Food Physics最新文献

{"title":"Simulated construction of plant-based fish meat with composite structure via dual-nozzle extrusion 3D printing","authors":"Jie Li ,&nbsp;Haohao Hu ,&nbsp;Ruihao Niu ,&nbsp;Qingqing Zhu ,&nbsp;Siyu Yao ,&nbsp;Jianwei Zhou ,&nbsp;Donghong Liu ,&nbsp;Enbo Xu","doi":"10.1016/j.foodp.2024.100028","DOIUrl":"10.1016/j.foodp.2024.100028","url":null,"abstract":"<div><p>Three-dimensional (3D)-printed fish analogs are gradually nearing the ability to mimic real fish meat in response to personal demand, supply pressure, food safety, and environmental concerns. However, the use of 3D food printing to simulate the composite structure of real meat tissue remains a challenge. In this study, we used dual-nozzle 3D printing technology to construct plant-based yellow croaker tissue analogs by soy protein isolate–xanthan gum-starch complex (as simulated muscle ink) and nanostarch-carrageenan emulsion gel (as simulated fat ink). We successfully prepared 3D-printed fish meat with a high simulation composite structure by constructing a muscle/fat biphasic 3D model and optimizing the printing process. The texture, moisture distribution, and nutrient content of the simulated fish meat were analyzed and compared with real yellow croaker meat, demonstrating that 3D-printed plant-based yellow croaker flesh with a composite structure had a good simulation quality.</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"2 ","pages":"Article 100028"},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950069924000227/pdfft?md5=ee27804a261fee5c8ec5944c6d8061e2&pid=1-s2.0-S2950069924000227-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancement in measurement and AI-driven predictions of maturity indices in kinnow(Citrus nobilis x Citrus deliciosa ): A comprehensive review","authors":"Sachin Ghanghas ,&nbsp;Nitin Kumar ,&nbsp;Sunil Kumar ,&nbsp;Vijay Kumar Singh","doi":"10.1016/j.foodp.2024.100026","DOIUrl":"10.1016/j.foodp.2024.100026","url":null,"abstract":"<div><p>Kinnow also known as mandarin are popular fruits worldwide for their refreshing flavor and nutritional benefits. Their quality standards vary globally due to differences in climatic conditions, agronomical practices, mandarin physiology, etc. The fruit maturity indices are region and consumer specific which make traditional methods of maturity predictions a very difficult task which become challenge for producers and researchers. This review provides state-of-art approches on maturity indices of mandarin fruit by understanding its physiological changes including their biotic, abiotic factors, physicochemical parameters and artificial intelligence integration with non-destructive technologies to predict the fruit maturity. It focuses on rapid on-field sensor and camera based systems with different algorithmic models for fruit maturity prediction. The use of AI driven advanced spectrometry, imaging techniques, real time monitoring are crucial for predicting harvest time. It also highlights significant technical challenges and identifies promising areas for future research, offering a valuable insights for growers.</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"2 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950069924000203/pdfft?md5=f0ecd57f9df129955403c1c3f2adbce8&pid=1-s2.0-S2950069924000203-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technological usage of ripe banana pulp for the development of a salad dressing","authors":"Tania Martinez ,&nbsp;Ricardo S. Aleman ,&nbsp;Franklin Delarca Ruiz ,&nbsp;Mallerly Sarmiento Madrid ,&nbsp;Jhunior Marcia ,&nbsp;Ismael Montero-Fernández ,&nbsp;Daniel Martín Vertedor ,&nbsp;Víctor Manrique-Fernández ,&nbsp;Jesús Lozano","doi":"10.1016/j.foodp.2024.100027","DOIUrl":"10.1016/j.foodp.2024.100027","url":null,"abstract":"<div><p>Oxidized banana (OB) is one of the important food wastes which contains a high potential to use in different products such as salad dressing. OB is a good ingredient in salad dressing due to its presence of antioxidants, vitamins, dietary fibers, and starch. the current study aimed to replace OB with non-oxidized banana (T₁: 0 % T₂: 25 %, T₃: 50 %, T₄: 75 %, and T₅: 100 % OB) in salad dressing formulation and focused on the effects of OB on color, sensory, physical, chemical, rheological properties, microbial growth, and consumer studies. E-nose and volatile compounds analysis by GC-MS was also performed. According to the