Comparative Biochemistry and Physiology Part B: Comparative Biochemistry最新文献_第2页

Antibacterial protection in Marthasterias glacialis eggs: characterization of lysozyme-like activity 冰马氏菌卵的抗菌保护作用:溶菌酶样活性的表征

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90134-1

L. Stabili, P. Pagliara

引用次数: 26

Glutamine and glucose metabolism in intraepithelial lymphocytes from pre- and post-weaning pigs 断奶前后仔猪上皮内淋巴细胞的谷氨酰胺和葡萄糖代谢

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90130-9

M.E.R. Dugan, D.A. Knabe, G. Wu

{"title":"Glutamine and glucose metabolism in intraepithelial lymphocytes from pre- and post-weaning pigs","authors":"M.E.R. Dugan, D.A. Knabe, G. Wu","doi":"10.1016/0305-0491(94)90130-9","DOIUrl":"10.1016/0305-0491(94)90130-9","url":null,"abstract":"<div>The metabolism of glutamine (Gln) and glucose was studied in intraepithelial lymphocytes (IEL) from 21-, 29- and 56-day-old pigs. Pigs were weaned at 21 days of age. Cells were incubated at 37°C in the presence of Krebs-Ringer bicarbonate buffer (pH 7.4) containing 1 mM [U14C]glutamine plus 5 mM glucose, or 5 mM [U14C]glucose plus 1 mM glutamine. Glucose was converted to lactate, pyruvate and CO2, which accounted for 81, 11 and 8% of measured glucose carbon, respectively. Glutamine was metabolized mainly to glutamate (92% of Gln C) and ammonia, and to a lesser extent, to aspartate (4% of Gln C) and CO2 (4% of Gln C). In the presence of both glucose and glutamine, glucose provided 2–3-fold more ATP to IELs than glutamine in 21–56-day-old pigs, on the basis of their measured end products. The rates of ammonia and glutamate production from glutamine in IELs from 29-day-old pigs were 112 and 90% greater than those in cells from 56-day-old pigs, respectively. The rates of glucose oxidation to CO2 in IELs from 29-day-old pigs were elevated 56 and 64% respectively, compared with 21- and 56-day-old pigs. Elevated rates of substrate metabolism in IELs from 29-day-old post-weaning pigs indicated a metabolic alteration of these cells possibly due to changes in diet and intestinal bacterial population.</div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 4","pages":"Pages 675-681"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90130-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18879980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Purification and establishment of ELISA for vitellogenin of Japanese sardine (Sardinops melanostictus) 日本沙丁鱼(Sardinops melanotictus)卵黄蛋白原酶联免疫吸附试验的纯化与建立

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90116-3

Takahiro Matsubara , Tokio Wada , Akihiko Hara

引用次数: 27

NAD-dependent glutamate dehydrogenase from the thermophilic eubacterium Bacillus acidocaldarius 嗜热嗜酸芽孢杆菌的nadd依赖性谷氨酸脱氢酶

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90132-5

Valerio Consalvi , Roberta Chiaraluce , Stefania Millevoi , Alessandra Pasquo , Laura Politi , Mario De Rosa , Roberto Scandurra

引用次数: 3

Volume contents, subject and author index volume 109 partB, 1994 卷的内容、主题和作者索引，第109卷b部分，1994年

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90139-2

引用次数: 0

Purification, characterization and amino terminal sequence of the superoxide dismutase from Babesia hylomysci 巴贝斯虫超氧化物歧化酶的纯化、鉴定及氨基末端序列分析

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90126-0

P. Becuwe , J. van Beeumen , B. Samyn , J.P. Touzel , C. Slomianny , D. Camus , D. Dive

引用次数: 5

Demonstration and preliminary characterization of α-amylase in the sweetpotato whitefly, Bemisia tabaci (Aleyrodidae: Homoptera) 甘薯粉虱α-淀粉酶的鉴定与初步鉴定(粉虱科:同翅目)

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90122-8

Allen Carson Cohen, Donald L. Hendrix

{"title":"Demonstration and preliminary characterization of α-amylase in the sweetpotato whitefly, Bemisia tabaci (Aleyrodidae: Homoptera)","authors":"Allen Carson Cohen, Donald L. Hendrix","doi":"10.1016/0305-0491(94)90122-8","DOIUrl":"10.1016/0305-0491(94)90122-8","url":null,"abstract":"<div>An α-amylase that hydrolyzes unmodified starch or amylopectin azure was demonstrated in crude and partially purified extracts prepared from whole carcasses of sweetpotato whiteflies (SPW) (Bemisia tabaci Genn.).All nymphal instars and adult SPW, including newly eclosed crawlers that had not yet fed on plant materials, were found to have active α-amylase. α-Amylase activity per mg protein was greatest in 1st instars and decreased with age up to the “pupal” stage, with a very slight increase in activity in adults. However, activity per individual did not differ substantially as a function of age.The α-amylase had an apparent molecular weight of about 70 kDa, an isoelectric point of 6.32 and eluted with about 250 mM NaCl from a strongly basic anion-exchange column.The enzyme activity was inhibited by EDTA and not activated by either NaCl or KNO3. CaCl2 strongly enhanced activity.α-Amylase activity was greatest at pH 7.0, but there was considerable activity at pHs above 7.0.The Km of the α-amylase was 1.47 Mm with p-nitrophenyl α-d-malto-heptaoside as substrate.The presence of an amylolytic enzyme in a phloem-feeding insect is unexpected and raises questions about current assumptions of feeding behavior of this species.</div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 4","pages":"Pages 593-601"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90122-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91296388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Glutathione S-transferases from the gastrointestinal nematode Heligmosomoides polygyrus and mammalian liver compared 胃肠道线虫和哺乳动物肝脏中谷胱甘肽s转移酶的比较

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90121-X

Peter M. Brophy , Anne Ben-Smith , Alan Brown , Jerzy M. Behnke , David I. Pritchard

引用次数: 21

Relationship between the induction of proteins in the HSP70 family and thermosensitivity in two species of Oryzias (Pisces) 两种稻属植物HSP70家族蛋白诱导与热敏性的关系

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90127-9

Ayami Arai, Hiroshi Mitani, Kiyoshi Naruse, Akihiro Shima

引用次数: 23

In vitro biosynthesis of steroids in ovary of asynchronic Trichogaster trichopterus (Pallus, 1770) 非慢性trichoaster trichopterus卵巢内类固醇的体外生物合成(Pallus, 1770)

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI: 10.1016/0305-0491(94)90135-X

Gad Degani , Erez Gal , Jacob Vaya

引用次数: 10