Biomarkers and Genomic Medicine最新文献

{"title":"Up-regulation of the Mps one binder proteins-2 (mob2) gene enhances human fibrosarcoma cells migration by increasing matrix metalloproteinases expression","authors":"Kuan-Min Chen , Seng-sheen Fan , Wei-Ting Chao , Li-Yu Chen , Min-Huan Wu","doi":"10.1016/j.bgm.2014.09.013","DOIUrl":"10.1016/j.bgm.2014.09.013","url":null,"abstract":"","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 4","pages":"Page 199"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.09.013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75265142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperglycemia initiates N-cadherin rearrangement and diabetic monocytes promote inflammatory responses in human microvascular endothelial cells","authors":"Chi-Lun Feng,&nbsp;Hsiu-Chuan Chou","doi":"10.1016/j.bgm.2014.07.003","DOIUrl":"10.1016/j.bgm.2014.07.003","url":null,"abstract":"<div><p>The prevalence of diabetes around the world is increasing, and the complications of diabetes are becoming worse so that the global burden of diabetes-related complications is rising. The purpose of this study was to simulate the physiological condition of diabetes in the human body by culturing human microvascular endothelial cell 1 (HMEC-1) cells in Dulbecco’s modified Eagle’s medium (DMEM) containing 5.5mM glucose, 25mM glucose, and 50mM glucose. The cell viability of HMEC-1 cells at the indicated glucose concentrations was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The specific epithelial cell junction N-cadherin was measured by immunofluorescence. Furthermore, monocytic initiation of inflammatory reactions was studied by using Western blotting and enzyme-linked immunosorbent assay. Based on our results, hyperglycemia treatment influenced the distribution of N-cadherin-containing structures in HMEC-1 cells, whereas the change of the inflammatory profiles in HMEC-1 cells was affected after their coculture with the supernatant of diabetic THP-1 monocytes. In this study, we conclude that the removal of endothelial N-cadherin caused by hyperglycemia (at the 25mM glucose and 50mM glucose concentrations) may lead to endothelial dysfunction and subsequently endothelial cell death in the late stage of diabetes. Furthermore, the loss of intercellular adhesion molecule 1 and the upregulation of interleukin (IL)-1, IL-6, and tumor necrosis factor alpha mediated by diabetic monocytes showed altered interaction between the vascular endothelium and blood cells in the diabetic microenvironment.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 4","pages":"Pages 175-179"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.07.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75762938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Charnoly body as a universal biomarker of cell injury","authors":"Sushil Sharma ,&nbsp;Manuchair Ebadi","doi":"10.1016/j.bgm.2014.03.004","DOIUrl":"10.1016/j.bgm.2014.03.004","url":null,"abstract":"<div><p>The Charnoly body (CB) is a pleomorphic, electron-dense, multilamellar, preapoptotic, mitochondrial biomarker of cell injury. Nutritional stress and environmental toxins induce CB formation in highly vulnerable developing neurons because of compromised mitochondrial bioenergetics; however, nutritional rehabilitation, physiological zinc supplementation, and metallothioneins (MTs) inhibit CB formation. Accumulation of CBs at the junction of the axon hillock may impair the axoplasmic transport of ions, neurotransmitters, neurotropic factors, and enzymes at the synaptic terminals. Therefore, drugs may be developed to inhibit CB formation in neurodegenerative and cardiovascular diseases. In addition, nonspecific induction of CB formation in hyperproliferating cells with cancer chemotherapy causes as adverse effects alopecia, myelosuppression, gastrointestinal tract symptoms, cardiovascular toxicity, and infertility. Hence, drugs may be developed to induce cancer stem cell-specific CB formation to cure multidrug-resistant malignancies and chronic infections. Natural abundance and genetic susceptibility of mitochondrial DNA qualify CB as an early, unique, and sensitive universal biomarker of clinical significance.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 3","pages":"Pages 89-98"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.03.