Cell regulation最新文献_第3页

Distinct determinants on collagen support alpha 2 beta 1 integrin-mediated platelet adhesion and platelet activation. 胶原蛋白的不同决定因素支持α 2 β 1整合素介导的血小板粘附和血小板活化。

Cell regulation Pub Date : 1991-11-01 DOI: 10.1091/mbc.2.11.905

S A Santoro, J J Walsh, W D Staatz, K J Baranski

{"title":"Distinct determinants on collagen support alpha 2 beta 1 integrin-mediated platelet adhesion and platelet activation.","authors":"S A Santoro, J J Walsh, W D Staatz, K J Baranski","doi":"10.1091/mbc.2.11.905","DOIUrl":"https://doi.org/10.1091/mbc.2.11.905","url":null,"abstract":"Recent studies have revealed that the sequence of amino acids asp-gly-glu-ala represents an essential determinant of the site within the alpha 1(I)-CB3 fragment of collagen recognized by the alpha 2 beta 1 integrin cell surface collagen receptor (Staatz et al., 1991). Studies employing chemical modifications of collagen amino acid side chains confirm both the essential nature of the acidic side chains of aspartic acid and glutamic acid residues and the nonessentiality of lysine epsilon-amino groups in supporting adhesion mediated by the alpha 2 beta 1 integrin. The approach also indicates the presence of a distinct determinant on collagen separate from the alpha 2 beta 1 recognition site that contains essential lysine side chains and that is necessary for subsequent interactions with the platelet surface that give rise to collagen-induced platelet activation and secretion. The two-step, two-site model for cellular signaling involving both an integrin and a signal-transducing coreceptor suggested by these data may be common to other integrin-mediated processes.","PeriodicalId":9671,"journal":{"name":"Cell regulation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1091/mbc.2.11.905","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12970790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 100

Laminin-binding integrin alpha 7 beta 1: functional characterization and expression in normal and malignant melanocytes. 层粘连蛋白结合整合素α 7 β 1:正常和恶性黑色素细胞的功能表征和表达。

Cell regulation Pub Date : 1991-10-01 DOI: 10.1091/mbc.2.10.805

R H Kramer, M P Vu, Y F Cheng, D M Ramos, R Timpl, N Waleh

{"title":"Laminin-binding integrin alpha 7 beta 1: functional characterization and expression in normal and malignant melanocytes.","authors":"R H Kramer, M P Vu, Y F Cheng, D M Ramos, R Timpl, N Waleh","doi":"10.1091/mbc.2.10.805","DOIUrl":"https://doi.org/10.1091/mbc.2.10.805","url":null,"abstract":"A novel integrin, alpha 7 beta 1, that specifically binds with high affinity to laminin has been identified on melanoma cells. This complex was purified from both human and murine melanoma cells by laminin-affinity chromatography, and the alpha 7 subunit was recovered after gel electrophoresis. N-terminal amino acid sequence analysis of the alpha 7 subunit from both human and mouse cells verifies that this integrin is distinct from other alpha chains in the beta 1 family, although strikingly similar to the alpha 6 subunit. By using specific proteolytically derived fragments of laminin, it was determined that the alpha 7 beta 1 complex binds selectively to the E8 region, which represents part of the long arm of laminin. In contrast, the receptor failed to bind to the P1 fragment, which contains the intersection of the short arms of laminin. Although the alpha 7 beta 1 complex was commonly expressed in melanoma cells, this integrin was not detected in normal melanocytes, suggesting that alpha 7 expression may be associated with malignant transformation. These results establish the existence of a novel integrin that binds to the E8 domain of laminin and appears to mediate cell adhesion to this ligand.","PeriodicalId":9671,"journal":{"name":"Cell regulation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1091/mbc.2.10.805","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13000068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 109

Activation of phospholipase D: a signaling system set in motion by perturbation of the T lymphocyte antigen receptor/CD3 complex. 磷脂酶D的激活:一个由T淋巴细胞抗原受体/CD3复合物的扰动而启动的信号系统。

Cell regulation Pub Date : 1991-10-01 DOI: 10.1091/mbc.2.10.841

S J Stewart, G R Cunningham, J A Strupp, F S House, L L Kelley, G S Henderson, J H Exton, S B Bocckino

