Mutation Research/DNAging最新文献_第3页

Mutation frequency in human blood cells increases with age 人类血细胞的突变频率随着年龄的增长而增加

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00019-3

Mitoshi Akiyama , Seishi Kyoizumi , Yuko Hirai , Yoichiro Kusunoki , Keisuke S. Iwamoto , Nori Nakamura

引用次数: 92

Use of transgenic mouse models for studying somatic mutations in aging 利用转基因小鼠模型研究衰老过程中的体细胞突变

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00025-2

Hans-Jörg Martus , Martijn E.T. Dolle , Jan A. Gossen , Michaël E.T.I. Boerrigter , Jan Vijg

{"title":"Use of transgenic mouse models for studying somatic mutations in aging","authors":"Hans-Jörg Martus , Martijn E.T. Dolle , Jan A. Gossen , Michaël E.T.I. Boerrigter , Jan Vijg","doi":"10.1016/0921-8734(95)00025-2","DOIUrl":"10.1016/0921-8734(95)00025-2","url":null,"abstract":"<div><p>Theories on the causes of aging, based on the accumulation of somatic mutations in tissues of an organism, were formulated decades ago, but remain insufficiently tested. Transgenic animals, equipped with integrated bacterial reporter genes that can be efficiently rescued from total genomic DNA of all tissues and organs, represent ideal tools for investigating the types and frequencies of spontaneous mutants accumulating during aging. The first of such systems, based on the transgenic integration of bacteriophage lambda shuttle vectors that contain the bacterial lacZ gene as mutational target, was constructed in our laboratory and is now routinely used. Results obtained with this and the related LacI system that are relevant for the somatic mutation theory of aging will be discussed. One conclusion is that, due to the nature of the transgene. lambda-based systems have the disadvantage that deletion type mutations are underrepresented in comparison to point mutations. To overcome those limitations, we constructed a new transgenic mouse model carrying a pUR288 plasmid shuttle vector with the lacZ reporter gene. Some preliminary data obtained with this model serve to illustrate its potential use to extensively test the somatic mutation theory of aging.</p></div>","PeriodicalId":100937,"journal":{"name":"Mutation Research/DNAging","volume":"338 1","pages":"Pages 203-213"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0921-8734(95)00025-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18572754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

DNA damage, mutation and fine structure DNA repair in aging 衰老过程中DNA损伤、突变及精细结构DNA修复

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00008-T

Vilhelm A. Bohr, R. Michael Anson

引用次数: 186

Human aging is associated with stochastic somatic mutations of mitochondrial DNA 人类衰老与线粒体DNA的随机体细胞突变有关

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00021-W

Bernhard Kadenbach , Christof Münscher , Viola Frank , Josef Müller-Höcker , Jörg Napiwotzki

引用次数: 125

Impact of age and environment on somatic mutation at the hprt gene of T lymphocytes in humans 年龄和环境对人T淋巴细胞hprt基因体细胞突变的影响

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00018-2

Irene M. Jones , Cynthia B. Thomas , Bethany Tucker , Claudia L. Thompson , Pavel Pleshanov , Irena Vorobtsova , Dan H. Moore II

{"title":"Impact of age and environment on somatic mutation at the hprt gene of T lymphocytes in humans","authors":"Irene M. Jones , Cynthia B. Thomas , Bethany Tucker , Claudia L. Thompson , Pavel Pleshanov , Irena Vorobtsova , Dan H. Moore II","doi":"10.1016/0921-8734(95)00018-2","DOIUrl":"10.1016/0921-8734(95)00018-2","url":null,"abstract":"<div><p>Analysis of two human populations for dependence of somatic mutation on age has revealed both similarities and differences. The studies performed employed peripheral blood lymphocytes and measured the efficiency with which these cells form clones in vitro (cloning efficiency, CE) and the frequency of cells with inactivating mutations of the hypoxanthine phosphoribosyltransferase gene (mutant frequency, MF). The people studied were between 19 and 64 years of age. In one population, composed of 78 never smokers and 140 current smokers from the United States (US), both CE and MF were dependent on age: CE declined with age (<em>p</em> = 0.005); MF increased 0.15 per 10<sup>6</sup> cells per year of age for nonsmokers (<em>p</em> < 0.001) and at 1.3 times that rate for smokers (<em>p</em> = 0.01). In the second population, 80 people of unknown smoking status from Russia, the increase in MF per year was even greater, 2.5 times that of the US nonsmokers (<em>p</em> = 0.001) but the dependence of CE on age was the same as for the US population (<em>p</em> = 0.043). Because the increase of MF of the Russians with age is 2-fold greater than that of the US smokers, the intensity of smoking and/or other environmental exposures, or the susceptibility to these exposures, must account for the difference in age dependent MF increase, not the proportion of Russians that are smokers. Differences in the lymphocyte subpopulations that survived the longer transit from Russia may have contributed to the observed differences in MF. However, overall, the mutant frequency results suggest that the Russians were chronically exposed to higher levels of agents that induce somatic mutation and that, on an age adjusted basis, the Russia population studied is at increased risk for health consequences from accumulated genetic damage.</p></div>","PeriodicalId":100937,"journal":{"name":"Mutation Research/DNAging","volume":"338 1","pages":"Pages 129-139"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0921-8734(95)00018-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18572075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 74

