Fibrinolysis and Proteolysis最新文献

Endogeneous fibrinolysis and restenosis of peripheral arteries after percutaneous transluminal angioplasty 经皮腔内血管成形术后外周动脉内源性纤维蛋白溶解和再狭窄

Fibrinolysis and Proteolysis Pub Date : 2001-01-01 DOI: 10.1054/fipr.2001.0098

M. Kozak , P. Poredoš

引用次数: 0

Impaired liver regeneration after partial hepatectomy in plasminogen deficient mice 纤溶酶原缺陷小鼠部分肝切除术后肝脏再生受损

Fibrinolysis and Proteolysis Pub Date : 2001-01-01 DOI: 10.1054/fipr.2001.0097

M. Tanaka , K. Okada , S. Ueshima , M. Imano , H. Ohyanagi , P. Carmeliet , O. Matsuo

{"title":"Impaired liver regeneration after partial hepatectomy in plasminogen deficient mice","authors":"M. Tanaka , K. Okada , S. Ueshima , M. Imano , H. Ohyanagi , P. Carmeliet , O. Matsuo","doi":"10.1054/fipr.2001.0097","DOIUrl":"https://doi.org/10.1054/fipr.2001.0097","url":null,"abstract":"Abstract Liver regeneration is regulated by variety of growth factors. Release and activation of these growth factors are deeply related to degradation of extracellular matrix (ECM), which is regulated by plasminogen-activating system. Since plasminogen is assum ed to be essential in these functions in liver regeneration, partial 70% hepatectomy (PH) was performed on plasminogen deficient (Plg−/−) mice and wild-type (Plg+/+) mice. The sequential recovery of the liver weight after 70% PH gradually increased over 1 to 14 days in Plg+/+ mice. However, in Plg−/− mice, it increased over 1 to 7 days and thereafter increased no more. Thus, the recovery in Plg−/− mice was significantly impaired at 10 and 14 days compared with Plg+/+ mice. Plg+/+ mice exhibited an increase in DNA synthesis over5 days, and then a decrease thereafter; however, in Plg−/− mice, it increased over 3 days, and decreased thereafter, with the significant decrease at 14 days. The apoptotic nuclei labelling index slightly increased over 3 days, and decreased thereafter in Plg+/+ mice. In Plg−/− mice, it was few detected over 3 days, and gradually increased over 7 to 14 days. The apoptotic index in Plg−/− mice was higher than Plg+/+ mice at 14 days. In the histological examination of liver, focal area of cellular loss with fibrin deposition were detected in Plg−/− mice after PH, which were rarely detected in Plg+/+ mice. In Plg−/− mice, expressions of t-PA, u-PA and pro-MMP-9 were increased in the liver at 14 days after PH compared with Plg+/+ mice. These findings suggest that plasminogen plays an important role in liver regeneration after PH.","PeriodicalId":100526,"journal":{"name":"Fibrinolysis and Proteolysis","volume":"15 1","pages":"2-8"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/fipr.2001.0097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71806063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Covariance of metabolic and hemostatic risk indicators in men and women 男性和女性代谢和止血危险指标的协方差

