Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism最新文献_第2页

The acute phase response in apolipoprotein A-1 knockout mice: apolipoprotein serum amyloid A and lipid distribution in plasma high density lipoproteins 载脂蛋白A-1基因敲除小鼠急性期反应:载脂蛋白血清淀粉样蛋白A和血浆高密度脂蛋白脂质分布

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-11-02 DOI: 10.1016/S0005-2760(98)00109-X

Tahar Hajri , Rosemary Elliott-Bryant , Jean D. Sipe , Jun-S Liang , K.C. Hayes , Edgar S. Cathcart

{"title":"The acute phase response in apolipoprotein A-1 knockout mice: apolipoprotein serum amyloid A and lipid distribution in plasma high density lipoproteins","authors":"Tahar Hajri , Rosemary Elliott-Bryant , Jean D. Sipe , Jun-S Liang , K.C. Hayes , Edgar S. Cathcart","doi":"10.1016/S0005-2760(98)00109-X","DOIUrl":"10.1016/S0005-2760(98)00109-X","url":null,"abstract":"<div>In plasma, the bulk of apoSAA, a positive acute phase reactant protein, is transported in high density lipoproteins (HDL), especially HDLH (apoA1-rich HDL). In this study we tested whether apoA1 deficiency would adversely affect apoSAA concentration and lipid distribution in mouse plasma lipoproteins. Acute phase response (APR) was induced in C57BL/6J (apoA1+/+) and apoA1-knockout mice (apoA1−/−) by a subcutaneous injection of silver nitrate. The APR increased cholesterol concentrations in LDL of apoA1−/− mice and apoA1+/+ mice in a like manner. In contrast to apoA1+/+ mice, concentrations of cholesterol, phospholipids and proteins in both HDLL (1.063<d<1.103 g/ml) and HDLH (1.103<d<1.21 g/ml) were significantly increased by the APR in apoA1−/− mice. Total concentration of plasma apoSAA and its distribution in lipoprotein fractions was similar in both APR groups. The bulk of plasma apoSAA was contained in HDL and not in VLDL or LDL even when the HDL concentration was low. In apoA1−/− mice, HDLL and HDLH contained more apoSAA than in apoA1+/+ mice. These results indicate that apoA1−/− mice are not deterred from mounting an apoSAA response similar to apoA1+/+ mice and that apoA1-rich HDL particles are not necessary for apoSAA transport in the plasma.</div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00109-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20707610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Fatty acid α-oxidation of tetradecylthioacetic acid and tetradecylthiopropionic acid in cucumber (Cucumis sativus) 黄瓜(Cucumis sativus)中脂肪酸α-氧化十四烷基硫代乙酸和十四烷基硫代丙酸

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-11-02 DOI: 10.1016/S0005-2760(98)00113-1

Grethe I Andersen Borge , Erik Slinde , Astrid Nilsson

{"title":"Fatty acid α-oxidation of tetradecylthioacetic acid and tetradecylthiopropionic acid in cucumber (Cucumis sativus)","authors":"Grethe I Andersen Borge , Erik Slinde , Astrid Nilsson","doi":"10.1016/S0005-2760(98)00113-1","DOIUrl":"10.1016/S0005-2760(98)00113-1","url":null,"abstract":"<div>Fatty acid α-oxidation in cucumber (Cucumis sativus) involves enzymatic conversion of long-chain Cn-fatty acids to the C(n−1)-aldehyde and CO2. However, the mechanism of this process is not well understood. In this study, the α-oxidation of the fatty acid analogues tetradecylthioacetic acid (TTA) and tetradecylthiopropionic acid (TTP) with a sulphur atom substituting the methylene group in positions 3 and 4, respectively, was investigated and compared to palmitic acid. Both [1-14C]TTA and [1-14C]TTP could be α-oxidised in the cucumber subcellular 150 000×gmax fraction. [1-14C]TTP was an even better substrate compared to the natural palmitic acid, while [1-14C]TTA was α-oxidised to a lower extent. [2-14C]TTA revealed no 14CO2, indicating that only one cycle of α-oxidation occurred. TTA was an inhibitor of the palmitic acid α-oxidation, and the inhibitory effects were examined.</div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00113-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20706618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Characterisation of triacylglycerol hydrolase activities in human placenta 人胎盘三酰甘油水解酶活性的表征

