Chinese Journal of Integrative Medicine最新文献

{"title":"Application of Chinese Medicine in Treatment of Ulcerative Colitis and Elucidation of Relevant Mechanisms.","authors":"Ji-Wei Zhu, Han-Ming Wang, Muaitaer Aisikaer, Wen-Jun Zhou, Tong-Tong Yang, Kasimujiang Aximujiang","doi":"10.1007/s11655-025-3824-y","DOIUrl":"10.1007/s11655-025-3824-y","url":null,"abstract":"","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":"757-768"},"PeriodicalIF":3.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loganin Alleviates CCl₄-Induced Acute Liver Injury by Promoting Mitophagy and Inhibiting NLRP3 Inflammasome.","authors":"Meng Jiang, Xiao-Jia Yang, Li-Quan Liu, Yi-Li Zhang, Ping Huang, Min Wu, Yuan Deng","doi":"10.1007/s11655-026-3851-3","DOIUrl":"10.1007/s11655-026-3851-3","url":null,"abstract":"<p><strong>Objective: </strong>To explore the protective effect and mechanism of loganin, a iridoid glycoside isolated from Corni Fructus, on carbon tetrachloride (CCl₄)-induced acute liver injury (ALI) mice model and L-02 cells.</p><p><strong>Methods: </strong>ALI mice model was developed by an intraperitioneal injection of 0.3% CCl₄. Thirty 8-week-old male C57BL/6 mice were randomly divided into 5 groups using a random number table, including control, model, loganin (40 and 80 mg/kg), and silybin (100 mg/kg) groups (n=6). After 6 consecutive days of intragastric administration, serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured using an automatic biochemical analyzer. Hepatic pathological changes were observed by hematoxylineosin (HE) staining. Levels of interleukin (IL)-6 and tumor necrosis factor-α (TNF-α) in liver tissue were detected by enzyme-linked immunosorbent assay (ELISA). Superoxide dismutase (SOD) activity and malondialdehyde (MDA) contents were measured using biochemical kits. Mitochondrial ultrastructure and autophagosome formation were observed by transmission electron microscopy (TEM). Co-localization of mitophagy markers LC3 and TOMM20 were assessed by immunofluorescence (IF). Protein expressions of NOD-like receptor protein 3 (NLRP)3, LC3 II/LC3 I, p62, Beclin-1, Atg-7, Parkin, and PINK1 were determined by Western blot. In vitro, an injury model was established in L-02 hepatocytes stimulated with CCl4. Cell viability was assessed by CCK-8 assay, and related mechanisms were evaluated using the aforementioned methods. Besides, CCl₄-stimulated L-02 cells were intervened with NLRP3 shRNA or Mdivi-1.</p><p><strong>Results: </strong>Compared with the model group, pre-treatment with loganin (40 and 80 mg/kg) significantly reduced serum AST and ALT levels (P<0.01), alleviated pathological injuries such as swelling, necrosis, inflammatory infiltration, and lipid vacuolation in liver tissue. Loganin also markedly decreased the levels of IL-6 and TNF-α, increased SOD activity, and reduced MDA content in liver tissues (P<0.05 or P<0.01). Mechanistically, loganin up-regulated the ratios of LC3 II/LC3 I and expressions of Beclin-1, Atg-7, Parkin, and PINK1, while down-regulated p62 and NLRP3 protein expressions (P<0.05). In vitro experiments further confirmed that loganin attenuated CCl₄-induced injury in L-02 cells by enhancing mitophagy and inhibiting NLRP3 inflammasome activation, which was reversed by the mitophagy inhibitor Mdivi-1.</p><p><strong>Conclusion: </strong>Loganin protected against CCl₄-induced ALI both in vivo and in vitro by suppressing the NLRP3 inflammasome and enhancing mitophagy.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":"693-701"},"PeriodicalIF":3.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Jiedu Yizhi Formula for Alzheimer's Disease: A Randomized Controlled Trial.","authors":"Yun-Qiang Li, Dong-Xue Chen, Xiao-Ting Zhu, Wei Li, Jia-Li Wu, Li-Mei Fang, Ming-Quan Li","doi":"10.1007/s11655-026-3944-z","DOIUrl":"https://doi.org/10.1007/s11655-026-3944-z","url":null,"abstract":"<p><strong>Objective: </strong>To observe the clinical effect and safety of Jiedu Yizhi Formula (JYF) in Alzheimer's disease (AD) patients.