Chronicles of Young Scientists最新文献_第8页

Periodontal status in HIV-positive individuals and its possible correlation with CD4+T cell count hiv阳性个体的牙周状况及其与CD4+T细胞计数的可能相关性

Chronicles of Young Scientists Pub Date : 2012-04-01 DOI: 10.4103/2229-5186.98689

K. Asif, K. Neelima, Shaila V Kothiwale, R. Patil

{"title":"Periodontal status in HIV-positive individuals and its possible correlation with CD4+T cell count","authors":"K. Asif, K. Neelima, Shaila V Kothiwale, R. Patil","doi":"10.4103/2229-5186.98689","DOIUrl":"https://doi.org/10.4103/2229-5186.98689","url":null,"abstract":"Background : Infection with human immunodeficiency virus (HIV) results in loss of immunologic functions, especially those coordinated by CD4+ T-helper cells and consequent impairment of immune response. Periodontal disease has been associated with HIV infection, and HIV infection has been considered a modifier of periodontal disease. Aim: The aim of this study was to report the severity of periodontal disease in HIV-positive individuals and its association between clinical periodontal indices and CD4+T-cell count. Materials and Methods: 25 HIV-positive individuals were recruited and medical history was recorded. To evaluate periodontal disease, clinical attachment loss (CAL), oral hygiene index (OHI), and gingival bleeding index (GI) were recorded. Immune suppression was measured by peripheral blood CD4+T cells/mm 3 as analyzed by flow cytometry. Statistical Analysis: Association between CD4+ T levels and clinical parameters were determined using correlation coefficient test. Results: When all subjects were evaluated, a negative correlation was obtained between CD4+ T-cell count and clinical attachment loss ( r = -0.68226). In individuals with CD4+cell counts 3 , a negative correlation was obtained between clinical attachment loss (-0.35467) and GI (-0.35202). In patients with CD4 count Conclusion: Immune suppression in combination with risk factors may increase progression of periodontal disease. Hence, these individuals should practice better oral hygiene and regular follow-up.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84281841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Studies on inclusion complex as potential systems for enhancement of oral bioavailability of olmesartan medoxomil 包合物作为提高奥美沙坦-美多索米口服生物利用度的潜在体系的研究

Chronicles of Young Scientists Pub Date : 2012-04-01 DOI: 10.4103/2229-5186.98685

Hetal Thakkar, B. V. Patel, M. Parmar, Nirav P Chauhan, Arpita A Patel

{"title":"Studies on inclusion complex as potential systems for enhancement of oral bioavailability of olmesartan medoxomil","authors":"Hetal Thakkar, B. V. Patel, M. Parmar, Nirav P Chauhan, Arpita A Patel","doi":"10.4103/2229-5186.98685","DOIUrl":"https://doi.org/10.4103/2229-5186.98685","url":null,"abstract":"Background: Olmesartan medoxomil (OLM), an anti-hypertensive agent administered orally, has absolute bioavailability of only 26% due to the poor aqueous solubility (7.75 μg/ml). Inclusion complexation with cyclodextrins (CD) has been reported to increase the aqueous solubility of various compounds. Aim: The present investigation aimed to enhancing the oral bioavailability of OLM by inclusion complexation with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD). Materials and Methods: The inclusion complexes with HP-β-CD were prepared using two different methods, viz., physical mixture and kneading. The prepared complexes were characterized for various parameters such as drug content, aqueous solubility, dissolution study, in vitro diffusion, intestinal permeability and stability study. The formation of the inclusion complex was confirmed by differential scanning calorimetry, X-ray diffraction, and Fourier transform infrared spectroscopy. Results: The solubility, dissolution, diffusion rate, and intestinal permeability of the prepared complexes were found to be significantly higher than that of the plain drug. Among the two methods used for formation of inclusion complex, KN method gave higher solubility rates and hence is a better method when compared with PM. Conclusion: The approach seems to be promising in improving the oral bioavailability of OLM.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84661406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Formulation and characterization of calcium chloride guar gum microsphere of theophylline 茶碱氯化钙瓜尔胶微球的制备及表征

Chronicles of Young Scientists Pub Date : 2012-04-01 DOI: 10.4103/2229-5186.98686

S. Verma, D. Jain, S. Shukla

引用次数: 3

Characterization of fungal biofilm-based catheter-related sepsis 基于真菌生物膜的导管相关败血症的特征

Chronicles of Young Scientists Pub Date : 2012-01-01 DOI: 10.4103/2229-5186.94317