Fibrinolysis and Proteolysis Pub Date : 2001-01-01 DOI: 10.1054/fipr.2001.0099

H. Riese , T.G.M. Vrijkotte , P. Meijer , C. Kluft , E.J.C. De Geus

{"title":"Covariance of metabolic and hemostatic risk indicators in men and women","authors":"H. Riese , T.G.M. Vrijkotte , P. Meijer , C. Kluft , E.J.C. De Geus","doi":"10.1054/fipr.2001.0099","DOIUrl":"https://doi.org/10.1054/fipr.2001.0099","url":null,"abstract":"Abstract Background and objective: Multivariate analyses on clusters of metabolic and hemostatic risk indicators implicitly assume good test–retest reliability of these variables, substantial covariance among the various indicators, stability of covariance structure over time, and comparable covariance structure in different subpopulations. The aim of the present study is to investigate these assumptions. Methods: Repeated samples were taken of fasting insulin, triglycerides (TG), high-density cholesterol (HDL-C), low-density cholesterol (LDL-C), fibrinogen, tissue-type plasminogen activator (t-PA) antigen, t-PA activity, plasminogen activator inhibitor-1 (PAI-1)antigen to address their intra-week reliability and covariance structure. In the same workweek blood was drawn three times from 125 sedentary males (age 45.2±5.3 years) and twice from 132 female nurses (age 33.7±8.0 years). Results: About half(44.8%) of these women were oral contraceptives (OC) users. Only minor intra-week changes in absolute levels were found. Intra-week test-retest correlations varied between 0.52 (t-PA activity) and 0.94 (HDL-C) with an average value of 0.81. In men, and non-OC using women, and OC using women, the covariance matrices of the eight risk indicators were equal at day 1 and day 3, testifying the good stability of covariance structure over time. Differences in covariance structure of all three groups were observed, which remained after correction for BMI and age. In men and non-OC-using women, significant correlation was found on all days between insulin and the other risk indicators with exception of fibrinogen and LDL-C. In OC users, insulin was correlated with TG, LDL-C, and fibrinogen. Conclusion: The metabolic and hemostatic risk indicators showed good test-retest reliability, and their covariance is stable over time. Multivariate analyses of this cluster should be performed separately for men, non-OC-using women, and OC-using women.","PeriodicalId":100526,"journal":{"name":"Fibrinolysis and Proteolysis","volume":"15 1","pages":"9-20"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/fipr.2001.0099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71847172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effects of low-molecular-weight heparin treatment on fibrinolytic markers in unstable coronary artery disease 低分子肝素治疗对不稳定冠状动脉疾病患者纤溶标志物的影响

Fibrinolysis and Proteolysis Pub Date : 2001-01-01 DOI: 10.1054/fipr.2001.0100

H. Toss , L. Wallentin , A. Siegbahn

{"title":"Effects of low-molecular-weight heparin treatment on fibrinolytic markers in unstable coronary artery disease","authors":"H. Toss , L. Wallentin , A. Siegbahn","doi":"10.1054/fipr.2001.0100","DOIUrl":"https://doi.org/10.1054/fipr.2001.0100","url":null,"abstract":"Abstract Objectives : Low-molecular-weight heparins (LMWHs) efficiently reduce the coagulant activity, but their influence on the fibrinolytic system and fibrin turnover is not fully elucidated. We therefore determined markers offibrinolytic activity, fibrin turnover and inflammation in LMWH treated patients with acute coronary artery disease. Design : Double-blind placebo controlled clinical trial. Setting : Consecutive patients admitted at two centers. Subjects : Individuals with unstable angina or non-Q-wave MI ( n = 87). Interventions : Randomized to placebo-controlled subcutaneous dalteparin treatment, 120 IU/kg bw twice daily for 5–8 days and 7500IU once daily over the next 35–45 days. Main outcome measures : Markers of fibrinolytic activity, i.e. tissue plasminogen activator (t-PA) antigen, plasminogen activator inhibitor 1 (PAI-1) activity, plasminogen anti-plasmin (PAP) complex, and D-dimer determined at inclusion, after 2, 5 and 40–50 days. C-reactive protein (CRP), fibrinogen and prothrombin fragment 1+2 (F1+2) were determined at inclusion. Results : At inclusion there were positive correlations between fibrinogen, CRP and D-dimer levels. During LMWH treatment there was a marked increase in t-PA antigen concentration, a rise in PAI-1 activity and a slight decrease in PAP-complex levels. The LMWH administration was also associated with a long-lasting decrease in the D-dimer concentrations. In the placebo group there was a slight and transient increase in all the evaluated markers of fibrinolytic activity and fibrin turnover. Conclusion : In unstable CAD increased inflammatory activity is associated with increased thrombin generation and fibrin turnover. During LMWH treatment there is a sustained reduction in fibrin turnover and no rise in plasmin generation despite an increase in t-PA level.","PeriodicalId":100526,"journal":{"name":"Fibrinolysis and Proteolysis","volume":"15 1","pages":"26-31"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/fipr.2001.0100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71847176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Differences in invasion between human smooth muscle cells from umbilical vein, saphenous vein and internal mammary artery: relation to the expression of the plasminogen activation system 人脐静脉、隐静脉和乳腺内动脉平滑肌细胞侵袭的差异:与纤溶酶原激活系统的表达有关

