Physiological Plant Pathology最新文献

Evidence that a putative necrotoxin of Erwinia amylovora is an artifact caused by the activity of inorganic salts 有证据表明，一种假定的淀粉状欧文氏菌的坏死性毒素是由无机盐的活性引起的

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90042-6

D.W. Bauer, S.V. Beer

{"title":"Evidence that a putative necrotoxin of Erwinia amylovora is an artifact caused by the activity of inorganic salts","authors":"D.W. Bauer, S.V. Beer","doi":"10.1016/0048-4059(85)90042-6","DOIUrl":"10.1016/0048-4059(85)90042-6","url":null,"abstract":"<div>Pear cell-suspension cultures (PCSC) inoculated with pathogenic strains of Erwinia amylovora developed necrosis in 4–10 days. Necrotoxin preparations, produced by fractionation of necrotic PCSC, induced necrosis of pear seedling cuttings and inhibited the growth of PCSC. Fractionation of PCSC medium yielded a fraction that induced similar responses. Solutions of the inorganic salts used in the medium, similar in electrical conductivity to the necrotoxin preparations, affected pear seedling cuttings and PCSC in a manner that was indistinguishable from that of necrotoxin preparations. Toxic activity was retained following destruction of the organic compounds of a necrotoxin preparation by ashing. Upon fractionation of necrotoxin preparations by gel-permeation chromatography, all toxic activity migrated with the low molecular weight fractions that contained inorganic salts. Therefore, the toxic activity of the necrotoxin preparation was due to the inorganic salts from the tissue culture medium, concentrated to toxic levels by the purification procedures. No phytotoxin of bacterial origin was found in our preparations.</div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"27 3","pages":"Pages 289-298"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90042-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72653701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effect of induced resistance on the efficiency of powdery mildew haustoria in wheat and barley 诱导抗性对小麦和大麦白粉病吸器效率的影响

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90048-7

K. Stenzel, Ulrke Steiner, F. Schönbeck

{"title":"Effect of induced resistance on the efficiency of powdery mildew haustoria in wheat and barley","authors":"K. Stenzel, Ulrke Steiner, F. Schönbeck","doi":"10.1016/0048-4059(85)90048-7","DOIUrl":"10.1016/0048-4059(85)90048-7","url":null,"abstract":"<div>Resistance against Erysiphe graminis was induced in wheat and barley by treating the plants with metabolites from a selected isolate of Bacillus subtilis. The induced resistance did not affect conidial germination or the formation of appressoria, but it did reduce the formation of primary haustoria and the number of colonies which subsequently developed. The colonies formed were smaller with less mycelium per unit area of leaf than the colonies on untreated plants. Fewer haustoria and conidiophores were produced per unit area of colony, and numbers of conidia per conidiophore and per haustorium were also reduced. In terms of the quantity of fungal structures formed per haustorium, the efficiency of the haustoria was reduced by 46% by the induced resistance. A higher percentage of haustoria in the induced resistant plants showed an enlarged extrahaustorial matrix, although the structure of the haustoria themselves was not altered. The results suggest that the resistance induced by treatment with the microbial metabolites impairs the development of E. graminis in much the same way as genetically determined partial resistance.</div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"27 3","pages":"Pages 357-367"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90048-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75690939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Differential sensitivity of Phytophthora megasperma f.sp. glycinea isolates to glyceollin isomers 大芽疫霉的差异敏感性。甘氨酸分离物为甘油异构体

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90043-8

M.K. Bhattacharyya, E.W.B. Ward

{"title":"Differential sensitivity of Phytophthora megasperma f.sp. glycinea isolates to glyceollin isomers","authors":"M.K. Bhattacharyya, E.W.B. Ward","doi":"10.1016/0048-4059(85)90043-8","DOIUrl":"10.1016/0048-4059(85)90043-8","url":null,"abstract":"<div>Glyceollin isomers I, II and III were extracted from soybean cotyledons (cv. Harosoy 63) inoculated with Phytophthora megasperma f.sp. glycinea race 1, and separated and purified by column, high performance and thin layer chromatography and quantitated by spectrophotometry. The extinction coefficient of glyceollin I was 10 800, that of glyceollin II and III conformed to published values. Glyceollin I (ED50 approx. 33 μg ml−1) was almost twice as inhibitory as glyceollin II and III to growth of P. megasperma f.sp. glycinea race 1 on V8 juice agar. All three isomers were less active in soybean hypocotyl extract agar. Glyceollin II (ED50 7 μg ml−1) was the most active against zoospore germination, and caused zoospores to burst; ED50 values for glyceollin I and III were 12·2 and 13·9 μg ml−1 respectively. Three isolates (1·1, 1·2, 1·3) were obtained from sectors in colonies on agar medium amended with glyceollin I, II or III respectively. Isolates 1·1 and 1·3 grew more rapidly on medium amended with glyceollin I, III or a natural glyceollin mixture than did wild type P. megasperma fsp. glycinea race I. All isolates and wild type had similar growth rates on medium amended with glyceollin II. The isolates did not lose their tolerance when grown on control medium and are presumably genetically controlled variants. Tolerance of glyceollin did not increase the aggressiveness of the isolates on soybeans.</div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"27 3","pages":"Pages 299-310"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90043-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74933520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Phenylalanine ammonia-lyase and susceptibility of the maize mesocotyl to Helminthosporium maydis 苯丙氨酸解氨酶及玉米中胚轴对麦氏线虫的敏感性

