Journal of Pharmaceutical and Biomedical Analysis Open最新文献_第8页

Efficient screening of anti-idiotype DNA aptamers that bind specifically to trastuzumab for bioanalytical applications 有效筛选抗独特型DNA适体，特异性结合曲妥珠单抗用于生物分析应用

Journal of Pharmaceutical and Biomedical Analysis Open Pub Date : 2023-06-01 DOI: 10.1016/j.jpbao.2023.100006

Kenichiro Todoroki , Jiaxing Tong , Moe Aoki , Nao Kobayashi , Ryota Isobe , Hiroyuki Tasaki , Tomohiro Yamada , Aogu Furusho , Eiji Sugiyama , Hajime Mizuno , Hideki Hayashi , Toshimasa Toyo’oka

{"title":"Efficient screening of anti-idiotype DNA aptamers that bind specifically to trastuzumab for bioanalytical applications","authors":"Kenichiro Todoroki , Jiaxing Tong , Moe Aoki , Nao Kobayashi , Ryota Isobe , Hiroyuki Tasaki , Tomohiro Yamada , Aogu Furusho , Eiji Sugiyama , Hajime Mizuno , Hideki Hayashi , Toshimasa Toyo’oka","doi":"10.1016/j.jpbao.2023.100006","DOIUrl":"https://doi.org/10.1016/j.jpbao.2023.100006","url":null,"abstract":"<div><p>We have developed a novel aptamer discovery method that rapidly and efficiently yields anti-idiotypic DNA aptamers for trastuzumab using fast protein liquid chromatography (FPLC) separation and large amounts of DNA. The use of large amounts of oligo DNA allowed us to obtain more aptamer candidates without PCR amplification within only two days. Quartz crystal microbalance (QCM) measurements confirmed that the obtained anti-trastuzumab aptamer had a high affinity with a <em>K</em><sub>D</sub> of 120.0 nM and 97.7 nM at pH 6.0 and 7.4, respectively. Molecular docking simulations suggested that this sequence has high specificity and binding affinity to multiple sites in the complementarity-determining region of trastuzumab. The <em>K</em><sub>D</sub> increased to 11.4 nM due to avidity expression after dense immobilization in the QCM sensor cell, indicating that this anti-trastuzumab aptamer is applicable as a capture molecule in the ligand binding assay.</p></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"1 ","pages":"Article 100006"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49883934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Combination quantitative 1H NMR and chemometric approaches for the assessment of quality control in commercially available products of red fruit (Pandanus conoidues, Lam.) oil 结合定量1H NMR和化学计量学方法评价市售红果油产品的质量控制

Journal of Pharmaceutical and Biomedical Analysis Open Pub Date : 2023-06-01 DOI: 10.1016/j.jpbao.2023.100010

Liling Triyasmono , Ulrike Holzgrabe

引用次数: 1

Recent applications of the derivatization techniques in capillary electrophoresis 衍生化技术在毛细管电泳中的最新应用

Journal of Pharmaceutical and Biomedical Analysis Open Pub Date : 2023-06-01 DOI: 10.1016/j.jpbao.2023.100003

Roberto Gotti , Benedetta Pasquini , Serena Orlandini , Sandra Furlanetto

{"title":"Recent applications of the derivatization techniques in capillary electrophoresis","authors":"Roberto Gotti , Benedetta Pasquini , Serena Orlandini , Sandra Furlanetto","doi":"10.1016/j.jpbao.2023.100003","DOIUrl":"https://doi.org/10.1016/j.jpbao.2023.100003","url":null,"abstract":"<div><p>The main reasons for performing derivatization in capillary electrophoresis are largely the same as for liquid chromatography, however there are specific aspects in electrokinetic separations where derivatization plays specific roles. The review is focused on the articles published in the past 5 years with the aim to highlight this unicity. Derivatization is mainly applied to improve the inherent low sensitivity of capillary electrophoresis when optical detection is used and the introduction of originally developed derivatization approaches have been addressed mainly to the detection by laser-induced fluorescence. A further peculiarity concerns the development of automatized as well as in-capillary derivatization that can be performed using the commercially available instrumentation. The majority of the methods considered deal with the derivatization of amine group in small molecules (in particular, amino acids) as well as in proteins and peptides. Applications are also addressed to chiral analysis and for trapping unstable and reactive small molecules and inorganic ions. The analysis of proteins and saccharides in glycomics, have been covered in dedicated sections.</p></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"1 ","pages":"Article 100003"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49883905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Determination of carbamazepine and its main metabolite in human hair by capillary electrophoresis and liquid chromatography techniques, both coupled with mass spectrometry 毛细管电泳和液相色谱联用质谱法测定头发中卡马西平及其主要代谢物

