Cell medicine最新文献

{"title":"Comparison of Incubation Solutions Prior to the Purification of Porcine Islet Cells.","authors":"Takashi Kawai,&nbsp;Hirofumi Noguchi,&nbsp;Takashi Kuise,&nbsp;Atsuko Nakatsuka,&nbsp;Akihiro Katayama,&nbsp;Noriko Imagawa,&nbsp;Hitomi Usui Kataoka,&nbsp;Issei Saitoh,&nbsp;Yasufumi Noguchi,&nbsp;Masami Watanabe,&nbsp;Toshiyoshi Fujiwara","doi":"10.3727/215517913X674180","DOIUrl":"10.3727/215517913X674180","url":null,"abstract":"<p><p>For pancreatic islet transplantation, one of the most important steps of islet isolation is islet purification. The most common method of islet purification is density gradient centrifugation because there are differences in density between islets and acinar tissue. However, the density of islets/acinar tissue depends on several conditions, such as the incubation time before purification and the osmolality of the preincubation solution. In this study, we evaluated the impact of using two different preincubation solutions before purification. We used the University of Wisconsin (UW) solution and a new preservation solution (HN-1), which we recently developed. There were no significant differences between the two solutions in terms of the islet yield, rate of viability, and purity or stimulation index after purification. There were also no differences in the attainability and suitability of posttransplantation normoglycemia. Our study shows that the HN-1 solution is equivalent to the UW solution for preincubation before islet purification. </p>","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"6 1-2 1","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"2013-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X674180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69755965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organ Biology-New Development.","authors":"H. Noguchi","doi":"10.3727/215517913X674162","DOIUrl":"https://doi.org/10.3727/215517913X674162","url":null,"abstract":"On behalf of the Japan Society for Organ Preservation and Medical Biology (JSOPMB), I express my sincere appreciation to Professor Paul R. Sanberg (Department of Neurosurgery and Brain Repair, Morsani College of Medicine, University of South Florida, FL, USA), Executive Editor of Cell Medicine, for providing us such an excellent opportunity to publish the data that were presented at the annual meeting of the JSOPMB. I also thank Dr. David Eve, Associate Editor of Cell Medicine, for the editing of our articles in detail. I am very sure that the relationship between Cell Medicine and JSOPMB has enhanced the motivation of JSOPMB members as well as board members and will continue to do so in the future, while also encouraging young Japanese researchers to join this organization. \u0000 \u0000One of the extremely important missions of the annual meeting of the JSOPMB is to exchange new research outcomes and create new therapeutic concepts. JSOPMB always encourages and motivates young investigators. JSOPMB was started in 1974 for the study of organ preservation and developed widely in the 1990s with the participation of researchers in various fields of medicine, pharmacology, engineering, veterinary medicine, and basic science. Currently, JSOPMB has more than 400 members and is run under the direction of Dr. Takehide Asano, the president of the JSOPMB. \u0000 \u0000Excellent presentations conducted at the 39th annual meeting of the JSOPMB held November 16–17, 2012, in Fukushima, Japan, under the supervision of Dr. Mitsukazu Gotoh (Professor, Department of Regenerative Surgery, Fukushima Medical University, Fukushima, Japan), were selected and given an opportunity to be published in this special issue of Cell Medicine. Twelve of these presentations are herein published in this special JSOPMB issue. \u0000 \u0000There were five articles regarding pancreatic islet transplantation. Katayama et al. compared University of Wisconsin (UW) solution (gold standard preservation solution) with new preservation solution, HN-1, in pancreas preservation for islet isolation. Islet yield and islet graft function were significantly higher in the HN-1 group than the UW group. Kawai et al. evaluated the impact of UW and HN-1 solutions for preincubation before purification. The efficacy of the preincubation solutions and the quality of the isolated islets were similar. Kubota et al. studied the viability of Rho-associated protein kinase (ROCK) inhibitor Y-27632 in a culture system in vitro on isolated islets. Y-27632 inhibited cell apoptosis in a graft and was also indicated as effective in insulin secretion. Seita et al. created type 1 diabetes canine models that were suitable for the assessment of cell therapies, such as islet transplantation and bioartificial pancreas, with low-dose streptozotocin (STZ) injection and partial pancreatectomy. Yamashita et al. explored the potential of internationally transporting human islets from Alberta, Canada to Tokyo, Japan and obtaining viable dispersed islet ce","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"6 