chemotherapy at the expense of fertility can benefit younger patients with early-stage breast cancer. We conducted a retrospective cohort study utilizing the Surveillance, Epidemiology, and End Results database and the Shanghai Jiao Tong University Breast Cancer Data Base spanning from 2010 to 2020 to investigate early-stage breast malignant carcinoma in patients aged between 20 and 39 years. To address covariate imbalance, propensity score matching (PSM) was employed with a ratio of 1:1 and caliper set at 0.02 standard deviation of propensity score. Univariate and multivariate analyses were performed to evaluate the impact of chemotherapy on both breast cancer-specific survival (BCSS) and overall survival (OS). We identified a total of 6265 patients with complete information about breast cancer. Among them, 3855 patients received chemotherapy. Following successful PSM, we obtained a matched cohort comprising 3038 patients where the characteristics between the two groups were balanced except for race. Kaplan-Meier survival analysis revealed no significant differences in BCSS (P = 0.183) and OS (P = 0.295) between the chemotherapy group and no-chemotherapy group. Similarly, in matched dataset. multivariate COX analysis revealed that chemotherapy did not significantly reduce the risk of BCSS (HR 1.332; 95% CI [0.865-2.051], P = 0.193) and OS (HR 1.225; 95% CI [0.818-1.833], P = 0.324). The chemotherapy group did not demonstrate a superior benefit in any of the subgroups when stratified analyses were conducted based on molecular subtype, tumor size, age, and ethnicity. Chemotherapy fails to significantly improve prognostic outcomes in young patients diagnosed with early-stage breast cancer. With the help of genetic testing, these patients can expect further step-down therapy in the future.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"92"},"PeriodicalIF":3.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prioritization of prognostic biomarkers regulated by calorie restriction in colon cancer through integrated biosignature analysis.","authors":"Oladayo E Apalowo, Joel J Komakech, Isaac D Boateng, Esther E Nwanna","doi":"10.1007/s10238-025-01630-1","DOIUrl":"10.1007/s10238-025-01630-1","url":null,"abstract":"<p><p>Colorectal cancer (CRC) remains a critical global health challenge, ranking second in cancer-related mortality and third in cancer incidence as of 2018, with risk increasing with age. Addressing its rising burden requires early diagnosis, prognostic biomarkers, and effective therapeutic strategies. Emerging evidence suggests that calorie restriction may mitigate aging-related functional decline and influence CRC progression, yet the molecular markers and mechanisms remain poorly understood. In this study, we analyzed the GSE24432 dataset, using multiple computational databases to screen differentially expressed genes (DEGs) associated with calorie restriction in CRC. Functional annotations, including Gene Ontology (GO), KEGG pathway analysis, and gene set enrichment analysis (GSEA), were undertaken to explore potential underlying mechanisms and pathways in CRC pathogenesis. Kaplan Meier and Cox proportional hazards regression analyses were conducted to establish the diagnostic and prognostic significance of the hub genes. The validation test was conducted via multiple databases. Our investigation identified 50 DEGs, using the cutoff criteria, p. adj < 0.05, |log2FC|> 0.3. GO and functional analysis results revealed extensive crosstalk of cellular and molecular components and pathways associated with mRNA and ribosome biogenesis, AMPK signaling, and p53 signaling pathway following calorie restriction. To understand how these DEGs drive biological reactions, we sorted the genes according to gene score > 3 and GO term > 3 and obtained 14 DEGs most relevant to the GO terms. Further analysis with GO CHORD showed that most genes are enriched in ribosome biogenesis and protein synthesis. Gene set enrichment analysis (GSEA) revealed the involvement of the hub genes in several hallmarks, such as tissue invasion and metastasis (p < 0.001), tumor-promoting inflammation (p < 0.001), resisting cell death (p < 0.01), and replicative immortality (p < 0.05). Survival analysis showed that higher expression of 7 hub genes, CDKN2A (p < 0.05), RPL9 (p < 0.02), TUBB6 (p < 0.01), and RPS15A (p < 0.01), and lower