Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry最新文献_第4页

A novel mechanism of antibiotic resistance: study of the complex state of peptides with bacterial Staphylococcus aureus ribosomes 一种新的抗生素耐药机制:多肽与金黄色葡萄球菌核糖体复合物状态的研究

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01316-0

Laurent Verdier , Josyane Gharbi-Benarous , Gildas Bertho , Pascale Mauvais , Jean-Pierre Girault

引用次数: 1

Conformational study of the complementary peptide to a B-cell epitope of the La/SSB autoantigen La/SSB自身抗原b细胞表位互补肽的构象研究

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01296-8

Christine Hemmerlin , Angélique Phan Chan Du , Zouhair Elhilali , Avrilia Moulia , Vassilios Tsikaris , Maria Sakarellos-Daitsiotis , Constantinos Sakarellos , Eleni Dotsika , Athanasios G Tzioufas , Haralampos M Moutsopoulos , Manh-Thong Cung

{"title":"Conformational study of the complementary peptide to a B-cell epitope of the La/SSB autoantigen","authors":"Christine Hemmerlin , Angélique Phan Chan Du , Zouhair Elhilali , Avrilia Moulia , Vassilios Tsikaris , Maria Sakarellos-Daitsiotis , Constantinos Sakarellos , Eleni Dotsika , Athanasios G Tzioufas , Haralampos M Moutsopoulos , Manh-Thong Cung","doi":"10.1016/S1387-1609(01)01296-8","DOIUrl":"10.1016/S1387-1609(01)01296-8","url":null,"abstract":"<div>Starting from the 20-mer peptide 289–308, one of the experimentally characterized B-cell epitopes of the La/SSB autoantigen, the complementary peptide cpl(289–308), encoded by the complementary RNA was designed. The conformational properties of the cpl(289–308) were investigated in DMSO solution with the combined use of NMR data (vicinal coupling constants, NOE effects and temperature coefficient values), molecular modelling calculations of energy minimization and molecular dynamics. MD calculations led to a folded structure in which a βI-turn, stabilized by the H8 amide proton to the F5 carbonyl hydrogen bond, was found for the F5P6S7H8 sequence, whereas two γ-turns, centred around the E15 and I18 residues respectively, were found in the C-terminal part of the peptide. In the whole crown folded structure of the peptide, the Y4, F5, H8, F9 and F10 aromatic side chains are situated on one side with the E13, E15, T17 and C20 side chains on the other. This 3D structure resembles and could mimic the binding site of an antibody.</div>","PeriodicalId":100305,"journal":{"name":"Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry","volume":"4 10","pages":"Pages 729-733"},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-1609(01)01296-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75697691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conformational analysis by NMR and molecular modelling of the 41–62 hydrophilic region of HIV-1 encoded virus protein U (Vpu). Effect of the phosphorylation on sites 52 and 56 HIV-1编码病毒蛋白U (Vpu) 41-62亲水性区的核磁共振构象分析和分子模型。52和56位点磷酸化的影响

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01320-2

Gaël Coadou , Nathalie Évrard-Todeschi , Josyane Gharbi-Benarous , Richard Benarous , Jean-Pierre Girault

{"title":"Conformational analysis by NMR and molecular modelling of the 41–62 hydrophilic region of HIV-1 encoded virus protein U (Vpu). Effect of the phosphorylation on sites 52 and 56","authors":"Gaël Coadou , Nathalie Évrard-Todeschi , Josyane Gharbi-Benarous , Richard Benarous , Jean-Pierre Girault","doi":"10.1016/S1387-1609(01)01320-2","DOIUrl":"10.1016/S1387-1609(01)01320-2","url":null,"abstract":"<div>The peptide of 22 amino acid residues, Vpu_P41–62, phosphorylated at the two sites Ser52 and Ser56 has been implicated in the degradation of CD4 receptor molecules, an important stage of the pathways to human immunodeficiency virus type 1 pathology (HIV-1). In order to assess the structural influence of phosphorylation, a conformational analysis by NMR and molecular simulation have been carried out for the phosphorylated Vpu_P41–62, and non-phosphorylated Vpu41–62 in both H2O (at pH 3.5 and 7.2) and a 1:1 mixture of H2O and trifluoroethanol. Analysis of the short-, medium-range NOE connectivities and of the secondary chemical shifts indicated that the peptide segment (42–49) shows a less well-defined helix propensity. The 50–62 segment forms a loop with the phosphate group pointing away, a short β-strand and a flexible extended ‘tail’ of residues 60–62. Differences in this molecular region 50-62 suggest that conformational changes of Vpu_P, play a potential role in Vpu_P-induced degradation of CD4 molecules.</div>","PeriodicalId":100305,"journal":{"name":"Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry","volume":"4 10","pages":"Pages 751-758"},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-1609(01)01320-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73718321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Sequence determination and resonance assignments of an Azomonas siderophore using 13C natural abundance 13C–1H HNCA experiment 利用13C自然丰度13C - 1h HNCA实验测定氮单胞菌铁载体的序列和共振配位

