Chinese Journal of Integrative Medicine最新文献

{"title":"Naoxueshu Oral Liquid Promotes Hematoma Absorption and Relieves Neurological Symptoms in Chronic Subdural Hematoma Patients: A Prospective, Single-Arm, Self-Controlled Study.","authors":"Yan-Ping Tong, Li Li, Xiao-Han Zhang, Wei-Ming Liu, Liang Wu, Wei Wei, Tao Yang, Zhi-Hong Li, Hong-You Fan, Yong-Ping Fan","doi":"10.1007/s11655-026-3958-6","DOIUrl":"10.1007/s11655-026-3958-6","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the potential of Naoxueshu Oral Liquid (NXS) to promote hematoma absorption in patients with chronic subdural hematoma (CSDH).</p><p><strong>Methods: </strong>From September 2020 to August 2023, 60 patients with CSDH confirmed by computed tomography (CT) scan were enrolled in this prospective clinical trial. All patients received NXS at a dose of 10 mL thrice daily for 90 d. The outcomes included changes in maximal hematoma thickness (MHT), scores of Markwalder Grading Scale-Glasgow Coma Scale (MGS-GCS) and Barthel Index (BI) on days 30, 60, 90, and 180 from baseline, as well as the relapse rate by day 180. For subgroup analysis, patients aged over 60 years were divided into an elderly group (60-74 years) and an oldest-old group (> 75 years) to evaluate the effect of NXS in older individuals. Safety was also assessed during the study.</p><p><strong>Results: </strong>All participants were included in the full analysis set (FAS) according to the modified intention-to-treat principle. On day 90, MHT significantly decreased from 16.00 [interquartile range [(IQR) 12.00-24.60] mm to 3.75 (IQR 0-10.00) mm (P<0.01), with complete hematoma absorption observed in 30% (18/60) of patients. MGS-GCS scores on days 90 and 180 and BI on day 180 significantly improved compared with baseline (P<0.05 or P<0.01). The relapse rate on day 180 was 1.5% (1/60) in the FAS. Both subgroups showed significant within-group improvements in MHT, MGS-GCS, and BI (P<0.01). Intergroup differences were noted in MHT, BI, and MGS-GCS at specific time points (P<0.05 or P<0.01). No treatment-related adverse events were reported.</p><p><strong>Conclusions: </strong>NXS may promote hematoma absorption and improve neurological impairment in patients with CSDH. These findings suggest that NXS represents a potentially effective treatment for CSDH. (Trial registration No. ChiCTR2400090565).</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":"829-835"},"PeriodicalIF":3.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gypenosides Induce Preadipocyte Browning via FXR/Rubicon Pathway-Mediated Inhibition of Autophagy.","authors":"Yan-Qiu Wang, Chao Chen, You-Kui Shen","doi":"10.1007/s11655-026-4043-x","DOIUrl":"https://doi.org/10.1007/s11655-026-4043-x","url":null,"abstract":"<p><strong>Objective: </strong>To determine whether gypenosides (GPs) induce preadipocyte browning via famesoid X receptor (FXR)/Rubicon-mediated autophagy inhibition and ameliorate obesity-related metabolic disorders in mice.</p><p><strong>Methods: </strong>In vitro, differentiated 3T3-L1 preadipocytes were treated with 1-6 µ g/mL GPs. Browning markers [uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1 α), PR domain containing 16 (Prdm16)], autophagy markers [microtubule-associated protein 1 light chain 3 form II/form I (LC3II/LC3I), Beclin-1, recombinant autophagy related protein 7 (ATG7), p62], and FXR/Rubicon expression were assessed by Western blot and qPCR. FXR knockdown was performed using shRNA lentivirus. Autophagic flux was evaluated using an mRFP-GFP-LC3 tandem reporter. In vivo, high-fat diet-induced obese mice were randomly divided into 5 groups using a random number sequence (n=6 per group): control, model, low- and high-dose GPs (300, 600 mg/kg per day by oral gavage, respectively), and sitagliptin (SIT, 30 mg/kg daily) groups. All treatments were administered daily for 12 weeks. Metabolic parameters, adipocyte histology, and protein expression in inguinal white adipose tissue were then analyzed.