B. S. C. Barcellos, C. B. Agustini, G. S. Rolim, João H. Z. Santos, M. Gutterres
{"title":"Carbon and nitrogen removal by Chlorella sp. and Scenedesmus sp. suspended and immobilised in non-toxic silica gel in leather processing wastewater","authors":"B. S. C. Barcellos, C. B. Agustini, G. S. Rolim, João H. Z. Santos, M. Gutterres","doi":"10.1080/02757540.2023.2199725","DOIUrl":"https://doi.org/10.1080/02757540.2023.2199725","url":null,"abstract":"ABSTRACT Microalgae have been effectively used as a nutrient removal method in wastewater treatment. However, the harvesting microalgae step is a major hurdle to the industry. In this study, the silica non-toxic sol–gel is used as a matrix to immobilise microalgae. Non-toxic silica gel matrix was synthesised by tetraethoxysilane (TEOS) derived alcohol free route. This study aims to evaluate the effect of microalgae immobilisation in non-toxic silica gel to remove tanneries wastewater pollutants. The experiments were conducted by applying immobilised Scenedesmus sp. and Chlorella sp. in three concentrations in leather processing wastewater during 72 h of cultivation. The morphology of immobilisation was evaluated by optical microscopy, SEM and Zeta Potential analysis. The rate removals of total nitrogen (TN), total organic carbon (TOC) and Inorganic Carbon (IC) in comparison to immobilised and suspended microalgae were evaluated by the influence of the immobilisation, type of microalgae and microalgae concentration. For both microalgae, immobilisation showed promise for removing these nutrients. The immobilised Chlorella sp. at the initial concentration of 5.84 ± 0.975 mg.L−1 (I-Ch2) had the highest removal by immobilised microalgae, with an uptake of 20.82% of AN, 18.44% of TN, 30.42% of TOC and 64.73% of IC.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"438 - 458"},"PeriodicalIF":2.5,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44788103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nutrient and algal responses to a managed drawdown in an agricultural riverine lake","authors":"R. Lizotte, J. Murdock, J. M. Taylor, M. Locke","doi":"10.1080/02757540.2023.2198511","DOIUrl":"https://doi.org/10.1080/02757540.2023.2198511","url":null,"abstract":"ABSTRACT Water level of a shallow impounded riverine lake was lowered to help improve downstream flows and water quality within the Big Sunflower River in the agriculturally dominated Mississippi Delta, Mississippi, USA. A study was conducted to better understand how nutrient and algal processes within shallow lakes respond to relatively rapid water level changes to guide water resource management decisions. The drawdown was initiated in late summer and completed within six days, ending when outflow was <5% of peak flows. Water depths were reduced from 28-82%. Nutrients (phosphorus, nitrogen and carbon) and water column algal measurements (chlorophyll a, in-vivo chlorophyll, in-vivo phycocyanin, chlorophyll:phycocyanin ratio, and photosynthetic activity) were measured at three sites on days −7, −1, 0, 1, 2, 3, 6, 13, 20, 28, and 34, relative to the start of the drawdown. Nitrogen and phosphorus responses were bimodal, showing a decrease during the initial drawdown phase followed by increasing levels in the ensuing weeks. Algal responses showed complex interactions between nutrients and changes in light availability that were often indirectly related to hydrologic changes. Late summer to early fall drawdowns in small impoundments used to improve downstream flows can have minimal water quality impacts on the upstream impounded systems.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"319 - 336"},"PeriodicalIF":2.5,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45376842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tianxu Zhang, Yan Song, Weiying Xu, Nannan Lu, Yan Chen, R. Jia, Shaohua Sun
{"title":"Individual and combined toxicity of sulfamethoxazole and desethylatrazine to Chlorella vulgaris: growth inhibition, photosynthetic activity and oxidative stress","authors":"Tianxu Zhang, Yan Song, Weiying Xu, Nannan Lu, Yan Chen, R. Jia, Shaohua Sun","doi":"10.1080/02757540.2023.2189893","DOIUrl":"https://doi.org/10.1080/02757540.2023.2189893","url":null,"abstract":"ABSTRACT The influence of emerging contaminants on the composition of aquatic ecosystems, including antibiotics and pesticide metabolites, is receiving increasing attention. In this study, the acute and chronic toxic effects of single and combined exposure to sulfamethoxazole (SMX) and desethylatrazine (DEA) on Chlorella vulgaris (C. vulgaris) were evaluated in terms of growth, pigment content and antioxidant systems. The findings demonstrated that cytochrome content and cell growth followed a similar trend for both single and combined exposures, with C. vulgaris being affected in a dose-dependent manner, and chlorophyll content decreasing at high exposures. For SMX and DEA, the maximum inhibition rates at a single exposure were 95.14% and 87.31%, respectively. Cell