Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies最新文献

{"title":"LC-QTOF-ESI(+)/MS metabolomic profile analysis applied to identify blood biomarkers of benign hyperplasia and prostate cancer.","authors":"Ramona Maria Maxim, Florina Romanciuc, N. Crisan, I. Andras, C. Socaciu","doi":"10.15835/BUASVMCN-ASB:11652","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11652","url":null,"abstract":"Prostate cancer (PCa) is the second leading cause of mortality in men, the present diagnosis method being based on serum prostate-specific antigen (PSA) screening, with low specificity and overestimated values. A combined untargeted and targeted metabolomic study of metabolites from blood serum samples collected from healthy (n=11), hyperplasia (n=39) and prostate cancer (n=83) patients is presented, using the HPLC-(ESI+) QTOF-MS analysis. The profile of blood serum samples provided complementary information obtained from Base Peak Chromatograms and Dissect chromatograms. Based on dissect chromatograms, two different methods of statistical analysis were used, either based on the instrument software with automated alignments with/without normalization (Profile Analysis), or based on manual alignment followed by statistical analysis (Unscrambler10.X software). Both methods used the unsupervised Principal Component Analysis which discriminated between normal, hyperplasia and cancer patients. The second method allowed a better discrimination between groups, by qualitative and quantitative parameters (m/z values versus peak areas) and better possibilities to identify the molecules responsible for such discriminations.Considering the retention time interval (6-17 min), four molecules to be considered as putative biomarkers for hyperplasia or prostate cancer were identified: Prostaglandins E2/G2, Pregnenolone/ethyltestosterone, Lysophosphatidylcholine18:2/0:0, Galactosylceramide (18:1/24:1).By using larger patient cohorts and optimizing the data processing and chemometric analysis, more reliable biomarkers for prostate hyperplasia and cancer can be discovered and quantified. This preliminary study has had promising findings for the implementation and validation of metabolomic targeted analysis in clinical laboratories.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"173-181"},"PeriodicalIF":0.0,"publicationDate":"2015-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15835/BUASVMCN-ASB:11652","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67358725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies regarding the effect of organic fertilizers on a permanent grassland in Petrova, Maramureș.","authors":"A. Botis, G. Mihai, N. Sima, Doru Criste, I. Mihalca, Iulia Medrea, Bogdan Fagadar","doi":"10.15835/BUASVMCN-ASB:11420","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11420","url":null,"abstract":"Grasslands have a great importance for humans around the world being the beginning of many of our food products. Since there is a direct connection between soil, plant and climate, scientist became concerned of this interaction and studies were conducted to describe this connection and to improve output in grasslands with best measures possible. The experiment established in Petrova, Maramureș in the autumn of 2012 is used to obtain information on how different types of manure influence the vegetation and the output on permanent grasslands. Since there are other studies in this field, in different areas of Romania and in other countries, results can be compared so that a greater picture can be made on how organic fertilizers influence the quality and quantity of forage.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"240-244"},"PeriodicalIF":0.0,"publicationDate":"2015-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67356796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics tools for metabolomic data processing and analysis using untargeted liquid chromatography coupled with mass spectrometry.","authors":"A. Lazar, Florina Romanciuc, M. Socaciu, C. Socaciu","doi":"10.15835/BUASVMCN-ASB:11536","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11536","url":null,"abstract":"  Metabolomics is an important “omics” technology, complementary to genomics and proteomics, as parts of systems biology, giving information (qualitative fingerprints and quantitative profiling) as a mirror of cell and extracellular metabolic activity. A cohort of small metabolites are involved in the control and regulation of cellular functions, as intermediates or final products, their presence or levels being useful for the early diagnosis of different pathologies. Bioinformatics tools are mandatory for a future “computational” metabolomics, needed to manage large number of experimentally acquired data obtained from biological samples (plants, animal or human tissues). This review presents updated information about different high-throughput analytical techniques and data acquisition  software (1-2), the pre-processing of data, converted to specific matrices, further processed by specific normalization and alignment  procedures (3), then analysed by statistical univariate and multivariate chemometric and /or statistical techniques (4), identifying biomarkers by comparison with databases (5), and finally elucidating the networks and pathways (6). New software is available for data conversion, pre-processing, alignment algorithms, bucketing, normalization, underlying the challenges and comparisons with international data bases. Finally, the accurate identification of individual molecules as biomarkers, either evaluated by untargeted metabolomics techniques (Principal Component Analysis - PCA), Cluster Analysis - CA) or supervised ones (Partial Least Square Discriminant Analysis (PLS-DA) is presented. The accurate identification of metabolites and their involvement in metabolic networks and pathways became possible by well-established databases (HMDB, LIPID MAPS, KEGG, etc.), to validate all experimental data. Bioinformatics is a sine-qua-non tool, to be used and valorised by untargeted or targeted metabolomics, as an integrated technology in systems biology.     