Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression最新文献_第10页

Molecular characterization of a bHLH transcription factor involved in Arabidopsis abscisic acid-mediated response 参与拟南芥脱落酸介导反应的bHLH转录因子的分子特征

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-03-01 DOI: 10.1016/j.bbaexp.2006.03.002

Jiyoung Kim, Ho-Young Kim

引用次数: 30

Retinoic acids and trichostatin A (TSA), a histone deacetylase inhibitor, induce human pyruvate dehydrogenase kinase 4 (PDK4) gene expression 视黄酸和组蛋白去乙酰化酶抑制剂曲古霉素A (TSA)诱导人丙酮酸脱氢酶激酶4 (PDK4)基因表达

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-03-01 DOI: 10.1016/j.bbaexp.2006.04.005

Hye-Sook Kwon, Boli Huang, Nam Ho Jeoung, Pengfei Wu, Calvin N. Steussy, Robert A. Harris

{"title":"Retinoic acids and trichostatin A (TSA), a histone deacetylase inhibitor, induce human pyruvate dehydrogenase kinase 4 (PDK4) gene expression","authors":"Hye-Sook Kwon, Boli Huang, Nam Ho Jeoung, Pengfei Wu, Calvin N. Steussy, Robert A. Harris","doi":"10.1016/j.bbaexp.2006.04.005","DOIUrl":"10.1016/j.bbaexp.2006.04.005","url":null,"abstract":"<div>Induction of pyruvate dehydrogenase kinase 4 (PDK4) conserves glucose and substrates for gluconeogenesis and thereby helps regulate blood glucose levels during starvation. We report here that retinoic acids (RA) as well as Trichostatin A (TSA), an inhibitor of histone deacetylase (HDAC), regulate PDK4 gene expression. Two retinoic acid response elements (RAREs) to which retinoid X receptor α (RXRα) and retinoic acid receptor α (RARα) bind and activate transcription are present in the human PDK4 (hPDK4) proximal promoter. Sp1 and CCAAT box binding factor (CBF) bind to the region between two RAREs. Mutation of either the Sp1 or the CBF site significantly decreases basal expression, transactivation by RXRα/RARα/RA, and the ability of TSA to stimulate hPDK4 gene transcription. By the chromatin immunoprecipitation assay, RA and TSA increase acetylation of histones bound to the proximal promoter as well as occupancy of CBP and Sp1. Interaction of p300/CBP with E1A completely prevented hPDK4 gene activation by RXRα/RARα/RA and TSA. The p300/CBP may enhance acetylation of histones bound to the hPDK4 promoter and cooperate with Sp1 and CBF to stimulate transcription of the hPDK4 gene in response to RA and TSA.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1759 3","pages":"Pages 141-151"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2006.04.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26073369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Protein–DNA interactions in the promoter region of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide of the cotton bollworm, Helicoverpa armigera 棉铃虫滞育激素和信息素生物合成激活神经肽基因启动子区蛋白与dna的相互作用

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-03-01 DOI: 10.1016/j.bbaexp.2006.03.003

Bo Hong , Zhi-Fang Zhang , Shun-Ming Tang , Yong-Zhu Yi , Tian-Yi Zhang , Wei-Hua Xu

{"title":"Protein–DNA interactions in the promoter region of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide of the cotton bollworm, Helicoverpa armigera","authors":"Bo Hong , Zhi-Fang Zhang , Shun-Ming Tang , Yong-Zhu Yi , Tian-Yi Zhang , Wei-Hua Xu","doi":"10.1016/j.bbaexp.2006.03.003","DOIUrl":"10.1016/j.bbaexp.2006.03.003","url":null,"abstract":"<div>Diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN) are two crucial neuropeptides which regulate insect development and sex pheromone biosynthesis respectively. These peptides are encoded by a single gene, termed DH-PBAN gene. In this study, we characterized the promoter of the DH-PBAN gene in Helicoverpa armigera (Har). Transient transfection assays using a series of stepwise deletion fragments linked to the luciferase reporter gene indicate that the promoter contains multiple regulator domains that can activate and repress reporter gene expression. The fragment spanning −467 to −371 bp of the DH-PBAN promoter is an activator domain of transcription, whereas the region from −965 to −534 bp represses the promoter activity in the insect cell line BmN. Electrophoretic mobility shift assays demonstrate that at least two nuclear protein factors from the nuclear protein extracts of H. armigera suboesophageal ganglion, Har-DHMBP-1 and-2 (DH-modulator-binding protein) can specifically bind to the activating region. Furthermore, we characterized in detail that the nuclear protein factor Har-DHMBP-3 can specifically bind to a classical E-box, CAGCTG localized at positions −360 to −355 bp, a potential site for interaction with basic helix–loop–helix transcription factors. Mutation of this E-box results in a significant reduction of the promoter activity, suggesting it can modulate the previously identified activator domain. Taken together, multipartite cis-elements and transcription factors in the DH-PBAN promoter are involved in regulation of the gene expression.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1759 3","pages":"Pages 177-185"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2006.03.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26397240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

