Cell motility and the cytoskeleton最新文献_第5页

Three flagellar motilities in Chlamydomonas unrelated to flagellar beating. Video supplement. 衣藻的三种鞭毛运动与鞭毛跳动无关。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

K G Kozminski, P Forscher, J L Rosenbaum

引用次数: 0

The ankyrin-binding domain of CD44s is involved in regulating hyaluronic acid-mediated functions and prostate tumor cell transformation. CD44s的锚蛋白结合域参与调节透明质酸介导的功能和前列腺肿瘤细胞转化。

Cell motility and the cytoskeleton Pub Date : 1998-01-01 DOI: 10.1002/(SICI)1097-0169(1998)39:3<209::AID-CM4>3.0.CO;2-#

D Zhu, L Y Bourguignon

{"title":"The ankyrin-binding domain of CD44s is involved in regulating hyaluronic acid-mediated functions and prostate tumor cell transformation.","authors":"D Zhu, L Y Bourguignon","doi":"10.1002/(SICI)1097-0169(1998)39:3<209::AID-CM4>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1998)39:3<209::AID-CM4>3.0.CO;2-#","url":null,"abstract":"CD44 isoforms, such as CD44s (the standard form), contain at least one ankyrin-binding site within the 70-amino acid (aa) cytoplasmic domain and several hyaluronic acid (HA)-binding sites within the extracellular domain. To study the role of CD44s-ankyrin interaction in regulating human prostate tumor cells, we have constructed several CD44s cytoplasmic deletion mutants that lack the ankyrin-binding site(s). These truncated cDNAs were stably transfected into CD44-negative human prostate tumor cells (LNCaP). Our results indicate that a critical region of 15-amino acids (aa) between aa 304 and aa 318 of CD44s is required for ankyrin binding. Biochemical analyses, using competition binding assays with a synthetic peptide containing the 15 aa between aa 304 and aa 318 (NSGNGAVEDRKPSGL), further support the conclusion that this region contains the ankyrin-binding domain of CD44s. Deletion of this 15-aa ankyrin-binding sequence from CD44s results in a drastic reduction of HA-mediated binding/cell adhesion, Src p60 kinase(s) interaction and anchorage-independent growth in soft agar. These findings suggest that the binding of cytoskeletal proteins, such as ankyrin, to the cytoplasmic domain of CD44s plays a pivotal role in regulating HA-mediated functions as well as Src kinase activity and prostate tumor cell transformation.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"39 3","pages":"209-22"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0169(1998)39:3<209::AID-CM4>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20442489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Interactions of Listeria monocytogenes with infected host cells. Video supplement. 单核增生李斯特菌与受感染宿主细胞的相互作用。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

J M Sanger, F G Dold, D Nanavati, J W Sanger

引用次数: 0

Measurement of traction forces in cells locomoting along a substratum. Video supplement. 测量细胞沿基质运动时的牵引力。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

T Oliver, M Dembo, K Jacobson

引用次数: 0

Occurrence of fibers and their association with talin in the cleavage furrow of PtK2 cells. Video supplement. PtK2细胞卵裂沟中纤维的出现及其与talin的关联。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

J M Sanger, J S Dome, R S Hock, B Mittal, J W Sanger

引用次数: 0

Computer modelling of the ciliary axoneme. Video supplement. 睫状体轴突的计算机模拟。视频的补充。

Cell motility and the cytoskeleton Pub Date : 1998-01-01

M Holwill, G Foster, E Guevara, T Hamasaki, P Satir

引用次数: 0

Translocation of myelin basic protein mRNA in oligodendrocytes requires microtubules and kinesin. 髓鞘碱性蛋白mRNA在少突胶质细胞中的易位需要微管和运动蛋白。

Cell motility and the cytoskeleton Pub Date : 1997-01-01 DOI: 10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2

J. Carson, K. Worboys, K. Ainger, E. Barbarese

{"title":"Translocation of myelin basic protein mRNA in oligodendrocytes requires microtubules and kinesin.","authors":"J. Carson, K. Worboys, K. Ainger, E. Barbarese","doi":"10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2","url":null,"abstract":"Myelin basic protein (MBP) mRNA is localized to the myelin membranes of oligodendrocytes. When exogenous MBP mRNA is microinjected into oligodendrocytes in culture, it is transported along the processes and localized to the myelin compartment in a multistep intracellular RNA trafficking pathway. In the work described here, oligodendrocytes were treated with agents that affect the cytoskeleton including: nocodazole, to disrupt microtubules; taxol, to stabilize microtubules; cytochalasin, to disrupt microfilaments; and kinesin anti-sense oligonucleotide, to suppress kinesin expression. Digoxigenin-labeled MBP mRNA was microinjected into the treated cells and the extent of translocation of the microinjected RNA was determined by confocal microscopy. Nocodazole, taxol, and kinesin anti-sense oligonucleotide inhibited translocation of microinjected MBP mRNA, while cytochalasin B and kinesin sense oligonucleotide did not. These results indicate that translocation of MBP mRNA in oligodendrocytes requires intact microtubules and kinesin but does not require intact microfilaments. The results are discussed in relation to the current multistep model for intracellular RNA trafficking in oligodendrocytes.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"1 4","pages":"318-28"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50667408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 111

Translocation of myelin basic protein mRNA in oligodendrocytes requires microtubules and kinesin. 髓鞘碱性蛋白mRNA在少突胶质细胞中的易位需要微管和运动蛋白。

Cell motility and the cytoskeleton Pub Date : 1997-01-01 DOI: 10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2-#

