Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression最新文献

Promoter analysis of the rice stemar-13-ene synthase gene OsDTC2, which is involved in the biosynthesis of the phytoalexin oryzalexin S 水稻茎秆13烯合成酶基因OsDTC2的启动子分析，该基因参与植物抗菌素oryzalexin S的生物合成

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/j.bbaexp.2007.08.007

Tadahiro Nemoto , Atsushi Okada , Kazunori Okada , Naoto Shibuya , Tomonobu Toyomasu , Hideaki Nojiri , Hisakazu Yamane

{"title":"Promoter analysis of the rice stemar-13-ene synthase gene OsDTC2, which is involved in the biosynthesis of the phytoalexin oryzalexin S","authors":"Tadahiro Nemoto , Atsushi Okada , Kazunori Okada , Naoto Shibuya , Tomonobu Toyomasu , Hideaki Nojiri , Hisakazu Yamane","doi":"10.1016/j.bbaexp.2007.08.007","DOIUrl":"10.1016/j.bbaexp.2007.08.007","url":null,"abstract":"<div>A rice diterpene cyclase, OsDTC2, functions as a stemar-13-ene synthase that converts syn-copalyl diphosphate into stemar-13-ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S. The transcriptional expression of OsDTC2 is induced by treatment of suspension-cultured rice cells with a chitin oligosaccharide elicitor. To investigate the molecular mechanisms of the elicitor signaling pathway that leads to OsDTC2 expression, we carried out deletion and mutation analysis of the region −1939 bp upstream of the transcription start site of OsDTC2 in rice cells using dual luciferase assays. The region between −1709 and −1450 bp was found to contain six W-box motifs, which are putative recognition sites for WRKY transcription factors, as cis elements involved in elicitor-responsiveness and/or basic promoter activity of OsDTC2.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 11","pages":"Pages 678-683"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.08.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27069110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Basic helix–loop–helix factors recruit nuclear factor I to enhance expression of the NaV 1.4 Na+ channel gene 碱性螺旋-环-螺旋因子招募核因子I，增强NaV 1.4 Na+通道基因的表达

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/j.bbaexp.2007.08.004

Sadie L. Hebert , Christine Simmons , Amy L. Thompson , Catherine S. Zorc , Eric M. Blalock , Susan D. Kraner

{"title":"Basic helix–loop–helix factors recruit nuclear factor I to enhance expression of the NaV 1.4 Na+ channel gene","authors":"Sadie L. Hebert , Christine Simmons , Amy L. Thompson , Catherine S. Zorc , Eric M. Blalock , Susan D. Kraner","doi":"10.1016/j.bbaexp.2007.08.004","DOIUrl":"10.1016/j.bbaexp.2007.08.004","url":null,"abstract":"<div>We have previously shown that the basic helix–loop–helix (bHLH) transcription factors coordinate NaV 1.4 Na+ channel gene expression in skeletal muscle, but the identity of the co-factors they direct is unknown. Using C2C12 muscle cells as a model system, we test the hypothesis that the bHLH factors counteract negative regulation exerted through a repressor E box (−90/−85) by recruiting positive-acting transcription factors to the nucleotides (−135/−57) surrounding the repressor E box. We used electrophoretic mobility shift assays to identify candidate factors that bound the repressor E box or these adjacent regions. Repressor E box-binding factors included the known transcription factor, ZEB/AREB6, and a novel repressor E box-binding factor designated REB. Mutations of the repressor E box that interfere with the binding of these factors prevented repression. The transcription factor, nuclear factor I (NFI), bound immediately upstream and downstream of the repressor E box. Mutation of the NFI-binding sites diminished the ability of myogenin and MRF4 to counteract repression. Based on these observations we suggest that bHLH factors recruit NFI to enhance skeletal muscle Na+ channel expression.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 11","pages":"Pages 649-658"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.08.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27049005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Cumulative Contents 累积的内容

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/S0167-4781(07)00167-4

引用次数: 0

TF2 binds to the regulatory promoter of alkaline phosphatase in Dicytostelium TF2结合双歧菌碱性磷酸酶的调控启动子

