Clinical biochemistry最新文献

{"title":"Silent electrolyte imbalance unmasked by paralysis: a case of hypokalemia in a middle-aged woman","authors":"Aman Advani ,&nbsp;Hassan Ahmed ,&nbsp;Muhammad Ahsan Shabbir ,&nbsp;Maaz Ahmed ,&nbsp;Tauqeer Khan","doi":"10.1016/j.clinbiochem.2026.111088","DOIUrl":"10.1016/j.clinbiochem.2026.111088","url":null,"abstract":"<div><h3>Background</h3><div>Hypokalemic periodic paralysis is a rare autosomal dominant muscle channelopathy often misdiagnosed due to its atypical presentation. It is characterized by episodic flaccid paralysis with low serum potassium levels and may be triggered by factors such as high carbohydrate intake, infections, or medications.</div></div><div><h3>Case presentation</h3><div>We report the case of a 25-year-old female who presented with acute ascending flaccid paralysis and was initially misdiagnosed with Guillain-Barré syndrome. Despite biochemical and electrocardiographic evidence of hypokalemia (serum K⁺: 2.7 mEq/L; electrocardiogram showing U waves and flattened T waves), potassium correction was not initiated. The patient rapidly deteriorated, required mechanical ventilation, and ultimately succumbed to her condition. Thyroid function testing was not performed despite clinical red flags, including a history of anxiety and depression, raising suspicion for thyrotoxic periodic paralysis.</div></div><div><h3>Discussion</h3><div>This case highlights the diagnostic challenges of hypokalemic paralysis, particularly when presenting as a neurological emergency without classic features. The failure to promptly correct hypokalemia and investigate its underlying etiology contributed to a preventable fatal outcome. A systematic “hypokalemia-first” approach is recommended to avoid such diagnostic delays in similar clinical settings.</div></div><div><h3>Conclusion</h3><div>Timely recognition and correction of hypokalemia, alongside targeted evaluation of underlying causes such as thyrotoxicosis, are critical in patients presenting with acute flaccid paralysis. Standardized emergency protocols could significantly improve outcomes and prevent avoidable fatalities.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"142 ","pages":"Article 111088"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TUG1 and miR-145 as potential biomarkers associating hypothyroidism to cardiovascular risk","authors":"Tarek K. Motawi ,&nbsp;Olfat G. Shaker ,&nbsp;Haidy Saied ,&nbsp;Noha H. Sayed","doi":"10.1016/j.clinbiochem.2026.111104","DOIUrl":"10.1016/j.clinbiochem.2026.111104","url":null,"abstract":"<div><h3>Objectives</h3><div>RNA based epigenetic modifications and their relations with chronic diseases have been widely studied. The current study aimed to determine the role of long noncoding RNA (lncRNA) TUG1 and microRNA 145 (miR-145) in the pathophysiology and progression of hypothyroidism as well as their impact on subsequent cardiovascular disorders.</div></div><div><h3>Design &amp; Methods</h3><div>Serum levels of TUG1 and miR-145 were measured in three different groups, 40 overt hypothyroid (OHT) patients, 40 subclinical hypothyroid (SHT) patients and 45 healthy controls. All individuals were subjected to a full history investigation and clinical examination. Flow mediated vasodilation of the brachial artery was measured to evaluate endothelial function. Serum samples were also tested for T4, TSH, TG, TC, HDL, LDL, HbA1c, glucose and insulin.</div></div><div><h3>Results</h3><div>TUG1 level was significantly increased in OHT and SHT patients compared to the healthy controls. TUG1 correlated positively with TC, LDL levels and endothelial dysfunction, while correlated negatively with HDL. Regarding miR-145, its expression level was downregulated only in OHT patients as compared to controls and SHT patients. There were significant negative correlations with TC, LDL and endothelial dysfunction and significant positive correlation with HDL. A significant negative correlation existed between TUG1 and miR-145.</div></div><div><h3>Conclusions</h3><div>TUG1 could be considered a powerful diagnostic tool to distinguish HT patients, while miR-145 could serve as a negative independent predictor for the progression of HT among subclinical patients. TUG1/miR-145 trajectory could affect the progression of hypothyroidism and the incidence of cardiovascular diseases through impacting patients’ lipid profile and endothelial integrity. They could be potential biomarkers and predictors for hypothyroidism pathophysiology.