J H Carson, K Worboys, K Ainger, E Barbarese

{"title":"Translocation of myelin basic protein mRNA in oligodendrocytes requires microtubules and kinesin.","authors":"J H Carson, K Worboys, K Ainger, E Barbarese","doi":"10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2-#","url":null,"abstract":"Myelin basic protein (MBP) mRNA is localized to the myelin membranes of oligodendrocytes. When exogenous MBP mRNA is microinjected into oligodendrocytes in culture, it is transported along the processes and localized to the myelin compartment in a multistep intracellular RNA trafficking pathway. In the work described here, oligodendrocytes were treated with agents that affect the cytoskeleton including: nocodazole, to disrupt microtubules; taxol, to stabilize microtubules; cytochalasin, to disrupt microfilaments; and kinesin anti-sense oligonucleotide, to suppress kinesin expression. Digoxigenin-labeled MBP mRNA was microinjected into the treated cells and the extent of translocation of the microinjected RNA was determined by confocal microscopy. Nocodazole, taxol, and kinesin anti-sense oligonucleotide inhibited translocation of microinjected MBP mRNA, while cytochalasin B and kinesin sense oligonucleotide did not. These results indicate that translocation of MBP mRNA in oligodendrocytes requires intact microtubules and kinesin but does not require intact microfilaments. The results are discussed in relation to the current multistep model for intracellular RNA trafficking in oligodendrocytes.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"38 4","pages":"318-28"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20344685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 126

Cell cycle-dependent regulation of cellular ATP concentration, and depolymerization of the interphase microtubular network induced by elevated cellular ATP concentration in whole fibroblasts. 细胞周期依赖性的细胞ATP浓度调控，以及全成纤维细胞ATP浓度升高诱导的间期微管网络解聚。

Cell motility and the cytoskeleton Pub Date : 1996-01-01 DOI: 10.1002/(SICI)1097-0169(1996)35:2<94::AID-CM2>3.0.CO;2-I

M Marcussen, P J Larsen

{"title":"Cell cycle-dependent regulation of cellular ATP concentration, and depolymerization of the interphase microtubular network induced by elevated cellular ATP concentration in whole fibroblasts.","authors":"M Marcussen, P J Larsen","doi":"10.1002/(SICI)1097-0169(1996)35:2<94::AID-CM2>3.0.CO;2-I","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1996)35:2<94::AID-CM2>3.0.CO;2-I","url":null,"abstract":"In the present work, evidence is presented indicating that an increased cellular ATP concentration during mitosis may, in conjunction with other factors [Verde et al., 1990: Nature 343:233-238; Andersen et al., 1994: J Cell Biol. 127:1289-1299], induce depolymerization of the interphase microtubular network in cultured fibroblasts. It is shown here that the cellular ATP concentration varies through the cell cycle, reaching a peak at G2M- and minimum at late G1/early S-phase. Furthermore, we have found, using indirect immunofluorescent staining with an antitubulin antibody, that depolymerization of the interphase microtubular network may be induced by increasing the intracellular ATP concentration in cultured fibroblasts from 2.2 mM to 4.1 mM. This may be obtained through addition of adenosine and P1 to the growth medium. Our results indicate that this effect of adenosine and Pi is not mediated via adenosine receptors, but through an elevated cellular ATP concentration. ATP is suggested to act through a concentration-dependent effect on the exchangeable GTP site on tubulin, and not through the action of protein kinases or microtubule-associated proteins.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"35 2","pages":"94-9"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0169(1996)35:2<94::AID-CM2>3.0.CO;2-I","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19859254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

In vivo dynamics of myosin II in Dictyostelium by fluorescent analogue cytochemistry. 用荧光模拟细胞化学方法研究盘形骨菌中肌球蛋白II的体内动态。

Cell motility and the cytoskeleton Pub Date : 1996-01-01 DOI: 10.1002/(SICI)1097-0169(1996)35:3<254::AID-CM7>3.0.CO;2-8

Q Chu, Y Fukui

{"title":"In vivo dynamics of myosin II in Dictyostelium by fluorescent analogue cytochemistry.","authors":"Q Chu, Y Fukui","doi":"10.1002/(SICI)1097-0169(1996)35:3<254::AID-CM7>3.0.CO;2-8","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0169(1996)35:3<254::AID-CM7>3.0.CO;2-8","url":null,"abstract":"We used fluorescent analogue cytochemistry to study in vivo dynamics of myosin II in Dictyostelium discoideum. We labeled myosin with biotin or tetramethyl-rhodamine iodoacetamide (IATR). The labeled myosin shows normal activities as reversible filament assembly and Ca2+ and actin-activatable Mg(2+)-ATPase. We used the biotin-myosin as a probe examining the effects of microinjection on the amoebae and the ability to associate with endogenous actin cytoskeleton. The biotin-myosin incorporates into certain actin populations and localizes to the cortex with the highest accumulation in the posterior end of polarized amoebae. The dynamics in live amoebae were probed by TR-myosin. We monitored the dynamics for a long period to determined the dynamic reorganization corresponding specific cellular behaviors. The TR-myosin converges into a discrete actin- and myosin-rich structure located at the posterior end (\"myosin-organizing center\"). The rod-shaped TR-myosin exhibits linear orderly arrays emanating from the organizing center which extend about two-thirds of the cell length. The myosin arrays show a dynamic reorganization when the amoebae move. To examine if the observed myosin dynamics are related to filamentous (F-) actin, we disrupted the F-actin by cytochalasin D. The ratioed image of TR-myosin (vs. FITC-dextran) demonstrates that myosin in these cells accumulates in the cortex but does not form the organizing center. Overall, the results suggest that the filamentous myosin organizes into orderly arrays in the live cytoplasm and its translocation occurs by means of F-actin cables, converging into the organizing center.","PeriodicalId":9675,"journal":{"name":"Cell motility and the cytoskeleton","volume":"35 3","pages":"254-68"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0169(1996)35:3<254::AID-CM7>3.0.CO;2-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19878907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11