{"title":"Activation of phospholipase D: a signaling system set in motion by perturbation of the T lymphocyte antigen receptor/CD3 complex.","authors":"S J Stewart, G R Cunningham, J A Strupp, F S House, L L Kelley, G S Henderson, J H Exton, S B Bocckino","doi":"10.1091/mbc.2.10.841","DOIUrl":"https://doi.org/10.1091/mbc.2.10.841","url":null,"abstract":"A number of cellular signaling systems are called into play by interaction of the T lymphocyte antigen receptor/CD3 complex with its cognate antigen. Well-described signaling systems include phosphoinositide turnover, tyrosine phosphorylation, protein kinase C activation, and increased cytosolic calcium. We have explored the possibility that another recently described signaling system, activation of phospholipase D, may be operative. Data presented here demonstrate that stimulation of Jurkat T cells with anti-CD3 antibodies or phorbol esters resulted in activation of phospholipase D, as measured by production of phosphatidylethanol and phosphatidic acid. The combination of anti-CD3 antibody plus phorbol ester led to a greater than additive production of phosphatidylethanol and to the additive production of phosphatidic acid (in the absence of ethanol). Phorbol esters as a second stimulus with anti-CD3 antibody led to a additive increase in cellular diacylglycerol content but provided no increased production of inositol phosphates, suggesting that diacylglycerol production in these cells results from hydrolysis of noninositol containing lipids as well as from phosphinositides. Exogenous addition of phosphatidic acid led to increases in cytosolic calcium that, depending on the concentration used, resulted from release of an intracellular store of calcium and influx of extracellular calcium. Changes in cytosolic calcium occurred in the absence of inositol phosphates production. These studies establish a role for increased phospholipase D activity in T lymphocyte activation.","PeriodicalId":9671,"journal":{"name":"Cell regulation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1091/mbc.2.10.841","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13000446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

The yeast SRM1 protein and human RCC1 protein share analogous functions. 酵母的SRM1蛋白和人的RCC1蛋白具有相似的功能。

Cell regulation Pub Date : 1991-10-01 DOI: 10.1091/mbc.2.10.781

K L Clark, M Ohtsubo, T Nishimoto, M Goebl, G F Sprague

{"title":"The yeast SRM1 protein and human RCC1 protein share analogous functions.","authors":"K L Clark, M Ohtsubo, T Nishimoto, M Goebl, G F Sprague","doi":"10.1091/mbc.2.10.781","DOIUrl":"https://doi.org/10.1091/mbc.2.10.781","url":null,"abstract":"The Saccharomyces cerevisiae protein SRM1 and the mammalian protein RCC1 have amino acid sequence similarity throughout their lengths. SRM1 was defined by a recessive mutation in yeast that both activates the signal transduction pathway required for mating and leads to arrest in the G1 phase of the cell cycle. RCC1 was defined by a recessive mutation in hamster cells that causes premature chromosome condensation and other characteristics of entry into mitosis. Despite the seemingly different roles implied by these phenotypes, we suggest that RCC1 and SRM1 proteins have similar functions. In particular, we find that RCC1 can complement the temperature-sensitive growth phenotype of two independent srm1 mutations and also complements, at least partially, phenotypes associated with activation of the pheromone response pathway, such as transcription induction of FUS1. However, RCC1 fails to complement an srm1 null allele. Further characterization of the srm1 mutant phenotype reveals a defect in plasmid and chromosome stability, suggesting that the mutants have a defect in DNA replication, mitosis, or their coordination. Finally, like RCC1, SRM1 is a nuclear protein. Together, these data imply that SRM1 and RCC1 have a common role in their respective organisms.","PeriodicalId":9671,"journal":{"name":"Cell regulation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1091/mbc.2.10.781","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12832100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Complementation between urokinase-producing and receptor-producing cells in extracellular matrix degradation. 细胞外基质降解中尿激酶生成细胞和受体生成细胞之间的互补。

Cell regulation Pub Date : 1991-10-01 DOI: 10.1091/mbc.2.10.793

P H Quax, N Pedersen, M T Masucci, E J Weening-Verhoeff, K Danø, J H Verheijen, F Blasi

