Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90038-9
Gunning W.T., Harris J.H.
{"title":"Intranuclear tubular inclusions and cytoplasmic bodies in a patient with chronic interstitial fibrosis","authors":"Gunning W.T., Harris J.H.","doi":"10.1016/0047-7206(83)90038-9","DOIUrl":"10.1016/0047-7206(83)90038-9","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 81-82"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90038-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53861223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90035-3
Richard E. Crang , Susan G. Robinson , Reginald D. Noble , Leon S. Dochinger
{"title":"Acid fog effects on yellow poplar leaf morphology","authors":"Richard E. Crang , Susan G. Robinson , Reginald D. Noble , Leon S. Dochinger","doi":"10.1016/0047-7206(83)90035-3","DOIUrl":"10.1016/0047-7206(83)90035-3","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 75-76"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90035-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53861035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90013-4
Thomas von Zglinicki, Stephan Heymann
{"title":"Schlieren optical imaging of weak objects","authors":"Thomas von Zglinicki, Stephan Heymann","doi":"10.1016/0047-7206(83)90013-4","DOIUrl":"10.1016/0047-7206(83)90013-4","url":null,"abstract":"<div><p>It is shown that with the aid of a schlieren optical mode described in this paper in real electron microscopes, the amplitude contrast of weak objects can be enhanced up to two-fold in comparison with the bright-field mode, if a part of the unscattered beam strikes the objective aperture. This remains true also in the case of thicker objects imaged (for example, unstained sections of biological matter). Futhermore, evidence is given for the existence of an optimum schlieren image in the transition range between strict schlieren optics and conventional bright-field microscopy. Such images are characterized by high amplitude contrast without significant distortion. Theory and experiment are shown to be in good agreement. As an application, unstained nucleosomes have been investigated schlieren-optically. Model calculations show that the ‘hole’ in their centre, which has been demonstrated from dark field images, is probably not due to a structural void (cavity), but is a consequence of the lower density of the protein core as compared with DNA.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 2","pages":"Pages 109-118"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90013-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53860292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90027-4
B. Wells , R.W. Horne , Barbara M. Lund , N.R. King
{"title":"The ultrastructure of Pseudomonas aven ae— I. Paracrystalline surface layer and extracellular material","authors":"B. Wells , R.W. Horne , Barbara M. Lund , N.R. King","doi":"10.1016/0047-7206(83)90027-4","DOIUrl":"10.1016/0047-7206(83)90027-4","url":null,"abstract":"<div><p>The general morphological features of the Gram-negative bacterium <em>Pseudomonas avenae</em> have been studied by electron microscopy. Electron micrographs from negatively stained specimens show the outermost surface layer to be composed of subunits arranged in a crystalline tetragonal array, with an average lattice spacing of 6.7 nm and unit cell of 9.7 nm when determined by image processing methods. Experiments designed to show the effects of various negative stains on the bacterial cell wall morphology suggest that the ‘convoluted’ or ‘smooth’ appearance of the wall material can be influenced by different stains when mixed with cells in liquid suspension.</p><p>Various morphological products assembled as extracellular structures from subunits apparently of about the same size as those forming the cell surface crystalline lattice were observed in specimens prepared from a solid growth medium. These extracellular products ranged from tube-like assemblies with considerable variation in length, to forms which consisted of slender filaments in a coiled configuration. The axial periodicity of some of these products was 6.7 nm.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 11-28"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90027-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53860472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90022-5
{"title":"British biophysical society low temperature transmission electron microscopy","authors":"","doi":"10.1016/0047-7206(83)90022-5","DOIUrl":"https://doi.org/10.1016/0047-7206(83)90022-5","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 2","pages":"Page 190"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90022-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92026509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A digital processing method for structural analysis of atom clusters from high resolution electron micrographs","authors":"Koichi Kanaya, Kengo Takamiya, Masayuki Shino, Chiharu Shinohara","doi":"10.1016/0047-7206(83)90014-6","DOIUrl":"10.1016/0047-7206(83)90014-6","url":null,"abstract":"<div><p>The electron image contrast of tungsten atom clusters changes remarkably depending on the spatial frequency, scattering factor phase, lens aberration and defocusing, but it is confirmed that the image appears in negative contrast and satisfies an anastigmatic condition when recorded under optimum focus.</p><p>When based on a cluster model, the spatial frequencies can be divided into individual atom clusters by successive band pass filters. An electron micrograph taken at the optimum focus condition is selected from a defocus series of micrographs and is used for digital processing. In order to measure the exact size of the clusters, the enhancement of filtered images by their gradient images to extract edge information and the subsequent superimposition of both images are derived.</p><p>Based on Fourier analysis, it is proposed that tungsten films consist of double cluster layers which correspond to a thickness consisting of 3–5 single atoms. In addition, their three-dimensional localization can be determined more precisely than by earlier computer-aided reconstruction methods.</p><p>The cluster models are in good agreement with the processed data suggesting that the amorphous phase is a distinct phase and not microcrystalline.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 2","pages":"Pages 119-134"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90014-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53860308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90034-1
J.N. Brown
{"title":"An improved apparatus for staining large numbers of electron microscope ultrathin sections simultaneously","authors":"J.N. Brown","doi":"10.1016/0047-7206(83)90034-1","DOIUrl":"10.1016/0047-7206(83)90034-1","url":null,"abstract":"<div><p>This paper describes a simple efficient device which permits the simultaneous staining and washing of a large number of electron microscope ultrathin sections.</p></div>","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 69-71"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90034-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53861003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micron (1969)Pub Date : 1983-01-01DOI: 10.1016/0047-7206(83)90046-8
Charles W. Noble, William C. Hamlett, Patricia McCann, Dennis E. Morse
{"title":"Morphogenesis of chordae tendineae in the avian embryo","authors":"Charles W. Noble, William C. Hamlett, Patricia McCann, Dennis E. Morse","doi":"10.1016/0047-7206(83)90046-8","DOIUrl":"10.1016/0047-7206(83)90046-8","url":null,"abstract":"","PeriodicalId":100924,"journal":{"name":"Micron (1969)","volume":"14 1","pages":"Pages 97-98"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0047-7206(83)90046-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53861823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}