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"142 ","pages":"Article 111104"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146776059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unresolved issues of multiple citrulline similar-motif antigen in diagnosing rheumatoid arthritis: from specificity to clinical practice","authors":"Nayan Chen","doi":"10.1016/j.clinbiochem.2026.111083","DOIUrl":"10.1016/j.clinbiochem.2026.111083","url":null,"abstract":"","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"142 ","pages":"Article 111083"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of three NT-proBNP assays for heart failure","authors":"Isaiah K. Mensah ,&nbsp;Brittany Roemmich ,&nbsp;Sydny Lawless ,&nbsp;Alexis Wysocki ,&nbsp;David Daghfal ,&nbsp;Christian J. Hunter ,&nbsp;Christopher W. Farnsworth","doi":"10.1016/j.clinbiochem.2025.111062","DOIUrl":"10.1016/j.clinbiochem.2025.111062","url":null,"abstract":"<div><h3>Background</h3><div>N-terminal pro-B-type natriuretic peptide (NT-proBNP) measurement is recommended by guidelines for diagnosing and managing heart failure (HF). Accurate NT-proBNP measurement is critical for timely and effective treatment, but limited studies have compared NT-proBNP results from multiple platforms, and few studies have compared their concordance using commonly used clinical cutpoints.</div></div><div><h3>Methods</h3><div>The Alinity, PathFast, and Roche NT-proBNP assays were assessed in 276 patient samples, comprising 188 inpatients and 88 outpatients with normal renal function. Correlation and bias among the assays were assessed, and guideline-endorsed clinical cutpoints were used to evaluate concordance.</div></div><div><h3>Results</h3><div>Passing-Bablok regression revealed a slope of 1.00 (95% CI: 0.99–1.01) for Alinity vs. Roche, 1.14 (1.11–1.16) for PathFast vs. Roche, and 1.11 (1.01–1.13) for PathFast vs. Alinity. Bland-Altman analysis revealed minimal bias among the assays, with the closest agreement observed between PathFast and Alinity (−1.85%, −43.36 to 39.65). On inpatients, the concordance was 0.97 (0.94–0.99) for Alinity vs. Roche, 0.95 (0.92–0.99) for PathFast vs. Roche, and 0.94 (0.91–0.98) for PathFast vs. Alinity. For outpatients, the concordance was 0.92 (0.84–1.00) for Alinity vs. Roche, 0.90 (0.80–1.00) for PathFast vs. Roche, and 0.97 (0.92–1.00) for PathFast vs. Alinity.</div></div><div><h3>Conclusion</h3><div>Three NT-proBNP assay platforms demonstrated high correlation, minimal bias, and high clinical concordance. The results support the clinical interchangeability of these assays at commonly used and guideline-endorsed cutpoints.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"141 ","pages":"Article 111062"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145707534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorrectly prepared formula contributing to severe hypovolemic hypernatremia in enteral tube-fed children: A report of two cases","authors":"Lawrence de Koning ,&nbsp;Shelina M. Jamal ,&nbsp;Catherine Ross ,&nbsp;Michael J. Esser ,&nbsp;Erin Pols","doi":"10.1016/j.clinbiochem.2025.111040","DOIUrl":"10.1016/j.clinbiochem.2025.111040","url":null,"abstract":"<div><h3>Case Presentations</h3><div>We describe two cases of children who were significantly impacted by incorrectly prepared, hyperosmolar formula delivered via enteral tubes. The first case is of a 5-month-old post-pancreatectomy patient with complex needs who received hyperosmolar formula while in a pediatric intensive care unit (PICU), whereas the second is of a 1-year-old with spinal muscular atrophy who received hyperosmolar formula at home – resulting in a PICU admission.</div></div><div><h3>Discussion</h3><div>Hypovolemic hypernatremia is an important but poorly understood complication that can occur if overly concentrated formula is fed to children. Risk is almost certainly greater in enteral-tube fed patients because of their medical fragility but also because tube-feeding delivers formula directly and potentially very rapidly to the gastrointestinal system.