The acute phase response in apolipoprotein A-1 knockout mice: apolipoprotein serum amyloid A and lipid distribution in plasma high density lipoproteins

Tahar Hajri , Rosemary Elliott-Bryant , Jean D. Sipe , Jun-S Liang , K.C. Hayes , Edgar S. Cathcart
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引用次数: 15

Abstract

In plasma, the bulk of apoSAA, a positive acute phase reactant protein, is transported in high density lipoproteins (HDL), especially HDLH (apoA1-rich HDL). In this study we tested whether apoA1 deficiency would adversely affect apoSAA concentration and lipid distribution in mouse plasma lipoproteins. Acute phase response (APR) was induced in C57BL/6J (apoA1+/+) and apoA1-knockout mice (apoA1−/−) by a subcutaneous injection of silver nitrate. The APR increased cholesterol concentrations in LDL of apoA1−/− mice and apoA1+/+ mice in a like manner. In contrast to apoA1+/+ mice, concentrations of cholesterol, phospholipids and proteins in both HDLL (1.063<d<1.103 g/ml) and HDLH (1.103<d<1.21 g/ml) were significantly increased by the APR in apoA1−/− mice. Total concentration of plasma apoSAA and its distribution in lipoprotein fractions was similar in both APR groups. The bulk of plasma apoSAA was contained in HDL and not in VLDL or LDL even when the HDL concentration was low. In apoA1−/− mice, HDLL and HDLH contained more apoSAA than in apoA1+/+ mice. These results indicate that apoA1−/− mice are not deterred from mounting an apoSAA response similar to apoA1+/+ mice and that apoA1-rich HDL particles are not necessary for apoSAA transport in the plasma.

载脂蛋白A-1基因敲除小鼠急性期反应:载脂蛋白血清淀粉样蛋白A和血浆高密度脂蛋白脂质分布
在血浆中,apoSAA(一种急性期阳性反应蛋白)的大部分以高密度脂蛋白(HDL),特别是HDLH(富含apoa1的HDL)的形式运输。在本研究中,我们测试了apoA1缺乏是否会对小鼠血浆脂蛋白中的apoSAA浓度和脂质分布产生不利影响。皮下注射硝酸银诱导C57BL/6J (apoA1+/+)和apoA1敲除小鼠(apoA1−/−)急性期反应(APR)。APR以类似的方式增加apoA1−/−小鼠和apoA1+/+小鼠LDL中的胆固醇浓度。与apoA1+/+小鼠相比,APR使apoA1−/−小鼠的hdl (1.063<d<1.103 g/ml)和HDLH (1.103<d<1.21 g/ml)中的胆固醇、磷脂和蛋白质浓度显著升高。APR组血浆apoSAA总浓度及其在脂蛋白组分中的分布相似。血浆apoSAA大部分存在于HDL中,而不存在于VLDL或LDL中,即使HDL浓度较低。在apoA1−/−小鼠中,HDLL和HDLH比apoA1+/+小鼠含有更多的apoSAA。这些结果表明,apoA1−/−小鼠不会像apoA1+/+小鼠那样产生apoSAA反应,并且富含apoA1的HDL颗粒对于apoSAA在血浆中的运输并不是必需的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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