Micron (1969)Pub Date : 1982-01-01DOI: 10.1016/0047-7206(82)90029-2
Margaret R. Mott, R.J. Hill, E.J. Burnett
{"title":"Immunolocalization of chromosomal proteins on polytene chromosomes of Drosophila","authors":"Margaret R. Mott, R.J. Hill, E.J. Burnett","doi":"10.1016/0047-7206(82)90029-2","DOIUrl":"10.1016/0047-7206(82)90029-2","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"13 3","pages":"Pages 301-302"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(82)90029-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53855325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1982-01-01DOI: 10.1016/0047-7206(82)90050-4
S.P. Flaherty, W.G. Breed
{"title":"The structure of the hooks on the sperm head of the plains mouse Pseudomys australis","authors":"S.P. Flaherty, W.G. Breed","doi":"10.1016/0047-7206(82)90050-4","DOIUrl":"10.1016/0047-7206(82)90050-4","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"13 3","pages":"Pages 343-344"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(82)90050-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53855359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1982-01-01DOI: 10.1016/0047-7206(82)90081-4
James R. Harris
{"title":"The production of paracrystalline two-dimensional monolayers of purified protein molecules","authors":"James R. Harris","doi":"10.1016/0047-7206(82)90081-4","DOIUrl":"10.1016/0047-7206(82)90081-4","url":null,"abstract":"<div><p>The application of the negative staining-carbon film technique to a range of different high molecular weight protein molecules is described. The influence of symmetrical and asymmetrical quaternary conformation of the protein on the success as previously described fo icosahedral virus particles by Horne and his colleagues, in much the same manner as previously described for icosahedral virus particles by Horne and his colleagues, in readily producing ordered two-dimensional monolayer arrays. Nevertheless, random and ordered multi-layer regions of apoferritin are also encountered. The other proteins studied, namely, human erythrocyte cylindrin and torin, the <em>Bordetella pertussis</em> ‘22S antigen’ and <em>Escherichia coli</em> glutamine synthetase, all of which have asymmetrical quaternary conformations based on the hollow cylinder model, produce ordered arrays to a lesser extent. Factors such as the preferential orientation of the protein on the mica and carbon film surface, the inter-molecular interactions which may tend to assist or prevent ordered monolayer formation, and the protein concentration will all influence the success achieved. The problems encountered can be approached by slight variation of the technique with respect to the negative stains used, their pH and concentration, the temperature and the length of time during which the protein solution dries on to the mica surface.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"13 2","pages":"Pages 147-168"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(82)90081-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53858273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1982-01-01DOI: 10.1016/0047-7206(82)90083-8
J. Burgess, P.J. Linstead
{"title":"Congo red inhibition of wall regeneration by physcomitrella protoplasts: Structural aspects","authors":"J. Burgess, P.J. Linstead","doi":"10.1016/0047-7206(82)90083-8","DOIUrl":"10.1016/0047-7206(82)90083-8","url":null,"abstract":"<div><p>The effect of the dye congo red on the formation of a fibrous wall at the surface of protoplasts of the moss <em>Physcomitrella patens</em> has been studied by electron microscopy. Concentrations of the dye above 10mg 1<sup>−1</sup> caused total inhibition of fibre formation judged by scanning electron microscopy. At lower concentrations, the presence of the dye results in abnormal walls being formed. These show short atypical fibres of variable thickness. Negative staining shows the presence amongst such fibres of amorphous material. When protoplasts are incubated with media containing congo red for 4hr at 23°C, about half the dye can be recovered as a pellet following high speed centrifugation of the medium. This proportion is reduced at 0°C and also by the additional presence of coumarin in the medium. The pellet is non-fibrous, but contains glucose as the only detectable sugar following hydrolysis and thin layer chromatography. The results are discussed in terms of general ideas concerning the polymerization and crystallization of structural polysaccharides.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"13 2","pages":"Pages 185-192"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(82)90083-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53858416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1982-01-01DOI: 10.1016/0047-7206(82)90012-7
H. Jaeger, K. Foger
{"title":"The application of electron microscopy to the redispersion of support noble metal catalysts","authors":"H. Jaeger, K. Foger","doi":"10.1016/0047-7206(82)90012-7","DOIUrl":"10.1016/0047-7206(82)90012-7","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"13 3","pages":"Pages 267-268"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(82)90012-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53854329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1982-01-01DOI: 10.1016/0047-7206(82)90052-8
A.T. Marshall
{"title":"Electrolyte gradients in biological transporting epithelia revealed by digital X-ray line scans of frozen-hydrated bulk specimens","authors":"A.T. Marshall","doi":"10.1016/0047-7206(82)90052-8","DOIUrl":"10.1016/0047-7206(82)90052-8","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"13 3","pages":"Pages 347-348"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(82)90052-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53855587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1982-01-01DOI: 10.1016/0047-7206(82)90062-0
Gutta I. Schoefl
{"title":"Vascular pericytes in the chorio-allantois of the chick embryo","authors":"Gutta I. Schoefl","doi":"10.1016/0047-7206(82)90062-0","DOIUrl":"10.1016/0047-7206(82)90062-0","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"13 3","pages":"Pages 367-368"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(82)90062-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53856246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}