</div></div><div><h3>Conclusions</h3><div>Formula for medically fragile, enteral tube-fed children should be prepared with utmost care – particularly when family caregivers are responsible for home feeding. In these cases, additional education and feeding support should be considered – including provision of pre-mixed formula. The clinical biochemistry laboratory can verify formula osmolality for these high-risk patients.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"141 ","pages":"Article 111040"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145512135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of mean platelet component and mean platelet mass in immune thrombocytopenia versus hypoproductive thrombocytopenias","authors":"Hrvoje Melinscak ,&nbsp;Tahir Mirzoyev ,&nbsp;Yu-Tong Hong ,&nbsp;Mala Varma","doi":"10.1016/j.clinbiochem.2025.111070","DOIUrl":"10.1016/j.clinbiochem.2025.111070","url":null,"abstract":"<div><h3>Introduction</h3><div>The Siemens (Bayer) ADVIA 120 has the capacity to calculate the mean platelet component (MPC), a measure of platelet density, and the mean platelet mass (MPM). In September 2013, we initiated a prospective study to determine if the MPC and MPM are significantly higher in immune thrombocytopenia than in hypoproductive thrombocytopenias.</div></div><div><h3>Methods</h3><div>Lavender tri-potassium EDTA tubes were filled and analyzed on the Siemens (Bayer) ADVIA 120 within a period not exceeding two hours from their collection. The student’s <em>t</em>-test was used to compare these parameters in the patient groups. Coefficients of variation were used to study intra-individual variability and inter-individual variability.</div></div><div><h3>Results</h3><div>Twenty patients with immune thrombocytopenia and 20 patients with hypoproductive thrombocytopenias were enrolled. The MPC and MPM were significantly higher in immune thrombocytopenia than in hypoproductive thrombocytopenias. A unique patient was studied when he had immune thrombocytopenia and then when he had chemotherapy-induced thrombocytopenia during treatment of lymphoma. His MPC and MPM were significantly higher during immune thrombocytopenia than during chemotherapy-induced thrombocytopenia. Intra-individual variability and inter-individual variability were low in this study.</div></div><div><h3>Conclusion</h3><div>A strength of our study is that the MPC and MPM assays were measured within 2 h of collection, since the MPC decreases over time. Our results attest to the clinical utility of MPC and MPM toward distinguishing immune thrombocytopenia from hypoproductive thrombocytopenias.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"141 ","pages":"Article 111070"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145827171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SeraSeq reference materials support non-invasive cfDNA screening methods","authors":"Sylvie Giroux ,&nbsp;Seyedeh Saideh Daryabari ,&nbsp;André Caron ,&nbsp;François Rousseau","doi":"10.1016/j.clinbiochem.2025.111046","DOIUrl":"10.1016/j.clinbiochem.2025.111046","url":null,"abstract":"<div><h3>Background</h3><div>Reference materials are essential for the validation, verification, and implementation of clinical assays. Seracare has developed the Seraseq line—commercial reference standards for non-invasive prenatal testing (NIPT)—which are designed to simulate maternal plasma containing cell-free DNA (cfDNA).</div></div><div><h3>Objective</h3><div>This study aims to evaluate the performance of Seraseq reference materials compared to natural maternal plasma, particularly assessing their quality and reliability as reference standards in NIPT workflows, both with and without size selection to enrich fetal fraction.</div></div><div><h3>Methods</h3><div>We analyzed six replicates from eight different Seraseq genotypes. cfDNA was extracted, prepared into sequencing libraries, and sequenced following the same protocols used for natural plasma samples. Size-selection was also applied to enrich shorter cfDNA fragments. The key performance metrics included cfDNA yield and integrity, library preparation efficiency, sequencing quality, fetal fraction estimation, and detection of aneuploidies and sex chromosome abnormalities.