</p><p><strong>Methods: </strong>Eligible patients with mild to moderate AD and Chinese medicine (CM) syndrome of Pi (Spleen)-Shen (Kidney) deficiency with phlegm-turbidity obscuring orifices were collected from the Third Affiliated Hospital of Changchun University of Chinese Medicine between December 2022 and December 2023. Patients were randomly assigned to JYF and donepezil groups at a 1:1 ratio by using a random number table and received a total of 24 weeks of continuous treatment. Both groups were given AD cognitive behavior training and health education at the same time. The Mini Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), AD Assessment Scale-Cognitive (ADAS-cog), Activity of Daily Living (ADL), and Hamilton Depression (HAMD) scale scores were measured before treatment, 12 and 24 weeks after treatment. The cognitive domain scores and the total effective rates were also compared between groups. During the study period, any adverse reactions were recorded and compared between groups.</p><p><strong>Results: </strong>Totally 62 participants completed the study, with 31 in each group. The MMSE, MoCA, ADAS-cog, ADL, and HAMD scores significantly improved after 12 and 24 weeks of treatment in both groups (P<0.05 or P<0.01). Moreover, significant improvements in the ADL and HAMD scores were observed in the JYF group at 12 or 24 weeks than in the donepezil group (P<0.05 or P<0.01). Meanwhile, the cognitive domain scores of orientational and abstraction abilities and the total effective rate (64.52% vs. 45.16%) in the JYF group were significantly higher (P<0.05). No significant difference was found in the incidence of adverse reactions between groups (9.68% vs. 6.45%, P>0.05).</p><p><strong>Conclusion: </strong>JYF can improve the cognitive dysfunction of mild to moderate AD patients with CM syndrome of Pi-Shen deficiency with phlegm-turbidity obscuring orifices, and its safety is good. (Trial registration No. NCT06393413).</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148629669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qingda Granule and Its Individual Herbs Inhibit Hypertension-Induced Vascular Smooth Muscle Cells Proliferation via ERK and JNK Pathways.","authors":"Jin-Kong Wu, Ren-Feng Li, Mei-Zhu Wu, Guo-Sheng Lin, Wen-Qiang Zhang, Zhi Guo, You-Qin Chen, Da-Wei Lian, A-Ling Shen, Jun Peng","doi":"10.1007/s11655-026-4041-z","DOIUrl":"https://doi.org/10.1007/s11655-026-4041-z","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the specific role and underlying mechanisms of individual herbs in Qingda Granule (QDG) in attenuating hypertension and inhibiting proliferation of vascular smooth muscle cells (VSMCs).</p><p><strong>Methods: </strong>Spontaneously hypertensive rats (SHRs) and angiotensin II (Ang II)-stimulated VSMCs were treated with QDG or its individual herbs. SHRs were divided into groups using a completely randomized design: SHRs, valsartan (Val), QDG, Gastrodia elata Blume (Tianma, Gas), Uncaria rhynchophylla Miz. ex Havil. (Gouteng, Unc), Scutellaria baicalensis Georgi (Huangqin, Scu), and Nelumbo nucifera Gaertn (Lianzixin, Nel), 6 in each group. All treatments were administered via intragastric gavage once daily for 10 weeks. Vascular function and pathological changes were evaluated by ultrasonography and HE staining. The proliferation of VSMCs was assayed by cell counting kit-8, immunohistochemistry (IHC), and Western blot. RNA sequencing was performed to identify differentially expressed transcripts (DETs) in the abdominal aorta. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed to identify the key signaling pathways, and the expression of pathway-related proteins was subsequently verified by IHC and Western blot.