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90047-5

S.F. Pascholati, D. Heim, R.L. Nicholson

引用次数: 8

NADPH-dependent O2− generation in membrane fractions isolated from wounded potato tubers inoculated with Phytophthora infestans 接种疫霉后马铃薯损伤块茎膜组分中nadph依赖的O2 -生成

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90044-X

N. Doke

{"title":"NADPH-dependent O2− generation in membrane fractions isolated from wounded potato tubers inoculated with Phytophthora infestans","authors":"N. Doke","doi":"10.1016/0048-4059(85)90044-X","DOIUrl":"10.1016/0048-4059(85)90044-X","url":null,"abstract":"<div>A membrane-rich fraction from wounded potato tubers showed increasing activity of NADPH-dependent cytochrome c, but not of acetylated cytochrome c reducing activity, during ageing after slicing (wound-induced activity, WIA). In the fraction from aged tissues inoculated with an incompatible, but not a compatible, race of Phyiophthora infestans, as increase in native and acetylated cytochrome c reducing activities was closely associated with the occurrence of hypersensitive cell death and phytoalexin production (infection-induced activity, IIA). Treatment of aged tissues with hyphal wall components (HWC), a hypersensitivity-eliciting factor of the fungus, also activated both native and acetylated cytochrome c reducing activities similarly to infection. IIA and WIA were inhibited by NADP+, but only the former was appreciably inhibited by superoxide dismutase (SOD).Water-soluble glucans (WSG), a hypersensitivity-inhibiting factor from compatible, but not incompatible, races of P. infestans, significantly inhibited the IIA but not the WIA in vitro.These results suggest that a novel O2− generating NADPH oxidase system in the membrane of potato tissues may be activated following an incompatible cell reaction, which results in hypersensitive cell death and phytoalexin production. The lack of IIA activation in the compatible interaction may result from an inhibition of the reaction system by the water-soluble glucans from the fungus, thus resulting in the establishment of a compatible interaction.</div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"27 3","pages":"Pages 311-322"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90044-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77069761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 217

Activation of superoxide generation and enhancement of resistance against compatible races of Phytophthora infestans in potato plants treated with digitonin 洋地黄苷对马铃薯超氧化物生成的激活及对马铃薯疫霉亲和小种的抗性增强

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90045-1

N. Doke, H.B. Chai

{"title":"Activation of superoxide generation and enhancement of resistance against compatible races of Phytophthora infestans in potato plants treated with digitonin","authors":"N. Doke, H.B. Chai","doi":"10.1016/0048-4059(85)90045-1","DOIUrl":"10.1016/0048-4059(85)90045-1","url":null,"abstract":"<div>Application of digitonin to the leaf surface of potato plants was demonstrated to activate O2− generation of the leaf tissues as determined by extracellular cytochrome c reduction which was inhibited by superoxide dismutase (SOD). Protoplasts prepared from stem shoots of potato enhanced NADPH-dependent reduction of extracellular cytochrome c immediately after being treated with digitonin. The reduction was also inhibited by SOD. The similar activation of SOD-sensitive cytochrome c reducing activity was observed in leaves or protoplasts of some other plants treated with digitonin.Potato leaf tissues pre-treated with digitonin by application to the upper or lower surfaces, or through the petiole, were protected from disease caused by infection with compatible races of Phytophthora infestans. Treatment of the wound surface ofpotato tuber tissue with digitonin induced the generation of the superoxide anion and, at the same time, sporangial germination, appressorial formation and invasion by P. infestans was greatly reduced. This effect was partially negated by the presence of SOD in the inoculum and seemed not to depend on antifungal compounds.These results suggest that plant tissues possess an O2− generating NADPH oxidase which is activated by digitonin and that its activation in potato may contribute to an enhanced resistance against attack by compatible races of P. infestans at pre- or post-infection stages.</div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"27 3","pages":"Pages 323-334"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90045-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73541179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 63

Elicitation of cotton isoperoxidases by Aspergillus flavus and other fungi pathogenic to cotton 黄曲霉及其他棉花病原真菌诱导棉花异过氧化物酶的研究

