Mutation Research/DNA Repair Reports最新文献_第7页

Reparative strand incision in saponin-permeabilized human fibroblasts 皂苷渗透人成纤维细胞的修复性链切口

Mutation Research/DNA Repair Reports Pub Date : 1987-11-01 DOI: 10.1016/0167-8817(87)90022-8

William K. Kaufmann, Linda P. Briley

{"title":"Reparative strand incision in saponin-permeabilized human fibroblasts","authors":"William K. Kaufmann, Linda P. Briley","doi":"10.1016/0167-8817(87)90022-8","DOIUrl":"10.1016/0167-8817(87)90022-8","url":null,"abstract":"<div>The damage-directed strand incision step in the nucleotidyl DNA excision-repair pathway (NDERP) was characterized in quiescent monolayer cultures of human fibroblasts in which the plasma membrane was selectively permeabilized with saponin. When permeable normal human fibroblasts (NHF) were incubated in a DNA-repair assay mixture lacking the deoxyribonucleoside triphosphate precursors, the numbers of UV-dependent DNA-strand breaks were increased by about 9-fold consistent with the uncoupling of incision from gap-filling DNA synthesis and ligation. In uncoupled NHF omission of ATP reduced the numbers of UV-dependent strand breaks by 84% confirming the requirement for ATP for reparative strand incision. Time-course experiments indicated that the maximum rate of strand incision occurred in the first 10 min of incubation of permeable cells and diminished to 16–28% of this rate between 30 and 60 min of incubation. The initial rate of incision in permeable NHF was estimated to be 20% of that seen in intact fibroblasts. Dose-response studies indicated an initial saturation of strand incision activity at fluences between 10 and 25 J/m2. In permeable group A xeroderma pigmentosum fibroblasts (XPA) few UV-dependent incisions were produced after 10–25 J/m2. In the xeroderma pigmentosum variant (XPV) strain that we studied, strand incisions saturated at a plateau level that was about twice that seen in the NHF strain suggesting the preservation of a higher level of incision activity after permeabilization. After fluences above 50 J/m2 additional strand incision was observed in all cell strains reflecting the activity of a damage-dependent endodeoxyribonuclease that is independent of the NDERP. Saponin-treated fibroblasts were also permeable to pancreatic deoxyribonuclease I and the UV-DNA endonuclease from M. luteus indicating that these preparations may be used for in vitro complementation.</div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(87)90022-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14790608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Enzymatic studies of DNA repair in Drosophila melanogaster 果蝇DNA修复的酶学研究

Mutation Research/DNA Repair Reports Pub Date : 1987-11-01 DOI: 10.1016/0167-8817(87)90018-6

Walter A. Deutsch

引用次数: 7

Effects of the Escherichia coli recF suppressor mutation, recA801, on recF-dependent DNA-repair associated phenomena 大肠杆菌recF抑制基因突变recA801对recF依赖性dna修复相关现象的影响

Mutation Research/DNA Repair Reports Pub Date : 1987-11-01 DOI: 10.1016/0167-8817(87)90015-0

Michael R. Volkert , Margaret A. Hartke

{"title":"Effects of the Escherichia coli recF suppressor mutation, recA801, on recF-dependent DNA-repair associated phenomena","authors":"Michael R. Volkert , Margaret A. Hartke","doi":"10.1016/0167-8817(87)90015-0","DOIUrl":"10.1016/0167-8817(87)90015-0","url":null,"abstract":"<div>In recB recC sbcB mutants genetic recombination is dependent upon the recF gene. recA801, recA802 and recA803 (formerly called srfA mutations) were originally isolated as mutations that suppress recombination deficiency caused by a recF mutation in a recB recC sbcB genetic background. Since the recA801 mutation also suppressed some of the UV sensitivity due to recF143, we sought to determine what DNA-repair pathways were actually being restored by the recA801 mutation in this genetic background. In this paper we show that the suppression of recF143 by recA801 does not extend to the recF143-mediated defects in induced repair of UV-damaged phages. In addition, we show that recA801 suppresses only slightly the recF143-associated defect in induced expression of the SOS-regulated muc genes of pKM101. These results suggest that recA801 suppresses primarily the RecF pathway of recombinational repair.</div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(87)90015-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13961381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Unique cross-link and monoadduct repair characteristics of a xeroderma pigmentosum revertant cell line 独特的交联和单加合修复特性着色性干皮病逆转细胞系

