Michael L. Montpetit , Timothy Gleeson , Francis F. Mandy
{"title":"Flow molecular cytobiology: The next generation?","authors":"Michael L. Montpetit , Timothy Gleeson , Francis F. Mandy","doi":"10.1016/S0197-1859(00)89062-7","DOIUrl":"10.1016/S0197-1859(00)89062-7","url":null,"abstract":"","PeriodicalId":100270,"journal":{"name":"Clinical Immunology Newsletter","volume":"18 11","pages":"Pages 135-139"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0197-1859(00)89062-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72572706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"New advances for serologic evaluation of celiac disease and dermatitis herpetiformis","authors":"PhD Walter L. Binder (Director of Research)","doi":"10.1016/S0197-1859(00)89061-5","DOIUrl":"10.1016/S0197-1859(00)89061-5","url":null,"abstract":"<div><p>The tTG ELISA shows comparable performance to the more traditional IgA endomysial antibody test using immunofluoresence and primate esophagus sections. The tTG ELISA has the advantage of being objective and is not adversely influenced by the presence of interfering antibodies such as antinuclear (ANA) and smooth muscle antibodies (ASMA). The test can be used to economically screen large numbers of symptomatic as well as asymptomatic but at risk individuals. The ELISA methodology will afford an opportunity to better standardize celiac serologies from laboratory to laboratory since the performance of the current endomysial test is highly dependent on the microscope used, the initial screening dilution, the fluorescein to protein (f/p) ratio of the anti IgA conjugate, and the microscopist interpreting the test. Another potential problem with the current immunofluorescent based endomysial test is the increasing scarcity of fresh, healthy primate tissue for producing the substrate slides. We envision the tTG ELISA as being an integral part of a serological work up for suspected celiac and dermatitis herpetiformis patients. The inclusion of tTG ELISA along with tests for gliadin IgA and IgG antibodies can provide a sensitive, specific and objective assessment of individuals to be considered for confirmatory biopsy or even a trial with gluten-free diet.</p></div>","PeriodicalId":100270,"journal":{"name":"Clinical Immunology Newsletter","volume":"18 11","pages":"Pages 125-134"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0197-1859(00)89061-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81011129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MD Lauren M. Pachman (Professor of Pediatrics, Chief)
{"title":"Clinical description and epidemiology data","authors":"MD Lauren M. Pachman (Professor of Pediatrics, Chief)","doi":"10.1016/S0197-1859(00)89058-5","DOIUrl":"10.1016/S0197-1859(00)89058-5","url":null,"abstract":"<div><p>Assessment of both the epidemiology of JDM and of the data obtained from sensitive diagnostic laboratory tools will provide useful and specific information about the pathophysiology of this most interesting disease. Clues to disease activity — the MRI, neopterin, vWF:Ag levels, and percentage of B cells — are still imperfect guides to therapy,<sup>44</sup> but may be of substantial aid in the characterization of the severity of the immunologically mediated inflammatory process.<sup>74</sup> A more comprehensive understanding of the genetic factors that are associated with either the disease onset or the course of JDM may help in forecasting the outcome of this illness.</p></div>","PeriodicalId":100270,"journal":{"name":"Clinical Immunology Newsletter","volume":"18 10","pages":"Pages 105, 112-118"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0197-1859(00)89058-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87193996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cytokines in children with arthritis","authors":"Esther Crawley, Patricia Woo","doi":"10.1016/S0197-1859(00)89057-3","DOIUrl":"10.1016/S0197-1859(00)89057-3","url":null,"abstract":"<div><p>An imbalance between pro- and anti-inflammatory cytokines appears to exist in children with JIA. More abundant anti-inflammatory cytokines in children with persistent oligoarticular JIA partly may explain the relatively benign nature of this subgroup. Differences in the genetic control of transcription of IL-6 and IL-10 genes have been found to be associated with disease subgroups and may well be important in the pathogenesis or disease severity of these JIA subgroups. Further studies of other cytokine genes within the cytokine network are needed. Drugs used in the treatment of JIA may act partly by altering cytokine production and restoring cytokine homeostasis. In the future, genetic studies may allow one to give a patient a reliable prognosis based on accumulated odds ratios for different cytokine genotypes as well as other associated genes. The elucidation of cytokine imbalances will also provide molecular targets for the development of more specific and safer means of therapy.</p></div>","PeriodicalId":100270,"journal":{"name":"Clinical Immunology Newsletter","volume":"18 10","pages":"Pages 105-112"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0197-1859(00)89057-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81516217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The apo-1/fas death signaling pathway: a life and death balance","authors":"Steven K. Koester, Wade E. Bolton","doi":"10.1016/S0197-1859(00)89184-0","DOIUrl":"10.1016/S0197-1859(00)89184-0","url":null,"abstract":"","PeriodicalId":100270,"journal":{"name":"Clinical Immunology Newsletter","volume":"18 9","pages":"Pages 97-102"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0197-1859(00)89184-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77299633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phosphorylation of retinoblastoma protein (pRb) assayed in individual cells by multilaser flow cytometry","authors":"PhD Gloria Juan, MD Zbigniew Darzynkiewicz","doi":"10.1016/S0197-1859(00)89182-7","DOIUrl":"10.1016/S0197-1859(00)89182-7","url":null,"abstract":"","PeriodicalId":100270,"journal":{"name":"Clinical Immunology Newsletter","volume":"18 9","pages":"Pages 89-94"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0197-1859(00)89182-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87115292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Establishing reference ranges for immunophenotyping using three color flow cytometry","authors":"Thomas W, Mc Closkey, Savita Pahwa","doi":"10.1016/S0197-1859(00)89180-3","DOIUrl":"10.1016/S0197-1859(00)89180-3","url":null,"abstract":"","PeriodicalId":100270,"journal":{"name":"Clinical Immunology Newsletter","volume":"18 7","pages":"Pages 78-81"},"PeriodicalIF":0.0,"publicationDate":"1998-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0197-1859(00)89180-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85910940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}