DNA topoisomerase I from parasitic protozoa: A potential target for chemotherapy 寄生原生动物的DNA拓扑异构酶I:化疗的潜在靶点

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-03-01 DOI: 10.1016/j.bbaexp.2006.03.006

R.M. Reguera, C.M. Redondo, R. Gutierrez de Prado, Y. Pérez-Pertejo, R. Balaña-Fouce

{"title":"DNA topoisomerase I from parasitic protozoa: A potential target for chemotherapy","authors":"R.M. Reguera, C.M. Redondo, R. Gutierrez de Prado, Y. Pérez-Pertejo, R. Balaña-Fouce","doi":"10.1016/j.bbaexp.2006.03.006","DOIUrl":"10.1016/j.bbaexp.2006.03.006","url":null,"abstract":"<div>The growing occurrence of drug resistant strains of unicellular prokaryotic parasites, along with insecticide-resistant vectors, are the factors contributing to the increased prevalence of tropical diseases in underdeveloped and developing countries, where they are endemic. Malaria, cryptosporidiosis, African and American trypanosomiasis and leishmaniasis threaten human beings, both for the high mortality rates involved and the economic loss resulting from morbidity. Due to the fact that effective immunoprophylaxis is not available at present; preventive sanitary measures and pharmacological approaches are the only sources to control the undesirable effects of such diseases. Current anti-parasitic chemotherapy is expensive, has undesirable side effects or, in many patients, is only marginally effective. Under this point of view molecular biology techniques and drug discovery must walk together in order to find new targets for chemotherapy intervention. The identification of DNA topoisomerases as a promising drug target is based on the clinical success of camptothecin derivatives as anticancer agents. The recent detection of substantial differences between trypanosome and leishmania DNA topoisomerase IB with respect to their homologues in mammals has provided a new lead in the study of the structural determinants that can be effectively targeted. The present report is an up to date review of the new findings on type IB DNA topoisomerase in unicellular parasites and the role of these enzymes as targets for therapeutic agents.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1759 3","pages":"Pages 117-131"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2006.03.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26075005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

A novel member of the NSF family in the corn earworm, Helicoverpa zea: molecular cloning, developmental expression, and tissue distribution 玉米穗虫NSF家族新成员:分子克隆、发育表达和组织分布

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-03-01 DOI: 10.1016/j.bbaexp.2006.04.001

Wei-Hua Xu , Qi-Rui Zhang , David L. Denlinger

引用次数: 2

Combinatorial regulation modules on GmSBP2 promoter: A distal cis-regulatory domain confines the SBP2 promoter activity to the vascular tissue in vegetative organs GmSBP2启动子的组合调控模块:一个远端顺式调控域将SBP2启动子的活性限制在营养器官的维管组织中

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-01-01 DOI: 10.1016/j.bbaexp.2006.02.002

Alessandro J. Waclawovsky , Rejane L. Freitas , Carolina S. Rocha , Luis Antônio S. Contim , Elizabeth P.B. Fontes

{"title":"Combinatorial regulation modules on GmSBP2 promoter: A distal cis-regulatory domain confines the SBP2 promoter activity to the vascular tissue in vegetative organs","authors":"Alessandro J. Waclawovsky , Rejane L. Freitas , Carolina S. Rocha , Luis Antônio S. Contim , Elizabeth P.B. Fontes","doi":"10.1016/j.bbaexp.2006.02.002","DOIUrl":"10.1016/j.bbaexp.2006.02.002","url":null,"abstract":"<div>The Glycine max sucrose binding protein (GmSBP2) promoter directs phloem-specific expression of reporter genes in transgenic tobacco. Here, we identified cis-regulatory domains (CRD) that contribute with positive and negative regulation for the tissue-specific pattern of the GmSPB2 promoter. Negative regulatory elements in the distal CRD-A (−2000 to −700) sequences suppressed expression from the GmSBP2 promoter in tissues other than seed tissues and vascular tissues of vegetative organs. Deletion of this region relieved repression resulting in a constitutive promoter highly active in all tissues analyzed. Further deletions from the strong constitutive −700GmSBP2 promoter delimited several intercalating enhancer-like and repressing domains that function in a context-dependent manner. Histochemical examination revealed that the CRD-C (−445 to −367) harbors both negative and positive elements. This region abolished promoter expression in roots and in all tissues of stems except for the inner phloem. In contrast, it restores root meristem expression when fused to the −132pSBP2-GUS construct, which contains root meristem expression-repressing determinants mapped to the 44-bp CRD-G (−136 to −92). Thus, the GmSBP2 promoter is functionally organized into a proximal region with the combinatorial modular configuration of plant promoters and a distal domain, which restricts gene expression to the vascular tissues in vegetative organs.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1759 1","pages":"Pages 89-98"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2006.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25939685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