1-2 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X674162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69755853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioimaging of Transgenic Rats Established at Jichi Medical University: Applications in Transplantation Research.","authors":"T. Teratani, E. Kobayashi","doi":"10.3727/215517913X666549","DOIUrl":"https://doi.org/10.3727/215517913X666549","url":null,"abstract":"Research in the life sciences has been greatly advanced by the ability to directly visualize cells, tissues, and organs. Preclinical studies often involve many small and large animal experiments and, frequently, cell and organ transplantations. The rat is an excellent animal model for the development of transplantation and surgical techniques because of its small size and ability to breed in small spaces. Ten years ago, we established color-imaging transgenic rats and methods for the direct visualization of their tissues. Since then, our transgenic rats have been used throughout the various fields that are concerned with cell transplantation therapy. In this minireview, we summarize results from some of the groups that have used our transgenic rats at the bench level and in cell transplantation research.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"5 2-3 1","pages":"45-51"},"PeriodicalIF":0.0,"publicationDate":"2013-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X666549","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69755884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotype and Stability of Neural Differentiation of Androgenetic Murine ES Cell-Derived Neural Progenitor Cells.","authors":"W. Wolber, Ruhel Ahmad, S. Choi, S. Eckardt, K. McLaughlin, Jessica Schmitt, C. Geis, M. Heckmann, A. Sirén, A. Müller","doi":"10.3727/215517913X666468","DOIUrl":"https://doi.org/10.3727/215517913X666468","url":null,"abstract":"Uniparental zygotes with two paternal (androgenetic, AG) or two maternal genomes (gynogenetic, GG) cannot develop into viable offsprings but form blastocysts from which pluripotent embryonic stem (ES) cells can be derived. For most organs, it is unclear whether uniparental ES cells can give rise to stably expandable somatic stem cells that can repair injured tissues. Even if previous reports indicated that the capacity of AG ES cells to differentiate in vitro into pan-neural progenitor cells (pNPCs) and into cells expressing neural markers is similar to biparental [normal fertilized (N)] ES cells, their potential for functional neurogenesis is not known. Here we show that murine AG pNPCs give rise to neuron-like cells, which then generate sodium-driven action potentials while maintaining fidelity of imprinted gene expression. Neural engraftment after intracerebral transplantation was achieved only by late (22 days) AG and N pNPCs with in vitro low colony-forming cell (CFC) capacity. However, persisting CFC formation seen, in particular, in early (13 or 16 days) differentiation cultures of N and AG pNPCs correlated with a high incidence of trigerm layer teratomas. As AG ES cells display functional neurogenesis and in vivo stability similar to N ES cells, they represent a unique model system to study the roles of paternal and maternal genomes on neural development and on the development of imprinting-associated brain diseases.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"5 1 1","pages":"29-42"},"PeriodicalIF":0.0,"publicationDate":"2013-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X666468","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69756052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved Recovery of Hepatocytes Isolated From Warm Ischemic Rat Liver by Citrate Phosphate Dextrose (CPD)-Supplemented Euro-Collins Solution.","authors":"H. Hsu, N. Matsuno, N. Machida, S. Enosawa","doi":"10.3727/215517913X666521","DOIUrl":"https://doi.org/10.3727/215517913X666521","url":null,"abstract":"Demand for human primary hepatocytes is increasing, particularly for clinical trials of hepatocyte transplantation. However, due to the severe shortage of organ transplant donors, the source of cells for these endeavors is restricted to untransplantable livers, such as those from non-heart-beating donors and surgically resected liver tissues. To improve cell recovery from such sources after warm ischemia, we evaluated the efficacy of applying perfusion solutions, focusing on improvement of hepatocyte recovery. Warm ischemia was induced by clamping both portal vein and hepatic artery for 10 or 15 min in rats. The liver was perfused with either Euro-Collins (EC) or extracellular-type trehalose-containing Kyoto (ETK) solutions supplemented with an anticoagulant, either heparin or citrate phosphate dextrose solution (CPD), compared to Ca(2+), Mg(2+)-free Hanks solution. While the viability of recovered cells was 81.5 ± 4.2% and cell yield was 2.27 ± 0.53 × 10(8) in nonwarm ischemia controls (n = 11), these values were only 74.7 ± 2.9% and 0.38 ± 0.17 × 10(8), respectively, in