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/j.bbaexp.2007.07.006

Bradley R. Joyce, Natasha S. Wiles, Charles L. Rutherford

{"title":"TF2 binds to the regulatory promoter of alkaline phosphatase in Dicytostelium","authors":"Bradley R. Joyce, Natasha S. Wiles, Charles L. Rutherford","doi":"10.1016/j.bbaexp.2007.07.006","DOIUrl":"10.1016/j.bbaexp.2007.07.006","url":null,"abstract":"<div>Alkaline phosphatase (ALP) activity becomes restricted to PstO cells at the prestalk–prespore boundary during the later stages of development, suggesting a novel function in the regulation of prestalk cell differentiation. To identify regulatory control sequences within the alp promoter, a series of 5′ and internal deletions were generated and fused to the LacZ reporter gene. In vitro assays of reporter activity from Dicytostelium transformants containing the deleted promoter–LacZ fusion constructs showed that the −683 to −468 bp sequence is required for proper activation of the reporter in developing slugs. To identify DNA–protein interactions involved in the regulation of alp, EMSAs were preformed using a series of short overlapping PCR probes that span the regulatory promoter sequence. A sequence-specific DNA-binding protein was identified that interacts with the −665 to −635 bp sequence. This DNA-binding protein was sequentially purified using DEAE-Sephacel, heparin-Sepharose, DNA Affinity, and gel filtration chromatography. A polypeptide with a molecular weight of 28 kDa was identified on an SDS-PAGE. The purified protein was identified as TF2 by mass spectrometry. TF2 may, therefore, bind to the regulatory promoter of alp and function in the developmental control of PstO differentiation in Dicytostelium.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 11","pages":"Pages 668-677"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.07.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27031044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of gene expression in human cells using RNase P-derived ribozymes and external guide sequences 利用RNase - p衍生核酶和外部引导序列抑制人细胞中的基因表达

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/j.bbaexp.2007.09.001

Kihoon Kim , Fenyong Liu

{"title":"Inhibition of gene expression in human cells using RNase P-derived ribozymes and external guide sequences","authors":"Kihoon Kim , Fenyong Liu","doi":"10.1016/j.bbaexp.2007.09.001","DOIUrl":"10.1016/j.bbaexp.2007.09.001","url":null,"abstract":"<div>Ribonuclease P (RNase P) complexed with an external guide sequence (EGS) represents a novel nucleic acid-based gene interference approach to modulate gene expression. This enzyme is a ribonucleoprotein complex for tRNA processing. In Escherichia coli, RNase P contains a catalytic RNA subunit (M1 ribozyme) and a protein subunit (C5 cofactor). EGSs, which are RNAs derived from natural tRNAs, bind to a target mRNA and render the mRNA susceptible to hydrolysis by RNase P and M1 ribozyme. When covalently linked with a guide sequence, M1 can be engineered into a sequence-specific endonuclease, M1GS ribozyme, which cleaves any target RNAs that base pair with the guide sequence. Studies have demonstrated efficient cleavage of mRNAs by M1GS and RNase P complexed with EGSs in vitro. Moreover, highly active M1GS and EGSs were successfully engineered using in vitro selection procedures. EGSs and M1GS ribozymes are effective in blocking gene expression in both bacteria and human cells, and exhibit promising activity for antimicrobial, antiviral, and anticancer applications. In this review, we highlight some recent results using the RNase P-based technology, and offer new insights into the future of using EGS and M1GS RNA as tools for basic research and as gene-targeting agents for clinical applications.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 11","pages":"Pages 603-612"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41010091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

A novel promoter regulates calcitonin receptor gene expression in human osteoclasts 一种新的启动子调控人类破骨细胞降钙素受体基因表达

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/j.bbaexp.2007.08.005

Zhenxin Shen , Tania N. Crotti , Merrilee R. Flannery , Kenichiro Matsuzaki , Steven R. Goldring , Kevin P. McHugh

{"title":"A novel promoter regulates calcitonin receptor gene expression in human osteoclasts","authors":"Zhenxin Shen , Tania N. Crotti , Merrilee R. Flannery , Kenichiro Matsuzaki , Steven R. Goldring , Kevin P. McHugh","doi":"10.1016/j.bbaexp.2007.08.005","DOIUrl":"10.1016/j.bbaexp.2007.08.005","url":null,"abstract":"<div>The calcitonin receptor (CTR) is expressed in a wide variety of tissues and cell types. In bone, its expression is restricted to osteoclasts, the cells that mediate bone resorption. The human CTR (hCTR) gene has a complex structural organization that exhibits similarity to the porcine (pCTR) and mouse (mCTR) CTR genes. In these species, alternative splicing of a single gene generates multiple CTR isoforms that are distributed in both tissue-specific and species-specific patterns. However, the structural organization of the 5′ putative regulatory region and transcriptional mechanisms responsible for tissue-specific expression of the different CTR isoforms are not fully defined. The present studies were undertaken to characterize the structural organization of the 5′-region of the hCTR and identify the regulatory regions involved in osteoclast-specific transcriptional activation. Analysis of mRNA prepared from human osteoclasts using reverse transcription-polymerase chain reaction (RT-PCR) and transient transfection of hCTR promoter-luciferase reporter constructs identified two regions in the 5′-flanking sequence of the hCTR gene that regulated CTR gene expression in osteoclasts. Both of these putative promoters were responsive to the osteoclast-inducing cytokine, receptor activator of NF-κB ligand (RANKL) and demonstrated trans-activation by the RANKL-induced transcription factor nuclear factor of activated T cells (NFATc1), consistent with a role in regulating CTR gene expression in osteoclasts.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 11","pages":"Pages 659-667"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.08.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27060169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

