Journal La Lifesci最新文献

Optimization of PCR Analysis Based on Start Codon Targeted Markers (SCoT Markers) for Identification of Genetic Variation of Seaweed from Central Sulawesi 基于起始密码子定向标记（SCoT 标记）的 PCR 分析优化，用于鉴定中苏拉威西岛海藻的遗传变异

Journal La Lifesci Pub Date : 2024-02-12 DOI: 10.37899/journallalifesci.v5i1.1062

Tri Joko Santoso, Ali Husni, Kristianto Nugroho, Zakirah Raihani Ya’la, Triyani Dewi, Marhawati Marhawati, Maemunah Maemunah, Eka Rosyida, S. Ndobe

{"title":"Optimization of PCR Analysis Based on Start Codon Targeted Markers (SCoT Markers) for Identification of Genetic Variation of Seaweed from Central Sulawesi","authors":"Tri Joko Santoso, Ali Husni, Kristianto Nugroho, Zakirah Raihani Ya’la, Triyani Dewi, Marhawati Marhawati, Maemunah Maemunah, Eka Rosyida, S. Ndobe","doi":"10.37899/journallalifesci.v5i1.1062","DOIUrl":"https://doi.org/10.37899/journallalifesci.v5i1.1062","url":null,"abstract":"Seaweed is a fishery commodity that has high economic value because it contains carrageenan which can be used in the pharmaceutical, food, cosmetics, and industrial fields. Central Sulawesi Province is the second largest seaweed producer in Indonesia after South Sulawesi Province. The genetic diversity of seaweed in Central Sulawesi has not been studied much. Therefore, molecular-based characterization activities to identify the genetic variation of seaweed in the province are urgently needed. The purpose of the study was to optimize PCR techniques based on targeted start codon markers (SCoT markers) to identify the genetic diversity of seaweed accessions from Central Sulawesi Province. The results showed that the parameters for seaweed PCR amplification have been optimized. Of the fifteen SCoT primers, 10 of them can work to amplify seaweed DNA collected from several regions in Central Sulawesi. This is shown by the production of PCR result amplicons. PCR results also indicate polymorphism from the seaweed samples tested, although some seaweed samples have not been successfully amplified. The parameters of the PCR technique still need to be improved, especially the concentration of DNA prints, so that valid PCR results will be obtained for use in identifying seaweed genetic diversity.","PeriodicalId":102565,"journal":{"name":"Journal La Lifesci","volume":"74 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139843790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of PCR Analysis Based on Start Codon Targeted Markers (SCoT Markers) for Identification of Genetic Variation of Seaweed from Central Sulawesi 基于起始密码子定向标记（SCoT 标记）的 PCR 分析优化，用于鉴定中苏拉威西岛海藻的遗传变异

Journal La Lifesci Pub Date : 2024-02-12 DOI: 10.37899/journallalifesci.v5i1.1062

Tri Joko Santoso, Ali Husni, Kristianto Nugroho, Zakirah Raihani Ya’la, Triyani Dewi, Marhawati Marhawati, Maemunah Maemunah, Eka Rosyida, S. Ndobe

{"title":"Optimization of PCR Analysis Based on Start Codon Targeted Markers (SCoT Markers) for Identification of Genetic Variation of Seaweed from Central Sulawesi","authors":"Tri Joko Santoso, Ali Husni, Kristianto Nugroho, Zakirah Raihani Ya’la, Triyani Dewi, Marhawati Marhawati, Maemunah Maemunah, Eka Rosyida, S. Ndobe","doi":"10.37899/journallalifesci.v5i1.1062","DOIUrl":"https://doi.org/10.37899/journallalifesci.v5i1.1062","url":null,"abstract":"Seaweed is a fishery commodity that has high economic value because it contains carrageenan which can be used in the pharmaceutical, food, cosmetics, and industrial fields. Central Sulawesi Province is the second largest seaweed producer in Indonesia after South Sulawesi Province. The genetic diversity of seaweed in Central Sulawesi has not been studied much. Therefore, molecular-based characterization activities to identify the genetic variation of seaweed in the province are urgently needed. The purpose of the study was to optimize PCR techniques based on targeted start codon markers (SCoT markers) to identify the genetic diversity of seaweed accessions from Central Sulawesi Province. The results showed that the parameters for seaweed PCR amplification have been optimized. Of the fifteen SCoT primers, 10 of them can work to amplify seaweed DNA collected from several regions in Central Sulawesi. This is shown by the production of PCR result amplicons. PCR results also indicate polymorphism from the seaweed samples tested, although some seaweed samples have not been successfully amplified. The parameters of the PCR technique still need to be improved, especially the concentration of DNA prints, so that valid PCR results will be obtained for use in identifying seaweed genetic diversity.","PeriodicalId":102565,"journal":{"name":"Journal La Lifesci","volume":"43 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139783964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of Coral Reefs in the Tourism Zone of Hoga Island, Wakatobi National Park, Southeast Sulawesi, Indonesia 印度尼西亚东南苏拉威西瓦卡托比国家公园霍加岛旅游区珊瑚礁研究