the 10-min warm ischemia group, using the Hanks as the perfusion solution. Although the addition of heparin increased the live cell number only twofold (0.71 ± 0.40 × 10(8), n = 4), the best improvement was achieved by adding CPD to EC. This resulted in a recovery of 1.41 ± 0.50 × 10(8) in the 10-min ischemia group (n = 7) and 1.37 ± 0.28 × 10(8) in the 15-min group (n = 3). Macroscopic observation showed that blood had been completely flushed out by the solution, suggesting good restoration of the microcirculation in ischemic liver. Using ETK instead of EC resulted in a slight decrease in efficacy. These results demonstrate that CPD, as opposed to heparin, is effective in ensuring liver microcirculation and flushing out the blood and that EC is the best perfusion solution for obtaining hepatocytes from ischemic liver.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"5 2-3 1","pages":"97-101"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X666521","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69755763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Autologous Islet Transplantation.","authors":"M. Maruyama, T. Kenmochi, N. Akutsu, K. Otsuki, T. Ito, I. Matsumoto, T. Asano","doi":"10.3727/215517913X666558","DOIUrl":"https://doi.org/10.3727/215517913X666558","url":null,"abstract":"Autologous islet transplantation after total or semitotal pancreatectomy aims to preserve insulin secretory function and prevent the onset of diabetes. The major indication for pancreatectomy is chronic pancreatitis with severe abdominal pain, a benign pancreatic tumor, and trauma. The metabolic outcome of autologous islet transplantation is better than that of allogeneic transplantation and depends on the number of transplanted islets. Achieving islet isolation from a fibrous or damaged pancreas is one of the biggest challenges of autologous islet transplantation; a major complication is portal vein thrombosis after crude islet infusion. However, the incidence of portal vein thrombosis has decreased as islet preparation techniques have improved over time.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"5 2-3 1","pages":"59-62"},"PeriodicalIF":0.0,"publicationDate":"2013-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X666558","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69755898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culture Conditions for Mouse Pancreatic Stem Cells.","authors":"H. Noguchi, I. Saitoh, H. Kataoka, Masami Watanabe, Yasufumi Noguchi, T. Fujiwara","doi":"10.3727/215517913X666495","DOIUrl":"https://doi.org/10.3727/215517913X666495","url":null,"abstract":"Recently, mouse pancreatic stem cells have been isolated from adult mouse pancreata. However, these pancreatic stem cells could be maintained only under specific culture conditions with lot-limited fetal bovine serum (FBS). For the efficient isolation and maintenance of mouse pancreatic stem cells, it is important to identify culture conditions that can be used independent of the FBS lot. In this study, we evaluated the culture conditions required to maintain mouse pancreatic stem cells. The mouse pancreatic stem cells derived from the pancreas of a newborn mouse, HN#101, were cultured under the following conditions: 1) Dulbecco's modified Eagle's medium (DMEM) with 20% lot-limited FBS, in which mouse pancreatic stem cells could be cultured without changes in morphology and growth activity; 2) complete embryonic stem (ES) cell media; and 3) complete ES cell media on feeder layers of mitomycin C-treated STO cells, which were the same culture conditions used for mouse ES cells. Under culture conditions #1 and #3, the HN#101 cells continued to form a flat \"cobblestone\" monolayer and continued to divide actively beyond the population doubling level (PDL) 100 without growth inhibition, but this did not occur under culture condition #2. The gene expression profile and differentiated capacity of the HN#101 cells cultured for 2 months under culture condition #3 were similar to those of HN#101 cells at PDL 50. These data suggest that complete ES cell media on feeder layers could be useful for maintaining the undifferentiated state of pancreatic stem cells.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"5 2-3 1","pages":"63-8"},"PeriodicalIF":0.0,"publicationDate":"2013-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X666495","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69756129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Mesenchymal Stem Cell-Conditioned Medium to Activate Islets in Preservation Solution.","authors":"N. Kasahara, T. Teratani, J. Doi, Yuki Iijima, M. Maeda, S. Uemoto, Y. Fujimoto, N. Sata, Y. Yasuda, E. Kobayashi","doi":"10.3727/215517913X666477","DOIUrl":"https://doi.org/10.3727/215517913X666477","url":null,"abstract":"Pancreatic islet transplantation has received widespread attention as a promising treatment for type 1 diabetes. However, islets for transplantation are subject to damage from a number of sources, including