</p><p><strong>Results: </strong>GPs (3-6 µ g/mL) significantly upregulated UCP1, PGC-1α, and Prdm16 protein levels and reduced the LC3II/LC3I ratio compared with the differentiation medium control (all P<0.01). FXR and Rubicon expression were increased by GPs (P<0.01). FXR knockdown abolished these effects (P<0.01). In obese mice, GPs (600 mg/kg) reduced body weight, serum triglycerides, total cholesterol, and fasting blood glucose, decreased adipocyte size, and increased UCP1, PGC-1α, Prdm16, FXR, and Rubicon while decreasing LC3II/LC3I, Beclin-1, and ATG7 compared with the model group (P<0.05).</p><p><strong>Conclusion: </strong>GPs activate FXR/Rubicon signal pathway to inhibit autophagy, inducing preadipocyte browning and offering a novel herbal-based anti-metabolic diseases strategy.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium Danshensu Attenuates Renal Ischemia-Reperfusion Injury by Suppressing STAT3-Driven Cytokine Signaling and Apoptosis.","authors":"Ya-Qi Zhou, Jia-Qi Li, Yi-Jian Sun, Jia-Fan Sun, Song-Lin Zhu, Jin Wang, Jie Sun, Zheng Wang, Na Li","doi":"10.1007/s11655-026-4163-3","DOIUrl":"https://doi.org/10.1007/s11655-026-4163-3","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the renoprotective effects and underlying molecular mechanisms of sodium danshensu (SDSS) in renal ischemia-reperfusion (RIR) induced acute kidney injury.</p><p><strong>Methods: </strong>Network pharmacology, Weighted Gene Co-expression Network Analysis, single-cell RNA sequencing and machine learning algorithms were integrated to systematically identify SDSS's potential therapeutic targets in RIR. Molecular docking was also employed to confirm key target interactions. Target validation was performed both in vivo and in vitro. In vivo, a rat RIR model was established by occluding bilateral renal arteries, and SDSS (20 mg•kg^-1•d^-1) was administered intraperitoneally for 28 d. Renal injury was evaluated by HE and TTC stainings, while the expression of signal transducer and activator of transcription 3 (STAT3), phosphorylated STAT3 (p-STAT3), BCL2 associated X (BAX), and caspase 3 (CASP3) in kidney tissues was detected via immunofluorescence, Western blot, and qRT-PCR. In vitro, hypoxia/reoxygenation-treated HK-2 cells were used to assess the protective effect of SDSS (13 µg/mL), with cell viability and apoptosis measured by cell counting kit-8 and flow cytometry, respectively, and STAT3 phosphorylation levels examined by Western blot, further confirming the involvement of the STAT3 pathway.</p><p><strong>Results: </strong>The results of network pharmacology and multiomics analysis identified STAT3, P-STAT3, RELA proto-oncogene (RELA), C-C motif chemokine ligand 2 (CCL2), tumor necrosis factor (TNF), BAX, and CASP3 as therapeutic targets involved in inflammation, apoptosis and immune regulation during RIR progression. More importantly, SDSS significantly reduced the infarction volume and preserved tissue integrity in vivo (P<0.01). RIR upregulated STAT3, BAX, and CASP3 expressions, which SDSS treatment effectively reversed both in vivo and in vitro (P<0.01).</p><p><strong>Conclusion: </strong>SDSS alleviates RIR injury by suppressing inflammatory and apoptotic signaling, primarily through downregulation of STAT3, BAX, and CASP3, providing new insights into its therapeutic mechanism and potential clinical application.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148839461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yinchenhao Decoction Alleviates Liver Injury in CYP2D6-Induced Autoimmune Hepatitis Mice: An Integrated Transcriptomic and Proteomic Analysis.","authors":"Na Yang, Fen-Qing Cai, Hui-Yue Tao, Di Guo, Jia-Wen Wu, Yang Liu","doi":"10.1007/s11655-026-3856-y","DOIUrl":"https://doi.org/10.1007/s11655-026-3856-y","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the therapeutic effects of Yinchenhao Decoction (YCHD) on a mouse model of autoimmune hepatitis (AIH) induced by cytochrome P450 2D6 adenovirus (Ad-CYP2D6), and reveal its potential molecular mechanisms through integrated transcriptomic and proteomic analyses.