Cardioscience最新文献_第9页

Cardioscience Pub Date : 1993-06-01

C Guarnieri, C Muscari, C Finelli, C M Caldarera

引用次数: 0

Isolation of human ventricular and atrial cardiomyocytes: technical note. 人心室和心房心肌细胞的分离:技术说明。

Cardioscience Pub Date : 1993-06-01

P P Nánási, A Varró, D A Lathrop

引用次数: 0

Heterogeneity of smooth muscle in bovine coronary arteries. 牛冠状动脉平滑肌的异质性。

Cardioscience Pub Date : 1993-06-01

A Chiavegato, L Giuriato, M Scatena, S Sartore

{"title":"Heterogeneity of smooth muscle in bovine coronary arteries.","authors":"A Chiavegato, L Giuriato, M Scatena, S Sartore","doi":"","DOIUrl":"","url":null,"abstract":"The monoclonal antibodies TT-1 against troponin T and TI-1 against troponin I of cardiac muscle, together with Western blots and immunofluorescence techniques, were used to evaluate the presence of troponin-like antigens in the smooth muscle cells of bovine coronary arteries. The walls of the left coronary artery before the bifurcation, its circumflex and descending branches, the right coronary artery, and the small branches of coronary arteries and the intramural arterial vessels were found to be homogeneously stained by the TT-1 antibody. Conversely, with the TI-1 antibody, differences were observed in the distribution of the TnI-like antigen among the smooth muscle cells of extramural and intramural vessels. Heterogeneity of the smooth muscle cells of the coronary artery was also evident in the medial layer of large vessels near the branching points, where an intimal thickening was consistently observed. Double immunofluorescence assays with anti-troponin antibodies in vivo and in vitro indicated a distinct intracellular localization of troponin T and troponin I immunoreactivities in vascular smooth muscle cells. The data are compatible with the existence of distinct troponin-like antigens in smooth muscle cells of bovine coronary arteries whose distribution is in part related to the extramural or intramural localization of blood vessels.","PeriodicalId":9629,"journal":{"name":"Cardioscience","volume":"4 2","pages":"75-84"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19333971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Mg2+ on the in vitro responsiveness of adenylate cyclase from hearts of aging rats. Mg2+对衰老大鼠心脏腺苷酸环化酶体外反应性的影响。

Cardioscience Pub Date : 1993-06-01

C Pignatti, B Tantini, M L Zanfanti, P Sacchi, C Clo

{"title":"Influence of Mg2+ on the in vitro responsiveness of adenylate cyclase from hearts of aging rats.","authors":"C Pignatti, B Tantini, M L Zanfanti, P Sacchi, C Clo","doi":"","DOIUrl":"","url":null,"abstract":"The influence of [Mg2+] on the basal or stimulated activity of adenylate cyclase from the hearts of young (1 month old) and aged (24 months old) rats has been investigated in vitro. The basal activity of cardiac adenylate cyclase, and its responsiveness to stimulatory or inhibitory effectors, declined with age. This is probably due to alterations at the catalytic moiety of the signal transduction system, such as an impairment in the affinity of the catalytic moiety for ATP and a lower capacity of the catalytic moiety to bind activated stimulatory (Gs) or inhibitory (Gi) guanine nucleotide binding proteins. Compared to the enzyme from the heart of aged rats, unstimulated adenylate cyclase from the heart of young rats was more sensitive to an increase in [Mg2+] in the incubation mixture, as shown by a greater increase in basal activity and in the affinity of the enzyme for ATP. An increase in [Mg2+] counteracted the inhibitory effect of spermine on adenylate cyclase more effectively in young rats than in aged rats. On the other hand, an increase in [Mg2+] facilitated the stimulation of adenylate cyclase by Gpp(NH)p, isoproterenol and forskolin more in aged rats than in young rats. GDP beta S prevented the positive effect of high [Mg2+] on the stimulation of adenylate cyclase by forskolin, suggesting that an increased [Mg2+] favors the activation of Gs or the formation of functional complexes between the catalytic moiety and Gs. We suggest that aging leads to a higher requirement for Mg2+ at the allosteric site on the catalytic moiety whose occupancy is essential for the full expression of stimulated activity.","PeriodicalId":9629,"journal":{"name":"Cardioscience","volume":"4 2","pages":"105-9"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19333966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental changes in the myosin composition of guinea pig ventricular muscle. Relation to thyroid state and mechanical properties. 豚鼠心室肌肌球蛋白组成的发育变化。甲状腺状态与力学性能的关系。

