Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects最新文献_第9页

Transients in glycolytic metabolism following electrical activity in electrophorus electrophus电活动后糖酵解代谢的瞬变

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-07-29 DOI: 10.1016/0926-6577(64)90159-7

P.K. Maitra, A. Ghosh, B. Schoener, B. Chance

{"title":"Transients in glycolytic metabolism following electrical activity in electrophorus","authors":"P.K. Maitra, A. Ghosh, B. Schoener, B. Chance","doi":"10.1016/0926-6577(64)90159-7","DOIUrl":"10.1016/0926-6577(64)90159-7","url":null,"abstract":"<div><p>The cyclic increases and decreases of fluorescence emission of slices of the main organ of <em>Electrophorus electricus</em> which have been interpreted as DPN<sup>+</sup> reduction at the glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) site are here correlated with assays of essential intermediates of glycolysis. Large increases of fructose 1,6-diphosphate, glyceraldehyde 3-phosphate, 3-phosphoglyceric acid and 2-phospholgyceric acid are found to occur at the time of the maximum increases of fluorescence and to have subsided as the flourescence increase subsides. Measurements of increased DPN concentration and glyceraldehyde 3-phosphate concentration identify the fluorescence increase with DPN<sup>+</sup> reduction at glyceraldehyde-3-phosphate dehydrogenase. The activation of glycolysis appears to be due to a concentrated effect of increased ADP and creased ATP concentration upon phosphofructokinase (EC 2.7.1.11), 1,3-diphosphojlycerate kinase (EC 2.7.2.3) and glyceraldehyde-3-phosphate dehydrogenase. The possibility of other activators of phosphofructokinase is also to be considered.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 1","pages":"Pages 112-119"},"PeriodicalIF":0.0,"publicationDate":"1964-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90159-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40765809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Anomalous diffraction spectra of biological cells 生物细胞的异常衍射光谱

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-07-29 DOI: 10.1016/0926-6577(64)90165-2

Tamako Kurozumi , Kazuo Shibata

{"title":"Anomalous diffraction spectra of biological cells","authors":"Tamako Kurozumi , Kazuo Shibata","doi":"10.1016/0926-6577(64)90165-2","DOIUrl":"10.1016/0926-6577(64)90165-2","url":null,"abstract":"<div><p>Anomalous diffraction spectra of monolayers of biological cells dried on a quartz plate were observed between 0.25 and 2.5 μ. The possibility of using the positions of the diffraction maxima and minima for the determination of cell dimensions was examined on the basis of a simple theory of anomalous diffraction for spherical or discoidal cells. In the spectra region used, yeast and Chlorella cells showed two diffraction maxima, and spinach chloroplasts and human erythrocytes a single maximum. The relative wavelengths of the two maxima and the minimum between them were in the ratios calculated from the theory, so that it was possible to evaluate a term proportional to the cell diameter or thickness. The proportionality between the evaluated quantity and the diameter was examined with yeast, Chlorella cells, cloroplasts, and erythrocytes, and was approximately constant. A semi-empirical formula for the determination of cell dimensions was obtained by assuming the average of the proportionality constants for these different species of cells. The maximum error due to this assumption and due to the variation of the content of solid materials in cells was estimated to be 13% in diameter. The diffraction method was compared with other optical or microscopic methods for the determination of cell dimensions.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 1","pages":"Pages 191-200"},"PeriodicalIF":0.0,"publicationDate":"1964-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90165-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40765816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Action spectra of the reducing and oxidizing systems in spinach chloroplasts 菠菜叶绿体中还原和氧化系统的作用谱

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-07-29 DOI: 10.1016/0926-6577(64)90152-4

H. Lundegårdh

{"title":"Action spectra of the reducing and oxidizing systems in spinach chloroplasts","authors":"H. Lundegårdh","doi":"10.1016/0926-6577(64)90152-4","DOIUrl":"10.1016/0926-6577(64)90152-4","url":null,"abstract":"<div><p>The reducing moiety of the photosynthetic cycle, resulting in TPN<sup>+</sup> reduction, and the oxidizing moiety (cytochromes), resulting in the main part of ATP production, were excited by separate light reactions and showed different action spectra. The two light reactions were also characterized by specific changes in the absorption spectra of the pigments. The reducing system was more sensitive to blue-green light, oxidized carotenoids (negative band at 500 mμ) and reduced an initial reductant. The oxidizing system was specifically sensitive to red light, oxidized the cytochrome system and reduced the pigments via chlorophyll and unknown factors showing negative bands at 640 mμ. The action spectra showed a multilateral linkage between pigments and enzyme systems and considerable activity also in green at higher light intensities. It is suggested that the enzyme systems together form a balancing steady state, which operates also in the dark and is sensitive to variations in the concentrations of O<sub>2</sub> and various substrates. The site of O<sub>2</sub>-production (photolysis of H<sub>2</sub>O) is probably at the junction of th two redox systems, <em>viz.</em> at the autoxidable cytochrome, <em>b</em><sub>3</sub> and flavoprotein. Its action spectrum showed characteristics of both the reducing and oxidizing systems. The action spectra are influenced by light scattering.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 1","pages":"Pages 37-56"},"PeriodicalIF":0.0,"publicationDate":"1964-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90152-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75691631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Iron-containing proteins in euglena 真核藻中的含铁蛋白质