</div></div><div><h3>Results</h3><div>cfDNA quantity and quality from Seraseq materials were comparable to natural plasma. The materials yielded consistent library concentrations. Sequencing showed reliable detection of all targeted aneuploidies except the microdeletion del22q11, even with elevated fetal fraction. Size-selection increased fetal fraction and improved Z-scores for aneuploidy detection across all genotypes. Fragment size profiles exhibited slight but consistent deviations from natural plasma, notably after size selection.</div></div><div><h3>Conclusions</h3><div>Seraseq reference materials closely mimic the cfDNA characteristics of maternal plasma and perform reliably across multiple testing dimensions. While they may present slight differences in fragment size periodicity, these did not affect analytical performance. These materials are therefore suitable for validating and monitoring NIPT workflows.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"141 ","pages":"Article 111046"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145512062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical Imprecision and Therapeutic Intervals for Lithium – Are There Implications for Old Age Patients with Bipolar Disorder?","authors":"Janet Zhou ,&nbsp;Demitra Tsoukalas ,&nbsp;Nethra Chittiprol ,&nbsp;Peter Kavsak ,&nbsp;Felix Leung ,&nbsp;Jennifer Taher ,&nbsp;Zahraa Ali-Mohammed ,&nbsp;Saranya Arnoldo ,&nbsp;Heather Tysick ,&nbsp;Lei Fu ,&nbsp;Sara A. Love ,&nbsp;Rajeevan Selvaratnam","doi":"10.1016/j.clinbiochem.2025.111057","DOIUrl":"10.1016/j.clinbiochem.2025.111057","url":null,"abstract":"<div><h3>Background</h3><div>Monitoring blood levels of lithium is important for maintaining therapeutic efficacy. Age-dependent therapeutic intervals have been recommended by the International Society for Bipolar Disorder (ISBD) task force, 0.4–0.8 mmol/L and 0.4–0.7 mmol/L for adults aged 60–79 and ≥80 years old, respectively. However, the suitability of common colorimetric methods for lithium measurements in the suggested therapeutic intervals has not been characterized, nor has the proposed therapeutic intervals been confirmed with real-world patient data.</div></div><div><h3>Methods</h3><div>Serum samples spiked with various concentrations of lithium ranging from 0 to 3.5 mmol/L were analyzed on six different methods for imprecision and relative comparability of lithium measurements. An indirect reference interval approach using refineR was employed to derive therapeutic limits using patient results spanning 5 years and compared with the therapeutic intervals recommended by the ISBD task force.</div></div><div><h3>Results</h3><div>Routinely employed colorimetric methods for lithium measurements have sufficient precision within the recommended therapeutic intervals, although select colorimetric methods are notably more imprecise at 0.4 mmol/L. The derived therapeutic intervals using patient data align with those proposed by the ISBD task force for older adults.</div></div><div><h3>Conclusion</h3><div>Routine colorimetric methods used for lithium measurements have adequate precision and detection capabilities in the therapeutic windows recommended by the ISBD task force. In addition, the proposed therapeutic intervals are verifiable by real-world patient data.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"141 ","pages":"Article 111057"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145548427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel fluorescent-labeled serum protein electrophoresis method for detection of monoclonal free light chains in serum","authors":"Gaoyang Pang ,&nbsp;Xiaojun Kang ,&nbsp;Yan Li ,&nbsp;Xiaojuan Zhang ,&nbsp;Huijun Ren","doi":"10.1016/j.clinbiochem.2025.111067","DOIUrl":"10.1016/j.clinbiochem.2025.111067","url":null,"abstract":"<div><h3>Background</h3><div>Current guidelines recommend a combination of serum protein electrophoresis (SPE), immunofixation electrophoresis (IFE), and serum free light chain (FLC) assays for monoclonal immunoglobulin (M−protein) screening. However, the high cost of this comprehensive panel limits its widespread use. In routine clinical practice in China, SPE alone is commonly employed for general population screening, but it suffers from low sensitivity for detecting monoclonal free light chains (LC-M−proteins), leading to a high rate of missed diagnoses. There is a clear clinical need for a novel method that enhances LC-M−protein detection while preserving the practicality of SPE.