results, L*, a*, and b* values decreased significantly (<em>P</em> &lt; 0.5) leading to a darker color of the product and effected other color evaluations (Hue, chroma, and ∆E). Viscosity was decreased significantly by the addition of the OB in the dressing formulation while pH did not change between T₅ and T₁ treatments. All sensory attributes score (9-point hedonic scale) decreased and consumers were given of fact of OB salad dressing has environmental benefits. Steady shear flow measurements, frequency sweep analysis, and the thixotropic loop of the oxidized banana indicated that adding OB to salad dressing negatively affected textural properties. The most effective attributes are, texture, and color discriminating the underlying differences among all 5 treatments according to MANOVA Wilks’ <em>P</em>-values. McNemar’s test was applied to analyze before and after consumers had been given the information about OB environmental benefits indicating that emotional attributes were changed after knowing the fact state as well as the odds ratio predicted purchase intent which increased with an increase in overall liking, pleased, flavor, and satisfied attributes. The acceptability threshold for OB in salad dressing formulation is 50 % OB. Among the identified volatile organic compounds are acetic acid, hexanoic acid, 3-methyl-1-butanol, 2-methylpropyl acetate, ethyl hexanoate, and phenylethyl acetate. The volatile organic compounds identified are 3-methyl-butanoic acid, 2-methyl-butanoic acid, and (<em>E</em>)-2-Decenal. The electronic device could discriminate these aromas, and the results obtained agree with those of the tasting panel and the volatile compounds.</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"2 ","pages":"Article 100027"},"PeriodicalIF":0.0,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950069924000215/pdfft?md5=eaa77576e72d5e472422c9c7f3238eea&pid=1-s2.0-S2950069924000215-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142122699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent trends and advancements in nanoemulsions: Production methods, functional properties, applications in food sector, safety and toxicological effects","authors":"Gurveer Kaur ,&nbsp;Chirasmita Panigrahi ,&nbsp;Swati Agarwal ,&nbsp;Anjali Khuntia ,&nbsp;Monalisa Sahoo","doi":"10.1016/j.foodp.2024.100024","DOIUrl":"10.1016/j.foodp.2024.100024","url":null,"abstract":"<div><p>Nanoemulsions - colloidal dispersions of nanoscale droplets in a continuous phase, have garnered significant attention in recent years due to their versatile properties and wide-ranging applications. This review paper presents a comprehensive overview of nanoemulsions production methods focusing on their formulation strategies, functional properties and different applications in food processing. Various low and high energy methods such as phase inversion, high pressure homogenization, ultrasonication and microfluidization etc., are reviewed to produce nanoemulsions with diverse characteristics and functionalities. Scale-up potential and practical challenges of each production method are also discussed. Various properties of nanoemulsions stability, drug solubility, bioavailability and targeted delivery are highlighted. Furthermore, their potential applications in food and beverage sectors including encapsulation of bioactive compounds, packaging, preservation and new product formulation have been elaborated along with real world examples at commercial stage. This review will contribute to providing knowledge about gastrointestinal fate of nanoemulsions, toxicity and safety by in-vitro and in-vivo testing as well as other research evidences. Regulatory aspects and public perception on use of nanoemulsions are also covered. Nevertheless, nanoemulsion presents immense potential for addressing various challenges in food industry. Continued research efforts in formulation design, ingredient selection, its compatibility with food constituents as well as the associated safety issues should be undertaken to unlock new opportunities towards scalability and commercialization.