ischemic injury during removal and delivery of the donor pancreas, enzymatic digestion during islet isolation, and reperfusion injury after transplantation in the recipient. Here we found that protein fractions secreted by mesenchymal stem cells (MSCs) were capable of activating preserved islets. A conditioned medium from the supernatant obtained by culturing adipose tissue MSCs (derived from wild-type Lewis rats) was prepared for 2 days in serum-free medium. Luc-Tg rat islets to which an organ preservation solution was added were then incubated at 4°C with fractions of various molecular weights prepared from the conditioned medium. Under the treatment with some of the fractions, by 4 days the relative luminescence intensities (representative of the ATP levels of the cold-preserved islets) had increased to over 150% of their initial values. Our novel system may be able to restore isolated islets to the condition they were in before transport, culture, and transplantation.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"5 2-3 1","pages":"75-81"},"PeriodicalIF":0.0,"publicationDate":"2013-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X666477","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69756087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation Efficiency of Mouse Pancreatic Stem Cells Is Age Dependent.","authors":"Takashi Kuise, H. Noguchi, I. Saitoh, H. Kataoka, Masami Watanabe, Yasufumi Noguchi, T. Fujiwara","doi":"10.3727/215517913X666503","DOIUrl":"https://doi.org/10.3727/215517913X666503","url":null,"abstract":"Mouse pancreatic stem cells have been isolated from mouse pancreata. This study evaluated the efficacy of isolating mouse pancreatic stem cells using mice of different ages. The pancreata of newborn mice, 8-week-old mice, and 24-week-old mice were harvested and digested by using collagenase. The \"duct-like\" cells in the digested pancreatic tissue were then inoculated into 96-well plates, cloned by limiting dilution, and cultured in DMEM with 20% FBS. Pancreatic stem cells were isolated from the pancreata of all newborn mice, while cells could only be isolated from 10% of the pancreata of 8-week-old mice and could not be isolated from the pancreata of any 24-week-old mice. These data suggest that young mice may have some pancreatic stem cells and that older mice may only have a few pancreatic stem cells. These data also indicate that it is extremely difficult to isolate pancreatic stem cells from older mice, suggesting that future research focus its efforts on finding methods of isolating pancreatic stem cells from adult mice.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"5 2-3 1","pages":"69-73"},"PeriodicalIF":0.0,"publicationDate":"2013-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X666503","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69756137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observation of Positively Charged Magnetic Nanoparticles Inside HepG2 Spheroids Using Electron Microscopy.","authors":"Y. Miyamoto, Yumie Koshidaka, H. Noguchi, Koichi Oishi, Hiroaki Saito, H. Yukawa, N. Kaji, T. Ikeya, Satoshi Suzuki, Hisashi Iwata, Y. Baba, K. Murase, S. Hayashi","doi":"10.3727/215517913X666530","DOIUrl":"https://doi.org/10.3727/215517913X666530","url":null,"abstract":"Magnetic resonance imaging (MRI) using magnetic nanoparticles has been used to diagnose vascular diseases as well as to monitor transplanted cells and tissues. In this study, we synthesized magnetic iron oxide nanoparticles (TMADM-03), electrically charged by the presence of a cationic end-group substitution of dextran, and observed these nanoparticles inside three-dimensional models of HepG2 spheroids, which mimic tissues. Patterned cell array glass disks were prepared to visualize the presence of TMADM-03 uptaken by HepG2 spheroids using transmission electron microscopy (TEM). The HepG2 cells (2 × 10(5) cells) were inoculated onto Cell-able™ 12-well plates. After 48 h of culture, the cells were incubated with 75 µg Fe/ml TMADM-03 in culture medium for 24 h. To investigate the cellular function of the HepG2 spheroids, the albumin secretion was evaluated by an ELISA. The albumin secretion after incubation for 24 h was reduced compared with the secretion prior to the addition of TMADM-03. TEM image samples were prepared in a planar direction or a vertical direction to the HepG2 spheroids on patterned cell array glass disks. The incorporation of TMADM-03 inside the HepG2 spheroids was confirmed. In addition, TMADM-03 could be observed in the deeper layers of the spheroids, and this was localized in the lysosomes. These data suggest that the novel magnetic iron oxide nanoparticles invade three-dimensional HepG2 spheroids.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":"5 2-3 1","pages":"89-96"},"PeriodicalIF":0.0,"publicationDate":"2013-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517913X666530","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69755778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}