Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis最新文献_第9页

DNAase I digestion of early, middle, and late S phase replicating DNA in murine leukemia L5178Y cells 小鼠白血病L5178Y细胞早期、中期和晚期S期复制DNA的DNA酶I消化

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90074-5

T. Ono, S. Okada

引用次数: 4

In vivo DNA degradation in thymocytes of γ-irradiated or hydrocortisone-treated rats γ辐照或氢化可的松处理大鼠胸腺细胞的体内DNA降解

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90060-5

S.R. Umansky, B.A. Korol', P.A. Nelipovich

{"title":"In vivo DNA degradation in thymocytes of γ-irradiated or hydrocortisone-treated rats","authors":"S.R. Umansky, B.A. Korol', P.A. Nelipovich","doi":"10.1016/0005-2787(81)90060-5","DOIUrl":"10.1016/0005-2787(81)90060-5","url":null,"abstract":"<div>Gamma-irradiation or introduction of hydrocortisone bring about degradation of nuclear DNA in rat thymocytes. The chromatin degradation products were extracted from purified nuclei by 0.7 mM EDTA. The quantity of low molecular weight chromatin fragments formed 6 h after irradiation increases up to the doses of 3 Gy, then remains constant up to 30 Gy and decreases at doses 100 to 300 Gy. Whatever the irradiation dose, DNA degradation starts after a 2-h lag, reaches a maximum by the 6th hour and remains constant between the 6th and 10th hours. The quantity of chromatin fragments formed coincides with the number of cells with pycnotic nuclei. The chromatin fragments present nucleosomes and their oligomers with a normal histone content and an intact structure, as judged from how they are split by DNAase I. The number of intranucleosomal breaks in DNA is negligible. DNA fragmentation is not accompanied by degradation of histones and nonhistone proteins of chromatin. Hence, DNAase I and proteases are not involved in degradation of chromatin. The ratio between mononucleosomes and oligomeres of different lengths does not depend on the dose and the time after irradiation. The quantity of DNA degraded is determined by the number of dying cells in which all DNA is fragmented rather than the degree of chromatin degradation over the whole thymocyte population. Hydrocortisone-induced degradation of chromatin in rat thymocytes occurs similarly. A possible role of chromatin degradation in cell death is discussed.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"655 1","pages":"Pages 9-17"},"PeriodicalIF":0.0,"publicationDate":"1981-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90060-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18273604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 142

The development of a radioimmunoassay for the detection of photoproducts in mammalian cell DNA 用于检测哺乳动物细胞DNA中光产物的放射免疫测定法的发展

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90066-6

David L. Mitchell, Judith M. Clarkson

{"title":"The development of a radioimmunoassay for the detection of photoproducts in mammalian cell DNA","authors":"David L. Mitchell, Judith M. Clarkson","doi":"10.1016/0005-2787(81)90066-6","DOIUrl":"10.1016/0005-2787(81)90066-6","url":null,"abstract":"<div>Antiserum was prepared in rabbits against ultraviolet-irradiated DNA. Using a 125I-labeled protein A binding assay it was shown to be specific for ultraviolet-irradiated DNA, binding increasing as a function of logarithmic increase in dose. This antiserum was then used to develop a radioimmunoassay in which the competition between labeled UV-DNA and unlabeled sample DNA for antibody binding sites is monitored. Using this system the specificity of the assay could be changed depending on the nature of the labeled probe. The inability of poly(dA-dT) · poly(dA-dT), as compared with poly(dA) · poly(dT), to act as a competitive inhibitor established that the primary lesion recognized by the antiserum is the thymine dimer. This antigenic response did, however, depend on the presence of at least one nucleotide adjacent to the dimer. The sensitivity of the assay was optimized by using 32P-labeled plasmid DNA as competitive probe and is capable of detecting photodamage in cellular DNA at doses as low as 2.5 J · m−2.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"655 1","pages":"Pages 54-60"},"PeriodicalIF":0.0,"publicationDate":"1981-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90066-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18273602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 73

Inhibition of the action of Escherichia coli transcription termination protein ϱ by poly(C) and heparin 聚(C)和肝素对大肠杆菌转录终止蛋白ϱ的抑制作用

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90071-X

Susan L. Bektesh , John P. Richardson

引用次数: 1

Titles of related papers in other sections 其他章节相关论文的标题

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90075-7

引用次数: 0

DNA repair enzyme deficiency and in vitro complementation of the enzyme activity in cell-free extracts from ataxia telangiectasia fibroblasts 共济失调毛细血管扩张成纤维细胞无细胞提取物DNA修复酶缺乏及酶活性的体外补充

