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Freeze-drying of red blood cells using ultrafast cooling by spraying into a vacuum 冷冻干燥红细胞使用超高速冷却喷涂到真空中
4区 化学
Chemistry Letters Pub Date : 2023-10-14 DOI: 10.1246/cl.230348
Tomoka Konoo, Sora Uematsu, Tsutomu Nishihashi, Hirohiko Murakami, Hidemine Honda, Tsuyoshi Takiuchi, Tadashi Kimura
{"title":"Freeze-drying of red blood cells using ultrafast cooling by spraying into a vacuum","authors":"Tomoka Konoo, Sora Uematsu, Tsutomu Nishihashi, Hirohiko Murakami, Hidemine Honda, Tsuyoshi Takiuchi, Tadashi Kimura","doi":"10.1246/cl.230348","DOIUrl":"https://doi.org/10.1246/cl.230348","url":null,"abstract":"Ultrafast cooling inhibits the growth of ice crystals and prevents red blood cells from being damaged by ice crystals. Micro-Powder-Dry(μPD), a novel freeze-drying technique, was applied in this work. Using the µPD approach, we looked at the hemolysis and morphology of frozen/freeze-dried red blood cells. After freezing, the µPD method had a higher recovery rate than the slow freezing method. The recovery of the red blood cells was maintained after freeze-drying. This study aims to develop freeze-dried red blood cells using a novel spray freeze-drying mathod called µPD(micro-powder-dry). In the µPD process, the solution is injected into a vacuum. Then the droplets self-freeze due to the decrease in temperature, forming ice particles. Lastly, they are heated and dried under a vacuum. The µPD powder is porous and exhibits excellent solubility.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135766102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Calixarene-based Cage Compound: Synthesis via Simple Condensation of t-Butylcalix[4]arene with α,ω-Dibromoacetate and Inclusion Complex Formation with Phenol 杯芳烃基笼状化合物:杯芳烃与α，ω-二溴乙酸酯简单缩合合成及与苯酚形成包合物
4区 化学
Chemistry Letters Pub Date : 2023-10-13 DOI: 10.1246/cl.230377
Hiroyuki Maekawa, Tatsuo Yajima, Hiroto Kudo
{"title":"Calixarene-based Cage Compound: Synthesis via Simple Condensation of <i>t</i>-Butylcalix[4]arene with α,ω-Dibromoacetate and Inclusion Complex Formation with Phenol","authors":"Hiroyuki Maekawa, Tatsuo Yajima, Hiroto Kudo","doi":"10.1246/cl.230377","DOIUrl":"https://doi.org/10.1246/cl.230377","url":null,"abstract":"Reaction of p-t-butylcalix[4]arene (BCA[4]) with α,ω-dibromoacetate (DBH) afforded a soluble cage compound, BCA[4]-DBH, in high yield. Single-crystal X-ray analysis revealed that BCA[4]-DBH contains a fixed cavity. Examination of host-guest chemistry showed that BCA[4]-DBH forms a 1 : 1 inclusion complex with phenol, with an association constant (K) of 34. Reaction of p-t-butylcalix[4]arene (BCA[4]) with α,ω-dibromoacetate (DBH) afforded a soluble cage compound, BCA[4]-DBH, in high yield. Single-crystal X-ray analysis revealed that BCA[4]-DBH contains a fixed cavity. Examination of host-guest chemistry showed that BCA[4]-DBH forms a 1 : 1 inclusion complex with phenol, with an association constant (K) of 34.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135805497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of hydrophobic attraction between polystyrene layer and silanated silica surface by atomic force microscopy 用原子力显微镜评价聚苯乙烯层与硅烷表面之间的疏水吸引力
4区 化学
Chemistry Letters Pub Date : 2023-10-13 DOI: 10.1246/cl.230363
Yuta Okuda, Koreyoshi Imamura, Naoyuki Ishida
{"title":"Evaluation of hydrophobic attraction between polystyrene layer and silanated silica surface by atomic force microscopy","authors":"Yuta Okuda, Koreyoshi Imamura, Naoyuki Ishida","doi":"10.1246/cl.230363","DOIUrl":"https://doi.org/10.1246/cl.230363","url":null,"abstract":"In this study, hydrophobic surfaces were prepared by depositing polystyrene (PS) on a glass substrate using three different methods to measure the interaction forces against a hydrophobized particle by atomic force microscopy. We found that smooth surfaces prepared by spin coating and living radical polymerization generated hydrophobic attraction, whereas uneven surfaces obtained by free radical polymerization produced only steric repulsion, even though they had sufficient hydrophobicity. Based on these results, the origin of the hydrophobic attraction was suggested. The interaction forces between hydrophobic surfaces prepared by depositing polystyrene on a glass substrate using three different methods and a hydrophobized particle were measured by atomic force microscopy. Surfaces prepared by spin coating and living radical polymerization generated hydrophobic attraction, whereas surfaces obtained by free radical polymerization produced no hydrophobic attraction, despite sufficient hydrophobicity. Based on these results, the origin of the hydrophobic attraction was suggested.