Cardioscience Pub Date : 1993-06-01

G Kessler-Icekson, H Schlesinger, J Leger, J J Leger, B Felzen, S Mager, O Binah

{"title":"Developmental changes in the myosin composition of guinea pig ventricular muscle. Relation to thyroid state and mechanical properties.","authors":"G Kessler-Icekson, H Schlesinger, J Leger, J J Leger, B Felzen, S Mager, O Binah","doi":"","DOIUrl":"","url":null,"abstract":"In a variety of mammalian species, thyroid hormone regulates the contractile properties of the heart as well as the expression of the alpha and beta heavy chains of myosin. We have previously shown that the plasma levels of thyroid hormone reach a peak immediately after birth in guinea pigs and decline with maturation. We therefore studied age-related changes in the expression of the myosin heavy chains in the guinea pig ventricle in relation to the ventricular mechanical properties and the levels of thyroid hormone. The composition of the myosin heavy chains was characterized by gel electrophoresis and immunoblotting. Anti-beta-chain antibody stained equally myosins from newborns (0-5 days) and adults (75-90 days), while anti-alpha-chain positively decorated only the myosins of euthyroid newborns or of hyperthyroid adults, but not myosins of embryos, hypothyroid newborns or hypothyroid adults. Myosin of euthyroid adults was faintly stained by anti-alpha-chain. The alterations in the composition of myosin corresponded with the \"thyroid state\" of these groups. The plasma levels of total T3 were 24.3 +/- 2.7, 9.04 +/- 1.2 and 139.0 +/- 9.3 ng/dl (mean +/- SEM) in the euthyroid, hypothyroid and hyperthyroid adults, respectively. In euthyroid and hypothyroid newborns, the plasma levels of T3 were 56.5 +/- 11.9 and 26.5 +/- 9.8 ng/dl, respectively. Within each age group the thyroid state corresponded with maximal twitch tension (Tmax), rates of development of tension and relaxation, time to peak tension and rate of activation.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":9629,"journal":{"name":"Cardioscience","volume":"4 2","pages":"69-74"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19333970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-dependent changes in myocardial susceptibility to ischemic injury. 心肌对缺血性损伤易感性的年龄依赖性变化。