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-11-02 DOI: 10.1016/S0005-2760(98)00105-2

Ian J. Waterman , Neil Emmison , Asim K. Dutta-Roy

引用次数: 50

Biosynthesis and possible biological functions of plasmalogens 缩醛原的生物合成及其可能的生物学功能

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-11-02 DOI: 10.1016/S0005-2760(98)00107-6

Ten-ching Lee

引用次数: 108

Effect of β-cyclodextrin dietary supplementation on biliary proteins and their resulting cholesterol nucleating activity in pigs 饲粮中添加β-环糊精对猪胆道蛋白及其胆固醇成核活性的影响

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-10-02 DOI: 10.1016/S0005-2760(98)00101-5

Isabelle Catala , Nicole Domingo , Catherine Juste , Anne-Marie Gueugneau , Bernard Thorin , Claude Lutton , Tristan Corring , Huguette Lafont

{"title":"Effect of β-cyclodextrin dietary supplementation on biliary proteins and their resulting cholesterol nucleating activity in pigs","authors":"Isabelle Catala , Nicole Domingo , Catherine Juste , Anne-Marie Gueugneau , Bernard Thorin , Claude Lutton , Tristan Corring , Huguette Lafont","doi":"10.1016/S0005-2760(98)00101-5","DOIUrl":"10.1016/S0005-2760(98)00101-5","url":null,"abstract":"<div>We explored the possibility that the biliary protein fraction may support part of the variation in the nucleating activity previously measured in gallbladder biles of pigs. Eighteen gallbladder aspirates freshly obtained from three dietary groups (0, 5, or 10% β-cyclodextrin) of six pigs were chromatographed to purify their total protein fraction. Proteins were quantified, and analysed through electrophoresis and immunoblotting or enzyme-linked immunosorbent assay for albumin, and five putative effectors of cholesterol crystallisation, mucins, immunoglobulin A, 130 kDa, apolipoprotein A-I, and anionic polypeptide fraction. Each total protein fraction was also assayed for its ability to influence cholesterol precipitation, when added to supersaturated model bile. The current data provided evidence that the cholesterol crystallisation-promoting activity of biliary proteins in model biles increased with the β-cyclodextrin dietary content. This occurred without any significant change in the total biliary protein content, but was associated with a significant decrease in the concentration of albumin and apolipoprotein A-I, resulting in changes in the overall balance of proteins in bile. Comparison of these results with the crystallisation figures previously obtained from the corresponding native biles led us to conclude that biliary proteins might influence the outcome of the crystallisation process, namely the final crystal concentration at equilibrium, but would not systematically represent a major driving force for determining the velocity of crystal formation in native bile of pigs.</div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00101-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20679667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A unique amyloidogenic apolipoprotein serum amyloid A (apoSAA) isoform expressed by the amyloid resistant CE/J mouse strain exhibits higher affinity for macrophages than apoSAA1 and apoSAA2 expressed by amyloid susceptible CBA/J mice 抗淀粉样蛋白CE/J小鼠菌株表达的一种独特的淀粉样蛋白载脂蛋白血清淀粉样蛋白A (apoSAA)异构体对巨噬细胞的亲和力高于淀粉样蛋白易感CBA/J小鼠表达的apoSAA1和apoSAA2

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-10-02 DOI: 10.1016/S0005-2760(98)00102-7

Jun-shan Liang , Rosemary Elliott-Bryant , Tahar Hajri , Jean D. Sipe , Edgar S. Cathcart