</p><p><strong>Results: </strong>QDG and its individual herb treatments significantly attenuated the increase in pulse wave velocity and aortic wall thickness in SHRs. QDG inhibited the increased PCNA expression and cell viability in VSMCs. Unc, Scu, and Nel exhibited effects similar to QDG, while Gas did not show these effects. RNA sequencing analysis identified DETs in abdominal aortic tissues. KEGG analysis based on the DETs revealed that multiple common and unique signaling pathways were enriched. Among them, the MAPK pathway was enriched in the QDG, Unc, Scu, and Nel groups, but not in the Gas group, and the cAMP pathway was significantly enriched in all groups. IHC and Western blot analysis verified that compared with the SHR group, the QDG, Unc, Scu, and Nel groups inhibited MAPK pathway activation and promoted cAMP pathway activation in the abdominal aorta of SHR and Ang II-stimulated A7R5s (P<0.05). However, Gas only inhibited the activation of the p38 MAPK pathway and promoted the activation of the cAMP pathway (P<0.05).</p><p><strong>Conclusions: </strong>The herbs in QDG exert significant antihypertensive effects by regulating common and unique pathways. Specifically, Unc, Scu, and Nel inhibit ERK and JNK pathway activation, which likely underlies QDG's suppression of VSMCs proliferation.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148629648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Safety of Zizhu Ointment for Wagner Grade 2 Diabetic Foot Ulcers: A Single-Center, Randomized, Double-Blind, Placebo-Controlled Trial.","authors":"Xiao-Yu Li, Guo-Bin Liu, Yang You, Wei-Jing Fan","doi":"10.1007/s11655-026-4162-4","DOIUrl":"https://doi.org/10.1007/s11655-026-4162-4","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the clinical efficacy and safety of Zizhu Ointment (ZZO) in treating Wagner grade 2 diabetic foot ulcers (DFUs).</p><p><strong>Methods: </strong>In this single-center, randomized, double-blind, placebo-controlled trial, using SPSS 26.0, a computer-generated random number sequence was used to randomly assign 154 patients with Wagner grade 2 DFUs in a 1:1 ratio to either the ZZO group (n=77) or the placebo control group (n=77). Both groups received conventional therapy and standard debridement. Patients received the assigned intervention as a topical application for 6 weeks, followed by a 6-week follow-up period. The primary outcome was the ulcer healing rate at 6 weeks. Secondary outcomes included Chinese medicine (CM) syndrome scores, transcutaneous oxygen pressure (TcPO₂), wound blood flow, and safety indicators.</p><p><strong>Results: </strong>After 6 weeks, the ulcer healing rate was significantly higher in the ZZO group than in the placebo control group (67.59% ± 22.69% vs. 28.81% ± 15.80%, P<0.001). This superiority was maintained at the end of the 6-week follow-up (80.95% ±19.16% vs. 42.20% ±21.93%, P<0.001). The ZZO group also demonstrated significantly greater improvements in reducing ulcer area and depth, improving CM syndrome scores, TcPO₂, and wound blood flow (all P<0.05). The incidence of adverse events was comparable between the two groups (P>0.05).</p><p><strong>Conclusions: </strong>ZZO effectively promotes the healing of Wagner grade 2 DFUs, ameliorates the wound microenvironment and CM symptoms, and exhibits a favorable safety profile. (Registration No. ITMCTR2025000126).</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148599785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chinese Medicine for Renal Fibrosis Prevention and Treatment in Chronic Kidney Disease: Pharmacological Mechanisms and Therapeutic Potential.","authors":"Qiao-Hua Wang, Xin Song, Nian-Hua Ye, Qiao-Qin Wang, Li-Sheng Jiang, Ming-Hua Ye","doi":"10.1007/s11655-026-4238-1","DOIUrl":"https://doi.org/10.1007/s11655-026-4238-1","url":null,"abstract":"","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148599759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Press Needle Combining Breast Massage on Onset of Lactogenesis II in Post-Cesarean Mothers: A Randomized Controlled Trial.","authors":"Tian Gao, Qiu-Ning Cao, Jia Qiao","doi":"10.1007/s11655-026-4245-2","DOIUrl":"https://doi.org/10.1007/s11655-026-4245-2","url":null,"abstract":"<p><strong>Objective: </strong>To assess the effectiveness of press needle acupoint stimulation and breast massage on lactation outcomes.