</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"1 ","pages":"Article 100024"},"PeriodicalIF":0.0,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950069924000185/pdfft?md5=9e37b40b3d026fb5947abbc53ba5dad9&pid=1-s2.0-S2950069924000185-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Cold Plasma and Thermal Treatment on the Storage Stability and Shelf-Life of Pineapple Juice: A Comprehensive Postharvest Quality Assessment","authors":"Sunil Pipliya,&nbsp;Sitesh Kumar,&nbsp;Prem Prakash Srivastav","doi":"10.1016/j.foodp.2024.100025","DOIUrl":"10.1016/j.foodp.2024.100025","url":null,"abstract":"<div><p>The primary concern regarding the pineapple juice processing industry is its insufficient stability. The goal of this investigation is to evaluate the storage stability and shelf life of cold plasma (CP) and thermally treated pineapple juice samples (S1, untreated; S2, optimized plasma-treated; S3, extreme plasma-treated; S4, thermally-treated) packed in glass (GL) and polyethylene terephthalate (PET) bottles at 5, 15, and 25°C. The color parameters increased with time and storage temperature. However, the bioactive substances decreased during storage for all the samples. Moreover, all the samples at elevated temperatures showed a higher degradation rate for bioactive compounds and color parameters. The changes in color parameter values across all samples showed zero-order kinetics, while the bioactive compounds followed a 1^st-order kinetics. The application of various packing materials and storage temperatures had a significant impact (p &lt; 0.05) on the microbiological, enzyme inactivation, and biochemical characteristics of the samples. However, there was an insignificant effect (p &gt; 0.05) on the pH, acidity, and soluble solids. CP and thermally-treated samples reduced natural microbiota below the detection limit (&lt;1-log CFU/mL). The sensory quality of S2, S3, and S4 samples remained above the acceptable limit (OA&gt;5) for 120 d at 5 ℃. The shelf-life of the optimized CP-treated juice sample packed in GL bottles was 90, 50, and 25 days at 5, 15, and 25°C, respectively, based on AA≥20<!--> <!-->mg/100<!--> <!-->mL, OA≥5, ΔE*≤12, and microbial count≤6-log CFU/mL. In conclusion, CP is an effective non-thermal method that may extend the shelf life of pineapple juice packed in GL bottles by up to 90 days in refrigerated conditions (5 °C).</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"1 ","pages":"Article 100025"},"PeriodicalIF":0.0,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950069924000197/pdfft?md5=d394b5f72b6a928e0fa387437c76858c&pid=1-s2.0-S2950069924000197-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulsed light: Innovative non-thermal technology for preservation of fruits and vegetables","authors":"Roji Waghmare ,&nbsp;Manoj Kumar ,&nbsp;Baohong Zhang ,&nbsp;Rahul Yadav ,&nbsp;Ajinath Dukare ,&nbsp;Radha ,&nbsp;Deepak Chandran ,&nbsp;Pratik Nayi ,&nbsp;Muzaffar Hasan ,&nbsp;Sangram Dhumal ,&nbsp;Trupti Dharmarao ,&nbsp;Tanu Malik ,&nbsp;Ruprekha Sarma ,&nbsp;Saurjaynee Biswas ,&nbsp;Jose M. Lorenzo","doi":"10.1016/j.foodp.2024.100022","DOIUrl":"10.1016/j.foodp.2024.100022","url":null,"abstract":"<div><p>Pulsed light (PL), a relatively new non-thermal technology can be used as a possible substitute to the conventional (thermal and chemical) technology for disinfecting a wide variety of fruits and vegetables. The short-duration light pulses, ranging from 1 to 20 flashes per second with an energy density of about 0.01–50 Jcm⁻², ensure the killing of several disease-causing microorganisms by causing minimal impact on the quality parameters in fruits and vegetables. This technology enhances safety and extends shelf-life period of fruits and vegetables. Furthermore, PL decreases or inhibits the harmful effect of conventional techniques on nutritional components. The review discusses recent research on PL's influence on nutritional and physicochemical parameters and its potential applications for extending the shelf life of fruits and vegetables. This PL technology can be successfully employed alone or in combination with other techniques for enhancing fresh produce life. PL is considered a safe, eco-friendly and non-thermal technique for shelf-life extension. For this review article, keywords such as pulsed light, fruits and vegetables, microbial safety and shelf life were selected and most relevant papers were cited. Thus, the present review article, supported by 70 references, highlights the novel potential of PL treatment and its diverse applications in the fruit and vegetable sector.