</p><p><strong>Methods: </strong>AIH model was established via tail vein injection of 100 µL Ad-CYP2D6 (1 × 10⁹ PFU/mL) to C57BL/6 mice. Seven days post-injection, the mice were stratified by weight and randomly divided into 3 groups (n=6-8 per group), including the normal control, AIH model, and YCHD treatment groups. YCHD treatment group received daily oral gavage of YCHD at a dosage of 10 mL/kg for 2 consecutive weeks, while the normal and model groups received an equivalent volume of normal saline. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured and anti-liver kidney microsomal-1 (LKM-1) autoantibody was detected by ELISA. Liver histopathology was assessed using HE staining. RNA sequencing (RNA-seq) and tandem mass tag (TMT) proteomics were employed to analyze the differential gene and protein expressions in liver tissues, with validation via qPCR and Western blot.</p><p><strong>Results: </strong>Compared with the normal control group, the model group showed significantly increased liver and spleen indices and markedly elevated serum ALT and AST levels, which were markedly reduced following YCHD treatment (P<0.05 or P<0.01). HE staining revealed prominent inflammatory cell infiltration and hepatocyte nuclear loss in the model group; these pathological alterations were significantly ameliorated in the YCHD treatment group. Serum level of specific autoantibody LKM-1 was increased in the model group and significantly reduced following YCHD treatment (P<0.05). Integrated transcriptomic and proteomic analyses demonstrated signal transducer and activator of transcription 1 (Stat1), guanylate-binding protein 3 (Gbp3), and T-cell-specific guanosine triphosphatase 2 (Tgtp2) were significantly upregulated in the model group and were significantly downregulated after YCHD treatment (P<0.05 or P<0.01).</p><p><strong>Conclusions: </strong>YCHD effectively alleviates liver injury in Ad-CYP2D6-induced AIH mice. Its underlying mechanism may be associated with the suppression of interferon-β cellular response pathway mediated by Stat1, Gbp3, and Tgtp2.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148839391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cuproptosis: New Perspectives for Prevention and Treatment of Osteoarthritis with Chinese Medicine Extracts.","authors":"Yu Chen, Ting Qin, Zheng-Zhen Wang, Guo-Dong Huang, Fang Tang","doi":"10.1007/s11655-026-4049-4","DOIUrl":"https://doi.org/10.1007/s11655-026-4049-4","url":null,"abstract":"","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spinal Manipulation Therapy Remodels CA1-Centered Default Mode Network to Alleviate Comorbid Low Back Pain and Depression-Like Behaviors in Rats.","authors":"Xing-Chen Zhou, Qiang Hu, Shan-Yan Lei, Zhao Yao, Li-Jiang Lyu, Zuo-Bing Chen","doi":"10.1007/s11655-026-4169-x","DOIUrl":"https://doi.org/10.1007/s11655-026-4169-x","url":null,"abstract":"<p><strong>Objective: </strong>To explore neuroimaging biomarkers associated with alleviation of both pain and negative emotions among rats with low back pain (LBP) that received spinal manipulation therapy (SMT).</p><p><strong>Methods: </strong>Forty Sprague-Dawley rats were randomly allocated to the control, model, SMT, and sham-SMT groups, with 10 rats in each group. A LBP rat model was established by chronically compression of dorsal root ganglion. The rats underwent 7.0 T magnetic resonance imaging (MRI) scans before and after SMT. Pain and negative emotions were also measured. Structural and functional MRI data were analyzed using network-based statistic (NBS) correction to identify regions of interest (ROIs) with significant differences in connectivity, and the Pearson's correlations between these ROIs and behavioral data were examined.