Carlsberg Research Communications最新文献_第3页

Amino acid sequence of the 9-kDa iron-sulfur protein of photosystem I in barley. 大麦光系统I 9-kDa铁硫蛋白氨基酸序列。

Carlsberg Research Communications Pub Date : 1989-01-01 DOI: 10.1007/BF02910468

H V Scheller, I Svendsen, B L Møller

引用次数: 16

Substrate specificity of proteinase yscA from saccharomyces cerevisiae. 酿酒酵母蛋白酶yscA的底物特异性。

Carlsberg Research Communications Pub Date : 1989-01-01 DOI: 10.1007/BF02908301

T Dreyer

引用次数: 23

Characterization and in vitro expression of the cytochrome b-559 genes of barley. II. In vitro transcription and translation. 大麦细胞色素b-559基因的鉴定及体外表达。2体外转录和翻译。

Carlsberg Research Communications Pub Date : 1988-01-01 DOI: 10.1007/BF02907180

K Krupinska

{"title":"Characterization and in vitro expression of the cytochrome b-559 genes of barley. II. In vitro transcription and translation.","authors":"K Krupinska","doi":"10.1007/BF02907180","DOIUrl":"https://doi.org/10.1007/BF02907180","url":null,"abstract":"The two cytochrome b-559 apoproteins of 9.4 kD and 4.5 kD molecular weight have been expressed in vitro using DNA templates containing either the two genes psbE and psbF in tandem or the individual genes. Transcription with E. coli RNA-polymerase or SP6 RNA-polymerase has been followed by translation in E. coli derived lysates. Simultaneous as well as independent synthesis of the apoproteins is possible. A 9.4 kD in vitro translation product has been identified as apoprotein I by immunoprecipitation with a monoclonal antibody specific for the C-terminal part of the 9.4 kD apoprotein of cytochrome b-559. The isolated psbF gene directs the synthesis of a translation product with a molecular weight of 4.5 kD corresponding to apoprotein II. Expression of the psbE gene requires the presence of endogenous regulatory sequences 5' upstream of psbE, while this is not the case for psbF. Additional in vitro translation products of 5.7 and 2.4 kD molecular weights are synthesized and probably translated from two reading frames starting with two different out-of-phase ATG codons in the nucleotide sequence of the psbE gene.","PeriodicalId":9616,"journal":{"name":"Carlsberg Research Communications","volume":"53 4","pages":"233-46"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02907180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14382580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Barley powdery mildew “Invertase” is an alpha-glucosidase 大麦白粉病“转化酶”是一种α -葡萄糖苷酶

Carlsberg Research Communications Pub Date : 1988-01-01 DOI: 10.1007/BF02904469

I. Donaldson, J. Jørgensen

引用次数: 6

Chromosome pairing and synaptonemal complex formation in hexaploid wheat, nullisomic for chromosome 5B 小麦六倍体染色体配对和突触复合体的形成，5B染色体为零体

Carlsberg Research Communications Pub Date : 1988-01-01 DOI: 10.1007/BF02907175

P. Holm

引用次数: 33

beta-Ketoacyl-ACP synthase I of Escherichia coli: nucleotide sequence of the fabB gene and identification of the cerulenin binding residue. 大肠杆菌β -酮酰基- acp合成酶ⅰ:fabB基因的核苷酸序列及蓝绿蛋白结合残基的鉴定。

Carlsberg Research Communications Pub Date : 1988-01-01 DOI: 10.1007/BF02983311

S Kauppinen, M Siggaard-Andersen, P von Wettstein-Knowles

引用次数: 132

DNA sequence polymorphisms in the genus Saccharomyces. V. Cloning and characterization of a LEU2 gene from S. carlsbergensis. 酵母菌属的DNA序列多态性。5、carlsbergens菌株LEU2基因的克隆与鉴定。