IGF-1 controls GLUT3 expression in muscle via the transcriptional factor Sp1 IGF-1通过转录因子Sp1控制GLUT3在肌肉中的表达

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/j.bbaexp.2007.08.002

John A. Copland , Aaron W. Pardini , Thomas G. Wood , Deling Yin , Allan Green , Yvonne H. Bodenburg , Randall J. Urban , Charles A. Stuart

{"title":"IGF-1 controls GLUT3 expression in muscle via the transcriptional factor Sp1","authors":"John A. Copland , Aaron W. Pardini , Thomas G. Wood , Deling Yin , Allan Green , Yvonne H. Bodenburg , Randall J. Urban , Charles A. Stuart","doi":"10.1016/j.bbaexp.2007.08.002","DOIUrl":"10.1016/j.bbaexp.2007.08.002","url":null,"abstract":"<div>Glucose transporter 3 (GLUT3), while first found in human fetal muscle, is predominantly expressed in brain and neural tissue. By several independent techniques we have previously shown that GLUT3 is expressed in human skeletal muscle cells. The structure of the human GLUT3 gene has not been previously reported nor has there been any evaluation of the 5′-untranslated region (UTR). To this end, we have cloned and sequenced the human GLUT3 gene. Insulin-like growth factor-1 (IGF-1) increased endogenous Glut3 protein in cultured L6 myotubes, and similarly stimulated luciferase activity in a construct of the human GLUT3 5′-UTR linked to a luciferase reporter gene. Actinomycin D, an inhibitor of mRNA synthesis, prevented IGF-1 stimulation of Glut3 protein. Transfection of L6 cells with Sp1 increased Glut3 and augmented IGF-1 stimulation of Glut3 expression. Knockdown of Glut3 expression in cultured L6 muscle cells using small interference RNA (siRNA) specific for Glut3 significantly reduced myocyte glucose uptake. DNAse footprinting and gel shift assays showed Sp1 specifically bound to the human GLUT3 5′-UTR. Substitution mutants of the human GLUT3 5′-UTR luciferase construct indicated that only one of three Sp1 site clusters was involved in IGF-1 action. These data, using both a human GLUT3 5′-UTR construct and L6 cells' endogenous promoter, suggest that IGF-1 plays a role in maintaining muscle GLUT3 expression and basal glucose uptake via the transcriptional factor Sp1.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 11","pages":"Pages 631-640"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.08.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27034095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Signal-induced functions of the transcription factor TFII-I 转录因子TFII-I -i的信号诱导功能

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/j.bbaexp.2007.10.002

Ananda L. Roy

{"title":"Signal-induced functions of the transcription factor TFII-I","authors":"Ananda L. Roy","doi":"10.1016/j.bbaexp.2007.10.002","DOIUrl":"10.1016/j.bbaexp.2007.10.002","url":null,"abstract":"<div>We have learned a great deal over the last several years about the molecular mechanisms that govern cell growth, cell division and cell death. Normal cells pass through cell cycle (growth) and divide in response to mitogenic signals that are transduced through their cognate cell surface receptors to the nucleus. Despite the fact that cellular growth and division are mechanistically distinct steps, they are usually coordinately regulated, which is critical for normal cellular proliferation. The precise mechanistic basis for this coordinated regulation is unclear. TFII-I is a unique, signal-induced multifunctional transcription factor that is activated upon a variety of signaling pathways and appears to participate in distinct phases of cell growth. For instance, TFII-I is required for growth factor-induced transcriptional activation of the c-fos gene, which is essential for cell cycle entry. Two alternatively spliced isoforms of TFII-I exhibit opposing but necessary functions for mitogen-induced transcriptional activation of c-fos. Besides transcriptional activation of the c-fos proto-oncogene and eventual entry into cell cycle, TFII-I also appears to have a role in later phases of the cell cycle and cell division. Here we discuss how a multitude of signaling inputs target TFII-I isoforms, which may exert their functions in distinct phases of the cell cycle and play a key role in the coordinated regulation of cellular proliferation.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 11","pages":"Pages 613-621"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.10.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41077681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