Journal of Pharmaceutical and Biomedical Analysis Open Pub Date : 2023-06-01 DOI: 10.1016/j.jpbao.2023.100009

Aneta Woźniakiewicz, Renata Wietecha-Posłuszny

{"title":"Determination of carbamazepine and its main metabolite in human hair by capillary electrophoresis and liquid chromatography techniques, both coupled with mass spectrometry","authors":"Aneta Woźniakiewicz, Renata Wietecha-Posłuszny","doi":"10.1016/j.jpbao.2023.100009","DOIUrl":"https://doi.org/10.1016/j.jpbao.2023.100009","url":null,"abstract":"<div><p>The aim of the research was to determine carbamazepine (CBZ) and its main metabolite 10,11-epoxy-10,11-dihydro-carbamazepine (CBZ-E) in human hair using the capillary electrophoresis (CE) system coupled with mass spectrometry detection (MS) and to compare the obtained results with the liquid chromatography (LC) technique, also coupled with the MS detector. Hair samples were prepared using microwave-assisted extraction (MAE) at 60 °C for 10 min in an alkaline solution (pH = 10) with ethyl acetate as the extraction solvent. In the frame of this study, the procedure for the separation of CBZ and CBZ-E using the CE-MS technique was developed. The best results were achieved using 10 mM ammonium acetate (pH=6.8) as the background electrolyte (BGE), after filling the capillary with 1% highly sulfonated β-cyclodextrin (HSβCD) in 10 mM ammonium acetate. Then, the validation parameters of the MAE/CE-MS and MAE/LC-MS methods such as: limit of detection (for CBZ are: 0.36 and 0.22 ng/mg, respectively; for CBZ-E 0.38 and 0.17 ng/mg, respectively), limit of quantification (for CBZ are: 0.86 and 0.72 ng/mg, respectively; for CBZ-E 0.94 and 0.56 ng/mg, respectively), precision (6.91–14.5% and 2.16–15.6%, respectively), recovery (87.7–102.7% and 88.9–105.5%, respectively), and matrix effect (99.5–111.0% and 98.9–115.1%, respectively) were defined and compared. Finally, the validated methods were applied to identify and quantify carbamazepine and its metabolite in hair in patients who received CBZ for medical reasons.</p></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"1 ","pages":"Article 100009"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49883936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison of the use of 6-thioguanine riboside versus 6-thioguanine as calibration standard to monitor 6-thioguanine nucleotides in red blood cells 使用6-硫鸟嘌呤核苷与6-硫鸟嘌呤作为校准标准物监测红细胞中6-硫鸟嘌呤核苷酸的比较

Journal of Pharmaceutical and Biomedical Analysis Open Pub Date : 2023-06-01 DOI: 10.1016/j.jpbao.2023.100007

Roselyne Boulieu , Antoine Tourlonias , Magali Larger

{"title":"Comparison of the use of 6-thioguanine riboside versus 6-thioguanine as calibration standard to monitor 6-thioguanine nucleotides in red blood cells","authors":"Roselyne Boulieu , Antoine Tourlonias , Magali Larger","doi":"10.1016/j.jpbao.2023.100007","DOIUrl":"https://doi.org/10.1016/j.jpbao.2023.100007","url":null,"abstract":"<div><p>The analytical methods reported to evaluate 6-Thioguanine nucleotides (6-TGNs) level in red blood cells (RBC) are based on the conversion of 6-TGNs to 6-Thioguanine (6-TG) using 6-thioguanine as calibration standard.</p><p>Using the LC-DAD method previously reported by Dervieux and Boulieu in 1998 to determine 6-TGNs, we evaluated the use of 6-Thioguanine Riboside (6-TGR) as standard instead of the base 6-TG for the monitoring of 6-TGNs in RBC from patients with IBD.</p><p>Our results show that 6-TGN values measured in RBC from 30 patients were significantly (p < 0,00001) higher when 6-TGR was used as calibration standard compared to 6-TG. The difference observed may be explained by the presence of ribose in the chemical structure of 6-TGR contrary to 6-TG. This difference in 6-TGN values was also observed in external quality control assay using 6-TGR versus 6-TG calibration standard.</p><p>The use of the nucleoside 6-TGR as calibrator constitutes a better reflection of the chemical reaction which occurs in RBC compared to 6-TG. This preliminary observation suggests that the choice of calibration standard to monitor 6-TGNs may have a significant impact on the values measured in patients and laboratory should be aware of this potential pitfall.</p></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"1 ","pages":"Article 100007"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49885071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0