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79952136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-κB and PPAR-γ","authors":"Bambang Rahardjo ,&nbsp;Edy Widjajanto ,&nbsp;Hidayat Sujuti ,&nbsp;Kusnarman Keman","doi":"10.1016/j.bgm.2014.06.002","DOIUrl":"10.1016/j.bgm.2014.06.002","url":null,"abstract":"<div><p>The purpose of this study is to determine the role of curcumin in the alteration of levels of interleukin (IL)-1α, IL-6, and tumor necrosis factor α (TNF-α) as proinflammatory cytokines in monocyte culture exposed to preeclamptic plasma, as well as the effect on the transcription factors: nuclear factor kappa beta (NF-κB) and peroxisome proliferator-activated receptor γ (PPAR-γ). Plasma was taken from preeclamptic women (<em>n</em> = 12) and normotensive pregnant women (<em>n</em> = 12). Monocyte cultures were taken from nonpregnant healthy woman. Monocyte cultures were incubated with plasma for 48 hours. Curcumin in various doses were given in monocytic cultures prior to and after preeclamptic plasma exposure. The levels of IL-1α, IL-6, and TNF-α as well as NF-κB and PPAR-γ in each culture were determined by enzyme-linked immunosorbent assay procedures. The final data were analyzed by analysis of variance (ANOVA) and path analysis. This study shows a significant increase (<em>p</em> &lt; 0.05) in the levels of proinflammatory cytokines (IL-1α, IL-6, and TNFα) in monocyte cultures exposed to preeclamptic plasma compared with normotensive pregnancy plasma. Curcumin treatment in various doses can decrease significantly (<em>p</em> &lt; 0.05) proinflammatory cytokine levels in monocyte cultures that have been already stimulated by preeclamptic plasma. After curcumin treatment, there was a decreased level of nuclear NF-κB p50 and a significantly increased level of PPAR-γ. Curcumin has a direct effect on decreasing the levels of nuclear NF-κB p50 and also curcumin indirectly influenced the level of nuclear NF-κB p50 by the increased level of PPAR-γ. Curcumin could decrease levels of proinflammatory cytokines (IL-1α, IL-6, and TNFα) in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors, NF-κB and PPAR-γ. Curcumin has potential in the prevention and future treatment of preeclampsia, through inflammation pathways assumed as being responsible for the development of preeclampsia.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 3","pages":"Pages 105-115"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.06.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84540507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the LAMP-PCR-hybridization–thermal melt–ELISA method for molecular detection of multidrug resistance in Mycobacterium tuberculosis isolates","authors":"Mei-Feng Lee,&nbsp;Jing-Yu Chen,&nbsp;Chien-Fang Peng","doi":"10.1016/j.bgm.2014.05.002","DOIUrl":"10.1016/j.bgm.2014.05.002","url":null,"abstract":"<div><p>In this study, we designed a novel colorimetric method to detect multidrug resistance in <em>Mycobacterium tuberculosis</em> isolates. The assay of loop-mediated isothermal amplification (LAMP) is used to amplify target DNA from multidrug-resistant <em>M. tuberculosis</em> isolates, and enzyme-linked immunosorbent assay (ELISA) is used for the colorimetric determination. This method is designed based on point mutation at the hot spot region in target drug-resistant gene using LAMP-polymerase chain reaction (PCR), hybridization, and thermal melting for differentiating homoduplex DNA (drug-susceptible stain) and heteroduplex DNA (resistance mutant). From ELISA colorimetric detection, color change developed in drug-susceptible strains, and colorless result appeared in resistance mutants. A comparison of this LAMP-PCR-hybridization–thermal melt–ELISA (LAMP–TM–ELISA) method with the automated BACTEC MGIT 960 system showed that the sensitivity of this molecular analysis of resistance to isoniazid, rifampin, amikacin, and ciprofloxacin in <em>M. tuberculosis</em> was 92.3%, 95.3%, 93.1%, and 91.4%, respectively. This method for detection of resistance to isoniazid, rifampin, amikacin, and ciprofloxacin in <em>M. tuberculosis</em> showed a specificity of 95.5–98.2% and a test efficiency of 93.2–96.8%. This LAMP–TM–ELISA method will be a useful tool for rapid diagnosis (within 1 working day) and cost-effectiveness (US$15/reaction) to detect resistance to isoniazid, rifampin, amikacin, and ciprofloxacin via <em>katG</em>, <em>inhA</em> and <em>mabA</em>-<em>inhA</em> promoter, <em>rpoB</em>, <em>rrs</em>, <em>gyrA</em>, and <em>gyrB</em> genes in <em>M. tuberculosis</em> isolates.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 3","pages":"Pages 126-132"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85922341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Participation of hypoxia-inducible factor-1α in the pathogenesis of preeclampsia-related placental ischemia and its potential as a marker for preeclampsia","authors":"Meenakshi Akhilesh ,&nbsp;Vivekananda Mahalingam ,&nbsp;Sivalingam Nalliah ,&nbsp;Rosalina Mohd Ali ,&nbsp;Murali Ganesalingam ,&nbsp;Nagaraja Haleagrahara","doi":"10.1016/j.bgm.2014.04.002","DOIUrl":"10.1016/j.bgm.2014.04.002","url":null,"abstract":"<div><p>Hypoxia-inducible factor-1α (HIF-1α) is important for placental development. This study aims to determine whether the increased level and expression of HIF could be used to demonstrate failed placentation in women with preeclampsia. The study included 20 pregnant females [10 with and 10 without preeclampsia (the control group)]. Placental tissues were collected and stained with hematoxylin and eosin. Immunohistochemical studies for evaluating the expression of HIF-1α by these tissues were then performed. The results demonstrated that placental tissues collected from mothers with preeclampsia showed a variety of histomorphological changes. All the cytotrophoblasts rimming the placental villi in mothers with preeclampsia demonstrated a strong and uniform nuclear staining with HIF-1α. The study results indicated that cytotrophoblasts respond to an ischemic environment by their nuclear expression of HIF-1α, and thus we conclude that this transcription factor has a significant potential as a marker for preeclampsia.