</div></div><div><h3>Methods</h3><div>We established a fluorescent-labeled serum protein electrophoresis (FSPE) technique. The method utilizes 2-(diphenylphosphino)ethylamine (DPEA) to selectively reduce the C-terminal thiols of LC-M−proteins (4 °C, 60 min, dark), followed by specific fluorescent labeling with sulfo-Cyanine5 maleimide (4 °C, 30 min, dark). After agarose gel electrophoresis separation, synchronous identification and quantification of LC-M−proteins and intact M−proteins are achieved via dual-channel (fluorescence and protein staining) imaging.</div></div><div><h3>Results</h3><div>Method validation demonstrated that DPEA enables efficient and selective reduction. The detection limit of FSPE for LC-M−proteins reached 400 mg/L. Quantitative analysis showed excellent performance (linearity: R2 = 0.9887; total precision: relative standard deviation, RSD = 6.61%). FSPE results were highly correlated with the reference Freelite™ FLC assay (Spearman’s r_s = 0.895, p &lt; 0.01), although Bland-Altman analysis revealed quantitative differences between individual samples.</div></div><div><h3>Conclusion</h3><div>FSPE represents a significant upgrade to conventional SPE, effectively addressing its critical flaw of missing LC-M−proteins while achieving sensitivity comparable to IFE. It retains the key advantages of SPE, namely operational simplicity and low cost. Therefore, FSPE emerges as a potential standalone method for efficient M−protein screening.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"141 ","pages":"Article 111067"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparability of venous and capillary blood measurements for a host-protein test differentiating bacterial from viral infections","authors":"Mary Hainrichson ,&nbsp;Nitzan Shamir ,&nbsp;Naftalie Senderovich ,&nbsp;May Levin ,&nbsp;Husein Darawsha ,&nbsp;Ayelet Raz ,&nbsp;Salim Halabi ,&nbsp;Oded Shaham ,&nbsp;Roy Navon ,&nbsp;Tanya M. Gottlieb ,&nbsp;Barak Hershkovitz","doi":"10.1016/j.clinbiochem.2025.111065","DOIUrl":"10.1016/j.clinbiochem.2025.111065","url":null,"abstract":"<div><h3>Background</h3><div>MeMed BV is a host-protein test for discriminating bacterial and viral infections. It was analytically and clinically validated in serum and venous whole blood samples. Here we investigated the comparability of venous versus capillary blood measurements of the MeMed BV score and of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), interferon gamma-induced protein-10 (IP-10), and C-reactive protein (CRP) biomarkers.</div></div><div><h3>Methods</h3><div>Adult patients with suspected acute infection were enrolled prospectively at three medical centers. Eligibility criteria were based on MeMed BV instructions for use. Paired venous and capillary samples (50 µL) were collected and measured.</div><div>Three predetermined acceptance criteria for comparability for MeMed BV score were: (1) Slope 0.9–1.1 and intercept −5 to 5 in Passing-Bablok regression; (2) 95 % confidence intervals (CI) for bias at score bin cutoffs fall within −12.5 to 12.5; and (3) &lt;5 % of paired scores fall in non-adjacent clinical interpretation bins.</div></div><div><h3>Results</h3><div>Among 58 patients (median age 58.5 years), TRAIL, IP-10, and CRP measurements in venous and capillary blood were highly correlated (r = 0.98–0.99). For the MeMed BV score, slope was 1.00 (95 % CI: 0.99, 1.01), intercept 0.00 (−1.04, 0.08), 95 % CI for bias at each cutoff was within limits and no paired scores fell in non-adjacent bins. All three criteria were met and findings were consistent in a single replicates analysis.</div></div><div><h3>Conclusion</h3><div>Venous and capillary blood yield comparable MeMed BV scores and biomarker values. The findings can serve as the basis for expanding MeMed BV’s use to include capillary blood specimens, potentially widening its clinical utility.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"141 ","pages":"Article 111065"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145707483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}