{"title":"Complementation between urokinase-producing and receptor-producing cells in extracellular matrix degradation.","authors":"P H Quax, N Pedersen, M T Masucci, E J Weening-Verhoeff, K Danø, J H Verheijen, F Blasi","doi":"10.1091/mbc.2.10.793","DOIUrl":"https://doi.org/10.1091/mbc.2.10.793","url":null,"abstract":"The respective roles of urokinase plasminogen activator (u-PA) and the u-PA receptor in extracellular matrix degradation was investigated. Human pro-u-PA and the human u-PA receptor were expressed independently by two different mouse LB6 cell lines. The matrix degradation capacity of these cell lines individually or in coculture was studied. Although pro-u-PA-producing cells alone degrade the matrix in the presence of plasminogen, u-PA-receptor producing cells do not. Cocultivation of a small fraction of pro-u-PA-producing cells with the receptor-producing cells increases the rate of matrix degradation at least threefold. By immunoprecipitation it was shown that cocultivation of the two cell lines increases the conversion of the inactive pro-u-PA to the active two chain u-PA. The enhancement of matrix degradation and of pro-u-PA activation requires actual binding of pro-u-PA to its receptor because it is inhibited by u-PA-receptor antagonists. The u-PA receptor must be cell associated, as binding of pro-u-PA to a receptor solubilized from the cell surface with phosphatidyl-inositol specific phospholipase C did not enhance the activation of pro-u-PA in the presence of plasminogen. The finding that activity of u-PA is enhanced when it is bound to its receptor, even when the receptor is produced by a different cell, might have important implications for the mechanisms of u-PA-induced extracellular proteolysis in vivo.","PeriodicalId":9671,"journal":{"name":"Cell regulation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1091/mbc.2.10.793","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12832101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 81

Recombinant bovine neurokinin-2 receptor stably expressed in Chinese hamster ovary cells couples to multiple signal transduction pathways. 重组牛神经激肽-2受体在中国仓鼠卵巢细胞中稳定表达，偶联多种信号转导途径。

Cell regulation Pub Date : 1991-10-01 DOI: 10.1091/mbc.2.10.767

H R Eistetter, D J Church, A Mills, P P Godfrey, A M Capponi, R Brewster, M F Schulz, E Kawashima, S J Arkinstall

{"title":"Recombinant bovine neurokinin-2 receptor stably expressed in Chinese hamster ovary cells couples to multiple signal transduction pathways.","authors":"H R Eistetter, D J Church, A Mills, P P Godfrey, A M Capponi, R Brewster, M F Schulz, E Kawashima, S J Arkinstall","doi":"10.1091/mbc.2.10.767","DOIUrl":"https://doi.org/10.1091/mbc.2.10.767","url":null,"abstract":"Neurokinins are a family of neuropeptides with widespread distribution mediating a broad spectrum of physiological actions through three distinct receptor subtypes: NK-1, NK-2, and NK-3. We investigated some of the second messenger and cellular processes under control by the recombinant bovine NK-2 receptor stably expressed in Chinese hamster ovary cells. In this system the NK-2 receptor displays its expected pharmacological characteristics, and the physiological agonist neurokinin A stimulates several cellular responses. These include 1) transient inositol 1,4,5-trisphosphate (IP3) formation and Ca2+ mobilization, 2) increased out put of arachidonic acid and prostaglandin E2 (PGE2), 3) enhanced cyclic AMP (cAMP) generation, 4) increased de novo DNA synthesis, and 5) an induction of the \"immediate early\" genes c-fos and c-jun. Although NK-2 receptor-mediated IP3 formation involves activation of a pertussis toxin-insensitive G-protein, increased cAMP production is largely a secondary response and can be at least partially attributed to autocrine stimulation by endogenously generated eicosanoids, particularly PGE2. This is the first demonstration that a single recombinant neurokinin receptor subtype can regulate, either directly or indirectly, multiple signal transduction pathways and suggests several potential important mediators of neurokinin actions under physiological conditions.","PeriodicalId":9671,"journal":{"name":"Cell regulation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1091/mbc.2.10.767","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12831536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

An ATP-binding membrane protein is required for protein translocation across the endoplasmic reticulum membrane. atp结合膜蛋白是蛋白质跨内质网膜转运所必需的。

Cell regulation Pub Date : 1991-10-01 DOI: 10.1091/mbc.2.10.851

D L Zimmerman, P Walter

引用次数: 17

Developmental regulation of calmodulin gene expression in rat brain and skeletal muscle. 大鼠脑和骨骼肌钙调蛋白基因表达的发育调控。