Fibrinolysis and Proteolysis Pub Date : 2000-11-01 DOI: 10.1054/fipr.2000.0092

M.J. Wijnberg, L.G.M. Huisman, J.H. Verheijen

{"title":"Differences in invasion between human smooth muscle cells from umbilical vein, saphenous vein and internal mammary artery: relation to the expression of the plasminogen activation system","authors":"M.J. Wijnberg, L.G.M. Huisman, J.H. Verheijen","doi":"10.1054/fipr.2000.0092","DOIUrl":"https://doi.org/10.1054/fipr.2000.0092","url":null,"abstract":"<div><p>Smooth muscle cells can express a range of phenotypes. Smooth muscle cells from intimal thickenings are phenotypically different from media smooth muscle cells. Migration and proliferation are important processes involved in the development of intimal t hickening. Several studies have demonstrated the role of the plasminogen activation system in the migration and proliferation of smooth muscle cells. In this study we determined the proliferation and invasion capacity of smooth muscle cells (SMC) that we re isolated from three different types of vessels; saphenous vein (SV), internal mammary artery (IMA), and umbilical vein (UV). Whereas we found no differences in proliferation, we show that there are clear differences in the expression of t-PA, u-PA, an d PAI-1 and subsequent differences in plasminogen activator activity between the smooth muscle cell types. Whereas u-PA activity was low in all three cell types studied, t-PA activity was lowest in UV-SMC (0.25IU/10<sup>6</sup>cells), and higher in SV-SMC (0.8IU/10<sup>6</sup>cells) and IMA-SMC (1.2IU/10<sup>6</sup>cells). These findings could in part explain the differences we found in the invasion capacity of the smooth muscle cell types in an in vitro matrix invasion assay. Whereas the invasion of SV-SMC could be inhibited by an inhibitor of plasmin (aprotinin), the invasion of UV-SMC could not. Furthermore, the invasion of UV-SMC could be stimulated by active t-PA or u-PA, whereas the invasion of SV-SMC could not. The results for IMA-SMC were, however, more variable. These findings toget her suggest that phenotypically different smooth muscle cells exist between different vessel types, and may explain some of the differences in the ability of vessels to develop intimal thickening.</p></div>","PeriodicalId":100526,"journal":{"name":"Fibrinolysis and Proteolysis","volume":"14 6","pages":"Pages 358-365"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/fipr.2000.0092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71829150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Differences in invasion between human smooth muscle cells from umbilical vein, saphenous vein and internal mammary artery: relation to the expression of the plasminogen activation system 人脐静脉、隐静脉和乳腺内动脉平滑肌细胞侵袭的差异:与纤溶酶原激活系统的表达有关