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90041-4

Jay E. Mellon, Louise S. Lee

{"title":"Elicitation of cotton isoperoxidases by Aspergillus flavus and other fungi pathogenic to cotton","authors":"Jay E. Mellon, Louise S. Lee","doi":"10.1016/0048-4059(85)90041-4","DOIUrl":"10.1016/0048-4059(85)90041-4","url":null,"abstract":"<div>The distribution of peroxidase(s) in non-inoculated cotton bolls and flowers was qualitatively determined. The soluble and bound forms of cotton peroxidase(s) were investigated for changes elicited by fungal infection. Inoculation of cotton bolls, 30 days post anthesis, with Aspergillus flavus, Fusarium equiseti, Fusarium moniliforme, Fusarium semitectum or Rhizoctonia solani followed by a 6-day incubation period stimulated soluble peroxidase activity by 2- to 6-fold. In addition, fungal stress elicited two to five distinct soluble peroxidase isozymes not observed or at very low levels in non-inoculated controls. The pl values by isoelectric focusing for these isoperoxidases were 4·25, 4·35, 4·50, 4·65, 4·75 and 4·83. The pI 4·50 form was produced in controls and was also the major isozyme in the stress profiles. Each cotton-pathogen combination produced a unique peroxidase profile. No differences between control and fungal-stressed bolls were observed with either the specific activity or the peroxidase profile in the case of ionically-bound or covalently-bound classes of the enzyme.</div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"27 3","pages":"Pages 281-288"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90041-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83711612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Temperature-sensitivity of the expression of resistance to Puccinia graminis conferred by the Sr15, Sr9b and Sr14 genes in wheat Sr15、Sr9b和Sr14基因对小麦小麦锈病抗性表达的温度敏感性

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90046-3

H.D.M. Gousseau , B.J. Deverall, R.A. McIntosh

引用次数: 32

Cytological studies of the interactions between the cowpea rust fungus and silicon-depleted French bean plants 豇豆锈菌与缺硅法国豆植物相互作用的细胞学研究

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90049-9

Mary Ann Stumpf, Michèle C. Heath

{"title":"Cytological studies of the interactions between the cowpea rust fungus and silicon-depleted French bean plants","authors":"Mary Ann Stumpf, Michèle C. Heath","doi":"10.1016/0048-4059(85)90049-9","DOIUrl":"10.1016/0048-4059(85)90049-9","url":null,"abstract":"<div>To investigate the role of silica-rich wall deposits in resistance to the cowpea rust fungus, French bean plants were grown hydroponically in nutrient solutions supplemented with or depleted in silicon. Primary leaves supplied with adequate silicon responded to fungal infection by the autofluorescence of guard cell walls, the limited autofluorescence of mesophyll cell walls (both visualized in cleared tissue), and the deposition in and on the latter of silica. If infected, silicon-depleted plants, light microscopy, electron microscopy, and energy dispersive X-ray analysis indicated that silica deposits were absent. The incidence of autofluorescence of guard cells was comparable to that in the silicon-supplemented plants, but the incidence and extent of mesophyll wall autofluorescence was greatly enhanced. The autofluorescence of mesophyll cells, but not guard cells, corresponded to areas of the wall that gave a colour reaction with toluidine blue indicative of phenolic compounds. Callose (aniline blue positive material), in the form of papillae, was also extremely common at infection sites in silicon-depleted leaves. Infection hyphae rarely formed haustoria in either silicon-depleted plants or those given adequate silicon, although these hyphae grew as well, and appeared equally healthy, in both types of plants. In silicon-depleted plants, pre-inoculation heat treatments or injection of intercellular fluids from bean rust-infected bean leaves, increased the incidence of haustorium formation and decreased the incidence of all observed plant responses. The results suggest that either silica deposition is not the primary barrier to haustorium formation in normal plants, or that a second barrier, such as the impregnation of the plant wall with phenolic materials, comes into play if silica deposition is prevented.</div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"27 3","pages":"Pages 369-385"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90049-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82600253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Photosynthesis and chloroplast functioning within individual pustules of Uromyces muscari on bluebell leaves 风信子叶片上蕈蕈单个脓疱内的光合作用和叶绿体功能

Physiological Plant Pathology Pub Date : 1985-11-01 Epub Date: 2008-07-21 DOI: 10.1016/0048-4059(85)90050-5

J.D. Scholes, J.F. Farrar

{"title":"Photosynthesis and chloroplast functioning within individual pustules of Uromyces muscari on bluebell leaves","authors":"J.D. Scholes, J.F. Farrar","doi":"10.1016/0048-4059(85)90050-5","DOIUrl":"10.1016/0048-4059(85)90050-5","url":null,"abstract":"<div>Photosynthesis and chloroplast functioning is examined within pustules of Uromyces muscari on bluebell leaves at different stages of development from presporulation to green island formation. Green island tissue is shown to be photosynthetically active by 14CO2 fixation, OZ evolution and in vivo chlorophyll fluorescence kinetics. Chloroplast measurements indicate that, whilst there is little reduction in chloroplast number per unit area in pustules of U. muscari, the chloroplast volume, the chlorophyll concentration and the ratio of chlorophyll a : b decline, suggesting that chlorophyll is lost from individual chloroplasts. Oxygen evolution, both per unit area and per unit chlorophyll, is reduced in pustules in comparison to green areas of an infected leaf and to uninfected leaves. Two major parameters of in vivo chlorophyll fluorescence kinetics (Fvar and F.) are progressively reduced in pustules. This suggests that non-cyclic electron transport, and general chloroplast integrity, are impaired as the disease develops. These results are discussed in the context of current theories concerning the mechanisms by which parasites induce a block in the electron transport chain, and a new model is proposed, based on a change in the chlorophyll content of the photosystem reaction centres.</div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"27 3","pages":"Pages 387-400"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90050-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86202613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39