Biomarkers and Genomic Medicine最新文献_第10页

Angiotensin II type I receptor modulates the migratory and invasive abilities of oral squamous cell carcinoma (OSCC) via MCP-1 signaling 血管紧张素II I型受体通过MCP-1信号调节口腔鳞状细胞癌(OSCC)的迁移和侵袭能力

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.bgm.2013.08.028

Chun-Chieh Yu , Chang-Han Chen

引用次数: 0

Ileus secondary to a retroperitoneal malignant melanoma 腹膜后恶性黑色素瘤继发肠梗阻

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.bgm.2013.08.001

Chin-Fan Chen , Chieh-Han Chuang , Ching Hu , Jaw-Yuan Wang

{"title":"Ileus secondary to a retroperitoneal malignant melanoma","authors":"Chin-Fan Chen , Chieh-Han Chuang , Ching Hu , Jaw-Yuan Wang","doi":"10.1016/j.bgm.2013.08.001","DOIUrl":"10.1016/j.bgm.2013.08.001","url":null,"abstract":"<div><p>Retroperitoneal malignant melanomas, either primary or metastatic, are rare. We present our clinical experience concerning one case with ileus secondary to a huge retroperitoneal malignant melanoma. A 77-year-old woman was admitted to the hospital due to progressive abdominal fullness and decreased appetite for 4 months. Plain films showed soft-tissue density in the left lower quadrant of the abdomen, as well as rightward displacement of the intestine. Laboratory data excluded any metabolic or septic causes of ileus. Abdominal computed tomography scan identified a huge retroperitoneal tumor with invasion of the left lower peritoneal space. The mass measured approximately 18.2 × 21.5 cm in the largest section. Immunohistochemical analysis of the tumor biopsies at minilaparotomy showed positive staining of tumor cells for S-100 protein, human melanoma black-45, and vimentin. Thus, a diagnosis of malignant melanoma with peritoneal metastases was established. This case highlights the possibility of a retroperitoneal malignant melanoma exerting a mass effect on the surrounding organs. The authors suggest that malignant melanoma should be taken into consideration as a possible differential diagnosis of retroperitoneal neoplasms.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"5 3","pages":"Pages 113-116"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2013.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82152459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hydroquinone-induced miR-122 down-regulation on the connection of ADAM17-mediated TNFα shedding 对苯二酚诱导的miR-122下调与adam17介导的TNFα脱落的关系

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.bgm.2013.08.007

Ying-Jung Chen, Long-Sen Chang

引用次数: 0

Role of asparagine synthetase in doxorubicin-induced resistance 天冬酰胺合成酶在阿霉素诱导耐药中的作用

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.bgm.2013.07.003

Li-Hsun Lin , Szu-Ting Lin , Hsiu-Chuan Chou

{"title":"Role of asparagine synthetase in doxorubicin-induced resistance","authors":"Li-Hsun Lin , Szu-Ting Lin , Hsiu-Chuan Chou","doi":"10.1016/j.bgm.2013.07.003","DOIUrl":"10.1016/j.bgm.2013.07.003","url":null,"abstract":"<div><p>Research has shown drug resistance as the major cause of failure of cancer chemotherapy. In this study, doxorubicin-sensitive human uterine cancer cell (hUCC) MES/SA, as well as doxorubicin-resistant hUCC MES/SA-DxR 2μM and MES/SA-DxR 8μM were used. Subsequently, asparagine synthetase (ASNS), a protein that had previously been proposed to be a putative cancer drug target in our laboratory, was silenced by siRNA knockdown to study the mechanism of doxorubicin-induced resistance further. After potent knockdown of ASNS, cell viability in two doxorubicin-resistant cell lines MES/SA-DxR 2μM and MES/SA-DxR 8μM was decreased, as indicated by an MTT cell proliferation assay. By coupling two-dimensional differential gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry, proteins that play a vital role in ASNS signaling network and development of doxorubicin-induced resistance were identified. Among all the proteins that we have identified, GRP78 and AKR1C1 appear to be involved in drug resistance, replication factor C appears to participate in DNA repairing, and PP6C is proposed to play a role in cell cycle arrest.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"5 3","pages":"Pages 100-102"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2013.07.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78179310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Characterization of a colorectal cancer migration and autophagy-related microRNA miR-338-5p and its target gene PIK3C3 结直肠癌迁移和自噬相关microRNA miR-338-5p及其靶基因PIK3C3的表征