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 3","pages":"Pages 121-125"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.04.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75931765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyclonal antibody from 47 kDa protein of bladder cancer is sensitive and specific for detection of bladder cancer","authors":"Heru Prasetya ,&nbsp;Basuki Bambang Purnomo ,&nbsp;I. Ketut Gede Muliartha ,&nbsp;Sumarno Reto Prawiro","doi":"10.1016/j.bgm.2014.06.003","DOIUrl":"10.1016/j.bgm.2014.06.003","url":null,"abstract":"<div><p>This study aims to investigate whether the protein isolated from bladder cancer in an Indonesian population can produce the polyclonal antibody for clinical markers of bladder cancer. The participants in this study are bladder cancer patients and healthy persons who were approved for midstream portion urine collection. The inclusion criteria included bladder cancer patients who obtained hematuria examination, urine cytology, and initial therapy with transurethral resection of the bladder; a healthy volunteer who was approved for midstream portion urine collection but without hematuria history, bladder stones, and signs or symptoms of tractus urinarius infection. The procedure consisted of tissue preparation, tissue processing, isolation of membrane cell protein, monitoring of protein in membrane cell, production of polyclonal antibodies, and dot blot technique. A protein with 122 kDa molecular weight is present in epithelial cells of bladder cancer and the normal bladder. A protein with 69 kDa molecular weight is only present in the epithelial cell bladder of normal individuals. In addition, a protein with molecular weight of 47 kDa is only present in epithelial cells of bladder cancer. The minimal ratio of polyclonal antibody with antigen is 1:6400 of the antibody and 1:40 of antigen. Subsequently, this concentration was applied to detect proteins with 47 kDa only in several cancer tissues. Positive results in bladder cancer, but negative results in prostate cancer, rectal cancer, and breast cancer were found. The polyclonal antibody produced from 47 kDa protein subunit is sensitive and specific to detect bladder cancer and become an alternative biomarker for diagnosis and surveillance of bladder cancer.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 3","pages":"Pages 116-120"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.06.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91533966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subchronic inhalation of particulate matter 10 coal dust induces atherosclerosis in the aorta of diabetic and nondiabetic rats","authors":"Bambang Setiawan ,&nbsp;Nia Kania ,&nbsp;Dian Nugrahenny ,&nbsp;Nurdiana Nurdiana ,&nbsp;Moch. Aris Widodo","doi":"10.1016/j.bgm.2014.03.002","DOIUrl":"10.1016/j.bgm.2014.03.002","url":null,"abstract":"<div><p>This study aimed to elucidate whether subchronic inhalation of particulate matter (PM10) coal dust induces atherosclerosis in diabetic rats. A total of 32 male Wistar rats, were randomly divided into eight groups including four nondiabetic groups and four groups of diabetic rats. These rats were exposed to doses of coal dust equal to 0 mg/m³, 6.25 mg/m³, 12.5 mg/m³, or 25 mg/m³ for 1 hour/day for 28 days. Plasma levels of lipid peroxides were determined as thiobarbituric acid reactive substance. The levels of circulating endothelial cells were analyzed histologically. Foam cells formation was analyzed in aorta and tail artery with Oil Red O staining. Analysis of variance test was used to compare all parameters. Nondiabetic rats exposed to coal dust had significantly increased oxidative stress compared to the control group (<em>p</em> &lt; 0.05). Diabetic rats exposed to coal dust at dose of 25 mg/m³ had significantly increased oxidative stress compared to that of control diabetic rats (<em>p</em> &lt; 0.05). The levels of endothelial damage were significantly increased in diabetic rats exposed to coal dust at doses of 6.25 mg/m³ and 12.5 mg/m³ (<em>p</em> &lt; 0.05) compared to control diabetic rats. The foam cell counts were significantly increased in the aorta of nondiabetic rats exposed to coal dust at doses of 6.25 mg/m³ and 25 mg/m³ compared to control rats (<em>p</em> &lt; 0.05), also in the tail artery at dose of 25 mg/m³. The foam cell counts were significantly increased in the aorta of diabetic rats exposed to coal dust at doses of 6.25 mg/m³ and 12.5 mg/m³ than that in control diabetic rats (<em>p</em> &lt; 0.05). Subchronic inhalation of PM10 coal dust induces atherosclerosis through oxidative stress and endothelial damage in aorta of nondiabetic and diabetic rats.