Cell regulation Pub Date : 1991-10-01 DOI: 10.1091/mbc.2.10.819

J Weinman, B Della Gaspera, A Dautigny, D Pham Dinh, J Wang, H Nojima, S Weinman

引用次数: 47

Epidermal growth factor-stimulated calcium ion transients in individual A431 cells: initiation kinetics and ligand concentration dependence. 表皮生长因子刺激钙离子在单个A431细胞中的瞬态:起始动力学和配体浓度依赖性。

Cell regulation Pub Date : 1991-10-01 DOI: 10.1091/mbc.2.10.827

T E Cheyette, D J Gross

{"title":"Epidermal growth factor-stimulated calcium ion transients in individual A431 cells: initiation kinetics and ligand concentration dependence.","authors":"T E Cheyette, D J Gross","doi":"10.1091/mbc.2.10.827","DOIUrl":"https://doi.org/10.1091/mbc.2.10.827","url":null,"abstract":"The A431 epidermoid carcinoma cell line responds to epidermal growth factor (EGF) stimulation with a number of rapid changes, including alterations in free cytosolic calcium ion concentration ([Ca2+]i). At the single cell level, these changes in [Ca2+]i are known to proceed after a clear lag phase subsequent to EGF stimulus (Gonzalez et al., 1988). The present study explores the dependence on EGF concentration of this early [Ca2+]i signal. High levels of EGF (9.0-4.3 nM) produce a [Ca2+]i spike followed by an elevation of [Ca2+]i above basal levels. The time of initiation of the spike varies from 5 to 9 s at the high dose and from 8 to 32 s at the low dose in cells that respond. A lower level of EGF (1.5 nM) produces [Ca2+]i oscillations with no prolonged elevation over basal [Ca2+]i. The initiation of response at this [EGF] ranges from 20 to 410 s. Intermediate stimulus levels generate [Ca2+]i responses that are kinetic admixtures of these limiting responses. A simple model based on the enzymatically amplified signal cascade from ligand binding through Ca2+ release or influx is examined. The model predicts a prolonged lag phase followed by a rapid increase in the [CA2+]i signal that compares favorably with the data reported here.","PeriodicalId":9671,"journal":{"name":"Cell regulation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1091/mbc.2.10.827","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12963654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Basic fibroblast growth factor requires a long-lasting activation of protein kinase C to induce cell proliferation in transformed fetal bovine aortic endothelial cells. 碱性成纤维细胞生长因子需要蛋白激酶C的长期激活来诱导转化胎牛主动脉内皮细胞的细胞增殖。

Cell regulation Pub Date : 1991-09-01 DOI: 10.1091/mbc.2.9.719

M Presta, L Tiberio, M Rusnati, P Dell'Era, G Ragnotti

{"title":"Basic fibroblast growth factor requires a long-lasting activation of protein kinase C to induce cell proliferation in transformed fetal bovine aortic endothelial cells.","authors":"M Presta, L Tiberio, M Rusnati, P Dell'Era, G Ragnotti","doi":"10.1091/mbc.2.9.719","DOIUrl":"https://doi.org/10.1091/mbc.2.9.719","url":null,"abstract":"Basic fibroblast growth factor (bFGF) induces a protein kinase C (PKC)-dependent mitogenic response in transformed fetal bovine aortic endothelial GM 7373 cells. A long-lasting interaction of bFGF with the cell is required to induce cell proliferation. bFGF-treated cells are in fact committed to proliferate only after they have entered the phase S of the cell cycle, 12-14 h after the beginning of bFGF treatment. Before that time, the mitogenic response to bFGF is abolished by 1) removal of extracellular bFGF by suramin, 2) addition of neutralizing anti-bFGF antibodies to the culture medium, 3) inhibition of PKC activity by the protein kinase inhibitor H-7, and 4) down-regulation of PKC by cotreatment with phorbol ester. Thus the requirement for a prolonged interaction of bFGF with the cell reflects the requirement for a prolonged activation of PKC. Similar conclusions can be drawn for the PKC activators 12-O-tetradecanoyl phorbol 13-acetate and 1,2-dioctanoyl-sn-glycerol. The two molecules require 16 and 6 h, respectively, of activation of PKC to induce 50% of maximal cell proliferation. The requirement for a long-lasting activation of PKC appears to be a mechanism for the control of cell proliferation capable of discriminating among transient nonmitogenic stimuli and long-lasting mitogenic stimuli.","PeriodicalId":9671,"journal":{"name":"Cell regulation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1091/mbc.2.9.719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12906296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 66