Mutation Research Letters最新文献_第7页

Vitamin E prevents exercise-induced DNA damage 维生素E可以防止运动引起的DNA损伤

Mutation Research Letters Pub Date : 1995-04-01 DOI: 10.1016/0165-7992(95)90035-7

Andreas Hartmann , Andreas M. Nieβ , Martina Grünert-Fuchs , Bertram Poch , Günter Speit

{"title":"Vitamin E prevents exercise-induced DNA damage","authors":"Andreas Hartmann , Andreas M. Nieβ , Martina Grünert-Fuchs , Bertram Poch , Günter Speit","doi":"10.1016/0165-7992(95)90035-7","DOIUrl":"10.1016/0165-7992(95)90035-7","url":null,"abstract":"<div>The single cell gel test (SCG test or comet assay) was used to study DNA damage in peripheral white blood cells (WBC) of humans after a single bout of exhaustive exercise and the effect of vitamin supplementation. Human subjects were asked to run on a treadmill until exhaustion and blood samples were taken before and 24 h after the run. A clear increase in DNA strand breakage was observed in the 24-h sample of all probands. A short-term application of multivitamin pills or vitamin E (3 × 800 mg) resulted in a significantly smaller increase of DNA effects in WBC of some probands. When the volunteers were given a supplement of vitamin E (1200 mg daily) for 14 days prior to run, exercise-induced DNA damage was clearly reduced in all probands. In four out of five subjects, vitamin supplementation completely prevented the induction of DNA damage after exhaustive exercise. Intake of vitamin E for 14 days led to a clear increase in vitamin E serum concentrations. Malondialdehyde (MDA), a marker of lipid peroxidation, was measured in the serum of probands in tests with and without vitamin supplementation for 14 days. MDA concentrations were significantly decreased following vitamin E supplementation but not significantly changed 15 min and 24 h after a run. Our results demonstrate that vitamin E prevents exercise-induced DNA damage and indicate that DNa breakage occurs in WBC after exhaustive exercise as a consequence of oxidative stress.</div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"346 4","pages":"Pages 195-202"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90035-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18756560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 179

Chromosome aberrations in peripheral blood lymphocytes from control individuals 对照组外周血淋巴细胞的染色体畸变

Mutation Research Letters Pub Date : 1995-04-01 DOI: 10.1016/0165-7992(95)90034-9

Vilena Kašuba, Karmela Šentija, Vera Garaj-Vrhova, Aleksandra Fučić

引用次数: 37

Preferential binding of cisplatin to mitochondrial DNA of Chinese hamster ovary cells 顺铂与中国仓鼠卵巢细胞线粒体DNA的优先结合

Mutation Research Letters Pub Date : 1995-04-01 DOI: 10.1016/0165-7992(95)90039-X

Ofelia A. Olivero , Cristina Semino , Ahmed Kassim , Daniel M. Lopez-Larraza , Miriam C. Poirier

{"title":"Preferential binding of cisplatin to mitochondrial DNA of Chinese hamster ovary cells","authors":"Ofelia A. Olivero , Cristina Semino , Ahmed Kassim , Daniel M. Lopez-Larraza , Miriam C. Poirier","doi":"10.1016/0165-7992(95)90039-X","DOIUrl":"10.1016/0165-7992(95)90039-X","url":null,"abstract":"<div>Some chemical carcinogens localize preferentially in mitochondrial DNA (mtDNA) when compared with genomic DNA (gDNA). Here we compare the ability of cisplatin (cis-diamminedichloroplatimum[II]) to induce DNA adducts in both genomic and mtDNA of Chinese hamster ovary (CHO) cells in culture. Cytotoxicity was examined by cell survival 4, 8 and 24 h afer exposure to 50 μM cisplatin. Cisplatin-DNA adducts were measured in DNA from nuclear and mitochondrial fractions by dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA), a sensitive competitive microtiter-based immunoassay utilizing antiserum elicited against cisplatin-modified DNA. An additional comparison of cisplatin-DNA binding in both compartments was performed by immunoelectron microscopy using the cisplatin-DNA antiserum and colloidal gold. DELFIA analysis of cisplatin-DNA adducts in gDNA and mtDNA showed a six-fold higher incorporation of drug into mtDNA as compared to gDNA. Morphometric studies of colloidal gold distribution in photomicrographs of CHO cells showed mtDNA to contain a four-fold higher concentration of cisplatin as compared to nuclear DNA. Therefore, both methods demonstrated a preferential binding of cisplatin to mtDNA versus gDNA.</div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"346 4","pages":"Pages 221-230"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90039-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18754532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 68