Fibrinolysis and Proteolysis Pub Date : 2000-11-01 DOI: 10.1054/FIPR.2000.0092

M. Wijnberg, L. Huisman, J. Verheijen

{"title":"Differences in invasion between human smooth muscle cells from umbilical vein, saphenous vein and internal mammary artery: relation to the expression of the plasminogen activation system","authors":"M. Wijnberg, L. Huisman, J. Verheijen","doi":"10.1054/FIPR.2000.0092","DOIUrl":"https://doi.org/10.1054/FIPR.2000.0092","url":null,"abstract":"Smooth muscle cells can express a range of phenotypes. Smooth muscle cells from intimal thickenings are phenotypically different from media smooth muscle cells. Migration and proliferation are important processes involved in the development of intimal t hickening. Several studies have demonstrated the role of the plasminogen activation system in the migration and proliferation of smooth muscle cells. In this study we determined the proliferation and invasion capacity of smooth muscle cells (SMC) that we re isolated from three different types of vessels; saphenous vein (SV), internal mammary artery (IMA), and umbilical vein (UV). Whereas we found no differences in proliferation, we show that there are clear differences in the expression of t-PA, u-PA, an d PAI-1 and subsequent differences in plasminogen activator activity between the smooth muscle cell types. Whereas u-PA activity was low in all three cell types studied, t-PA activity was lowest in UV-SMC (0.25IU/106cells), and higher in SV-SMC (0.8IU/106cells) and IMA-SMC (1.2IU/106cells). These findings could in part explain the differences we found in the invasion capacity of the smooth muscle cell types in an in vitro matrix invasion assay. Whereas the invasion of SV-SMC could be inhibited by an inhibitor of plasmin (aprotinin), the invasion of UV-SMC could not. Furthermore, the invasion of UV-SMC could be stimulated by active t-PA or u-PA, whereas the invasion of SV-SMC could not. The results for IMA-SMC were, however, more variable. These findings toget her suggest that phenotypically different smooth muscle cells exist between different vessel types, and may explain some of the differences in the ability of vessels to develop intimal thickening.","PeriodicalId":100526,"journal":{"name":"Fibrinolysis and Proteolysis","volume":"93 1","pages":"358-365"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86909627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

International Society for Fibrinolysis and Proteolysis 国际纤维蛋白溶解和蛋白溶解学会

Fibrinolysis and Proteolysis Pub Date : 2000-11-01 DOI: 10.1054/fipr.2000.0096

引用次数: 0

Rapid detection of streptokinase resistance using a bedside lytic assay of dry reagent technology 干试剂床边裂解法快速检测链激酶耐药

Fibrinolysis and Proteolysis Pub Date : 2000-11-01 DOI: 10.1054/FIPR.2000.0094

K. A. Shwafi, J. Renkin, A. Meester, B. Pirenne, J. Col

{"title":"Rapid detection of streptokinase resistance using a bedside lytic assay of dry reagent technology","authors":"K. A. Shwafi, J. Renkin, A. Meester, B. Pirenne, J. Col","doi":"10.1054/FIPR.2000.0094","DOIUrl":"https://doi.org/10.1054/FIPR.2000.0094","url":null,"abstract":"A new bedside lytic assay using dry reagent technology for rapid (3-5 min) detection of streptokinase resistance (SKR) was recently introduced, which measures lysis onset time (LOT) of whole blood clot in response to high and low streptokinase (SK) concentrations: 100 U/ml (SK100) and 10 U/ml (SK10). SKR was defined by prolongation of LOT, previously correlated with the standard SK Reactivity Test and with clinical outcome of acute myocardial infarction (AMI) SK-treated patients, high SKR when SK100>50 seconds and SK10>120 seconds; partial SKR when SK10>120 seconds. Five prospective clinical groups (325 patients) were screened in cardiac units of four university hospitals, In patients previously treated with SK, the prevalence of SKR was 87% (70% high, 17% partial); in those who had documented streptococcal infection, 92% (75% high, 17% partial); and in patients with rheumatic heart disease, 76% (all high). SKR prevalence was 55% (33% high, 22% partial) in those with recent respiratory tract infection. In 225 acute coronary patients, SKR was 28% (21% high, 7% partial), and was identical by gender, but was 36% (32% high, 4% partial) in patients greater than or equal to 65 years Versus 19% (9% high, 10% partial) in those < 65 years (P < 0.0001). In conclusion, we demonstrated (with a rapid functional assay) the consistence of our results with the expected prevalence of SKR in the groups studied, this points out to the feasibility of pre-therapeutic detection of SKR and choice between t-PA and SK made at bedside without delaying the onset of treatment. As SKR is common among candidates for thrombolysis, pre-therapeutic detection of SKR merits further investigation. (C) 2000 Harcourt Publishers Ltd.","PeriodicalId":100526,"journal":{"name":"Fibrinolysis and Proteolysis","volume":"14 1","pages":"351-357"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81825875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring of haemostatic parameters during thrombolysis with rtPA for deep venous thrombosis: correlation with clinical events rtPA溶栓治疗深静脉血栓的止血参数监测:与临床事件的相关性