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135805496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Theoretical Study on TiOOH Production over Au/TiO2 Catalyst — Support Dependency of Propylene Oxide Production— Au/TiO2催化剂制备TiOOH的理论研究——环氧丙烷生产的载体依赖性
4区 化学
Chemistry Letters Pub Date : 2023-10-07 DOI: 10.1246/cl.230367
Yasutaka Hamada, Yuki Mishima, Tomohisa Yonemori, Takashi Kawakami, Shusuke Yamanaka, Mitsutaka Okumura
{"title":"Theoretical Study on TiOOH Production over Au/TiO<sub>2</sub> Catalyst — Support Dependency of Propylene Oxide Production—","authors":"Yasutaka Hamada, Yuki Mishima, Tomohisa Yonemori, Takashi Kawakami, Shusuke Yamanaka, Mitsutaka Okumura","doi":"10.1246/cl.230367","DOIUrl":"https://doi.org/10.1246/cl.230367","url":null,"abstract":"Au supported anatase TiO2 enables the selective synthesis of propylene oxide from propylene using O2 and H2. However, this reaction does not proceed on Au supported rutile TiO2. The origin of this difference in the catalytic activity was examined using DFT calculations. DFT calculations revealed that the stability of the TiOOH active species was directly related to the product selectivity. Au supported anatase TiO2 enables the selective synthesis of propylene oxide from propylene using O2 and H2, while this reaction does not proceed on Au supported rutile TiO2. This difference of the catalytic activity was examined using DFT calculation. From the calculation results, it was found that the stability of TiOOH is directly related to the product selectivity.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135252067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selective Self-Assembly of Gold Nanocrystals using Complementary Leucine Zipper Peptides 利用互补亮氨酸拉链肽选择性自组装金纳米晶体
4区 化学
Chemistry Letters Pub Date : 2023-10-06 DOI: 10.1246/cl.230379
Issei Nakao, Kento Sumida, Mizuki Kitamatsu, Tetsuro Soejima
{"title":"Selective Self-Assembly of Gold Nanocrystals using Complementary Leucine Zipper Peptides","authors":"Issei Nakao, Kento Sumida, Mizuki Kitamatsu, Tetsuro Soejima","doi":"10.1246/cl.230379","DOIUrl":"https://doi.org/10.1246/cl.230379","url":null,"abstract":"A heterodimeric leucine zipper (Lz)–based peptide hybrid was utilized as a molecular adhesive to bound two types of gold nanocrystals with different shapes. An acidic leucine zipper (LzE) and a basic leucine zipper (LzK) peptides with terminal cysteine are respectively adsorbed to the surface of spherical gold nanoparticles (LzE–Au NPs) and gold nanocubes (LzK–Au NCs). Transmission electron microscope (TEM) observation and visible (Vis) absorption spectroscopy were revealed that LzE–Au NPs and LzK–Au NCs are selectively self-assembled in water. The dynamic self-assembly behavior is also confirmed by in-situ fluorescence resonance energy transfer between fluorescence dyes connected to LzE and LzK peptides and other control experiments. Complementary acidic leucine zipper (LzE) and basic leucine zipper (LzK) peptides were successfully adsorbed to the surface of spherical gold nanoparticles (Au NPs) and gold nanocubes (Au NCs), respectively. Selective self-assembly of LzE–Au NPs and LzK–Au NCs was occurred by hydrophobic interaction between leucine residues and electrostatic interaction between K (Lys) and E (Glu) amino acids.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135303452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Novel Characteristic of a Water-soluble UV-B Absorber, 2-Phenylbenzimidazole-5-sulfonic Acid: Suppression of Riboflavin-sensitized Singlet Oxygen Generation 水溶性UV-B吸收剂2-苯基苯并咪唑-5-磺酸的新特性:抑制核黄素敏化的单线态氧生成
4区 化学
Chemistry Letters Pub Date : 2023-10-05 DOI: 10.1246/cl.230330
Soichiro Hisanaga, Kazuyuki Miyazawa, Mikio Yagi, Azusa Kikuchi