</p><p><strong>Results: </strong>After SMT, pain and depression-like behaviors were alleviated in LBP rats, accompanied by concordant alterations in regional spontaneous brain activity. Subsequent zFC analysis with NBS correction revealed significant connectivity alterations involving left cornu ammonis 1 (CA1_L), right cornu ammonis 1 (CA1_R), right lateral entorhinal cortex (LEC_R), and left primary visual cortex, binocular area (V1B_L). Positive correlations were observed between CA1_R-related connectivity and paw withdrawal latency (r=0.409, P=0.024) and between CA1_L-related connectivity and paw withdrawal threshold (r=0.371, P=0.029). In contrast, the depression index was negatively correlated with connectivity involving V1B_L, CA1_R, LEC_R, and CA1_L (r=-0.326, -0.314, -0.336, and -0.347, respectively, all P<0.05). No significant correlations with anxiety-related measures were identified.</p><p><strong>Conclusion: </strong>The neurofunctional remodeling of the default mode network, which is centered around the CA1 region, is likely a key neuroimaging biomarker for regulating comorbid pain and depression-like behaviors in LBP rats after SMT.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compound Kushen Injection Mitigates Radiation-Induced Pulmonary Fibrosis by Restoring AT2 Cell Stemness and Alveolar Regenerative Capacity.","authors":"Yan-Li Liu, Cheng-Yan Xu, Yong Wang, Xiao-Yan Liang, Yun Chen","doi":"10.1007/s11655-026-4159-z","DOIUrl":"https://doi.org/10.1007/s11655-026-4159-z","url":null,"abstract":"<p><strong>Objective: </strong>To determine whether radiation directly compromises AT2 cell stemness through oxidative and DNA damage, and to evaluate whether Compound Kushen Injection (CKI) protects AT2 cells, restores alveolar regeneration, and mitigates radiation-induced pulmonary fibrosis.</p><p><strong>Methods: </strong>Mice were randomly assigned to a control, irradiated model, or CKI-treated group. CKI (4 mL·kg^-1·d^-1) was administered intraperitoneally from 3 days before irradiation until the designated endpoints. A thoracic irradiation plus left pneumonectomy model was established to mimic clinical scenarios requiring postoperative lung regeneration. Lung function, histopathology, and compensatory growth of the accessory lobe were assessed. AT2 cells proliferation, differentiation, and transitional Krt8⁺ accumulation were evaluated using lineage tracing in Sftpc-CreER and Rosa26-ZsGreen mice. Oxidative stress and DNA damage were examined by immunofluorescence. Transcriptomic profiling of sorted lineage-labeled AT2 cells was conducted to identify radiation- and CKI-responsive genes.</p><p><strong>Results: </strong>Radiation caused marked oxidative stress and γ H2AX-defined DNA damage in AT2 cells, accompanied by suppression of Sod1 and Parp1 and increased Il6 and Tgfb1 expression (P<0.01). These epithelial-intrinsic injuries led to reduced AT2 proliferation, impaired AT2-to-AT1 differentiation, and long-term accumulation of Krt8⁺ transitional cells. Functionally, irradiation significantly impaired compensatory lung regeneration after pneumonectomy and promoted fibrotic remodeling. CKI markedly alleviated ROS accumulation and DNA damage in AT2 cells, restored Sod1 and Parp1 expression, suppressed inflammatory and profibrotic signaling, rescued AT2 proliferative and differentiative capacities, reduced transitional Krt8+ cell accumulation, and improved both alveolar regeneration and fibrosis (P<0.05).</p><p><strong>Conclusions: </strong>CKI preserves epithelial integrity by reducing oxidative and DNA damage, restoring AT2 stemness, and enhancing alveolar repair. These findings provide mechanistic support for CKI as a potential epithelial-directed therapeutic strategy for preventing or treating radiation-induced lung fibrosis.