M. Singhai, A. Malik, M. Shahid, M. A. Malik, R. Goyal

{"title":"Characterization of fungal biofilm-based catheter-related sepsis","authors":"M. Singhai, A. Malik, M. Shahid, M. A. Malik, R. Goyal","doi":"10.4103/2229-5186.94317","DOIUrl":"https://doi.org/10.4103/2229-5186.94317","url":null,"abstract":"Background: Fungi most commonly associated with nosocomial septicemia are in the genus Candida . Attachment of yeasts to intravascular surfaces is the crucial step in initiating colonization by yeast cells, forming biofilms and resulting in disseminated infection depending on various factors. Aims: To study the rate and profile of fungal biofilms in catheter-related sepsis (CRS) and antifungal resistance among the clinical isolates of CRS was the aim of this study. Materials & Methods: In all, 135 hospitalized pediatric patients with peripheral intravascular catheters (IVCs) and clinical suspicion of nosocomial septicemia were studied. The yeast isolates causing CRS were identified and characterized, and antifungal susceptibility testing by microplate alamar blue method (minimum inhibitory concentration) was also done. The fungal biofilm formations were visualized by scanning electron microscopy and tube method. Results: 7.4% patients with IVC had CRS, majority being caused by Candida albicans biofilms. In vitro antifungal susceptibility testing of yeast isolates causing CRS demonstrated moderate to high level of resistance to fluconazole (70%). Voriconazole was the most optimum drug to cure such infections. Conclusion: This study illustrates the need for exploration of biofilm-based CRS (fungemia) in hospitalized patients and to design practical guidelines for their management (diagnosis and treatment).","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85570861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

LC-MS metabolomic analysis of environmental stressor impacts on the metabolite diversity in Nephthea spp. 环境胁迫对肾草代谢物多样性影响的LC-MS代谢组学分析。

Chronicles of Young Scientists Pub Date : 2012-01-01 DOI: 10.4103/2229-5186.94319

H. Januar, E. Marraskuranto, G. Patantis, E. Chasanah

{"title":"LC-MS metabolomic analysis of environmental stressor impacts on the metabolite diversity in Nephthea spp.","authors":"H. Januar, E. Marraskuranto, G. Patantis, E. Chasanah","doi":"10.4103/2229-5186.94319","DOIUrl":"https://doi.org/10.4103/2229-5186.94319","url":null,"abstract":"Context: The soft coral Nephthea spp. is a source of terpenoid class that potentially has pharmaceutical properties. However, metabolite diversity and cytotoxic activity of this species are varied among coral reefs from various sites. Aim: To analyze the water quality in Nephthea spp. environment as a possible factor causing a difference in its metabolite diversity. Settings and Design: Nephthea spp. from seven sites were taken in October 2010 at the Alor District of Marine Protected Area, Indonesia. Materials and Methods: Water quality assessment was analyzed in situ and indexed by Canadian Council of Ministry Environment-Water Quality Index (CCME-WQI) method. Meanwhile, metabolite diversity was analyzed by a LC-MS metabolomic method, using C18 reversed phase and gradient water-acetonitrile system. Statistical Analysis Used: Spearman's rho and regression analysis were applied to correlate the water quality index to ecological index (richness, diversity, and evenness) from LC-MS results. Results: The water quality index had a significant positive correlation and strong linear regression determinant to the total metabolite (R 2 = 0.704), particularly to semipolar metabolite richness (R 2 = 0.809), the area of terpenoid class in the organism. Conclusion: It can be concluded that water quality may serve as a major factor that affects the amount of richness in Nephthea spp. metabolites. When the water quality is lower, as environment stresses increases, it may affect the metabolite richness within direct disrupt of metabolite biosynthesis or indirect ecological means. Terpenoids are known as a soft coral antipredator (coral fishes), the amount of which depends on the water quality.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88508811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Method development and validation: Skills and tricks 方法开发和验证:技巧和技巧

Chronicles of Young Scientists Pub Date : 2012-01-01 DOI: 10.4103/2229-5186.94303

Ashok Kumar, Lalit Kishore, Navpreet Kaur, Anroop B Nair

引用次数: 18

Simultaneous estimation of captopril and hydrochlorothiazide in combined dosage forms 卡托普利与氢氯噻嗪联合剂型的同时评价

Chronicles of Young Scientists Pub Date : 2012-01-01 DOI: 10.4103/2229-5186.94312