Journal La Lifesci Pub Date : 2024-02-02 DOI: 10.37899/journallalifesci.v5i1.905

Hawati Hawati, Mustafa Mustafa, Yusuf Yusuf, Angkasa Putra, Herianto Suriadin

引用次数: 0

Study of Coral Reefs in the Tourism Zone of Hoga Island, Wakatobi National Park, Southeast Sulawesi, Indonesia 印度尼西亚东南苏拉威西瓦卡托比国家公园霍加岛旅游区珊瑚礁研究

Journal La Lifesci Pub Date : 2024-02-02 DOI: 10.37899/journallalifesci.v5i1.905

Hawati Hawati, Mustafa Mustafa, Yusuf Yusuf, Angkasa Putra, Herianto Suriadin

引用次数: 0

Oil Issues of the Smallest Arabic Member of the OPEC 欧佩克最小阿拉伯成员国的石油问题

Journal La Lifesci Pub Date : 2024-02-01 DOI: 10.37899/journallalifesci.v5i1.883

E. Khartukov

引用次数: 0

The The Effect of Types Planting Media and the Growth Regulatory Substances of Sprouts Extract on the Growth and Yield of Long Bean (Vigna unguiculata L.) 不同类型的种植介质和芽菜提取物中的生长调节物质对长豆角（Vigna unguiculata L.）生长和产量的影响

Journal La Lifesci Pub Date : 2024-02-01 DOI: 10.37899/journallalifesci.v5i1.807

Choirul Lisa Ramadhanti, Setiyono Setiyono, Gatot Subroto, Ika Purnamasari, Susan Barbara Patricia SM, A. P. Arum, Dyah Ayu Savitri

{"title":"The The Effect of Types Planting Media and the Growth Regulatory Substances of Sprouts Extract on the Growth and Yield of Long Bean (Vigna unguiculata L.)","authors":"Choirul Lisa Ramadhanti, Setiyono Setiyono, Gatot Subroto, Ika Purnamasari, Susan Barbara Patricia SM, A. P. Arum, Dyah Ayu Savitri","doi":"10.37899/journallalifesci.v5i1.807","DOIUrl":"https://doi.org/10.37899/journallalifesci.v5i1.807","url":null,"abstract":"Long bean production in East Java has declined in the last three years because of the decreased soil fertility and the failure of pod formation. The addition of organic matter to the soil planting medium and spraying ZPT bean sprout extract are some efforts to increase the supply of nutrients and overcome flower fall. This study aimed to determine the interaction between the treatment of the planting media and ZPT of bean sprout extract toward growth and yield of long bean plants. The study was conducted factorially using a completely randomized design (CRD) in 3 replications. The first factor is the type of planting medium namely M0: soil; M1: soil+ compost; M2: soil+ chaff charcoal; M3: soil+ cocopeat. The second factor was the concentration of ZPT bean sprout extract, namely T0 control; T1: 10%; T2: 20%; T3: 30%. There was a very significant interaction between the treatment of the planting media and the bean sprout extract ZPT on the variable number of pods per plant and wet weight of pods per plant. The best treatment was the combination of the type of soil and compost growing media with ZPT bean sprout extract 20%. The main effect of the type of planting media treatment showed that the results were not significantly different for all observed variables. The ZPT treatment of bean sprout extract significantly affected the variables of flowering age, harvest age, and pod length per plant, the best treatment was the concentration of ZPT bean sprout extract 20%.","PeriodicalId":102565,"journal":{"name":"Journal La Lifesci","volume":"20 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139815492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oil Issues of the Smallest Arabic Member of the OPEC 欧佩克最小阿拉伯成员国的石油问题

Journal La Lifesci Pub Date : 2024-02-01 DOI: 10.37899/journallalifesci.v5i1.883

E. Khartukov

引用次数: 0

The The Effect of Types Planting Media and the Growth Regulatory Substances of Sprouts Extract on the Growth and Yield of Long Bean (Vigna unguiculata L.) 不同类型的种植介质和芽菜提取物中的生长调节物质对长豆角（Vigna unguiculata L.）生长和产量的影响

