Cell motility and the cytoskeleton最新文献_第5页

Three flagellar motilities in Chlamydomonas unrelated to flagellar beating. Video supplement. 衣藻的三种鞭毛运动与鞭毛跳动无关。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

K G Kozminski, P Forscher, J L Rosenbaum

引用次数: 0

The ankyrin-binding domain of CD44s is involved in regulating hyaluronic acid-mediated functions and prostate tumor cell transformation. CD44s的锚蛋白结合域参与调节透明质酸介导的功能和前列腺肿瘤细胞转化。

Cell motility and the cytoskeleton Pub Date : 1998-01-01 DOI: 10.1002/(SICI)1097-0169(1998)39:3<209::AID-CM4>3.0.CO;2-#

D Zhu, L Y Bourguignon

{"title":"The ankyrin-binding domain of CD44s is involved in regulating hyaluronic acid-mediated functions and prostate tumor cell transformation.","authors":"D Zhu, L Y Bourguignon","doi":"10.1002/(SICI)1097-0169(1998)39:3<209::AID-CM4>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1998)39:3<209::AID-CM4>3.0.CO;2-#","url":null,"abstract":"CD44 isoforms, such as CD44s (the standard form), contain at least one ankyrin-binding site within the 70-amino acid (aa) cytoplasmic domain and several hyaluronic acid (HA)-binding sites within the extracellular domain. To study the role of CD44s-ankyrin interaction in regulating human prostate tumor cells, we have constructed several CD44s cytoplasmic deletion mutants that lack the ankyrin-binding site(s). These truncated cDNAs were stably transfected into CD44-negative human prostate tumor cells (LNCaP). Our results indicate that a critical region of 15-amino acids (aa) between aa 304 and aa 318 of CD44s is required for ankyrin binding. Biochemical analyses, using competition binding assays with a synthetic peptide containing the 15 aa between aa 304 and aa 318 (NSGNGAVEDRKPSGL), further support the conclusion that this region contains the ankyrin-binding domain of CD44s. Deletion of this 15-aa ankyrin-binding sequence from CD44s results in a drastic reduction of HA-mediated binding/cell adhesion, Src p60 kinase(s) interaction and anchorage-independent growth in soft agar. These findings suggest that the binding of cytoskeletal proteins, such as ankyrin, to the cytoplasmic domain of CD44s plays a pivotal role in regulating HA-mediated functions as well as Src kinase activity and prostate tumor cell transformation.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"39 3","pages":"209-22"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0169(1998)39:3<209::AID-CM4>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20442489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Interactions of Listeria monocytogenes with infected host cells. Video supplement. 单核增生李斯特菌与受感染宿主细胞的相互作用。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

J M Sanger, F G Dold, D Nanavati, J W Sanger

引用次数: 0

Measurement of traction forces in cells locomoting along a substratum. Video supplement. 测量细胞沿基质运动时的牵引力。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

T Oliver, M Dembo, K Jacobson

引用次数: 0

Occurrence of fibers and their association with talin in the cleavage furrow of PtK2 cells. Video supplement. PtK2细胞卵裂沟中纤维的出现及其与talin的关联。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

J M Sanger, J S Dome, R S Hock, B Mittal, J W Sanger

引用次数: 0

Computer modelling of the ciliary axoneme. Video supplement. 睫状体轴突的计算机模拟。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

M Holwill, G Foster, E Guevara, T Hamasaki, P Satir

引用次数: 0

Translocation of myelin basic protein mRNA in oligodendrocytes requires microtubules and kinesin. 髓鞘碱性蛋白mRNA在少突胶质细胞中的易位需要微管和运动蛋白。

Cell motility and the cytoskeleton Pub Date : 1997-01-01 DOI: 10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2

J. Carson, K. Worboys, K. Ainger, E. Barbarese

{"title":"Translocation of myelin basic protein mRNA in oligodendrocytes requires microtubules and kinesin.","authors":"J. Carson, K. Worboys, K. Ainger, E. Barbarese","doi":"10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2","url":null,"abstract":"Myelin basic protein (MBP) mRNA is localized to the myelin membranes of oligodendrocytes. When exogenous MBP mRNA is microinjected into oligodendrocytes in culture, it is transported along the processes and localized to the myelin compartment in a multistep intracellular RNA trafficking pathway. In the work described here, oligodendrocytes were treated with agents that affect the cytoskeleton including: nocodazole, to disrupt microtubules; taxol, to stabilize microtubules; cytochalasin, to disrupt microfilaments; and kinesin anti-sense oligonucleotide, to suppress kinesin expression. Digoxigenin-labeled MBP mRNA was microinjected into the treated cells and the extent of translocation of the microinjected RNA was determined by confocal microscopy. Nocodazole, taxol, and kinesin anti-sense oligonucleotide inhibited translocation of microinjected MBP mRNA, while cytochalasin B and kinesin sense oligonucleotide did not. These results indicate that translocation of MBP mRNA in oligodendrocytes requires intact microtubules and kinesin but does not require intact microfilaments. The results are discussed in relation to the current multistep model for intracellular RNA trafficking in oligodendrocytes.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"1 4","pages":"318-28"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50667408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 111

Translocation of myelin basic protein mRNA in oligodendrocytes requires microtubules and kinesin. 髓鞘碱性蛋白mRNA在少突胶质细胞中的易位需要微管和运动蛋白。

Cell motility and the cytoskeleton Pub Date : 1997-01-01 DOI: 10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2-#