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 2","pages":"Pages 67-73"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.03.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87201471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The female-specific effect of 5-hydroxytryptamine receptor 3A gene on postoperative vomiting in Taiwan","authors":"Yi-Mei Joy Lin ,&nbsp;Cheng-Da Hsu ,&nbsp;Hsiao-Yen Hsieh ,&nbsp;Chia-Chih Alex Tseng","doi":"10.1016/j.bgm.2014.02.003","DOIUrl":"10.1016/j.bgm.2014.02.003","url":null,"abstract":"<div><p>Postoperative vomiting (POV) is a common complication after general anesthesia. Clarifying the genetic factors that affect POV are important for evaluating a patient's susceptibility to the condition. Although evidence suggests that the <em>5-hydroxytryptamine</em> (serotonin) <em>receptor 3A</em> (<em>HTR3A</em>) gene may be important in the occurrence of POV, associations for <em>HTR3A</em> polymorphisms with POV have not been investigated in a Taiwanese population. Three single nucleotide polymorphisms (SNPs) of the <em>HTR3A</em> gene were used to study the genetic association with POV in 369 postoperative Taiwanese adults who underwent general anesthesia. Although no significant differences were found at the single-locus level for <em>HTR3A</em> polymorphisms, a significant haplotype-based association was found between <em>HTR3A</em> and POV. In addition, because female sex is associated with a higher risk of PONV (postoperative nausea and vomiting), we separately analyzed the haplotypic associations for both sexes to test whether <em>HTR3A</em> genetic factors interact with female sex and specifically contribute to the etiology of POV. We found that a significant haplotype effect was identified only for females. The CTT haplotype, the most common, showed a significant protective effect (odds ratio: 0.68), and the CTG haplotype was associated with a significantly higher risk (odds ratio: 2.08) for POV in females. Furthermore, <em>p</em> values from an overall comparison of all haplotypes and from permutation tests were still significant. These data suggest that the <em>HTR3A</em> gene may have a sex-specific effect on the etiology of POV in Taiwan. The effects and the biological causal variants related to POV are worth additional investigation.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 2","pages":"Pages 59-66"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.02.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73663981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and characterization of class 1 integron-associated gene cassettes from Pseudomonas aeruginosa isolates in southern Taiwan","authors":"Ke-Yu Hsiao,&nbsp;Mei-Feng Lee,&nbsp;Chien-Fang Peng","doi":"10.1016/j.bgm.2014.02.004","DOIUrl":"10.1016/j.bgm.2014.02.004","url":null,"abstract":"<div><p>In this study, detection and characterization of class 1 integron-associated gene cassettes from <em>Pseudomonas aeruginosa</em> isolates in southern Taiwan were investigated. The study focused on the association between integron-associated resistance gene cassettes and multidrug resistance in <em>P. aeruginosa</em> isolates. Using polymerase chain reaction amplification, DNA sequencing, and basic local alignment search tool analysis, a total of 22 different types of gene cassette arrays were detected in 162 class 1 integron-positive <em>P. aeruginosa</em> isolates. We first identified 11 different types of new gene cassette arrays within class 1 integron in <em>P. aeruginosa</em> isolates, including <em>aac(6</em>′<em>)-II-catB2-aadA2</em>, <em>aac(6</em>′<em>)-II-aadA2</em>, <em>aac(6</em>′<em>)-II-catB2</em>, <em>aacA4-aadA15</em>, <em>aacC1-orfA-orfB-aadA1</em>, <em>cm1A-aadA1</em>, <em>catB3-bla</em>_OxA-10<em>-aadA15</em>, <em>aacA4-catB8-aadA1</em>, <em>aadB-orfF1-aadA11</em>, <em>dfrB1,</em> and <em>dfrB4a-aacA4-aacA4-aadA1</em>. Of these, <em>aac(6</em>′<em>)-II-catB2-aadA2</em> was the most frequently found gene cassette. Twenty-one (21/162, 12.9%) strains carrying two different types of gene cassette arrays of <em>catB3-bla</em>_OxA-10<em>-aadA15</em> and <em>aac(6</em>′<em>)-II-catB2-aadA2</em> were also simultaneously present in the <em>P. aeruginosa</em> isolates. A novel <em>dfrB4a</em> gene, different from the <em>dfrB4</em> gene detected in the gene cassette array of <em>dfrB4a-aacA4-aacA4-aadA</em>, was characterized. A metallo-β-lactamaseo gene was also found to be carried on the gene cassette of <em>bla</em>_VIM-3<em>-orf2a-aacA4-aadB-aacA4</em> inserted in a class 1 integron obtained from meropenem-resistant <em>P. aeruginosa</em> isolates.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"6 2","pages":"Pages 74-78"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2014.02.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80005621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}