{"title":"A Novel Characteristic of a Water-soluble UV-B Absorber, 2-Phenylbenzimidazole-5-sulfonic Acid: Suppression of Riboflavin-sensitized Singlet Oxygen Generation","authors":"Soichiro Hisanaga, Kazuyuki Miyazawa, Mikio Yagi, Azusa Kikuchi","doi":"10.1246/cl.230330","DOIUrl":"https://doi.org/10.1246/cl.230330","url":null,"abstract":"2-Phenylbenzimidazole-5-sulfonic acid (PBSA) is a water-soluble UV-B absorber used in cosmetic sunscreens. Riboflavin (RF) is a water-soluble vitamin B2. RF is an efficient singlet oxygen photosensitizer. The effects of PBSA on RF-photosensitized singlet oxygen generation have been studied through measurements of transient absorption and time-resolved near-IR phosphorescence in phosphate buffer (pH 7.4). PBSA suppresses the RF-photosensitized singlet oxygen generation. The observed suppression can be ascribed to the quenching of the lowest excited triplet state of RF by PBSA. 2-Phenylbenzimidazole-5-sulfonic acid (PBSA) is a water-soluble UV-B absorber used in cosmetic sunscreens. Riboflavin (RF) is a water-soluble vitamin B2. RF is an efficient singlet oxygen photosensitizer. The effects of PBSA on RF-photosensitized singlet oxygen generation have been studied through measurements of transient absorption and time-resolved near-IR phosphorescence in phosphate buffer (pH 7.4). PBSA suppresses the RF-photosensitized singlet oxygen generation.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135547607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Au/Nb2O5-Catalyzed N-Formylation of Amines Utilizing High Selectivity to Formate Intermediate in CO2 Hydrogenation Au/ nb2o5催化氨基n -甲酰化在CO2加氢反应中的高选择性
4区 化学
Chemistry Letters Pub Date : 2023-10-05 DOI: 10.1246/cl.230365
Mitsuyo Hidaka, Mingyue Lin, Hiroki Yamakawa, Akihiro Nakayama, Toru Murayama, Tetsuya Shimada, Shinsuke Takagi, Tamao Ishida
{"title":"Au/Nb<sub>2</sub>O<sub>5</sub>-Catalyzed <i>N</i>-Formylation of Amines Utilizing High Selectivity to Formate Intermediate in CO<sub>2</sub> Hydrogenation","authors":"Mitsuyo Hidaka, Mingyue Lin, Hiroki Yamakawa, Akihiro Nakayama, Toru Murayama, Tetsuya Shimada, Shinsuke Takagi, Tamao Ishida","doi":"10.1246/cl.230365","DOIUrl":"https://doi.org/10.1246/cl.230365","url":null,"abstract":"Deformed orthorhombic niobium oxide-supported Au nanoparticles (Au/Nb2O5-DO) exhibited high catalytic activity for N-formylation of amines using CO2 and H2 compared to other Au/Nb2O5 catalysts having different crystalline structures. Au/Nb2O5-DO also showed high selectivity to methyl formate in CO2 hydrogenation. Nb2O5-DO possessed greater amount of acid sites than Au/Nb2O5-T (orthorhombic) and Au/Nb2O5-A (amorphous), which would be beneficial for the formate activation and amine adsorption. Deformed orthorhombic niobium oxide-supported Au nanoparticles (Au/Nb2O5-DO) exhibited high catalytic activity for N-formylation of amines using CO2 and H2 compared to other Au/Nb2O5 catalysts having different crystalline structures. Au/Nb2O5-DO also showed high selectivity to formate in CO2 hydrogenation. Nb2O5-DO possessed greater amount of acid sites than Au/Nb2O5-T (orthorhombic) and Au/Nb2O5-A (amorphous), which would be beneficial for the formate activation and amine adsorption.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134947748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wrapping of Drug Assembly with Silica Layer and Confinement through Reversible Linkage with Copper(II) for Glutathione-Responsive Nanocapsule 谷胱甘肽响应纳米胶囊中硅层包裹药物组件和铜(II)可逆连接约束
4区 化学
Chemistry Letters Pub Date : 2023-10-05 DOI: 10.1246/cl.230322
Hideki Matsune, Ryoya Yoshida, Ren Ikemizu, Koichiro Shiomori, Tsuyoshi Yamamoto, Masahiro Kishida