membrane permeability, superoxide dismutase content and catalase release were significantly increased in the early stages of cultivation. Additionally, cell density was used to calculate the single exposure half effect concentration (EC50), which was then used as part of the toxic unit (TU) method for evaluating mixture interactions. TU analysis revealed that the interaction between SMX and DEA shifted from being synergistic to being antagonistic after 7 d of incubation. These results suggest that SMX and DEA may play a significant role in aquatic ecosystems. GRAPHICAL ABSTRACT","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"337 - 356"},"PeriodicalIF":2.5,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49206877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thendo Mutshekwa, L. Mugwedi, Babra Moyo, N. Madala, R. Wasserman, F. Dondofema, T. Dalu
{"title":"Assessing acetamiprid and chlorpyrifos pesticide concentrations in water and sediments across macadamia orchard and communal area small reservoirs","authors":"Thendo Mutshekwa, L. Mugwedi, Babra Moyo, N. Madala, R. Wasserman, F. Dondofema, T. Dalu","doi":"10.1080/02757540.2023.2199015","DOIUrl":"https://doi.org/10.1080/02757540.2023.2199015","url":null,"abstract":"ABSTRACT Pesticides are well known for their persistence in the environment their slow degradation and high toxicity. Aquatic environments, while not the target of many pesticide applications, often receive these chemicals from their catchments through runoff dynamics. Here, we assessed pesticide concentrations of acetamiprid and chlorpyrifos in water and sediment across macadamia orchard and communal area reservoirs in the Levubu area, South Africa, using liquid chromatography tandem mass spectrometry. Analysis of acetamiprid and chlorpyrifos associated with sediments in macadamia orchard reservoirs revealed mean pesticide concentrations of 14.48 µg L−1 and 5.67 µg L−1, respectively, whereas, in communal area reservoirs both pesticides were not detected. Acetamiprid was not detected across reservoir water, whereas the mean pesticide concentration of chlorpyrifos of 6.51 µg L−1 (macadamia orchard) and 0.13 µg L−1 (communal area) were detected. Significant differences were observed for chlorpyrifos concentrations in water samples between macadamia orchard and communal area reservoirs, whereas acetamiprid and chlorpyrifos indicated non-significant differences in sediments. The results indicate that macadamia orchard reservoirs are highly contaminated by acetamiprid and chlorpyrifos. Consequently, these pesticides can alter water quality and ecosystem functioning by reducing species diversity, adjusting community structure, altering energy flow and nutrient recycling.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"393 - 403"},"PeriodicalIF":2.5,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46255984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploration of haematite-loaded rice husk biochar as a low-cost nanosorbent to remove Cr (III) from the aqueous media","authors":"T. Iqbal, Fozia Batool, S. Iqbal","doi":"10.1080/02757540.2023.2188205","DOIUrl":"https://doi.org/10.1080/02757540.2023.2188205","url":null,"abstract":"ABSTRACT In this study, the biochar of rice husk was modified to a nanosorbent upon loading with haematite for Cr (III) removal from aqueous solutions. Three adsorbents, rice husk, rice husk biochar and haematite-loaded rice husk biochar, were used for equilibrium adsorption studies. SEM and FTIR analysis revealed the presence of pores and various surface active sites on a nanomaterial, which were responsible for the adsorption of Cr (III). C–H, –O–H, C=O stretching vibrations were observed in characterised FTIR peaks. Modelling of adsorption isotherms indicated best fit for Freundlich isotherm with maximum Kf value of 12,218 and 39.5 for linear and non-linear nanomaterial experimental calculations, and positive n value in range of 1–10 for all forms of adsorbents, Dubinin–Radushkevich adsorption saturation capacity qs showed high readings of 9417 and 76.8 for haematite nanomaterial, Elovich isotherm favoured non-linear results in case of nanomaterial with increased Qm values 43.4–112.3 from raw form to nanosorbents and Temkin isotherm with. Kinetic and Thermodynamic results were also summarised. Constants k and h and Qe capacity were tabulated for Pseudo second order model with highest Qe in the range of 20.36–22.52 for haematite loaded biochar nanomaterial. Hence, haematite based rice husk biochar nanostructure was employed as an efficient adsorbent for uptake of chromium.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"404 - 421"},"PeriodicalIF":2.5,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46632456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Otávio Augusto Lacerda Ferreira Pimentel, N. H. They, Rildo José Vasconcelos de Andrade, Valdemir Queiroz de Oliveira, A. Amado, A. Gálvez, L. O. Brito