J H Carson, K Worboys, K Ainger, E Barbarese

{"title":"Translocation of myelin basic protein mRNA in oligodendrocytes requires microtubules and kinesin.","authors":"J H Carson, K Worboys, K Ainger, E Barbarese","doi":"10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2-#","url":null,"abstract":"Myelin basic protein (MBP) mRNA is localized to the myelin membranes of oligodendrocytes. When exogenous MBP mRNA is microinjected into oligodendrocytes in culture, it is transported along the processes and localized to the myelin compartment in a multistep intracellular RNA trafficking pathway. In the work described here, oligodendrocytes were treated with agents that affect the cytoskeleton including: nocodazole, to disrupt microtubules; taxol, to stabilize microtubules; cytochalasin, to disrupt microfilaments; and kinesin anti-sense oligonucleotide, to suppress kinesin expression. Digoxigenin-labeled MBP mRNA was microinjected into the treated cells and the extent of translocation of the microinjected RNA was determined by confocal microscopy. Nocodazole, taxol, and kinesin anti-sense oligonucleotide inhibited translocation of microinjected MBP mRNA, while cytochalasin B and kinesin sense oligonucleotide did not. These results indicate that translocation of MBP mRNA in oligodendrocytes requires intact microtubules and kinesin but does not require intact microfilaments. The results are discussed in relation to the current multistep model for intracellular RNA trafficking in oligodendrocytes.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"38 4","pages":"318-28"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20344685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 126

Cell cycle-dependent regulation of cellular ATP concentration, and depolymerization of the interphase microtubular network induced by elevated cellular ATP concentration in whole fibroblasts. 细胞周期依赖性的细胞ATP浓度调控，以及全成纤维细胞ATP浓度升高诱导的间期微管网络解聚。

Cell motility and the cytoskeleton Pub Date : 1996-01-01 DOI: 10.1002/(SICI)1097-0169(1996)35:2<94::AID-CM2>3.0.CO;2-I

M Marcussen, P J Larsen

{"title":"Cell cycle-dependent regulation of cellular ATP concentration, and depolymerization of the interphase microtubular network induced by elevated cellular ATP concentration in whole fibroblasts.","authors":"M Marcussen, P J Larsen","doi":"10.1002/(SICI)1097-0169(1996)35:2<94::AID-CM2>3.0.CO;2-I","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1996)35:2<94::AID-CM2>3.0.CO;2-I","url":null,"abstract":"In the present work, evidence is presented indicating that an increased cellular ATP concentration during mitosis may, in conjunction with other factors [Verde et al., 1990: Nature 343:233-238; Andersen et al., 1994: J Cell Biol. 127:1289-1299], induce depolymerization of the interphase microtubular network in cultured fibroblasts. It is shown here that the cellular ATP concentration varies through the cell cycle, reaching a peak at G2M- and minimum at late G1/early S-phase. Furthermore, we have found, using indirect immunofluorescent staining with an antitubulin antibody, that depolymerization of the interphase microtubular network may be induced by increasing the intracellular ATP concentration in cultured fibroblasts from 2.2 mM to 4.1 mM. This may be obtained through addition of adenosine and P1 to the growth medium. Our results indicate that this effect of adenosine and Pi is not mediated via adenosine receptors, but through an elevated cellular ATP concentration. ATP is suggested to act through a concentration-dependent effect on the exchangeable GTP site on tubulin, and not through the action of protein kinases or microtubule-associated proteins.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"35 2","pages":"94-9"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0169(1996)35:2<94::AID-CM2>3.0.CO;2-I","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19859254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Critical centrifugal forces induce adhesion rupture or structural reorganization in cultured cells. 临界离心力诱导培养细胞粘附破裂或结构重组。

Cell motility and the cytoskeleton Pub Date : 1996-01-01 DOI: 10.1002/(SICI)1097-0169(1996)33:4<276::AID-CM4>3.0.CO;2-7

O Thoumine, A Ott, D Louvard

{"title":"Critical centrifugal forces induce adhesion rupture or structural reorganization in cultured cells.","authors":"O Thoumine, A Ott, D Louvard","doi":"10.1002/(SICI)1097-0169(1996)33:4<276::AID-CM4>3.0.CO;2-7","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1996)33:4<276::AID-CM4>3.0.CO;2-7","url":null,"abstract":"Cultured epithelial cells were exposed to accelerations ranging from 9,000 to 70,000g for time periods of 5, 15, or 60 min, by centrifugation in a direction tangential to their plastic substrate. Three regimes describe the cellular response: (1) Cell morphology and density remain unaltered at forces below a threshold of about 10(-7) N; (2) Between this critical force and a second threshold of about 1.5 10(-7)N, the number of adherent cells decreases exponentially with time and acceleration, with no alteration of cell morphology. This behavior can be modeled by a constant probability of detaching and by an exponential distribution of cell-to-substrate adhesive forces; (3) Past the second threshold, cells that are still adherent exhibit elongated morphologies, the degree of elongation increasing linearly with the force. The fact that cells lose their vinculin-rich focal contacts past the first threshold and that cells cultured on gelatin-coated plastic show an increased resistance to detachment suggests a rupture of cell-to-substrate adhesions upon centrifugation. Immunofluorescent labeling of cells for actin and tubulin shows a reorganization of the cytoskeleton upon centrifugation, and treatment of cells with the drugs cytochalasin D and nocodazole demonstrates that cytoskeletal elements are actively involved in the structural deformation of cells past the second acceleration threshold, microtubules and microfilaments paying antagonistic roles.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"33 4","pages":"276-87"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0169(1996)33:4<276::AID-CM4>3.0.CO;2-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19772646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 61