Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90033-X
Alan Wolfenden
{"title":"Special techniques for metallographic examination of irradiated fuel pin wire wrap material from EBR-II","authors":"Alan Wolfenden","doi":"10.1016/0047-7206(83)90033-X","DOIUrl":"10.1016/0047-7206(83)90033-X","url":null,"abstract":"<div><p>Metallographic techniques have been developed for examination of wire wrap sections of neutron irradiated AISI 316 stainless steel from EBR-II. The special etching techniques used for metallographic examination of radiation and thermally induced precipitates, including sigma or chi phases and alpha ferrite, are documented. A description is given of the preparation of standard 3 mm dia TEM foils using a nickel plating technique to increase the effective size of the original 1.1–1.4 mm dia wires.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 63-68"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90033-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53860917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90037-7
Susan A. Gross, L.J.A. DiDio
{"title":"Electron microscopy of the prostate in non-pregnant and pregnant Praomys (Mastomys) Natalensis","authors":"Susan A. Gross, L.J.A. DiDio","doi":"10.1016/0047-7206(83)90037-7","DOIUrl":"10.1016/0047-7206(83)90037-7","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 79-80"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90037-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53861182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90030-4
G.D. Danilatos, R. Postle
{"title":"Design and construction of an atmospheric or environmental SEM—2","authors":"G.D. Danilatos, R. Postle","doi":"10.1016/0047-7206(83)90030-4","DOIUrl":"10.1016/0047-7206(83)90030-4","url":null,"abstract":"<div><p>This paper is a continuation of a series of reports on the design and construction of an atmospheric or environmental SEM. The work described is an extended study of the gas jet developed above the pressure limiting aperture (Bell, 1974; Lacaze <em>et al.,</em> 1977). Experiments specifically aimed to establish how the vacuum in the electron optics system was affected by the relative positioning of the objective and pressure limited aperture, as well as the pumping speeds employed, specimen chamber pressure, geometry and size of apertures, and by other means. Further, the nfluence of the jet deflectors, to control the effects of this jet on the microscope system were studied quantitatively using a specifically designed apparatus. In addition, the study of the pressure gradients below the pressure limiting aperture revealed that specimens can be placed as close as radius from the aperture and still experience an almost saturated vapour pressure environment. The results of the present study are currently being used in the design of an optimum detection configuration. A preliminary result has allowed the use of 140 μm pressure limiting aperture to observe specimens at atmospheric pressures as well as the use of low accelerating voltages (e.g. 7 kV) at TV scanning rates to record on video cassette dynamic phenomena, including wetting or recrystallizing salt solutions, etc.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 41-52"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90030-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53860601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90015-8
Takashi Okagaki, Barbara A. Clark, Leo B. Twiggs, Richard M. Condie
{"title":"Enumeration of the number of microprocesses of cultured murine T-lymphoma cells: A comparative study of two new morphometric methods","authors":"Takashi Okagaki, Barbara A. Clark, Leo B. Twiggs, Richard M. Condie","doi":"10.1016/0047-7206(83)90015-8","DOIUrl":"10.1016/0047-7206(83)90015-8","url":null,"abstract":"<div><p>Two morphometric methods, one using a transmission electron microscope (TEM), the other using a scanning electron microscope (SEM), were developed to quantitate the number of surface microprocesses, discs or pores over spherical cells. The number of the surface processes over EL-4 murine T-lymphoma cells cultured <em>in vitro</em> were measured by the two methods. The measurement using TEM yielded 511 ± 45 microprocesses per cell, whereas SEM enumerated 532 ± 25 microprocesses per cell. The method using a TEM, in effect, results in reduction of observed data, and may have an advantage over the method using a SEM when the structures of interest are numerous.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 2","pages":"Pages 135-140"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90015-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53859842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90017-1
J.R. Harris
{"title":"Electron microscopy of papain crystals","authors":"J.R. Harris","doi":"10.1016/0047-7206(83)90017-1","DOIUrl":"10.1016/0047-7206(83)90017-1","url":null,"abstract":"<div><p>Crystalline suspensions of the proteolytic enzyme papain have been studied by negative staining with sodium phosphotungstate and uranyl acetate, and by thin sectioning. The needle-like crystals of papain exhibit pronounced longitudinal and transverse periodicities and are clearly shown to be formed by the coalescence of parallel bundles of individual fibrils. Optical diffractometry performed on suitable regions of the electron micrographs containing papain crystals have been employed to produce the optical transform for subsequent image filtering. Transverse thin sections of the thicker crystals reveals a complex honeycomb organization with pronounced aqueous channels. This organization is held to be responsible for the varying images of the negatively stained crystals, which result from the varying orientations of the crystal axis relative to the electron beam. It is proposed that papain may represent another useful protein for studying electron microscopically the fibre-to-crystal transformation process, as well as for the understanding of the formation of varying crystalline states.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 2","pages":"Pages 147-164"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90017-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53860722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90039-0
Jay T. Hairston, Jong S. Yoon
{"title":"Species identification in some Hawaiian drosophila utilizing mouthparts morphology","authors":"Jay T. Hairston, Jong S. Yoon","doi":"10.1016/0047-7206(83)90039-0","DOIUrl":"10.1016/0047-7206(83)90039-0","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 83-84"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90039-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53861300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90040-7
William C. Hamlett , John P. Wourms
{"title":"Electron microscopy of a shark yolk sac placenta prior to implantation and differentiation","authors":"William C. Hamlett , John P. Wourms","doi":"10.1016/0047-7206(83)90040-7","DOIUrl":"10.1016/0047-7206(83)90040-7","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 85-86"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90040-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53861311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90038-9
Gunning W.T., Harris J.H.
{"title":"Intranuclear tubular inclusions and cytoplasmic bodies in a patient with chronic interstitial fibrosis","authors":"Gunning W.T., Harris J.H.","doi":"10.1016/0047-7206(83)90038-9","DOIUrl":"10.1016/0047-7206(83)90038-9","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 81-82"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90038-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53861223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}