{"title":"Ionic adjustments do not alter plankton composition in low salinity Penaeus vannamei intensive nursery with synbiotic system","authors":"Otávio Augusto Lacerda Ferreira Pimentel, N. H. They, Rildo José Vasconcelos de Andrade, Valdemir Queiroz de Oliveira, A. Amado, A. Gálvez, L. O. Brito","doi":"10.1080/02757540.2023.2186405","DOIUrl":"https://doi.org/10.1080/02757540.2023.2186405","url":null,"abstract":"ABSTRACT The aim of this study was to test the effect of different ionic adjustments in low salinity water on the composition and temporal variation of plankton from intensive shrimp nurseries with a synbiotic system. For this, a Penaeus vannamei nursery (35 days) was carried out with three treatments: T1 – diluted seawater (control; salinity ∼2.5 g L−1), T2 – salinity ∼2.5 g L−1 with K+ adjustment to approximate the seawater equivalent concentration, and T3 – salinity ∼2.5 g L−1 with Ca:Mg:K ratio adjusted to 1:3:1. Rice bran processed by probiotic microorganisms was used as an organic carbon source. Phytoplankton and zooplankton were sampled weekly and analysed using standard methods. The temporal variation of phytoplankton and zooplankton composition were more pronounced than differences among treatments indicating that the ionic adjustment had little effect on these communities. During the experimental time, the dominant phyla in phytoplankton were Ochrophyta, Cyanophyta and Chlorophyta, whereas zooplankton’s dominant phyla were Ciliophora, Amoebozoa, and Cercozoa. Cyanophyta’s relative abundance was lower than traditional biofloc systems, suggesting a higher control of these microorganisms in synbiotic systems. Ionic adjustments have then a low potential to affect plankton, likely because limitation by these ions was not achieved under the ionic manipulations tested.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"357 - 375"},"PeriodicalIF":2.5,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46274276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shubhajit Saha, Nimai Chandra Saha, A. Chatterjee, P. Banerjee, Pramita Garai, Pramita Sharma, L. Patnaik, Susri Nayak, K. Dhara, A. Chukwuka, C. Faggio
{"title":"Integrated multi-biomarker responses in Mozambique tilapia, Oreochromis mossambicus under acute and chronic Diazinon® exposures","authors":"Shubhajit Saha, Nimai Chandra Saha, A. Chatterjee, P. Banerjee, Pramita Garai, Pramita Sharma, L. Patnaik, Susri Nayak, K. Dhara, A. Chukwuka, C. Faggio","doi":"10.1080/02757540.2023.2178649","DOIUrl":"https://doi.org/10.1080/02757540.2023.2178649","url":null,"abstract":"ABSTRACT\u0000 The pesticide ecosystem impacts are very diverse, and depending on their persistence could exert their intrinsic toxicity within an acute to a chronic timeframe. This study examined the toxic effects of Diazinon on haematological and biochemical endpoints in Oreochromis mossambicus after chronic exposure. After a prior range fining test, fishes were exposed to predetermined diazinon concentrations (T1-0.906 mg/L) and (T2-1.812 mg/L) and sampled after 28 days for toxicity endpoints. In Diazinon-exposed fish, haemoglobin content, Total Red Blood Cells, Total White Blood Cells, and MCH levels were substantially lower (p < 0.05) than in the control fish group. The total blood glucose concentrations in treated fish were substantially greater (p < 0.05) than in the control group, whereas serum protein concentrations were significantly lower (p < 0.05). Observation for most biomarkers revealed patterns of decreasing values with increasing toxicant concentration and exposure duration. The integrated indices (IBR and BRI) indexes were used to provide visual and understandable depictions of toxicity effects and emphasised the relativity of biomarkers in terms of sensitivity and magnitude or severity of responses under graded toxicant exposures. In acute and chronic diazinon exposure circumstances, the analysed metrics show significant impairment to Oreochromis mossambicus.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"235 - 255"},"PeriodicalIF":2.5,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45317965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Stafilov, R. Šajn, Ana Puteska, Bojana Dimovska
{"title":"Moss biomonitoring of air pollution with potentially toxic elements in the Pelagonia Region, North Macedonia","authors":"T. Stafilov, R. Šajn, Ana Puteska, Bojana Dimovska","doi":"10.1080/02757540.2023.2178650","DOIUrl":"https://doi.org/10.1080/02757540.2023.2178650","url":null,"abstract":"ABSTRACT This study aims to investigate air deposition and explore the distribution and contamination with potentially toxic elements (PTEs) in the Pelagonia Region, North Macedonia. For this goal, 63 moss samples were collected and analysed by inductively coupled plasma – atomic emission spectrometry to determine the content of 19 major and trace elements. It was found that the atmospheric deposition of some PTEs in the moss samples from specific parts of the study area is influenced by anthropogenic activities. Three factor associations were obtained from multivariance R-method factor analysis: Factor 1 (Al, Ba, Cr, Cu, Fe, Li, Ni, V, and Na) mixed lithogenic and anthropogenic association, Factor 2 (Ca, K, Mg, Na, Cd, and Zn) mostly as a result of complex geology and lithology of the region, and Factor 3 (Pb, Zn, and Ni) which represents an anthropogenic association of the elements mostly present in the air particles emitted from the fly ash landfill of the thermoelectric power plant near the city of Bitola. The external influence of the transport of the particles reached in Ni from the ferronickel smelter plant near the town of Kavadarci was also observed in the northeastern part of the study area.