Mutation Research/DNA Repair Reports Pub Date : 1987-11-01 DOI: 10.1016/0167-8817(87)90024-1

Ljiljana Vuksanovic, James E. Cleaver

引用次数: 29

Defective DNA excision repair in cells of patients with homocystinuria 同型半胱氨酸尿患者细胞DNA切除修复缺陷

Mutation Research/DNA Repair Reports Pub Date : 1987-11-01 DOI: 10.1016/0167-8817(87)90025-3

T.A. Sinelshchikova, G.N. Lvova, N.N. Shoniya, G.D. Zasukhina

引用次数: 1

RecA-like activity in mammalian cell extracts of different origin 不同来源的哺乳动物细胞提取物中reca样活性

Mutation Research/DNA Repair Reports Pub Date : 1987-11-01 DOI: 10.1016/0167-8817(87)90021-6

Kerstin Kenne , Siv Ljungquist

引用次数: 15

The fate of 8-methoxypsoralen-photoinduced DNA interstrand crosslinks in Fanconi's anemia cells of defined genetic complementation groups 8-甲氧基补骨脂素光诱导的DNA链间交联在范可尼贫血细胞中的命运

Mutation Research/DNA Repair Reports Pub Date : 1987-11-01 DOI: 10.1016/0167-8817(87)90026-5

D. Papadopoulo, D. Averbeck, E. Moustacchi

{"title":"The fate of 8-methoxypsoralen-photoinduced DNA interstrand crosslinks in Fanconi's anemia cells of defined genetic complementation groups","authors":"D. Papadopoulo, D. Averbeck, E. Moustacchi","doi":"10.1016/0167-8817(87)90026-5","DOIUrl":"10.1016/0167-8817(87)90026-5","url":null,"abstract":"<div>The fate of 8-methoxypsoralen (8-MOP)-photoinduced DNA interstrand crosslinks was followed by alkaline elution in Fanconi's anemia (FA) fibroblasts belonging to complementation groups A (FA 150 and FA 402) and B (FA 145) in comparison to a normal (1 BR/3) and a heterozygote (F 311) cell line. Clonogenic cell survival to 8-MOP photoaddition was established in parallel for all cell lines. In comparison to normal cells, group A FA cells demonstrated a higher photosensitivity than group B cells (sensitivity index 2.3 and 1.5, respectively), the heterozygote cell line being only slightly more sensitive.FA cells from both groups A and B demonstrated an incision capacity of crosslinks, the kinetics and extent of which being, however, different from that of normal or heterozygote cells. The incision is slower in FA cells and, at 24 h of post-treatment incubation, the amount of crosslinks incised is clearly lower than that observed in normal cells for group A cells, whereas in group B cells incision approaches the level of normal cells. These results correlate with survival as well as with rates of DNA semi-conservative synthesis after 8-MOP photoaddition.</div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(87)90026-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14790610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 65

Differential sensitivities of transformed and untransformed murine cell lines to DNA cross-linking agents relative to repair of O6-methylguanine 转化和未转化小鼠细胞系对DNA交联剂与o6 -甲基鸟嘌呤修复的差异敏感性

Mutation Research/DNA Repair Reports Pub Date : 1987-11-01 DOI: 10.1016/0167-8817(87)90020-4

Takashi Yagi , Rufus S. Day III

引用次数: 9

Induction and repair of closely opposed pyrimidine dimers in Saccharomyces cerevisiae 密切对立嘧啶二聚体在酿酒酵母中的诱导与修复

Mutation Research/DNA Repair Reports Pub Date : 1987-11-01 DOI: 10.1016/0167-8817(87)90017-4

Richard J. Reynolds

引用次数: 13

Hypersensitivity of Bloom's syndrome fibroblasts to N-ethyl-N-nitrosourea 布卢姆综合征成纤维细胞对n -乙基-n -亚硝基脲的超敏反应

Mutation Research/DNA Repair Reports Pub Date : 1987-09-01 DOI: 10.1016/0167-8817(87)90071-X

Takayuki Kurihara , Masao Inoue , Kouichi Tatsumi

引用次数: 21