P DNA element movement in somatic cells reduces lifespan in Drosophila melanogaster: evidence in support of the somatic mutation theory of aging 体细胞中P DNA元素的运动减少了黑腹果蝇的寿命:支持衰老体细胞突变理论的证据

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00009-U

R.C. Woodruff, A.G. Nikitin

{"title":"P DNA element movement in somatic cells reduces lifespan in Drosophila melanogaster: evidence in support of the somatic mutation theory of aging","authors":"R.C. Woodruff, A.G. Nikitin","doi":"10.1016/0921-8734(95)00009-U","DOIUrl":"10.1016/0921-8734(95)00009-U","url":null,"abstract":"<div><p>Evidence is presented in support of the hypothesis that P DNA element movement in somatic cells of <em>Drosophila melanogaster</em> induces genetic damage that significantly reduces lifespan. The lifespan of <em>D. melanogaster</em> males was significantly reduced by the somatic movement of a single P element in the presence of P[ry<sup>+</sup> Δ2–3](99B) transposase. In addition, the P[ry<sup>+</sup> SalI](89D) repressor of P[ry<sup>+</sup> Δ2–3](99B) somatic transposasc was observed to reduce the effect of P element movement on lifespan. Finally, the frequency of somatic-cell chromosome breakage was significantly increased in neuroblasts of males with somatically active P elements.</p><p>These results show that lifespan in <em>D. melanogaster</em> is decreased with increased somatic genetic damage from DNA-element movement. Although this conclusion does not confirm that transposable element movement is a cause of natural senescence, this conclusion is clear evidence in support of a close relationship between somatic genetic damage and aging.</p></div>","PeriodicalId":100937,"journal":{"name":"Mutation Research/DNAging","volume":"338 1","pages":"Pages 35-42"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0921-8734(95)00009-U","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18571033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Effect of aging on spontaneous micronucleus frequencies in peripheral blood of nine mouse strains: the results of the 7th collaborative study organized by CSGMT/JEMS · MMS 衰老对9种小鼠外周血自发微核频率的影响:CSGMT/JEMS·MMS第七次合作研究结果

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00011-T

Sci-ichi Sato , Masako Taketomi , Madoka Nakajima , Michiyo Kitazawa , Hiroyasu Shimada , Satoru Itoh , Miyuki Igarashi , Naohiko Higashikuni , Shizuyo Sutou , Yu F. Sasaki , Makoto Hayashi , Toshio Sofuni , Takafumi Higashiguchi , Shinji Nito , Yasushi Kondo , Sachiko Honda , Mikiko Hayashi , Yasuhiro Shinagawa , Eiichi Nakajima , Yoshie Oka , Koichi Tamai

{"title":"Effect of aging on spontaneous micronucleus frequencies in peripheral blood of nine mouse strains: the results of the 7th collaborative study organized by CSGMT/JEMS · MMS","authors":"Sci-ichi Sato , Masako Taketomi , Madoka Nakajima , Michiyo Kitazawa , Hiroyasu Shimada , Satoru Itoh , Miyuki Igarashi , Naohiko Higashikuni , Shizuyo Sutou , Yu F. Sasaki , Makoto Hayashi , Toshio Sofuni , Takafumi Higashiguchi , Shinji Nito , Yasushi Kondo , Sachiko Honda , Mikiko Hayashi , Yasuhiro Shinagawa , Eiichi Nakajima , Yoshie Oka , Koichi Tamai","doi":"10.1016/0921-8734(95)00011-T","DOIUrl":"10.1016/0921-8734(95)00011-T","url":null,"abstract":"<div><p>The spontaneous frequencies of micronucleated reticulocytes (MNRETs) were examined monthly over the life spans of animals belonging to nine mouse strains for the 7th collaborative study organized by the CSGMT/JEMS MMS. Both sexes of the BDF1 strain and females of the A/J strain showed a statistically significant increase in mean spontaneous MNRET frequency in their last month of life, suggesting the possibility of strain-specific, age-dependent chromosomal instability. SAMP6/Tan, an accelerated senescence-prone strain, showed the same tendency, although it was not statistically significant. The other strains studied, ddY, CD-1, B6C3F1, SAMR1, and MS/Ac, did not show significant age-related differences in mean of MNRET frequencies. More extensive statistical analyses are underway, and the outcomes will be reported separately.</p></div>","PeriodicalId":100937,"journal":{"name":"Mutation Research/DNAging","volume":"338 1","pages":"Pages 51-57"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0921-8734(95)00011-T","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18571035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Spontaneous mutant frequency of lacZ gene in spleen of transgenic mouse increases with age 转基因小鼠脾脏lacZ基因自发突变频率随年龄增加而增加