Carlsberg Research Communications Pub Date : 1988-01-01 DOI: 10.1007/BF02904408

G P Casey, M B Pedersen

{"title":"DNA sequence polymorphisms in the genus Saccharomyces. V. Cloning and characterization of a LEU2 gene from S. carlsbergensis.","authors":"G P Casey, M B Pedersen","doi":"10.1007/BF02904408","DOIUrl":"https://doi.org/10.1007/BF02904408","url":null,"abstract":"Saccharomyces carlsbergensis strains used in the production of lager beer are structurally heterozygous in most genetic loci studied to date. Previous studies have shown that the genotype of lager yeast contains two types of genomes, one of which is derived from S. cerevisiae and the other reveals similarities to the genomes of S. bayanus and S. monacensis. Genes of homeologous chromosomes can be distinguished by characteristic restriction fragment patterns. This is true also for the LEU2 genes which encode the beta-isopropylmalate dehydrogenase and are located on chromosomes III. In the present work a LEU2 gene from S. carlsbergensis has been cloned and characterized. The cloned 2.6 kb LEU2 region complements the S. cerevisiae leu2-3 leu2-112 double mutation. The restriction endonuclease site map of the isolated S. carlsbergensis LEU2 gene is different from that of the S. cerevisiae LEU2 gene. Electrophoretic chromosome separation, as well as karl mediated transfer of single chromosomes into S. cerevisiae strains, has shown that the S. carlsbergensis specific LEU2 gene is located on a chromosome III which carries the carlsbergensis specific HIS4 gene. The cloned LEU2 gene shows preferential molecular hybridization to one of the two LEU2 structural alleles present in lager strains, an allele which is also present in type strains of S. bayanus, S. carlsbergensis, S. monacensis and S. uvarum.","PeriodicalId":9616,"journal":{"name":"Carlsberg Research Communications","volume":"53 3","pages":"209-19"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02904408","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14385765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Quantification of high molecular weight (1→3)(1→4)-β-d-glucan using Calcofluor complex formation and flow injection analysis. II. determination of total β-glucan content of barley and malt 高分子量(1→3)(1→4)-β-d-葡聚糖的定量分析。2大麦、麦芽总β-葡聚糖含量的测定

Carlsberg Research Communications Pub Date : 1988-01-01 DOI: 10.1007/BF02904434

K. G. Jørgensen, S. Aastrup

引用次数: 50

Primary structure of barley genes encoding quinone and chlorophyll a binding proteins of photosystem II. 大麦光系统II中醌和叶绿素a结合蛋白编码基因的一级结构。

Carlsberg Research Communications Pub Date : 1988-01-01 DOI: 10.1007/BF02907182

E M Neumann

{"title":"Primary structure of barley genes encoding quinone and chlorophyll a binding proteins of photosystem II.","authors":"E M Neumann","doi":"10.1007/BF02907182","DOIUrl":"https://doi.org/10.1007/BF02907182","url":null,"abstract":"The psbA, psbD and psbC genes encoding the quinone binding D-1 and D-2 apoproteins and the 44 kD chlorophyll a-apoprotein 3 have been located in the chloroplast genome of barley. They are found on a 23 kbp SalI restriction endonuclease fragment in the large single copy region of the chloroplast DNA adjacent to the inverted repeat. As in other species the psbD and psbC genes have reading frames which overlap by 53 bp. They are transcribed in the same direction but translated with a frameshift of one nucleotide. Ten amino acid substitutions are found among the 18 N-terminal residues of the D-2 polypeptide if barley, spinach, tobacco, pea and the liverwort Marchantia are compared. Only 8 substitutions are present among the 335 other residues of the D-2 polypeptide. The amino acid residues located in the putative binding site for the special pair reaction center chlorophyll and the residues probably serving as ligands to non-heme iron in the D-1 and D-2 proteins of barley are strictly conserved when compared to those of purple bacteria and of other higher plants. Identity is also observed for the residues of importance in the binding of quinones.","PeriodicalId":9616,"journal":{"name":"Carlsberg Research Communications","volume":"53 4","pages":"259-75"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02907182","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14208228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The effect of chromosome 5B on synapsis and chiasma formation in wheat, triticum aestivum cv. Chinese Spring 5B染色体对小麦突触和交叉形成的影响。中国的春天

Carlsberg Research Communications Pub Date : 1988-01-01 DOI: 10.1007/BF02907179

P. Holm, Xingzhi Wang

引用次数: 93