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148765058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chinese Medicine Reduces Treatment Failure in Elderly Patients with Severe Community-Acquired Pneumonia: A Post-Hoc Analysis of a Randomized Controlled Trial.","authors":"Hai-Feng Wang, Kang Zhang, Kai Xie, Hao Yang, Wen-Shuai Ji, Jian-Sheng Li","doi":"10.1007/s11655-026-3960-z","DOIUrl":"https://doi.org/10.1007/s11655-026-3960-z","url":null,"abstract":"<p><strong>Objective: </strong>To explore the effectiveness and safety of Chinese medicine (CM) treatment based on pattern identification (PI) in elderly patients (⩾ 60 years) with severe community-acquired pneumonia (CAP).</p><p><strong>Methods: </strong>This post hoc analysis was based on a multicenter, randomized, placebo-controlled trial. Elderly patients with severe CAP were randomly assigned to receive either conventional medicine plus CM placebo (conventional group) or conventional medicine combined with CM treatment based on PI (combination group) for a 28-d treatment period. The primary outcome was the rate of treatment failure. Secondary outcomes included 28- and 90-d mortality, time to clinical stability, rate of clinical stability, length of hospital stay, and adverse events.</p><p><strong>Results: </strong>A total of 128 elderly patients with severe CAP were included in the intention-to-treat population, comprising 67 patients in the combination group and 61 in the conventional group. The rate of treatment failure was lower in the combination group than in the conventional group (20.9% vs. 39.3%), with a between-group difference of 18% [95% confidence interval (CI), 0.03-0.34; P<0.05]. The 28-d mortality in the combination group was lower than that in the conventional group (19.4% vs. 36.1%), with a difference of 17% (95% CI, 0.01-0.32; P<0.05). Time to clinical stability was significantly shorter in the combination group than in the conventional group, with a Hazard ratio of 2.44 (95% CI, 1.44-4.14; P<0.01). The rate of clinical stability was higher in the combination group than in the conventional group (64.2% vs. 34.4%), with the between-group difference was 30% (95% CI, 0.13-0.46; P<0.01). Similar results were observed in the per-protocol population. The incidence of adverse events was similar between combination and conventional groups.</p><p><strong>Conclusion: </strong>The combination of conventional medicine with CM treatment based on PI was effective and safe in reducing treatment failure among elderly patients with severe CAP compared with conventional medicine alone.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148764496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ruanjian Sanjie Formula Affects Metastasis of Lung Cancer by Regulating miR-182-5p/EPAS1/EMT Axis.","authors":"Guan-Jin Wu, Ling Xu, Yi-Nan Yin, Zong-Mei Zheng, Xu Wang, Ling Bi, Qin Wang, Jia-Lin Yao, Wen-Xiao Yang, Li-Jing Jiao","doi":"10.1007/s11655-026-4157-1","DOIUrl":"https://doi.org/10.1007/s11655-026-4157-1","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the specific mechanism by which Ruanjian Sanjie Formula (RJSJ) inhibits the occurrence and metastasis of lung cancer.</p><p><strong>Methods: </strong>This study developed an in vivo model using H460 cells in nude mice to investigate postoperative lung cancer metastasis. Two groups received either RJSJ (0.2 mL) or saline to assess RJSJ's anti-cancer effects, including disease-free survival duration, recurrence-free metastasis rate, number of pulmonary metastases, as well as weight and coefficient of major organs. An in vitro study was conducted to evaluate the effects of RJSJ at concentrations ranging from 0 to 500 µg/mL on the proliferation, migration, and invasion capabilities of H460 and H1975 cell lines. These effects were assessed using the CCK-8 assay for cell proliferation, the wound healing assay for cell migration, and the Transwell assay for cell invasion. RJSJ's chemical profile was analyzed via UPLC-Q-TOF-MS, and its mechanism was explored through network pharmacology. Specific microRNAs and their target genes were identified and analyzed using bioinformatics, qRT-PCR, and Western blot to examine RJSJ's impact on metastasis-related genes and proteins during epithelial-mesenchymal transition (EMT). Virtual docking and molecular simulations were used to evaluate interactions between RJSJ compounds and microRNAs.