Effect of dimethylthiourea on chromium (VI)-induced DNA single-strand breaks in Chinese hamster V-79 cells 二甲基硫脲对铬诱导的中国仓鼠V-79细胞DNA单链断裂的影响

Mutation Research Letters Pub Date : 1995-04-01 DOI: 10.1016/0165-7992(95)90041-1

Shunji Ueno , Masayasu Sugiyama , Nobuyuki Susa , Yoshinori Furukawa

{"title":"Effect of dimethylthiourea on chromium (VI)-induced DNA single-strand breaks in Chinese hamster V-79 cells","authors":"Shunji Ueno , Masayasu Sugiyama , Nobuyuki Susa , Yoshinori Furukawa","doi":"10.1016/0165-7992(95)90041-1","DOIUrl":"10.1016/0165-7992(95)90041-1","url":null,"abstract":"<div>The effect of 1,3-dimethyl-2-thiourea (DMTU), a specific hydroxyl radical scavenger on chromate-induced DNA breaks, was studied using Chinese hamster V-79 cells. Incubation of cells with Na2CrO4 plus DMTU resulted in a small but significant decrease in cellular levels of the metal-caused DNA single-strand breaks. Electron spin resonance studies showed that DMTU did not affect the formation of chromium (V) complexes either in cells or in the reaction of Na2CrO4 with reduced glutathione in vitro, however, DMTU suppressed the generation of hydroxyl radicals in the reaction of hydrogen peroxide and chromium (V) in vitro. Thus, Na2CrO4-induced DNA breaks were inhibited by DMTU, possibly due to its ability to scavenge hydroxyl radicals. These and other previous studies indicated that the formation of hydroxyl radicals contribute to the induction of DNA breaks by Na2CrO4 in intact cells, but presumably is not the only mechanism involved.</div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"346 4","pages":"Pages 247-253"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90041-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18754534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

SNG1 — a new gene involved in nitrosoguanidine resistance in Saccharomyces cerevisiae 酿酒酵母耐亚硝基胍新基因SNG1

Mutation Research Letters Pub Date : 1995-04-01 DOI: 10.1016/0165-7992(95)90037-3

Martin Grey , Claus T. Pich , Eckard Haase , Martin Brendel

{"title":"SNG1 — a new gene involved in nitrosoguanidine resistance in Saccharomyces cerevisiae","authors":"Martin Grey , Claus T. Pich , Eckard Haase , Martin Brendel","doi":"10.1016/0165-7992(95)90037-3","DOIUrl":"10.1016/0165-7992(95)90037-3","url":null,"abstract":"<div>We have molecularly characterized the SNG1 gene that confers hyper-resistance to the mutagen N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) in Saccharomyces cerevisiae when overexpressed on a multi-copy plasmid. This hyper-resistance to MNNG is not due to depletion of glutathione pools since multi-copy SNG1 containing yeast transformants contain at least wild type levels of glutathione; DNA repair seems unaffected in these transformants as the multi-copy SNG1-mediated MNNG hyper-resistance is also seen in DNA repair mutants belonging to each of the three epistasis groups of yeast repair mutants. It could be shown that SNG1 is not under control of the YAP1 encoded transcription activator that controls expression of at least two genes involved in MNNG metabolism in yeast, sng1 null mutants are viable but exhibit only slight sensitivity to MNNG, indicating that SNG1 does not encode a protein involved in a major detoxification step of this mutagen. Sequencing of the HYR-mediating passenger DNA revealed that SNG1 encodes a 547 aa polypeptide containing seven transmembrane-spanning regions that may be membrane-bound. Comparison of the DNA sequence with established gene databanks revealed that SNG1 is a novel yeast gene.</div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"346 4","pages":"Pages 207-214"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90037-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18756562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Genotoxic effects of deltamethrin in the mouse bone marrow micronucleus assay 溴氰菊酯对小鼠骨髓微核试验的遗传毒性作用