{"title":"A unique amyloidogenic apolipoprotein serum amyloid A (apoSAA) isoform expressed by the amyloid resistant CE/J mouse strain exhibits higher affinity for macrophages than apoSAA1 and apoSAA2 expressed by amyloid susceptible CBA/J mice","authors":"Jun-shan Liang , Rosemary Elliott-Bryant , Tahar Hajri , Jean D. Sipe , Edgar S. Cathcart","doi":"10.1016/S0005-2760(98)00102-7","DOIUrl":"10.1016/S0005-2760(98)00102-7","url":null,"abstract":"<div>CBA/J and other inbred strains of mice that express the amyloidogenic apolipoprotein serum amyloid A (apoSAA) apoSAA2, together with apoSAA1, are susceptible to amyloid A (AA) amyloidosis, whereas CE/J mice that express a single unique isoform, apoSAACEJ, are resistant. Studies indicate that CBA/J×CE/J hybrid mice that express apoSAA2 in the presence of apoSAACEJ are protected from amyloidogenesis. To define a mechanism by which expression of apoSAACEJ may protect from AA formation in the presence of apoSAA2, binding of recombinant apoSAA (r-apoSAA) isoforms, validated by N-terminal sequencing, to a murine macrophage cell line was investigated. Maximal specific binding occurred after incubation of radiolabeled apoSAA with IC-21 macrophages (1×105 cells/ml) for 30 min at 4°C. The binding of 125I-r-apoSAA1, 125I-r-apoSAA2 and 125I-r-apoSAACEJ was specific and saturable, with an affinity (Kd) of about 2.8, 3.2 and 1.3 nM, respectively, and approximately 2–4×106 sites per cell. Competitive binding experiments indicate apoSAACEJ binds with higher affinity to macrophages than does either apoSAA1 or apoSAA2. We suggest that greater cellular affinity of apoSAACEJ compared to apoSAA2 may contribute to protection from AA amyloid in certain CBA/J×CE/J hybrid mice by interfering with interaction of apoSAA2 by macrophages and hence either membrane associated or intracellular degradation.</div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00102-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20680716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Incorporation of extracellular phospholipids and their effect on the growth and lipid metabolism of the Saccharomyces cerevisiae cho1/pss mutant 胞外磷脂的掺入及其对酿酒酵母cho1/pss突变体生长和脂质代谢的影响

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-10-02 DOI: 10.1016/S0005-2760(98)00092-7

Jei-Oh Yon, Hidemitsu Nakamura, Akinori Ohta, Masamichi Takagi

{"title":"Incorporation of extracellular phospholipids and their effect on the growth and lipid metabolism of the Saccharomyces cerevisiae cho1/pss mutant","authors":"Jei-Oh Yon, Hidemitsu Nakamura, Akinori Ohta, Masamichi Takagi","doi":"10.1016/S0005-2760(98)00092-7","DOIUrl":"10.1016/S0005-2760(98)00092-7","url":null,"abstract":"<div>The cho1/pss mutant of Saccharomyces cerevisiae, which is auxotrophic for choline or ethanolamine because of the deficiency in phosphatidylserine synthesis, grew in the presence of 0.05 mM phosphatidylcholine (PC) with octanoic acids (diC8PC) or decanoic acids (diC10PC), but not in the presence of PC with longer acyl residues. It did not grow in the presence of the soluble hydrolytic products of PC, phosphorylcholine or glycerophosphorylcholine, at comparable concentrations. Addition of 10 mM hemicholinium-3, a choline transport inhibitor, or disruption of the CTR gene, which encodes a choline transporter, inhibited the growth of the cho1/pss mutant in the presence of choline, but not in the presence of 0.1 mM diC8PC. Under diC8PC-supported growth conditions, octanoic acid was barely detectable in the cellular phospholipid fraction, but was recovered in the culture medium as the free acid, and the phosphatidylethanolamine (PE) content was low in comparison to the choline-supported conditions. These results suggest that PCs with short acyl residues were taken up by the cho1/pss mutant and remodeled as they were used, and that PCs with short acyl residues do not inhibit conversion of PE to PC. The current results provide a new direction in the analysis of intracellular phospholipid movement and metabolism in yeast.</div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00092-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20679971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Inositol polyphosphates regulate the membrane interactions of the endosomal p100, G-protein-related protein 肌醇多磷酸调节内体p100, g蛋白相关蛋白的膜相互作用