Fibrinolysis and Proteolysis Pub Date : 2000-11-01 DOI: 10.1054/fipr.2000.0093

M. Grünewald , M. Griesshammer , D. Ellbrück , E. Seifried

{"title":"Monitoring of haemostatic parameters during thrombolysis with rtPA for deep venous thrombosis: correlation with clinical events","authors":"M. Grünewald , M. Griesshammer , D. Ellbrück , E. Seifried","doi":"10.1054/fipr.2000.0093","DOIUrl":"https://doi.org/10.1054/fipr.2000.0093","url":null,"abstract":"<div><p>In a substudy on patients undergoing thrombolytic therapy for deep venous thrombosis with different doses of recombinant tissue-type plasminogen activator (Alteplase; Actilyse®, Boehringer Ingelheim, Germany) within a multi-centre trial, several haemostatic parameters were determined serially in an attempt to correlate changes of these parameters with clinical events, such as therapeutic outcome and bleeding complications.</p><p>The main finding of our study was that the consumption of the inhibitors of fibrinolytic activity, PAI-1 and plasmin-inhibitor (formerly α2-antiplasmin) during continuous thrombolysis for deep venous thrombosis was associated with a significant increase of bleeding complications. In addition we found a trend towards lower recanalization rates and more frequent bleeding complications in patients with enhanced activation of the plasmatic coagulation system, reflected by higher concentrations of the activation peptides thrombin-antithrombin-complex, fibrin(ogen)-degradation-product and d-dimer.</p><p>As bleeding represents the major limitation to a wider application of thrombolytic therapy in deep vein thrombosis it might be worthwhile to evaluate a concept of individualized thrombolytic therapy, adjusted for parameters associated with enhanced bleeding risk and low recanalization rates.</p></div>","PeriodicalId":100526,"journal":{"name":"Fibrinolysis and Proteolysis","volume":"14 6","pages":"Pages 343-350"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/fipr.2000.0093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71829428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Monitoring of haemostatic parameters during thrombolysis with rtPA for deep venous thrombosis: correlation with clinical events rtPA溶栓治疗深静脉血栓的止血参数监测:与临床事件的相关性

Fibrinolysis and Proteolysis Pub Date : 2000-11-01 DOI: 10.1054/FIPR.2000.0093

M. Grünewald, M. Griesshammer, D. Ellbrück, E. Seifried

{"title":"Monitoring of haemostatic parameters during thrombolysis with rtPA for deep venous thrombosis: correlation with clinical events","authors":"M. Grünewald, M. Griesshammer, D. Ellbrück, E. Seifried","doi":"10.1054/FIPR.2000.0093","DOIUrl":"https://doi.org/10.1054/FIPR.2000.0093","url":null,"abstract":"Abstract In a substudy on patients undergoing thrombolytic therapy for deep venous thrombosis with different doses of recombinant tissue-type plasminogen activator (Alteplase; Actilyse®, Boehringer Ingelheim, Germany) within a multi-centre trial, several haemostatic parameters were determined serially in an attempt to correlate changes of these parameters with clinical events, such as therapeutic outcome and bleeding complications. The main finding of our study was that the consumption of the inhibitors of fibrinolytic activity, PAI-1 and plasmin-inhibitor (formerly α2-antiplasmin) during continuous thrombolysis for deep venous thrombosis was associated with a significant increase of bleeding complications. In addition we found a trend towards lower recanalization rates and more frequent bleeding complications in patients with enhanced activation of the plasmatic coagulation system, reflected by higher concentrations of the activation peptides thrombin-antithrombin-complex, fibrin(ogen)-degradation-product and d-dimer. As bleeding represents the major limitation to a wider application of thrombolytic therapy in deep vein thrombosis it might be worthwhile to evaluate a concept of individualized thrombolytic therapy, adjusted for parameters associated with enhanced bleeding risk and low recanalization rates.","PeriodicalId":100526,"journal":{"name":"Fibrinolysis and Proteolysis","volume":"97 1","pages":"343-350"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85771362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1