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90065-4

Tadashi Inoue, Akiko Yokoiyama, Tsuneo Kada

引用次数: 25

Inhibition of tyrosine aminotransferase induction by UTP deficiency and its reversal by 5-fluorouridine in cultured hepatoma cells UTP缺乏对培养肝癌细胞酪氨酸转氨酶诱导的抑制及5-氟吡啶的逆转作用

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90063-0

Eva M. Giesen , Gisèle Beck , Axel Holstege , Dietrich O.R. Keppler

引用次数: 2

The synthesis of ribosomal 5 S RNA in cultured hamster cells during the inhibition of protein synthesis 抑制蛋白质合成过程中培养的仓鼠细胞核糖体5s RNA的合成

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90062-9

Kenji Takenaka , Mikio Yamamoto , Hideya Endo , Michihiko Kuwano

{"title":"The synthesis of ribosomal 5 S RNA in cultured hamster cells during the inhibition of protein synthesis","authors":"Kenji Takenaka , Mikio Yamamoto , Hideya Endo , Michihiko Kuwano","doi":"10.1016/0005-2787(81)90062-9","DOIUrl":"10.1016/0005-2787(81)90062-9","url":null,"abstract":"<div>Ribosomal 5 S RNA synthesis in Chinese hamster V79 cells treated with an inhibitory antibiotic of protein synthesis, cycloheximide, was quantitated by hybridization of RNA preparations with plasmid ColE1-pSC101 DNA carrying Xenopus 5 S DNA. In V79 cells, cycloheximide produced a notable decrease in the production of the higher molecular weight ribosomal RNA (rRNA) fraction, whereas the accumulation of both 5 S RNA and polyadenylate-containing messenger RNA was much less affected. When the cellular protein synthesis was inhibited by over 85% of the control, accumulation of 5 S RNA and of rRNA was respectively 40–50% and about 10% of the control. The size distribution analysis of RNA species revealed that 5 S RNAs obtained from V79 cells after treatment with 0.1 μg/ml or 2.0 μg/ml cycloheximide were indistinguishable from control. Inhibition of precursor uptake into 5 S RNA by cycloheximide was apparently phase-specific during the cell cycle and was 44–67% and 78–85% of the control for the S and the M phase, respectively. The responses of RNA polymerase III activities to cycloheximide in isolated nuclei from each phase of the cell cycle correlated very well with those of precursor uptake into 5 S RNA observed in vivo, while total solubilized RNA polymerase activities showed no inhibition by the drug at any phase of the cell cycle. Cytosine arabinoside, a specific inhibitor of DNA synthesis, did not cause any decrease in the cellular level of 5 S RNA.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"655 1","pages":"Pages 26-33"},"PeriodicalIF":0.0,"publicationDate":"1981-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90062-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18069774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Conformational changes of yeast tRNAPhe upon interaction with intercalators probed by nuclease digestion 酵母tRNAPhe与核酸酶酶切探针插入物相互作用时的构象变化

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90070-8

Peter Eigil Nielsen

引用次数: 5

Overproduction of the EcoRII endonuclease and methylase by Escherichia coli strains carrying recombinant plasmids constructed in vitro 体外构建重组质粒的大肠杆菌菌株过量生产EcoRII核酸内切酶和甲基化酶

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-08-27 DOI: 10.1016/0005-2787(81)90072-1

V.G. Kosykh, A.S. Solonin, Ya.I. Buryanov, A.A. Bayev

{"title":"Overproduction of the EcoRII endonuclease and methylase by Escherichia coli strains carrying recombinant plasmids constructed in vitro","authors":"V.G. Kosykh, A.S. Solonin, Ya.I. Buryanov, A.A. Bayev","doi":"10.1016/0005-2787(81)90072-1","DOIUrl":"10.1016/0005-2787(81)90072-1","url":null,"abstract":"<div>Recombinant DNA molecules were constructed from the plasmid pIL203 and the EcoRI-fragment of N3 plasmid containing EcoRII endonuclease and methylase genes and also a gene for resistance to sulfanilamide. The pIL203 plasmid, used as a vector, consisted of the BamHI-EcoRI-fragment of the plasmid pBR322 conferring resistance to ampicillin and the BamHI-EcoRI-fragment of lambda phage containing promoters, a thermosensitive mutation in the cI gene and a suppressible amber mutation in the cro gene. Ampicillin-sulfanilamide-resistant clones were selected and tested for their restriction and modification phenotype. The recombinant plasmid DNA, isolated from ApRSuR-resistant clones, which restricted and modified phage λ imm21 with EcoRII specificity, had the EcoRI-fragment with EcoRII genes in a single orientation. The recombinant plasmid pSK323 was transferred into E. coli strains with su−, su1, su2 or su3 phenotypes. The synthesis of products of EcoRII genes by these strains grown at 37°C is increased by 10–50-fold.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"655 1","pages":"Pages 102-106"},"PeriodicalIF":0.0,"publicationDate":"1981-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90072-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17328700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10