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-10-02 DOI: 10.1016/S0005-2760(98)00099-X

Tatiana A. Kassessinoff , Andrew Gabet , Michael A. Beaven , Ronit Sagi-Eisenberg

{"title":"Inositol polyphosphates regulate the membrane interactions of the endosomal p100, G-protein-related protein","authors":"Tatiana A. Kassessinoff , Andrew Gabet , Michael A. Beaven , Ronit Sagi-Eisenberg","doi":"10.1016/S0005-2760(98)00099-X","DOIUrl":"10.1016/S0005-2760(98)00099-X","url":null,"abstract":"<div>The protein, p100, was previously identified as a G-protein related protein that cycles on and off the cytoplasmic face of the endosome membrane (Traub et al., Biochem. J. 280 (1991) 171–178). Here we present evidence that the inositol polyphosphates, inositol 1,4,5-trisphosphate (IP3) and inositol hexakisphosphate (IP6), release p100 from light-density microsomal membranes and inhibit rebinding of p100 through receptors, which are specific for IP3 or for IP6. These receptors can be co-extracted with p100 from the microsomes by 0.5 M Tris-HCl and, in the soluble state, they exhibit similar binding activity towards the inositol polyphosphates as do untreated microsomes. Soluble p100 self-aggregates and this aggregation is blocked by both IP3 and IP6. Stimulation of permeabilized rat basophilic leukemia (RBL-2H3) cells with carbachol, via transfected muscarinic m1 receptors, results in increased levels of inositol polyphosphates and the quantitative release of p100 into the cytosol. This effect is reversible and cytosolic p100 rebinds to the membrane as the levels of inositol polyphosphates decline. These findings suggest that p100 may belong to a family of IP-binding proteins whose intracellular localization is determined by extracellular signals.</div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00099-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20680713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Structure and expression of fatty acid desaturases 脂肪酸去饱和酶的结构和表达

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-10-02 DOI: 10.1016/S0005-2760(98)00091-5

Dmitry A. Los , Norio Murata

引用次数: 504

Expression and characterization of human group V phospholipase A2 人V组磷脂酶A2的表达与特性研究

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-10-02 DOI: 10.1016/S0005-2760(98)00098-8

Yijun Chen, Edward A. Dennis

{"title":"Expression and characterization of human group V phospholipase A2","authors":"Yijun Chen, Edward A. Dennis","doi":"10.1016/S0005-2760(98)00098-8","DOIUrl":"10.1016/S0005-2760(98)00098-8","url":null,"abstract":"<div>Group V phospholipase A2 (GV-PLA2) has been shown to be involved in signal transduction and inflammatory processes in cellular studies, but the physical and biochemical properties of this important enzyme have been unclear. We report the over-expression and characterization of GV-PLA2. The GV-PLA2 cDNA was synthesized from human heart polyA+ mRNA by RT-PCR, and an expression construct containing the GV-PLA2 was established. After expression in Escherichia coli cells, the protein was solubilized and purified to homogeneity in a single step using nickel affinity chromatography. The purified GV-PLA2 protein was folded to form active enzyme. The recombinant GV-PLA2 has an absolute requirement for Ca2+ for enzymatic activity. The optimum pH for this enzyme is pH 8.5 in Tris-HCl buffer with sonicated vesicles as substrate. GV-PLA2 preferentially hydrolyzes phosphatidylethanolamine (PE) vesicles compared to phosphatidylcholine (PC) vesicles. However, hydrolysis of PC and PE is equivalent in mixed vesicles of the phospholipids. The fatty acid preference of GV-PLA2 is linoleoyl>palmitoyl>arachidonyl with a PC head group and sonicated vesicles. 3-(3-Actamide-1-benzyl-2-ethylindolyl-5-oxy)propane phosphonic acid (LY311727), a potent inhibitor of human group IIA PLA2, strongly inhibits GV-PLA2 with an IC50 value of about 36 nM which is comparable to its inhibition of group IIA PLA2.</div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00098-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20681018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 81