Expression of the human CMP-NeuAc:GM3 α2,8-sialyltransferase (GD3 synthase) gene through the NF-κB activation in human melanoma SK-MEL-2 cells 人CMP-NeuAc:GM3 α2,8-唾液基转移酶(GD3合成酶)基因通过NF-κB活化在人黑色素瘤SK-MEL-2细胞中的表达

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/j.bbaexp.2007.08.001

Nam-Young Kang , Cheorl-Ho Kim , Kyoung-Sook Kim , Jeong-Heon Ko , Jai-Heon Lee , Yong-Kee Jeong , Young-Choon Lee

{"title":"Expression of the human CMP-NeuAc:GM3 α2,8-sialyltransferase (GD3 synthase) gene through the NF-κB activation in human melanoma SK-MEL-2 cells","authors":"Nam-Young Kang , Cheorl-Ho Kim , Kyoung-Sook Kim , Jeong-Heon Ko , Jai-Heon Lee , Yong-Kee Jeong , Young-Choon Lee","doi":"10.1016/j.bbaexp.2007.08.001","DOIUrl":"10.1016/j.bbaexp.2007.08.001","url":null,"abstract":"<div>To elucidate the mechanism underlying the regulation of human GD3 synthase gene expression in human melanoma SK-MEL-2 cells, we identified the promoter region of the human GD3 synthase gene. The 5′-rapid amplification of cDNA end (5′-RACE) using mRNA prepared from SK-MEL-2 cells revealed the presence of multiple transcription start sites of human GD3 synthase gene. Promoter analyses of the 5′-flanking region of the human GD3 synthase gene using luciferase gene reporter system showed the strong promoter activity in SK-MEL-2 cells. Deletion study revealed that the region as the core promoter from −1146 to −646 (A of the translational start ATG as position +1) was indispensable for endogenous expression of human GD3 synthase gene. This region lacks apparent TATA and CAAT boxes but contains putative binding sites for transcription factors c-Ets-1, CREB, AP-1 and NF-κB. Electrophoretic mobility shift assays using specific competitors, chromatin immunoprecipitation assay and site-directed mutagenesis demonstrated that only NF-κB element in this region is required for the promoter activity in SK-MEL-2 cells. These results indicate that NF-κB plays an essential role in the transcriptional activity of human GD3 synthase gene essential for GD3 synthesis in SK-MEL-2 cells.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 11","pages":"Pages 622-630"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27028121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

CCAAT/Enhancer-binding protein β regulates expression of human T1R3 taste receptor gene in the bile duct carcinoma cell line, HuCCT1 CCAAT/增强子结合蛋白β调控人T1R3味觉受体基因在胆管癌细胞系HuCCT1中的表达

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-11-01 DOI: 10.1016/j.bbaexp.2007.08.003

Takashi Toyono, Yuji Seta, Shinji Kataoka, Kuniaki Toyoshima

{"title":"CCAAT/Enhancer-binding protein β regulates expression of human T1R3 taste receptor gene in the bile duct carcinoma cell line, HuCCT1","authors":"Takashi Toyono, Yuji Seta, Shinji Kataoka, Kuniaki Toyoshima","doi":"10.1016/j.bbaexp.2007.08.003","DOIUrl":"10.1016/j.bbaexp.2007.08.003","url":null,"abstract":"<div>The T1R family (T1R1, T1R2 and T1R3 receptors) has a role in the detection of umami and sweet tastes in taste buds. Although T1R3 is also expressed in the intrahepatic bile duct, the expression patterns of T1R1 and T1R2 in this region have not been determined. In addition, the mechanisms of transcriptional regulation of the human T1R3 gene (Tas1r3) have not been elucidated. In this study, we determined the expression patterns of T1R2 and T1R3 in human liver and the function of C/EBPβ in Tas1r3 promoter activity. Immunohistochemistry showed that T1R2 and T1R3 were expressed in the intrahepatic bile duct. 5′-RACE analysis revealed that the transcriptional start sites of Tas1r3 were located 67 bp and 176 bp upstream of the ATG. Promoter analysis of Tas1r3 was performed using the luciferase reporter assay and EMSA in the Tas1r3-expressing cell line, HuCCT1. The 226-bp region upstream of the ATG had promoter activity, and C/EBPβ activated the Tas1r3 promoter activity in HuCCT1 cells. These results show that T1R2 and T1R3 receptors, in addition to their role in taste perception, may also have a role in intrahepatic cholangiocytes. C/EBPβ was identified as the transcription factor regulating Tas1r3 promoter activity in HuCCT1 cells.</div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 11","pages":"Pages 641-648"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.08.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18