Journal La Lifesci Pub Date : 2024-02-01 DOI: 10.37899/journallalifesci.v5i1.807

Choirul Lisa Ramadhanti, Setiyono Setiyono, Gatot Subroto, Ika Purnamasari, Susan Barbara Patricia SM, A. P. Arum, Dyah Ayu Savitri

{"title":"The The Effect of Types Planting Media and the Growth Regulatory Substances of Sprouts Extract on the Growth and Yield of Long Bean (Vigna unguiculata L.)","authors":"Choirul Lisa Ramadhanti, Setiyono Setiyono, Gatot Subroto, Ika Purnamasari, Susan Barbara Patricia SM, A. P. Arum, Dyah Ayu Savitri","doi":"10.37899/journallalifesci.v5i1.807","DOIUrl":"https://doi.org/10.37899/journallalifesci.v5i1.807","url":null,"abstract":"Long bean production in East Java has declined in the last three years because of the decreased soil fertility and the failure of pod formation. The addition of organic matter to the soil planting medium and spraying ZPT bean sprout extract are some efforts to increase the supply of nutrients and overcome flower fall. This study aimed to determine the interaction between the treatment of the planting media and ZPT of bean sprout extract toward growth and yield of long bean plants. The study was conducted factorially using a completely randomized design (CRD) in 3 replications. The first factor is the type of planting medium namely M0: soil; M1: soil+ compost; M2: soil+ chaff charcoal; M3: soil+ cocopeat. The second factor was the concentration of ZPT bean sprout extract, namely T0 control; T1: 10%; T2: 20%; T3: 30%. There was a very significant interaction between the treatment of the planting media and the bean sprout extract ZPT on the variable number of pods per plant and wet weight of pods per plant. The best treatment was the combination of the type of soil and compost growing media with ZPT bean sprout extract 20%. The main effect of the type of planting media treatment showed that the results were not significantly different for all observed variables. The ZPT treatment of bean sprout extract significantly affected the variables of flowering age, harvest age, and pod length per plant, the best treatment was the concentration of ZPT bean sprout extract 20%.","PeriodicalId":102565,"journal":{"name":"Journal La Lifesci","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139875216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digitalization of Marketing of Laskar Pelangi Local Agricultural Products Laskar Pelangi 本地农产品营销数字化

Journal La Lifesci Pub Date : 2024-01-02 DOI: 10.37899/journallalifesci.v4i6.971

Hardianti Hardianti, Nurmayanti Nurmayanti, Sandi Lubis, Sundari Sundari, Zulkifli N, Reni Angareani

引用次数: 0

Analysis of Biogas Assistance Program in Dairy Farm Development 奶牛场发展中的沼气援助计划分析

Journal La Lifesci Pub Date : 2024-01-02 DOI: 10.37899/journallalifesci.v4i6.988

Dila Ananda Syabillah, Mochamad Ali Mauldin, Marina Sulistyati

{"title":"Analysis of Biogas Assistance Program in Dairy Farm Development","authors":"Dila Ananda Syabillah, Mochamad Ali Mauldin, Marina Sulistyati","doi":"10.37899/journallalifesci.v4i6.988","DOIUrl":"https://doi.org/10.37899/journallalifesci.v4i6.988","url":null,"abstract":"Corporate Social Responsibility (CSR) is a form of corporate social responsibility towards the community and environment around the company. The biogas assistance program is one of PJT II's CSR programs in order to accelerate the handling of damage to the Citarum river watershed (DAS) and for the purpose of empowering the community, especially dairy farmers who come from members of the KPBS Pangalengan dairy farmer group. The aim of this research is to determine the mechanism for implementing the biogas assistance program as part of CSR activities and to analyze the benefits of implementing the biogas assistance program in developing the sustainability of dairy farming businesses. This research was conducted in November – December 2023. This research was conducted using a qualitative approach using the case study method. Primary data was obtained through in-depth interviews with informants selected purposively from twenty-one people consisting of the company, participants receiving assistance and implementing parties. The results of the research show that the implementation of the biogas assistance program has been implemented through a process planning, organizing, actuating, controlling (POAC) in the program mechanism, apart from that, the analysis of the program's objectives is to reduce pollution and damage to the Citarum watershed as a result of breeder activities and provide opportunities to increase income independently and sustainably and is an effort to increase breeder awareness to better protect the surrounding environment so that it can speed up the handling of damage to river watersheds that is currently occurring.","PeriodicalId":102565,"journal":{"name":"Journal La Lifesci","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0