K. S. Rao, M. Panda, N. K. Keshar, S. Yadav

{"title":"Simultaneous estimation of captopril and hydrochlorothiazide in combined dosage forms","authors":"K. S. Rao, M. Panda, N. K. Keshar, S. Yadav","doi":"10.4103/2229-5186.94312","DOIUrl":"https://doi.org/10.4103/2229-5186.94312","url":null,"abstract":"Aim: This work deals with the simultaneous determination of captopril (CAP) and hydrochlorothiazide (HZ) in two-component solid dosage form, without prior separation, by three different techniques (simultaneous equation, absorbance ratio method, and first-order derivative method). Materials and Methods: This work was carried out on Shimadzu electron UV1800 double-beam UV-Visible spectrophotometer. The absorption spectra of reference and test solutions were carried out in 1 cm matched quartz cell over the range of 200-400 nm. Methanol and distilled water are used as solvent. Results: The first method is the application of simultaneous equation. Where the linearity ranges for both the drugs were 5-35 μg/ml. The second method is the determination of ratio of absorbance at 271 nm, the maximum absorption of HZ and isobestic wavelength 209 nm, the linearity ranges for both the drugs were 10-120 μg/ml The third method is the first-order derivative method, where the CAP shows wavelength at 222 nm and HZ shows at 340 nm, and the linearity ranges for CAP and HZ were 1-30 μg/ml and 1-40 μg/ml, respectively. The proposed procedures were successfully applied for the simultaneous determination of both the drugs in commercial tablet preparation. The validity of the proposed methods was assessed by applying the standard addition technique where the percentage recovery of the added standard was found to be 99.52±0.214 and 99.00±0.165 using the simultaneous equation method, 99.76±0.684 and 99.58±0.279 using the graphical absorbance ratio method, and 99.45±0295 and 99.21±0.678 using first derivative method, for CAP and HZ, respectively. Conclusion: The proposed procedures are rapid, simple, require no preliminary separation steps, and can be used for routine analysis of both drugs in quality control laboratories.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82914271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Desmoplastic small-round-cell tumor presenting as a Sister Joseph nodule 结缔组织增生小圆细胞肿瘤，表现为约瑟夫姐妹结节

Chronicles of Young Scientists Pub Date : 2012-01-01 DOI: 10.4103/2229-5186.94306

A. Rekha, Shalinee Rao, J. Thanka, S. Rajendiran, A. Ravi

引用次数: 0

Bio-inspired nanomaterials and their applications as antimicrobial agents 仿生纳米材料及其作为抗菌剂的应用

Chronicles of Young Scientists Pub Date : 2012-01-01 DOI: 10.4103/2229-5186.94314

S. Zinjarde

引用次数: 60

Multifunctional role of green blood therapy to cure for many diseases 绿色血液治疗多种疾病的多功能作用

Chronicles of Young Scientists Pub Date : 2012-01-01 DOI: 10.4103/2229-5186.94305

V. Singhal, A. Singhal, K. Padmavathi, Riya Sil, Seth G. L. Bihani

{"title":"Multifunctional role of green blood therapy to cure for many diseases","authors":"V. Singhal, A. Singhal, K. Padmavathi, Riya Sil, Seth G. L. Bihani","doi":"10.4103/2229-5186.94305","DOIUrl":"https://doi.org/10.4103/2229-5186.94305","url":null,"abstract":"Juice of wheat (Triticum aestivum L., Poaceae) grass is termed as green blood. Wheatgrass is a variety of grass that is used like a herbal medicine for its therapeutic and nutritional properties. The aim of this study is to concise the health benefits of green blood therapy. As wheatgrass juice (WGJ) bears a close resemblance to the “hemoglobin” in our blood, the juice is called as “green blood” and the therapy using it is called as “green blood therapy”. The WGJ is a complete food and contains carbohydrates, proteins, all essential minerals, and vitamins. Wheatgrass therapy is recommended for patients suffering from chronic diseases such as asthma, atherosclerosis, Parkinson’s disease, joint pains, TB, constipation, hypertension, diabetes, bronchitis, insomnia, eczema, sterility, hemorrhage, obesity, and flatulence. It is also useful in the treatment of cancer. The WGJ is immediately absorbed into the bloodstream and gives energy. On an empty stomach, it is assimilated into blood in about 20 min. The energy lasts throughout the day. It is practically a fountain of youth and found to have numerous health benefits. This review is concluded that the WGJ has higher degree of curative index and this can be last alternative therapy when the all therapy will fail.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73562669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8