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.bgm.2013.07.006

Jian-An Ju , Ching-Tang Huang , Sheng-Hui Lan , Ting-Huei Wang , Peng-Chan Lin , Jheng-Chang Lee , Yu-Feng Tian , Hsiao-Sheng Liu

{"title":"Characterization of a colorectal cancer migration and autophagy-related microRNA miR-338-5p and its target gene PIK3C3","authors":"Jian-An Ju , Ching-Tang Huang , Sheng-Hui Lan , Ting-Huei Wang , Peng-Chan Lin , Jheng-Chang Lee , Yu-Feng Tian , Hsiao-Sheng Liu","doi":"10.1016/j.bgm.2013.07.006","DOIUrl":"10.1016/j.bgm.2013.07.006","url":null,"abstract":"<div><p>Colorectal cancer (CRC) has a high metastasis rate. MicroRNA (miRNA) is an epigenetic factor required to regulate cell proliferation, tumor cell growth, cancer formation, and metastasis by regulating tumor-suppressor genes or oncogenes. The objective of this study is to identify miRNAs and their target genes related to CRC migration and metastasis. Previously, we used miRNA microarray to reveal that miR-338-5p was significantly upregulated in patients with recurrent CRC. The expression level of miR-338-5p in tumor tissues of metastatic patients is higher than that in nonmetastatic patients. In this study, we report that miR-338-5p expression level was positively correlated with high migration activity of CRC cells. Overexpression of miR-338-5p induced the migration of CRC HCT-116 cells. Furthermore, overexpression of miR-338-5p inhibited its target gene phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3) messenger RNA (mRNA) and protein expression in HCT-116 cells. Downregulation of miR-338-5p increased PIK3C3 mRNA and protein expression in CRC SW480 cells. Furthermore, our study results show that miR-338-5p blocked autophagy, as demonstrated by decreased LC3 type II expression. Autophagy inhibited the migration of colon cancer cell HCT-116 after rapamycin treatment. It can thus be concluded that miR-338-5p induces CRC cell migration by suppressing PIK3C3 expression and autophagy.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"5 3","pages":"Pages 74-78"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2013.07.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78328371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Rapid detection of gene expression by a colorectal cancer Enzymatic Gene Chip Detection Kit 结直肠癌酶促基因芯片检测试剂盒快速检测基因表达

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.gmbhs.2013.05.004

Chan-Han Wu , Fu-Yen Chung , Jia-Yuan Chang , Jaw-Yuan Wang

{"title":"Rapid detection of gene expression by a colorectal cancer Enzymatic Gene Chip Detection Kit","authors":"Chan-Han Wu , Fu-Yen Chung , Jia-Yuan Chang , Jaw-Yuan Wang","doi":"10.1016/j.gmbhs.2013.05.004","DOIUrl":"10.1016/j.gmbhs.2013.05.004","url":null,"abstract":"<div><p>A weighted enzymatic chip array (WEnCA) can detect the gene expression of circulating tumor cells (CTCs) in the peripheral blood of patients with colorectal cancer (CRC). In this study, we used a reagent kit, the GeneCling CRC Enzymatic Gene Chip Detection Kit, which was specifically developed for the WEnCA operation platform to analyze the expression of 31 CRC-related genes of CTCs in the peripheral blood of 30 CRC patients. We moreover evaluated the expression of the genes by simultaneously using real-time quantitative polymerase chain reaction (RT-QPCR). The results showed the overexpression rate of nine genes—<em>DVL1</em>, <em>ELAVL4</em>, <em>CHRNB1</em>, <em>UBE2C</em>, <em>PSAT1</em>, <em>CEA</em>, <em>PTTG1</em>, <em>KRT19</em>, and <em>hTERT</em>—was greater than 90% in the CRC patients. This is concordant with the results of the original WEnCA operation procedure. Linear regression analysis demonstrated a high correlation (<em>r</em> = 0.901; <em>p</em> < 0.0001) between the experimental data of the detection kit and RT-QPCR. The GeneCling CRC Enzymatic Gene Chip Detection Kit is easy, fast, and convenient to operate for detecting gene expression of CTCs from peripheral blood. However, the correlation between the differential expression and the clinicopathological features of the 31 CRC-related genes needs further investigation to verify the clinical value.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"5 3","pages":"Pages 87-91"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2013.05.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74216342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Identification of nilotinib-altered microRNA expression patterns in imatinib-resistant chronic myeloid leukemia cells 尼洛替尼改变的microRNA表达模式在伊马替尼耐药慢性髓性白血病细胞中的鉴定