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-07-29 DOI: 10.1016/0926-6577(64)90156-1

Fulvio Perini , Jerome A. Schiff, Martin D. Kamen

引用次数: 23

Flow birefringence of a mixture of deoxyribonucleic acid and actomyosin 脱氧核糖核酸和肌动球蛋白混合物的流动双折射

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-07-29 DOI: 10.1016/0926-6577(64)90172-X

Koscak Maruyama, Takashi Obinata

引用次数: 0

Improvement of low-temperature absorption spectra by use of an on-line computer 利用联机计算机改进低温吸收光谱

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-07-29 DOI: 10.1016/0926-6577(64)90173-1

Williard B. Elliott , Richard E. Hayford , William Tanski

引用次数: 10

Extracellular pathways in the peripheral nerve fibres: Schwann-cell-layer permeability to thorium dioxide 周围神经纤维的细胞外通路:雪旺细胞层对二氧化钍的渗透性

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-07-29 DOI: 10.1016/0926-6577(64)90177-9

Gloria M. Villegas, Raimundo Villegas

引用次数: 20

Hill activity in cell-free preparations of a blue-green alga 蓝绿藻无细胞制备中的Hill活性

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-07-29 DOI: 10.1016/0926-6577(64)90150-0

Walter A. Susor, David W. Krogmann

引用次数: 62

Light stimulation of the respiratory activity of Rhodospirillum rubrum chromatophores 红红螺旋藻色素体呼吸活动的光刺激

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-05-25 DOI: 10.1016/0926-6577(64)90210-4

Goro Kikuchi, Hiroshi Yamada , Hiroshi Sato

{"title":"Light stimulation of the respiratory activity of Rhodospirillum rubrum chromatophores","authors":"Goro Kikuchi, Hiroshi Yamada , Hiroshi Sato","doi":"10.1016/0926-6577(64)90210-4","DOIUrl":"10.1016/0926-6577(64)90210-4","url":null,"abstract":"<div><p>Respiratory activity of chromatophores of <em>Rhodospirillum rubrum</em>, as measured by the O<sub>2</sub> uptake, was almost doubled by illumination. Succinoxidase activity of chromatophores was inhibited considerably by o-phenanthroline but not inhibited by KCN either in the dark or light. The aerobic oxidation of p-phenylenediamine in the dark was not inhibited by o-phenanthroline but was completely inhibited by KCN, whereas the oxidation in the light was sensitive to o-phenanthroline but insensitive to KCN. o-Phenanthroline also inhibited other CN<sup>−</sup>-insensitive reactions in chromatophores, such as photooxidation of reduced cytochrome c<sub>2</sub> and the dark-anaerobic oxidation of NADH coupled to the reduction of fumarate. Antimycin A did not inhibit the succinoxidase activity in the dark, but significantly inhibited the activity in the light. Succinate-cytochrome c<sub>2</sub> reductase activity was sensitive to antimycin A but insensitive to o-phenanthroline, whereas NADH-cytochrome c<sub>2</sub> reductase activity was insensitive to antimycin A but sensitive to o-phenanthroline.</p><p>The terminal oxidation system in chromatophores is discussed with particular reference to the marked stimulation of O<sub>2</sub> uptake by illumination.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"79 3","pages":"Pages 446-455"},"PeriodicalIF":0.0,"publicationDate":"1964-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90210-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23761996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Molecular orientation in quantasomes II. Absorption spectra, hill activity and fluorescence yields 量子体的分子取向2。吸收光谱、山活性和荧光产率

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-05-25 DOI: 10.1016/0926-6577(64)90213-X

Kenneth Sauer, Roderic B. Park

{"title":"Molecular orientation in quantasomes II. Absorption spectra, hill activity and fluorescence yields","authors":"Kenneth Sauer, Roderic B. Park","doi":"10.1016/0926-6577(64)90213-X","DOIUrl":"10.1016/0926-6577(64)90213-X","url":null,"abstract":"<div><p>The absorption spectrum of spinach quantasomes is compared with those of whole chloroplasts and intact leaves. Using the scattered-transmission technique, it is shown that the absorption maximum at 678.5 mμ in spinach leaves is not shifted in the preparation of isolated chloroplasts or by sonication and separation of small quantasome aggregates.</p><p>The effects of several organic compounds and surface-active agents on the absorption spectra, chlorophyll fluorescence and Hill-reaction activity of quantasomes fall into two general classes. Added acetone, methanol, urea or sodium dodecyl sulfate induce a loss in Hill activity at appreciably lower concentrations than are required to give a pronounced fluorescence increase or blue shift in the absorption maximum at 678 mμ. On the other hand, quantasomes treated with Triton X-100 retain Hill activity at higher concentrations of the non-ionic detergent than are necessary to produce pronounced absorption and fluorescence changes. The principal conclusions reached are: (1) the position of the chlorophyll maximum in the red is not a sensitive measure of the ability of photosynthetic materials to carry out the Hill reaction, (2) energy diverted from normal photochemical pathways does not in general immediately appear as enhanced fluorescence, and (3) detergent concentrations comparable to the chlorophyll concentrations of quantasome aggregates are capable of profound effects on physical properties.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"79 3","pages":"Pages 476-489"},"PeriodicalIF":0.0,"publicationDate":"1964-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90213-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23761999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20