Mutation Research Letters Pub Date : 1995-04-01 DOI: 10.1016/0165-7992(95)90036-5

G. Gandhi , J.B. Chowdhury , P.K. Sareen , V.P.S. Dhillon

引用次数: 27

Increased mutability by oxidative stress in OxyR-deficient Escherichia coli and Salmonella typhimurium cells: clonal occurrence of the mutants during growth on nonselective media 氧化应激增加缺氧大肠杆菌和鼠伤寒沙门氏菌细胞的易变性:突变体在非选择性培养基上生长时克隆发生

Mutation Research Letters Pub Date : 1995-04-01 DOI: 10.1016/0165-7992(95)90038-1

Manuel Blanco, Guadalupe Herrera, Amparo Urios

{"title":"Increased mutability by oxidative stress in OxyR-deficient Escherichia coli and Salmonella typhimurium cells: clonal occurrence of the mutants during growth on nonselective media","authors":"Manuel Blanco, Guadalupe Herrera, Amparo Urios","doi":"10.1016/0165-7992(95)90038-1","DOIUrl":"10.1016/0165-7992(95)90038-1","url":null,"abstract":"<div>Escherichia coli and Salmonella typhimurium strains decifient in the OxyR-regulated adaptive response to oxidative stress were used to study the mode in which spontaneous SOS-dependent mutations are generated in a distressed bacterial population. When assayed on supplemented selective medium, the E. coli strain IC3821 (trpE65), carrying the ΔoxyR30 mutation and containing the plasmid pRW144 (mucA/B), showed a frequency of spontaneous Trp+ revertants similar to that of the oxyR+ control. Instead, the IC3821 strain exhibited an enhancement in the clonal occurrence of spontaneous revertants arising at random during growth on a nonselective medium. A similar enhancement was observed for the S. typhimurium strain TA4125 (hisG428 ΔoxyR2). The mutator effect observed in oxyR− cells would be induced by an increased background of reactive oxygen species; it provides a model for studying the mutability of a cell population constantly exposed to mutation-inducing agents. In the IC3821 strain, revertants were induced by f-butyl hydroperoxide with higher efficiency than in oxyR+. We suggest that strain IC3821 could be useful for the detection of SOS-dependent mutagenesis induced by chemical oxidants.</div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"346 4","pages":"Pages 215-220"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90038-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18754531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Contents volume 346 (1995) 目录第346卷（1995年）

Mutation Research Letters Pub Date : 1995-04-01 DOI: 10.1016/0165-7992(95)90042-X

引用次数: 0

Genotoxic effects of the chlorinated hydroxyfuranones 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone and 3,4-dichloro-5-hydroxy-2[5H]-furanone in Tradescantia micronucleus assays 氯代羟基呋喃酮3-氯-4-(二氯甲基)-5-羟基-2[5H]-呋喃酮和3,4-二氯-5-羟基-2[5H]-呋喃酮在Tradescantia微核试验中的遗传毒性作用

Mutation Research Letters Pub Date : 1995-03-01 DOI: 10.1016/0165-7992(95)90051-9

Christoph Helma , Leif Kronberg , Te-Hsiu Ma , Siegfried Knasmüller

引用次数: 18

Cytological effects of 50 Hz electromagnetic fields on human lymphocytes in vitro 50 Hz电磁场对体外人淋巴细胞的细胞学影响

Mutation Research Letters Pub Date : 1995-03-01 DOI: 10.1016/0165-7992(95)90047-0

A. Antonopoulos , Baochu Yang , A. Stamm , W.-D. Heller , G. Obe

引用次数: 75