{"title":"Wrapping of Drug Assembly with Silica Layer and Confinement through Reversible Linkage with Copper(II) for Glutathione-Responsive Nanocapsule","authors":"Hideki Matsune, Ryoya Yoshida, Ren Ikemizu, Koichiro Shiomori, Tsuyoshi Yamamoto, Masahiro Kishida","doi":"10.1246/cl.230322","DOIUrl":"https://doi.org/10.1246/cl.230322","url":null,"abstract":"Colloidal nano-assembly containing doxorubicin (DOX) is prepared for the first time by co-precipitation with gluconate. The spherical assembly is wrapped with a porous silica layer via sol-gel process, and confined in the formed cage by treatment with copper(II) ions (Cu2+). The reversible linking of DOX with Cu2+ is allowed to suppress the leakage of DOX, but the quick release is turned on in response to glutathione (GSH). The synthesized stimulus-responsive nanocapsules are characterized, and examined in the release test with/without GSH and applied to living HeLa cells. Spherical molecular assembly containig doxorubicin (DOX) is prepared by co-precipitatinon of doxorubicin hydrochloride and sodium gluconate (NaGlc), and then wrapped with porous silica layer via sol-gel process. The permeation of Cu2+ is allowed to suppress the leaking of the contents due to the blocking of the microchannels in the surronding layer, but release DOX immediately in response to glutathione (GSH).","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134947749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stabilization of DNA-encapsulating Droplets through Negative Charge at the Droplet Interface 通过液滴界面负电荷稳定dna包封液滴
4区 化学
Chemistry Letters Pub Date : 2023-10-05 DOI: 10.1246/cl.230294
Mayu Shono, Fumika Fujita, Kenichi Yoshikawa, Akihisa Shioi
{"title":"Stabilization of DNA-encapsulating Droplets through Negative Charge at the Droplet Interface","authors":"Mayu Shono, Fumika Fujita, Kenichi Yoshikawa, Akihisa Shioi","doi":"10.1246/cl.230294","DOIUrl":"https://doi.org/10.1246/cl.230294","url":null,"abstract":"It was found that stable cell-sized droplets entrapping DNA molecules are spontaneously generated through micro segregation. To clarify the mechanism, we performed measurements of the electrification by adapting a polymer solution with polyethylene glycol/dextran. We determined the Donnan potential for macroscopic phase segregation in both the presence and absence of DNA, together with the measurements of Zeta potentials of the microphase solution with number of small droplets. We confirmed that the droplets entrapping DNA are negatively charged. Stable cell-sized droplets entrapping DNA are spontaneously generated through micro segregation of polyethylene glycol/dextran solution. To clarify the mechanism, we determined the Donnan potential for macroscopic phase segregation in both with and without DNA, together with the Zeta potential measurements of the microphase solution with number of small droplets. We confirmed that the DNA-encapsulating droplets are negatively charged.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135547606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Machine Learning Accelerated Discovery of Subnanoparticles for Electrocatalytic Hydrogen Evolution
4区 化学
Chemistry Letters Pub Date : 2023-10-05 DOI: 10.1246/cl.230310
Quan Zou, Akiyoshi Kuzume, Masataka Yoshida, Takane Imaoka, Kimihisa Yamamoto
{"title":"Machine Learning Accelerated Discovery of Subnanoparticles for Electrocatalytic Hydrogen Evolution","authors":"Quan Zou, Akiyoshi Kuzume, Masataka Yoshida, Takane Imaoka, Kimihisa Yamamoto","doi":"10.1246/cl.230310","DOIUrl":"https://doi.org/10.1246/cl.230310","url":null,"abstract":"Metal and alloy subnanoparticles (SNPs) have been anticipated to be a class of promising catalysts because of their fundamental difference from nanoparticles (NPs). In general, the interaction among the surface and bulk atoms of SNPs is significant due to the higher degree of alloying in SNPs than that in NPs counterparts. This study compared the SNPs and NPs concerning their electrocatalytic activities of hydrogen evolution reaction (HER) to understand the essential difference between alloy SNPs and NPs by using machine learning. Phase segregation does not occur on multimetallic subnanoparticles (SNPs), but easily occurs on nanoparticles (NPs), which led to the homogeneously alloying of SNPs and unpredictable phase segregation of NPs. Because of this distinct behavior between SNPs and NPs, the HER activity of SNPs should be easier to predict than NPs. Therefore, machine learning was applied to accelerate the discovery of SNPs.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135547243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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