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.bgm.2013.07.002

Ren-In You , Ching-Liang Ho , Hsiu-Man Hung , Tsu-Yi Chao

{"title":"Identification of nilotinib-altered microRNA expression patterns in imatinib-resistant chronic myeloid leukemia cells","authors":"Ren-In You , Ching-Liang Ho , Hsiu-Man Hung , Tsu-Yi Chao","doi":"10.1016/j.bgm.2013.07.002","DOIUrl":"10.1016/j.bgm.2013.07.002","url":null,"abstract":"<div><p>Drug resistance is a key contributor for treatment failure in hematologic and other malignancies. Nilotinib has been observed with significant activity in patients with chronic phase chronic myeloid leukemia (CML) who have failed or are intolerant of imatinib therapy. Recent reports have suggested that microRNA (miRNA) expression changes are involved in the drug response of human cancer. Several miRNAs have been previously found to be consistently deregulated in imatinib resistance; however, very limited information is available for nilotinib-treated patients who have failed imatinib therapy. Many reports have discovered circulating miRNAs as noninvasive biomarkers of disease and therapy response. The aim of this study was to explore miRNA regulation and find candidate miRNA markers for CML that can be used for drug response prediction. Here we demonstrate the circulating miRNA profile in the culture supernatant of imatinib-resistant K562 CML cells (K562-R) by microarray chip analysis. We have identified specific miRNAs that are associated with nilotinib sensitivity by comparison of miRNA expression patterns from the culture supernatant of nilotinib-treated K562-R cells with the culture supernatant of untreated K562-R cells. The information obtained from this study may have the potential to become a novel biomarker to predict drug response in the future and can also be applied to developing novel therapeutic treatment for CML.</p></div>","PeriodicalId":100178,"journal":{"name":"Biomarkers and Genomic Medicine","volume":"5 3","pages":"Pages 71-73"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bgm.2013.07.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74445166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Rap-1A is a poor prognosis factor in oral squamous cell carcinoma and its expression promotes tumor cell motility via Aurora-A modulation Rap-1A是口腔鳞状细胞癌的不良预后因子，其表达通过Aurora-A调节促进肿瘤细胞运动

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.bgm.2013.08.032

Hsin-Ting Tsai , Chang-Han Chen

引用次数: 0

The proliferative inhibitor and apoptosis mechanism of Linalool in breast cancer cells 芳樟醇在乳腺癌细胞中的增殖抑制作用及其凋亡机制

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.bgm.2013.08.016

Yi-Ling Shen, Ting-Yin Wang, Ting-Yu Chen, Mei-Yin Chang

引用次数: 3

Dysregulation of fibulin-5 correlates with poor prognosis of nasopharyngeal carcinoma and promotes the metastasis of nasopharyngeal cells through the AKT signaling pathway 纤维蛋白-5异常与鼻咽癌预后不良相关，并通过AKT信号通路促进鼻咽癌细胞转移

Biomarkers and Genomic Medicine Pub Date : 2013-09-01 DOI: 10.1016/j.bgm.2013.08.030

Hsin-Ting Tsai , Chang-Han Chen

引用次数: 0