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01294-4

Emeric Wasielewski , Mohamed A. Abdallah , Pavel Kyslik , Bruno Kieffer

引用次数: 1

IRM et SRM 1H-1D in vivo à 7 T d’un modèle de régénération musculaire sur la souris 小鼠肌肉再生模型体内7t 1H-1D mri和SRM

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01324-X

Catherine Sébrié, Brigitte Gillet, Jean-Claude Belœil

引用次数: 0

Recent developments in the characterization of water interacting with proteins by 17O NMR 17O核磁共振表征水与蛋白质相互作用的最新进展

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01293-2

Sandrine Besnard, Évelyne Baguet

引用次数: 1

Expression and purification of threonyl tRNA synthetase RNA binding domain for heteronuclear NMR studies 苏氨酸基tRNA合成酶RNA结合域的表达与纯化

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01295-6

Sophie Raibaud, Noémi Fukuhara , Frédéric Dardel

引用次数: 0

Use of heteronuclear multiple bond coherence NMR spectroscopy to monitor nitrogen metabolism in a transformed root culture of Datura stramonium 利用异核多键相干核磁共振光谱监测曼陀罗转化根培养中的氮代谢

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01318-4

Ophélie Fliniaux , François Mesnard , Sophie Raynaud , Sylvie Baltora , Richard J Robins , Marc-André Fliniaux

{"title":"Use of heteronuclear multiple bond coherence NMR spectroscopy to monitor nitrogen metabolism in a transformed root culture of Datura stramonium","authors":"Ophélie Fliniaux , François Mesnard , Sophie Raynaud , Sylvie Baltora , Richard J Robins , Marc-André Fliniaux","doi":"10.1016/S1387-1609(01)01318-4","DOIUrl":"10.1016/S1387-1609(01)01318-4","url":null,"abstract":"<div>In order to examine the factors that regulate the flux of primary metabolites into tropane alkaloids, Nuclear Magnetic Resonance spectroscopy (NMR) has been used to monitor nitrogen metabolism in transformed root cultures of Datura stramonium fed with (15NH4)2SO4 and K15NO3. This study employs the technique of Heteronuclear Multiple Bond Coherence (HMBC) NMR spectroscopy, which combines the advantages of both bidimensional resolution and 1H NMR sensitivity. Moreover, the HMBC sequence allows the 15N bound to labile protons to be observed via 2J and/or 3J couplings, which is very appropriate for the observation of tropane alkaloids. In transformed roots of Datura stramonium, in addition to the amino acids normally observed in monodimensional 15N NMR, other amino acids are resolved by HMBC. Labelled peaks due to N-acetyl compounds and to uridine are identified. While these primary metabolites were also seen in cell cultures of Nicotiana plumbaginifolia, cross peaks corresponding to secondary metabolites, such as tropine, were also observed. This is the first time that such secondary products have been found by this technique.</div>","PeriodicalId":100305,"journal":{"name":"Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry","volume":"4 10","pages":"Pages 775-778"},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-1609(01)01318-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84327973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Rôle des ponts disulfure dans le repliement en épingle à cheveux de l’androctonine. Relations structure–activité 二硫桥在雄激素发夹折叠中的作用。结构—活动的关系

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01297-X

Nicolas Mandard , Henri Labbé , Pedro Da Silva , Charles Hetru , Céline Landon , Françoise Vovelle

引用次数: 0

Avant-propos

Comptes Rendus de l'Académie des Sciences - Series IIC - Chemistry Pub Date : 2001-10-01 DOI: 10.1016/S1387-1609(01)01335-4

Arlette Trokiner présidente du GERM, Arlette Trokiner president of GERM

引用次数: 0