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"302 - 318"},"PeriodicalIF":2.5,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49053804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. K. Maneesh Kumar, A. Kartha, S. Rajathy, C. S. Ratheesh kumar, P. Chandini, P. Vidya, P. K. Madhuraj, K. S. Sajith, S. Anamika
{"title":"Characterization of suspended microplastics in surface waters of Chalakudy River, Kerala, India","authors":"S. K. Maneesh Kumar, A. Kartha, S. Rajathy, C. S. Ratheesh kumar, P. Chandini, P. Vidya, P. K. Madhuraj, K. S. Sajith, S. Anamika","doi":"10.1080/02757540.2023.2178648","DOIUrl":"https://doi.org/10.1080/02757540.2023.2178648","url":null,"abstract":"ABSTRACT The investigation focused on identification and quantification of microplastics in surface waters of Chalakudy River, Kerala. Microplastics were detected in the surface waters of the study area with a recovery rate of 108.33 mg particles L−1. The abundance of microplastics ranged from 15.13 ± 4.13 – 170 ± 17.75 particles L−1 (mean: 55.81 ± 34 particles L−1). A maximum number of microplastics were obtained from site C3 and the least from site C8. The weight distribution of microplastic on sites exhibited strong positive correlation with the abundance (r = 0.83). Microplastics of size <20 µm were in higher abundance in the study area. Microscopic examination revealed fragments (47.06%) as predominant plastics and the dominant colour was brown/mud (26.99%). Abundance of coloured plastics indicated the probability of damage to aquatic life. Raman spectrum analysis revealed that Low-Density Poly Ethylene (40%) as the predominant polymer that could be observed at all the sampling sites. Lower-sized microplastics would cause accidental ingestion by organisms. The estimated release of a higher average number of microplastic particles (55.81 ± 4.25) into the marine environment is of serious concern. The observed overall abundance and recovery rate of microplastics in the study area indicated the status of contamination due to a multitude of sources.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"268 - 287"},"PeriodicalIF":2.5,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49006770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amal Lassoued, Noureddine Khalloufi, Wiem Saidani, A. Khazri, N. Ghanem-Boughanmi, J. Bouayed, M. Ben-Attia
{"title":"Effects of increased salinity on oxidative stress status in the freshwater mussel Unio ravoisieri","authors":"Amal Lassoued, Noureddine Khalloufi, Wiem Saidani, A. Khazri, N. Ghanem-Boughanmi, J. Bouayed, M. Ben-Attia","doi":"10.1080/02757540.2022.2163238","DOIUrl":"https://doi.org/10.1080/02757540.2022.2163238","url":null,"abstract":"ABSTRACT Freshwater salinisation is an environmental challenge that threatens freshwater species survival. We investigated the influence of increased water salinity on the oxidative status in the bivalve Unio ravoisieri. Under laboratory conditions, 3 sodium chloride (NaCl) concentrations (2.5, 5 and 7.5) were tested for 7 days compared to a control salinity concentration (0.7), mimicking sampling site condition. Biomarkers of oxidative stress including superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) and malondialdehyde (MDA) were assessed in gills and digestive gland. Furthermore, acetylcholinesterase (AChE) activity was assessed, as a marker of neurotoxicity. Findings showed that the highest concentration of salinity has significantly disturbed oxidative metabolism in both gills and digestive gland by modulating the activity of CAT and the levels of GSH and MDA compared to the control condition (all p < 0.05). Moreover, significant enhance of AChE activity has been found in both gills and digestive gland with the three concentrations (all p < 0.05), except for 2.5 in gills (p > 0.05) compared to control condition. Overall, our study has shown that water salinity constitutes a serious challenge for Unio ravoisieri, and that CAT activity could be used as a sensitive marker for the evaluation of the effect of freshwater salinisation on freshwater.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"39 1","pages":"256 - 267"},"PeriodicalIF":2.5,"publicationDate":"2023-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41386569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}