Cardioscience Pub Date : 1993-06-01

E Riva, D J Hearse

{"title":"Age-dependent changes in myocardial susceptibility to ischemic injury.","authors":"E Riva, D J Hearse","doi":"","DOIUrl":"","url":null,"abstract":"We studied the relation between age and the heart's ability to tolerate ischemia. Groups of 5-7 isolated Langendorff perfused hearts from neonatal (5 days old), immature (11-44 days old), and adult (54 days old) rats, were subjected to 60 minutes of global ischemia and 60 minutes of reperfusion at 37 degrees C. Pre-ischemic left ventricular developed pressure was 54 +/- 1, 56 +/- 2, 59 +/- 4, 80 +/- 3, 91 +/- 9, 91 +/- 8, 94 +/- 3, 101 +/- 10, 107 +/- 8 and 132 +/- 4 mmHg in the 5, 11, 14, 18, 23, 26, 33, 40, 44, and 54 day old groups, respectively. Left ventricular developed pressure recovered to 65 +/- 13, 69 +/- 4, 103 +/- 14, 84 +/- 10, 81 +/- 3, 59 +/- 11, 42 +/- 10, 34 +/- 6, 34 +/- 6 and 25 +/- 7% respectively. Ischemia-induced contracture was greater in adult hearts and the time-to-onset and the time-to-peak contracture decreased with increasing age. Leakage of creatine kinase after ischemia correlated poorly with the recovery of contraction. Left ventricular end-diastolic pressure, at the end of 60 minutes of reperfusion, increased in an age-dependent manner from a pre-ischemic value which was set at 2-8 mmHg to 14 +/- 4, 22 +/- 2, 21 +/- 5, 27 +/- 3, 47 +/- 16, 52 +/- 7, 71 +/- 7, 81 +/- 9, 73 +/- 5, and 82 +/- 8 mmHg in the 5, 11, 14, 18, 23, 26, 33, 40, 44, and 54 day old groups, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":9629,"journal":{"name":"Cardioscience","volume":"4 2","pages":"85-92"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19333972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coronary hemodynamics during isometric handgrip and atrial pacing in patients with angina pectoris compared to healthy men. 心绞痛患者与健康男性在等距握力和心房起搏时的冠状动脉血流动力学

Cardioscience Pub Date : 1993-06-01

L Kaijser, B Berglund

{"title":"Coronary hemodynamics during isometric handgrip and atrial pacing in patients with angina pectoris compared to healthy men.","authors":"L Kaijser, B Berglund","doi":"","DOIUrl":"","url":null,"abstract":"The responses in coronary blood flow and the relative myocardial extraction of oxygen to atrial pacing (causing mainly an increase in heart rate) and isometric handgrip (causing mainly an increase in pressure) were studied in patients with angina and in young and middle-aged healthy men. The myocardial extraction of oxygen and coronary sinus flow were measured by catheterizing the coronary sinus, using a catheter with thermistors for the measurement of flow by thermodilution and electrodes for atrial pacing. In the healthy men the increase in the consumption of oxygen was covered entirely by an increased coronary blood flow during both provocations, with no change in the arteriovenous oxygen difference during handgrip and a decrease during pacing. There was no significant difference in the reaction between younger and older men. In the patients with angina the increased consumption of oxygen during pacing was covered by an increased coronary blood flow with an unaltered arteriovenous oxygen difference, while during handgrip both the coronary blood flow and arteriovenous oxygen difference increased. It is concluded that during handgrip, compared to the artificial increase in heart rate, the myocardium is more dependent upon an increased relative extraction of oxygen to cover an increase in the requirement for oxygen. This may be due to a higher intramyocardial pressure. Patients with coronary heart disease are more dependent than healthy men on an increase in the relative myocardial extraction of oxygen. This may be related to a lower effective perfusion pressure because of the coronary arterial obstructions or to an increased intramyocardial pressure and a relatively shorter duration of diastole because of the impaired cardiac function.","PeriodicalId":9629,"journal":{"name":"Cardioscience","volume":"4 2","pages":"99-104"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19334563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myocardial infarction, cardiac anatomy and ventricular loading. 心肌梗死，心脏解剖和心室负荷。

Cardioscience Pub Date : 1993-06-01

P Anversa, G Olivetti, P Li, M V Herman, J M Capasso

引用次数: 0

Functional and molecular changes in FoF1 ATP-synthase of cardiac muscle during aging. 衰老过程中心肌FoF1 atp合酶的功能及分子变化。

Cardioscience Pub Date : 1993-06-01

F Guerrieri, G Capozza, A Fratello, F Zanotti, S Papa

引用次数: 0

Prodynorphin mRNA is synthesized in adult cultured rat ventricular cardiomyocytes. 前啡肽mRNA在培养的成年大鼠心室心肌细胞中合成。

Cardioscience Pub Date : 1993-03-01

C Ventura, M Canossa, I Vaona, L Carboni, C M Caldarera, S Spampinato, C Guarnieri

引用次数: 0