dps expression in Escherichia coli O157:H7 requires an extended − 10 region and is affected by the cAMP receptor protein dps在大肠杆菌O157:H7中的表达需要一个扩展的−10区，并受cAMP受体蛋白的影响

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-01-01 DOI: 10.1016/j.bbaexp.2006.02.001

Kwang-Cheol Jeong , David J. Baumler , Charles W. Kaspar

{"title":"dps expression in Escherichia coli O157:H7 requires an extended − 10 region and is affected by the cAMP receptor protein","authors":"Kwang-Cheol Jeong , David J. Baumler , Charles W. Kaspar","doi":"10.1016/j.bbaexp.2006.02.001","DOIUrl":"10.1016/j.bbaexp.2006.02.001","url":null,"abstract":"<div>The DNA binding protein from starved cells (Dps) is a general stress protein that provides Escherichia coli protection from osmotic, oxidative, and acid stresses. While Dps production and accumulation is primarily associated with stationary phase, during log phase, this protein protects against oxidative stress in an OxyR-dependent manner. In this study, evidence is provided that expands the role of Dps in acid tolerance to both log- and stationary-phase E. coli O157:H7. The transcription of dps occurred in log-phase cells without OxyR or stress and was upregulated during entry into stationary phase. The expression in log and stationary phase involved σ70 and σs, respectively, with both sigma factors recognizing the same promoter region. Site-directed mutagenesis identified an extended −10 region that was essential to both σ70 and σs transcription of dps. cAMP receptor protein (CRP) was found to repress dps expression as a crp mutant had a significant increase in the dps mRNA level. However, a CRP binding site was not found in the dps promoter and upregulation of dps in the crp mutant was absent in a crp rpoS double mutant. The findings from this study demonstrated that dps was expressed at a basal level during growth, both σ70- and σs-driven transcription required an extended −10, and CRP repression is mediated through the alternative sigma factor σs (rpoS).</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1759 1","pages":"Pages 51-59"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2006.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25939687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Identification and regulation of novel PPAR-γ splice variants in human THP-1 macrophages 人THP-1巨噬细胞中新型PPAR-γ剪接变异的鉴定和调控

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-01-01 DOI: 10.1016/j.bbaexp.2006.01.005

Ye Chen, Anna R. Jimenez, Jheem D. Medh

{"title":"Identification and regulation of novel PPAR-γ splice variants in human THP-1 macrophages","authors":"Ye Chen, Anna R. Jimenez, Jheem D. Medh","doi":"10.1016/j.bbaexp.2006.01.005","DOIUrl":"https://doi.org/10.1016/j.bbaexp.2006.01.005","url":null,"abstract":"<div>We have previously identified four novel isoforms of PPAR-γ transcripts in monkey macrophages (J. Zhou, K.M. Wilson, J.D. Medh, Genetic analysis of four novel peroxisome proliferator receptor-γ splice variants in monkey macrophages. Biochem. Biophys. Res. Commun., 293 (2002) 274-283). The purpose of this study was to ascertain that these isoforms are also present in humans. Specific primers were designed to amplify individual isoform transcripts. The presence of PPAR-γ4, PPAR-γ5, and PPAR-γ7 transcripts in human THP-1 macrophages was confirmed by RT-PCR and sequencing. A transcript corresponding to PPAR-γ6 was not detected. The presence of novel full-length transcripts and protein was also ascertained by Northern and Western blot analysis. Treatment of THP-1 cells with 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) resulted in more than 20% induction in the expression of PPAR-γ5 and PPAR-γ7 transcripts by both Northern blot analysis and RT-PCR. Another PPAR-γ ligand, troglitazone, induced expression of only PPAR-γ5. Both ligands inhibited the expression of PPAR-γ1 and PPAR-γ2. Additionally, 15d-PGJ2 and troglitazone increased the level of apolipoprotein E transcript by 60% but decreased lipoprotein lipase expression by 15% in THP-1 cells. The differential regulation of PPAR-γ transcripts suggests that each transcript isoform may contribute to macrophage function.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1759 1","pages":"Pages 32-43"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2006.01.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91758434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Characterization of human Enah gene 人Enah基因的表征