</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"1 ","pages":"Article 100022"},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950069924000161/pdfft?md5=d080b0db3f171214428e29be340c5c38&pid=1-s2.0-S2950069924000161-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of magneto-induced electric field on the physicochemical, structural, and rheological properties of citrus pectin at different pH values","authors":"Ming-Yu Jin,&nbsp;Luobang Wu,&nbsp;Yahui Yu,&nbsp;Long-Qing Li,&nbsp;Xiangying Yu,&nbsp;Xiaozhen Liu,&nbsp;Fengyuan Liu,&nbsp;Yuting Li,&nbsp;Jing-Kun Yan","doi":"10.1016/j.foodp.2024.100021","DOIUrl":"10.1016/j.foodp.2024.100021","url":null,"abstract":"<div><p>This study examined the effect of magneto-induced electric field (MIEF) treatment under varying pH conditions on the physicochemical, structural, and rheological properties of citrus pectin (CP). The results indicate that as the pH increased (to 4.0, 7.0, and 10.0), the molecular weight, degree of esterification, and galacturonic acid content of CP decreased. At pH values of 4.0 and 7.0, MIEF treatment caused fractures in the side chains of CP, while at pH 10.0, the main chain broke. MIEF treatment under these conditions enhanced the steady-state fluid behavior and dynamic viscoelastic properties of CP by promoting increased entanglements of neutral sugar side chains. Overall, the combined effect of pH and MIEF led to the breakdown of the main or side chains of CP, reducing its molecular weight and thereby influencing its rheological properties. In effect, this study provides a green modification technique for CP and its application to functional foods.</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"1 ","pages":"Article 100021"},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S295006992400015X/pdfft?md5=280e2fa6a71540a9382dac2418002474&pid=1-s2.0-S295006992400015X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of phosphate salts and ultrasonication on the formation and characteristics of phosphate-chitosan microparticles","authors":"Hugo Espinosa-Andrews ,&nbsp;Jorge Alejandro Barbosa-Nuñez ,&nbsp;Moisés Martínez-Velázquez ,&nbsp;Gustavo Adolfo Castillo-Herrera ,&nbsp;José Nabor Haro-González","doi":"10.1016/j.foodp.2024.100023","DOIUrl":"10.1016/j.foodp.2024.100023","url":null,"abstract":"<div><p>The physicochemical properties of chitosan-microparticles are related to the crosslinking salts. The effects of sodium-pyrophosphate or sodium-tripolyphosphate and ultrasonication on the formation of phosphate-chitosan microparticles were evaluated. Potentiometric titration curves were used to determine the ionization degree of chitosan by employing the Henderson-Hasselbach equation and milliequivalents. Colloidal formation was validated by turbidity and conductivity at different phosphate-chitosan salt ratios. The ultrasonication effect was characterized by visual appearance, zeta potential, particle size, FTIR, SEM, and EDS analyses. Additionally, the impact of particle size on the cytotoxicity and the encapsulation properties of a curcumin microemulsion was assessed. Chitosan ionization degree was pH dependent and was fully ionized below 4.0. The milliequivalent points were estimated at 0.9 and 1.06 for the two crosslinkers and chitosan, respectively. The maximum turbidity observed during complex formation was 0.5 and 1.0 for the pyrophosphate-chitosan and tripolyphosphate-chitosan samples, respectively, showing a critical conductivity value of 1. FTIR showed the interaction between phosphate salts (PO^–) and chitosan amino groups (NH₃⁺) in the region of 1200–1650 cm^–1. SEM showed that the particle size decreased with ultrasonication. After ultrasonication, the particle size of the phosphate-chitosan microparticles decreased 17-fold but the zeta potential did not change. The phosphate-chitosan complexes did not present cytotoxic activity at concentrations lower than 1000 µg/mL. The pyrophosphate-chitosan and tripolyphosphate-chitosan microparticles have remarkable loading capacities for curcumin, making them promising carriers for nutraceutical and pharmaceutical applications.