Hypersensitivity of Bloom's syndrome fibroblasts to N-ethyl-N-nitrosourea

Takayuki Kurihara , Masao Inoue , Kouichi Tatsumi
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引用次数: 21

Abstract

Fibroblast cells from two Japanese patients with Bloom's syndrome (BS) and normal donors were studied for the inactivation of colony-forming ability and the induction of sister-chromatid exchanges (SCEs) after N-ethyl-N-nitrosourea (ENU) treatment. The reduction of ENU-induced SCEs as a function of post-treatment incubation time was also compared between BS and normal fibroblasts. BS cells were approximately 4 times more sensitive than normal cells to the lethal effect of ENU and remarkably hypersensitive to the SCE induction by ENU. The post-treatment incubation of ENU-treated normal cells in the fresh medium resulted in a time-dependent decrease of the SCE level until 6 h after which time the SCE level remained the plateau of about 50% of the initial level. In contrast, the ENU-induced SCEs in BS cells decreased much more slowly with post-treatment incubation time and its half life was 24 h. These results collectively support the view that BS cells may be defective in the rapid repair of certain type(s) of DNA damages induced by ENU.

布卢姆综合征成纤维细胞对n -乙基-n -亚硝基脲的超敏反应
研究了两名日本布卢姆综合征(BS)患者和正常供体的成纤维细胞在n -乙基-n -亚硝基脲(ENU)处理后集落形成能力的失活和姐妹染色单体交换(ses)的诱导。我们还比较了enu诱导的成纤维细胞的减少与处理后培养时间的关系。BS细胞对ENU致死效应的敏感性约为正常细胞的4倍,对ENU诱导的SCE异常敏感。enu处理后的正常细胞在新鲜培养基中孵育后,SCE水平呈时间依赖性下降,直到6小时后,SCE水平保持在初始水平的50%左右的平台上。相比之下,ENU诱导的BS细胞中sce随着处理后孵育时间的延长而下降得更慢,其半衰期为24 h。这些结果共同支持了BS细胞在ENU诱导的某些类型DNA损伤的快速修复中可能存在缺陷的观点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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