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-01-01 DOI: 10.1016/j.bbaexp.2006.01.001

Lorena Urbanelli , Carlo Massini , Carla Emiliani , Antonio Orlacchio , Giorgio Bernardi , Aldo Orlacchio

{"title":"Characterization of human Enah gene","authors":"Lorena Urbanelli , Carlo Massini , Carla Emiliani , Antonio Orlacchio , Giorgio Bernardi , Aldo Orlacchio","doi":"10.1016/j.bbaexp.2006.01.001","DOIUrl":"10.1016/j.bbaexp.2006.01.001","url":null,"abstract":"<div>Enabled homolog (Enah) is a mammalian ortholog of Drosophila Enabled (Ena), which is genetically linked to the Drosophila Abl tyrosine phosphorylation signaling cascade and is required for normal neural development. Vertebrates have three Ena-related genes: Enah, VASP (vasodilator-stimulated phosphoprotein) and Ena/VASP like (EVL). These genes play an important role in linking signal transduction pathways to localized remodeling of the actin cytoskeleton. We isolated and sequenced a cDNA encoding human Enah. Comparison of the amino acid sequences of mouse (Mus musculus) and human (Homo sapiens) species shows 86.6% identity. The human protein appears longer than the mouse and additional amino acids are concentrated in a region containing repeats of the amino acid sequence LERER. The complete gene is about 157 kb and consists of 14 exons. Analysis of multiple tissue northern blot revealed a major transcript of about 4.8 kb in all tissue examined. Alternatively spliced isoforms were isolated by RT-PCR. The gene is differentially expressed and to gain insight factors affecting its expression we cloned and preliminarily characterized human Enah gene promoter.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1759 1","pages":"Pages 99-107"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2006.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25869657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Physical and functional interactions of Arabidopsis ADA2 transcriptional coactivator proteins with the acetyltransferase GCN5 and with the cold-induced transcription factor CBF1 拟南芥ADA2转录辅激活蛋白与乙酰转移酶GCN5和冷诱导转录因子CBF1的物理和功能相互作用

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-01-01 DOI: 10.1016/j.bbaexp.2006.02.006

Yaopan Mao , Kanchan A. Pavangadkar , Michael F. Thomashow , Steven J. Triezenberg

{"title":"Physical and functional interactions of Arabidopsis ADA2 transcriptional coactivator proteins with the acetyltransferase GCN5 and with the cold-induced transcription factor CBF1","authors":"Yaopan Mao , Kanchan A. Pavangadkar , Michael F. Thomashow , Steven J. Triezenberg","doi":"10.1016/j.bbaexp.2006.02.006","DOIUrl":"10.1016/j.bbaexp.2006.02.006","url":null,"abstract":"<div>The Arabidopsis GCN5, ADA2a and ADA2b proteins are homologs of components of several yeast and animal transcriptional coactivator complexes. Previous work has implicated these plant coactivator proteins in the stimulation of cold-regulated gene expression by the transcriptional activator protein CBF1. Surprisingly, protein interaction studies demonstrate that the DNA-binding domain of CBF1 (and of a related protein, TINY), rather than its transcriptional activation domain, can bind directly to the Arabidopsis ADA2 proteins. The ADA2a and ADA2b proteins can also bind directly to GCN5 through their N-terminal regions (comparable to a region previously defined in yeast Ada2) and through previously unmapped regions in the middle of the ADA2 proteins, which bind to the HAT domain of GCN5. The ADA2 proteins enhance the ability of GCN5 to acetylate histones in vitro and enable GCN5 to acetylate nucleosomal histones. Moreover, GCN5 can acetylate the ADA2 proteins at a motif unique to the plant homologs and absent from fungal and animal homologs. We speculate that this modification may represent a novel autoregulatory mechanism for the plant SAGA-like transcriptional coactivator complexes.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1759 1","pages":"Pages 69-79"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2006.02.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25963678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 97