</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"1 ","pages":"Article 100023"},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950069924000173/pdfft?md5=e72409404359cecd8192def8a8ab9342&pid=1-s2.0-S2950069924000173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of fermented soy sauce and blended soy sauce based on dielectric spectra","authors":"Yingman Xie,&nbsp;Jiayao Zhao,&nbsp;Chao Mao,&nbsp;Huiyun Pang,&nbsp;Pengfei Ye,&nbsp;Xiangwei Chen,&nbsp;Hongfei Fu,&nbsp;Yequn Wang,&nbsp;Yunyang Wang","doi":"10.1016/j.foodp.2024.100020","DOIUrl":"10.1016/j.foodp.2024.100020","url":null,"abstract":"<div><p>A new method based on dielectric properties has been developed to distinguish blended soy sauce from fermented soy sauce. Fifty samples of pure fermented soy sauce and blended soy sauce with different contents of hydrolyzed vegetable protein (HVP) were prepared. The dielectric constant and dielectric loss factor of all soy sauce samples in the 30 MHz–3000 MHz frequency range were measured using an impedance analyzer. The sample set was divided into a correction set and a prediction set using the joint x-y distances (SPXY) algorithm, and the partial least squares (PLS) and support vector machine (SVM) models were adopted to distinguish the different samples. The effects of selecting characteristic variables on model prediction using the full spectra (FS), principal component analysis (PCA), and successive projection algorithm (SPA) were compared. Results indicate that the discriminant effect of the PLS model was better than that of the SVM model overall. The SPA–PLS model had the best predictive performance among the six developmental models. The correlation coefficients of the correction set and prediction set were 0.9205 and 0.9096, respectively. The root mean square error of the calibration set was 1.3699 and that of the prediction set was 1.5950. The study demonstrated that the combination of dielectric spectra and stoichiometry can be utilized to determine whether soy sauce has been adulterated.</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"1 ","pages":"Article 100020"},"PeriodicalIF":0.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950069924000148/pdfft?md5=9580f4c96e13eb440b534bdc96a1b562&pid=1-s2.0-S2950069924000148-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141849497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transglutaminase mediated cross-linking of peanut and buckwheat proteins under dual-frequency ultrasound field: Functional attributes study","authors":"Xuan Zhang ,&nbsp;Yu Chen ,&nbsp;Yu Hu ,&nbsp;Jian Jin ,&nbsp;Hui lin Shen ,&nbsp;Chibuike C. Udenigwe","doi":"10.1016/j.foodp.2024.100019","DOIUrl":"10.1016/j.foodp.2024.100019","url":null,"abstract":"<div><p>Peanut protein (PP)/buckwheat protein (BWP) mixture crosslinked by transglutaminase (TGase) under dual-frequency ultrasound (DFU) field was developed and its structural and functional properties were characterized. The results showed that the optimal DFU parameters for PP-BWP crosslinking were 25 kHz/40 kHz dual-frequency ultrasound working time ratio of 10:10 (s/s), a temperature of 45°C, ultrasonic power density of 125 W/L and time duration of 30 min, resulting in the degree of crosslinking (DC) of 37.7 %, which is 125 % higher than that of the control. The solubility at pH 7.0, oil-holding capacity, and emulsifying capacity of the protein aggregate (peanut protein-buckwheat protein crosslinked by TGase under dual-frequency ultrasound, U-TG-PP-BWP) was significantly (<em>P</em>&lt;0.05) improved compared with protein aggregates formed without ultrasound (TG-PP-BWP); however, the emulsion stability, foaming capacity and form stability decreased. Secondary structure analysis indicated no apparent difference between un-crosslinked protein and U-TG-PP-BWP. After ultrasound treatment, the α-helix decreased by 11.2 % and the β-sheet, β-turn and random coil increased by 7.7 %, 3.4 % and 4.7 %, respectively, in U-TG-PP-BWP compared to TG-PP-BWP. The microstructures showed that U-TG-PP-BWP formed a lamellar structure with many micropores while TG-PP-BWP aggregated into large and tight blocks. SDS-PAGE analysis showed that, compared to PP and BWP, the subunit bands of cross-linked TG-PP-BWP and U-TG-PP-BWP were altered at 14.4–116 kDa, yielding proteins larger than 116 kDa, suggesting covalent cross-linking of the two proteins. In conclusion, the enzymatic cross-linking of proteins can be promoted by DFU, providing a new approach to developing novel plant proteins.</p></div>","PeriodicalId":100545,"journal":{"name":"Food Physics","volume":"1 ","pages":"Article 100019"},"PeriodicalIF":0.0,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950069924000136/pdfft?md5=081c15b748ec139d6e41097a2a6a19cb&pid=1-s2.0-S2950069924000136-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141622984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}