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00023-Y

T. Ono , Y. Miyamura , H. Ikehata , H. Yamanaka , A. Kurishita , K. Yamamoto , T. Suzuki , T. Nohmi , M. Hayashi , T. Sofuni

引用次数: 45

Factors affecting somatic mutation frequencies in vivo 体内影响体细胞突变频率的因素

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00024-Z

X.B. Zhang, C. Urlando, K.S. Tao , J.A. Heddle

{"title":"Factors affecting somatic mutation frequencies in vivo","authors":"X.B. Zhang, C. Urlando, K.S. Tao , J.A. Heddle","doi":"10.1016/0921-8734(95)00024-Z","DOIUrl":"10.1016/0921-8734(95)00024-Z","url":null,"abstract":"<div><p>The factors that influence the spontaneous mutant frequencies in mammalian tissues have been ranked on the basis of data from our laboratory together with published data. Some of the data come from the endogenous <em>hprt</em> and <em>Dlb-1</em> loci, but most come from transgenic mice carrying the bacterial <em>lad</em> and <em>lacI</em> genes in recoverable lambda phage vectors. Since there is evidence that these bacterial loci are selectively neutral, the mutant frequency observed is the integral of the mutation rates from the formation of the zygote. The factors that affect the inferred mutation rate, in decreasing order of importance are: site of integration of the transgene, age, tissue, and strain. Insufficient data exist to determine the influence of gender (probably small) and inter-laboratory variables (probably at least as important as age). The two most surprising results arc (1) that about half of all mutations arise during development (and half of these in utero) and (2) that most somatic tissues, whether queiscent or actively proliferating, have similar mutant frequencies and similar increases during adult life.</p></div>","PeriodicalId":100937,"journal":{"name":"Mutation Research/DNAging","volume":"338 1","pages":"Pages 189-201"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0921-8734(95)00024-Z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18572752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 50

Nucleic acid fingerprinting by PCR-based methods: applications to problems in aging and mutagenesis 基于pcr的核酸指纹图谱方法:在衰老和突变问题中的应用

Mutation Research/DNAging Pub Date : 1995-10-01 Epub Date: 2003-03-31 DOI: 10.1016/0921-8734(95)00026-3

John Welsh, Nick Rampino, Michael McClelland, Manuel Perucho

{"title":"Nucleic acid fingerprinting by PCR-based methods: applications to problems in aging and mutagenesis","authors":"John Welsh, Nick Rampino, Michael McClelland, Manuel Perucho","doi":"10.1016/0921-8734(95)00026-3","DOIUrl":"10.1016/0921-8734(95)00026-3","url":null,"abstract":"<div><p>There are many methods of inference in common use in biology that are based on population sampling, including such diverse areas as sampling organisms to determine the population structure of an ecosystem, sampling a set of DNA sequences to infer evolutionary history, sampling genetic loci to build a genetic map, sampling differentially expressed genes to find phenotypic markers, and many others. Recently developed PCR-based methods for nucleic acid fingerprinting can be used as sampling tools with general applicability in molecular biology, evolution and genetics. These methods include arbitrarily primed PCR (AP-PCR; <span>Welsh and McClelland, 1990</span>) and random amplified polymorphic DNA (RAPD; <span>Williams et al., 1990</span>) for the fingerprinting of DNA, and RNA arbitrarily primed PCR (RAP-PCR; <span>Welsh et al., 1992a</span>) and differential display (DD; <span>Liang and Pardee, 1992</span>) for the fingerprinting of RNA. Novel ways of looking at genetic control are facilitated by the high data-acquisition capabilities of the fingerprinting methods. In this article, we review some of the applications of DNA fingerprinting to the study of mutagenesis, and of RNA fingerprinting to the study of normal and abnormal signal transduction. We propose that these fingerprinting approaches may also have applications in the study of senescence and aging.</p></div>","PeriodicalId":100937,"journal":{"name":"Mutation Research/DNAging","volume":"338 1","pages":"Pages 215-229"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0921-8734(95)00026-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18572755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34