</p><p><strong>Methods: </strong>This two-arm randomized controlled trial enrolled 132 first-time mothers undergoing cesarean section between December 2023 to June 2024. Participants were randomly assigned in a 1:1 ratio to an experimental group or a control group (66 cases in each) using a computer-generated block randomization sequence. Both groups received routine post-cesarean care, early skin-to-skin contact, and breastfeeding guidance. In addition, the experimental group received press needle acupoint stimulation initiated within 4-6 h after cesarean delivery and breast massage combined with low-frequency electronic pulse stimulation 3 times daily for 3 days. The primary outcome was the onset time of lactogenesis II. Secondary and mechanistic outcomes included breastfeeding frequency, formula supplementation, exclusive breastfeeding rate, infant weight change within 72 h, serum prolactin and oxytocin levels, and breast milk composition at 72 h postpartum. Safety was monitored by recording any maternal or neonatal adverse events during the intervention period.</p><p><strong>Results: </strong>The experimental group showed earlier lactogenesis II onset, higher breastfeeding frequency at 24-48 h and 48-72 h, and higher exclusive breastfeeding rates at 72 h (P<0.01, P=0.013, 0.034, respectively). Mothers in the experimental group used less formula at 72 h postpartum (P=0.022). In addition, breast milk from the experimental group contained higher levels of protein, fat, and minerals (P=0.021, 0.027, P<0.01, respectively), as well as a slightly higher water content [P=0.022]. Maternal serum prolactin and oxytocin levels were also significantly higher in the experimental group (both P<0.01). Infants in the experimental group also had less weight loss at 72 h (P<0.01). No serious adverse events were observed.</p><p><strong>Conclusion: </strong>Press needle acupoint stimulation and breast massage effectively enhance early lactation outcomes. Further studies are needed to evaluate their long-term impacts on breastfeeding duration and maternal health. (Registration No. NCT06498882).</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148599753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Medicine for Liver Fibrosis: Bioactive Constituents and Therapeutic Potential of Scutellaria baicalensis.","authors":"An-Zhao Wu, Lu-Yan Sun","doi":"10.1007/s11655-026-3852-2","DOIUrl":"https://doi.org/10.1007/s11655-026-3852-2","url":null,"abstract":"","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperoside Exerts Therapeutic Effects on Parkinson's Disease by Mitigating Oxidative Stress through Activation of Nrf2/HO-1 Pathway.","authors":"Si-Xiang Niu, Li-Shan Fan, Jing-Yi Cheng, Lei Xu, Li-Xia Yang, Sheng-Nan Xiao, Rui-Rui Sun, Shao-Chen Qin, Bao-Guo Xiao, Hui-Jie Fan, Zhi Chai","doi":"10.1007/s11655-026-4160-6","DOIUrl":"https://doi.org/10.1007/s11655-026-4160-6","url":null,"abstract":"<p><strong>Objective: </strong>To investigate whether hyperoside (HYP), the main active ingredients of Wuzi Yanzong Pill, can reduce oxidative stress (OS) damage and treat Parkinson's disease (PD) by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway.</p><p><strong>Methods: </strong>MPTP-induced PD mouse model was established and animals were divided into 5 groups by random number table method: control group, MPTP group (15, 20, and 30 mg/kg on days 1, 2 and 3-7, respectively), and MPTP+HYP low-, medium-, and high-dose groups (25, 50, and 100 mg·kg^-1·d^-1, respectively). Behavioral tests were conducted and protein expression levels of tyrosine hydroxylase (TH), Nrf2, HO-1, B-cell lymphoma 2 (Bcl-2), and Bcl-2-associated X protein (Bax) were analyzed using Western blot. Glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) levels were measured by ELISA. Molecular docking and dynamics simulations were performed on HYP and Nrf2, and neuronal apoptosis was detected by TUNEL assay. Multivariate statistical analysis was used to investigate the correlation between HYP's therapeutic effect on PD and OS via the Nrf2/HO-1 pathway. In the SH-SY5Y cell model (MPP⁺ and ML385), cell viability and toxicity were assessed with cell counting kit-8 and lactate dehydrogenase assays, and protein expression levels and OS indicatars were also measured.