expression of CDKN1B (p < 0.01), NPM1 (p < 0.01), and RALA (p < 0.01), correlated to shorter survival of colon cancer. However, cross-reference of these genes revealed that calorie restriction decreased the expressions of CDKN2A and TUBB6 while CDKN1B and NPM1 were increased (p < 0.05). Several validation tests from multiple databases showed that high CDKN2A is associated with shorter overall survival rates, indicating CDKN2A is a therapeutic target and could serve as a more reliable biomarker for CRC prognosis. These findings could potentially facilitate the development of precision-based energy restriction interventions for CRC management, offering promising prospects for targeted therapeutic strategies for CRC patients.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"89"},"PeriodicalIF":3.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of 18F-FDG PET/CT in assessing systemic involvement in ANCA-associated vasculitis.","authors":"Song Yang, Haibo Tan","doi":"10.1007/s10238-024-01549-z","DOIUrl":"10.1007/s10238-024-01549-z","url":null,"abstract":"<p><p>The utilization of ¹⁸F-fluorodeoxyglucose positron emission tomography/computed tomography (¹⁸F-FDG PET/CT) has become a pivotal tool in diagnosing anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis, especially when the disease presents with neurological symptoms as the initial indicator. This advanced imaging technique was applied in a 68-year-old female patient who presented with recurrent limb weakness and intermittent blindness, symptoms that warranted thorough investigation due to their complexity and severity. The ¹⁸F-FDG PET/CT revealed significant radiotracer uptake in the kidneys, spleen, skeletal muscles, and right axillary lymph nodes, indicative of systemic involvement-a hallmark of ANCA-associated vasculitis (AAV) that can lead to multi-organ damage if not promptly managed. Complementary electromyography (EMG) identified multiple instances of peripheral nerve damage, adding further evidence to the diagnosis. This case underscores the intricate interplay between clinical symptoms, imaging findings, and laboratory results, all crucial in accurately diagnosing AAV. The findings highlight that ¹⁸F-FDG PET/CT not only facilitates early detection of neurogenic skeletal muscle damage and occult lesions, but also aids in precise disease classification, essential for guiding treatment strategies. The ability of this imaging modality to provide early warnings of major organ involvement offers clinicians a valuable opportunity to intervene before irreversible damage occurs, ultimately improving the accuracy of diagnosis and contributing to more effective management and outcomes for patients with this complex autoimmune disorder.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"90"},"PeriodicalIF":3.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GDF15 activates human fibroblast MRC5 cells via miR-338/STAT1 in silicosis.","authors":"Ge-Ting Wu, Qiu-Yan Tian, Bin Xie, Yong-Bin Hu, Zheng-Hao Deng","doi":"10.1007/s10238-025-01627-w","DOIUrl":"10.1007/s10238-025-01627-w","url":null,"abstract":"<p><p>Growth differentiation factor 15 (GDF-15) has been implicated in multiple biological functions. However, the role of GDF15 in silicosis remains unclear. In this study, the serum level of GDF-15 was investigated in 46 patients with silicosis by ELISA and results showed it was higher than that of control patients. The effects of exogenous GDF15 on mRNA and miRNA expression profiles of MRC5 cells were analyzed by RNA sequencing. GDF15 activated human embryonic lung fibroblast MRC5 cells with upregulation of col1a and α-SMA. GDF15 reduced miR-338 expression and increased STAT1 expression in MRC5 cells. The results of the luciferase reporter assay and bioinformatics analysis indicated that STAT1 was a direct target of miR-338. miR-338 mimics down-regulated col1a and α-SMA expression induced by GDF15 with STAT1 overexpression, whereas miR-338 inhibitor up-regulated col1a and α-SMA expression induced by GDF15 with STAT1 knockdown. Those results indicated GDF15 activated MRC5 cells through the miR-338/STAT1 pathway and GDF-15 may play an important role in silicosis.</p>","PeriodicalId":10337,"journal":{"name":"Clinical and Experimental Medicine","volume":"25 1","pages":"91"},"PeriodicalIF":3.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}