</p><p><strong>Results: </strong>RJSJ could inhibit the proliferation, migration, and invasion of lung cancer cells in vitro (P<0.05 or P<0.01), and it had also been shown to prevent lung cancer recurrence and metastasis in vivo (P<0.05); it contains 47 chemical compounds that act on 191 common targets, including 36 core targets. It reduced cancer cell proliferation and invasion, downregulated miR-182-5p, thereby regulating its target gene EPAS1, and regulated EMT-related markers by decreasing the expression of N-cadherin, MMP2, MMP9, Snail, and Twist, while increasing the expression of E-cadherin. Chlorogenic acid was identified as the main anti-tumor component due to its strong binding affinity to nucleic acids.</p><p><strong>Conclusions: </strong>RJSJ inhibits lung cancer occurrence and metastasis both in vivo and in vitro. Mechanistically, RJSJ suppresses the EMT process by downregulating miR-182-5p and inhibiting EPAS1 expression, thereby restraining lung cancer malignant progression, with chlorogenic acid identified as a key active component.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Eucommia ulmoides Oliv. on Key Nodes and Gene Expression in Wnt/β-Catenin Pathway in Collagen-Induced Arthritis Rats.","authors":"Tian-Qi Jia, Ruo-Yan Yang, Ying-Yuan Chen, Jian-Ying Wang, Jia-Yi Tao, Zu-Xi Gu, Lei Zhang, Ying Yuan","doi":"10.1007/s11655-026-4047-6","DOIUrl":"https://doi.org/10.1007/s11655-026-4047-6","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the therapeutic mechanisms of Eucommia ulmoides Oliv. (EC) in a collagen-induced arthritis (CIA) rat model.</p><p><strong>Methods: </strong>The CIA model was established by immunizing rats with type II collagen. Wistar rats were assigned to the normal group (n=6), and the remaining model rats were further divided by body weight into the CIA model, EC, and Iguratimod (IGU) positive control groups (n=6 per group). EC (4 g/kg per day) and IGU (4.5 mg/kg daily) was administered orally for 4 weeks. Arthritis severity was evaluated by measuring hind paw volume. Histopathological analysis of ankle joints was performed using hematoxylin and eosin (HE) and safranin O/fast green. Serum levels of procollagen type I N-terminal propeptide (PINP), cross-linked carboxy-terminal telopeptide of type I collagen (ICTP), osteocalcin (BGP), β-crosslaps (β-CTX), tumor necrosis factor (TNF-α), interleukin (IL)-1β, and CTNNB1 (β-catenin) were measured by ELISA. Serum nitric oxide (NO) levels were measured using a nitrate reductase assay. Transcriptomic sequencing and RT-qPCR were performed to assess mRNA expression, and Western blot was used to assess protein expression of Wnt2 and β-catenin in the spleen.</p><p><strong>Results: </strong>Compared with the CIA group, EC significantly alleviated arthritis severity and paw swelling (P<0.05) and reduced synovial inflammation and cartilage damage. Serum analysis showed that EC decreased TNF-α, IL-1β, NO, ICTP, and β-CTX, while increasing PINP and BGP (P<0.05 or P<0.01), and also reduced CTNNB1 (P<0.01) compared with the CIA group. In the spleen, EC reduced mRNA expressions of IL-1β, IL-6, and TNF-α (P<0.05 or P<0.01), upregulated IL-10, and decreased β-catenin protein (P<0.05); Wnt2 mRNA showed a non-significant downward trend compared with the CIA group. In the ankle joints, EC significantly reduced mRNA expressions of Wnt2 and β-catenin (P<0.05 or P<0.01).</p><p><strong>Conclusions: </strong>EC may alleviate inflammation and bone destruction in RA by regulating the Wnt/β-catenin signaling pathway, suggesting its potential as a therapeutic agent for RA.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}