</p><p><strong>Results: </strong>In animal experiments, HYP intervention improved motor abilities, increased TH, Nrf2, HO-1, and Bcl-2 expressions, and reduced Bax expression compared to the MPTP group (P<0.05 or P<0.01). It also elevated GSH, GSH-Px, SOD, and CAT levels, decreased MDA, and reduced neuronal apoptosis (P<0.05 or P<0.01). Molecular docking and dynamics showed HYP binds effectively to Nrf2. Multivariate analysis revealed a strong correlation between HYP's therapeutic effect on PD and OS via the Nrf2/HO-1 pathway. In cell experiments, ML385 inhibited Nrf2/HO-1 activation by HYP (P<0.05 or P<0.01), diminishing its effect on OS and apoptosis.</p><p><strong>Conclusion: </strong>HYP can reduce OS and apoptosis by activating the Nrf2/HO-1 pathway, thereby exerting a therapeutic effect in PD.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gancao Ganjiang Decoction Inhibits Renal Cell Carcinoma Progression via PI3K-AKT and JAK-STAT Signaling Pathways.","authors":"Yi-Xue Zhang, Ji Huang, Hui-Ya Chen, Tian Tian, Zhen-Xiao Sun","doi":"10.1007/s11655-026-4155-3","DOIUrl":"https://doi.org/10.1007/s11655-026-4155-3","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the therapeutic potential of Chinese medicine prescription Gancao Ganjiang Decoction (Licorice and Dried Ginger Decoction, LDGD) against renal cell carcinoma (RCC) and to elucidate its underlying mechanisms.</p><p><strong>Methods: </strong>A mouse RCC model was established in BALB/c mice by subcutaneous injection of Renca cells. The effects of LDGD (5, 10, and 15 g/kg), cisplatin (3 mg/kg), and their combination (10 g/kg LDGD + 3 mg/kg cisplatin) on tumor growth were assessed. Ki-67 expression in tumor tissue was detected by immunohistochemistry. The anti-proliferative effects of LDGD and its 7 blood-absorbed constituents were evaluated on 769-P and 786-O human RCC cell lines using MTT and colony formation assays. Network pharmacology was conducted to predict core targets and signaling pathways. Molecular docking was performed to forecast interactions between active components of LDGD and key proteins in related signaling pathways. Western blot was used to validate phosphorylation levels of the key proteins.</p><p><strong>Results: </strong>LDGD and cisplatin inhibited mouse RCC growth in vivo. The combination of LDGD with cisplatin enhanced the tumor inhibition rate as compared with cisplatin alone. Ki-67 expression was reduced following treatments. LDGD and its blood-absorbed constituents inhibited RCC cell viability and proliferation in a time- and dose-dependent manner in vitro. Network pharmacology showed that PI3K-AKT and JAK-STAT pathways implicated in the anti-RCC effect of LDGD and its blood-absorbed constituents, and AKT1 and STAT3 were potential targets. Molecular docking suggested that isoliquiritigenin and 6-shogaol had strong binding affinity for AKT1 and STAT3. Western blot showed that LDGD suppressed phosphorylation of AKT and STAT3 in 769-P cells, while inhibited phosphorylation of STAT3 protein in 786-O cells. Isoliquiritigenin inhibited phosphorylation of AKT and STAT3 proteins in both 769-P and 786-O cells, and 6-shogaol inhibited phosphorylation of STAT3 protein in 786-O cells.</p><p><strong>Conclusion: </strong>LDGD exerts significant anti-tumor effects against RCC, possibly via suppression of AKT and STAT3 phosphorylation.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148444553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}