Â","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"103-115"},"PeriodicalIF":0.0,"publicationDate":"2015-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15835/BUASVMCN-ASB:11536","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67358076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive Indices of Rainbow Trout (Oncorhynchus mykiss) Females from a Trout Farm","authors":"Andrada Ihuț, V. Mireșan, D. Cocan, C. Răducu, C. Lațiu, D. Pop","doi":"10.15835/BUASVMCN-ASB:11537","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11537","url":null,"abstract":"Rainbow Trout (Oncorhynchus mykiss) breeding females have been studied from the standpoint of phenotypic characteristics and breeding indices for establishing future protocols of selection and improvement. The relative fecundation (RF) has been determined to a medium value of 335.88 ± 17.723 g and a variability coefficient of 52.77%. The high value of the variability coefficient is due to the fact that this is the first breeding generation of trout nursery and has not been submitted to any selection and improvement activities up to the present. From the breeding performance standpoint, the medium value of the number of eggs (TNE) obtained has been of 8127.44 ± 478.532, the medium egg weight (EW) has been of 0.06 ± 0.001g, the medium diameter of one egg (ED) = 4.16 ± 0.029 mm and the medium volume of one egg (EV) has been of 65.63 ± 1.223 mm3. The following breeding indices have also been calculated: gonadosomatic report (GR), Behning fertilizing coefficient (BFC) and the Williams absolute fecundity indices (WAFI). The outcome results have been the following: GR = 14.82 ± 0.587; BFC = 16.82 ± 0.563; WAFI = 5.77 ± 0.333. The values of the correlations point out the necessity of breeding selection work in order to improve the breeding performances.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"157-161"},"PeriodicalIF":0.0,"publicationDate":"2015-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67358086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some investigations on incidence and infestation level in Cyprinid postodiplostomosis","authors":"O. Negrea, V. Mireșan, C. Răducu, G. Onaciu, O. Negrea, C. Lațiu, C. Daniel","doi":"10.15835/BUASVMCN-ASB:11457","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11457","url":null,"abstract":"In our study, we sampled 82 fish specimens as follows: 62 rudd ( Scardinius erythrophthalmus ), 8 roach (Rutilus rutilus) and 12 silver bream (Blicca bjoerkna). The sampling was done in A¢aga Mare Lake, Cluj County. The investigations took into account the incidence and infestation levels of fish with Posthodiplostomum cuticola metacercaria. The study results show an incidence of parasitosis of 80.60% for rudd, 75.00% for roach and 25.05% for silver bream. Regarding the intensity of infestation (low, medium and high), low levels of infestation are predominant (rudd - 72%; roach 83.30%; silver bream - 66.60%). The preferred body area for metacercaria (in descending order) are as follows: for rudd - 90.00% on the body, 86.00 on the fins and caudal peduncle, and 28.80% on the head; for roach - 50.00% on the body, 66.00% on the fins and caudal peduncle, and 33.00% on the head; for silver bream - 100.00% on the body, 33.00% on the fins and caudal peduncle, and 33.00% on the head.Histopathological sections were taken from musculocutaneous tissue extracted from the dorsal area of fish which was parasitized with cystic metacercariae. The central points of metacercaria is surrounded by a connective structure in the form of a capsule, in which melanocyte-like cells are predominant. Intracytoplasmically, they show a brown-blackish granular pigment which masks the cell nucleus. Perifocally, the inflammatory cell infiltrate is discreet with few mononuclear heterophile cells. Â","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"203-206"},"PeriodicalIF":0.0,"publicationDate":"2015-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67357425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological Tests of Tumolit and Artemisinin Products on Laboratory Mice and Rats","authors":"C. Oroian, A. Stuparu, V. Gavrila, T. Oroian","doi":"10.15835/BUASVMCN-ASB:11427","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11427","url":null,"abstract":"This paper presents the experimental results establishing the lethal dose (LD) of the two products considered to have antitumor effect:Tumolit and Artemisinin. For testing, we used an experimental design including two species of gnawers, Swiss mice and Wistar rats. Two experimental groups were used, consisting of 20 Swiss male mice each, as well as two groups of 10 Wistar rats/lot. In interpreting the results of the research and in the development of the experimental protocol, we took into account the toxicity scale after Hodge and Sterner. Initial doses were usedof both products at 200mg / kg / body for each mouse and rat, respectively, administered orallyin single-doses. Between each oral dose, there was one day of rest in order to evaluate possible effects. As mentioned in theProtocol, the dose was increased to500mg dose / kg / body,then to 1000 mg / kg / body, 2000mg / kg / body and then to 5000 mg / kg / body. At this last dose, administration was disrupted in mice. For rats, doses were further increased in three innings to 5000 mg / kg / body, 15,000 mg / kg / body concerned 20,000 mg / kg / body in single oral dose.  The administration was done by gavage in a single dose. There were no toxicity effects reported at any dose level applied, which is why we conclude that the two products are practically non-toxic. Â","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"207-210"},"PeriodicalIF":0.0,"publicationDate":"2015-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67357642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Fodder Additives in Feeds on the Production Performance and Egg Quality of Laying Quails","authors":"T. Măldărășanu, A. Șara, M. Bențea","doi":"10.15835/BUASVMCN-ASB:11460","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11460","url":null,"abstract":"The research has been carried out on 99 laying quails, randomly distributed in 3 groups, each group consisting of 33 birds. Group 1 received the base diet supplemented with organic Selenium (Sel-Plex) 0.04% + BioMos 0.12%; group 2 received the base diet supplemented with organic Selenium (Sel-Plex) 0.04% + Actigen 0.08, while the control group received the base diet, with no fodder additives. The experimental period was 18 weeks (from 5 weeks to 23 weeks of age). Individual weighing of quails was carried out at the beginning, in the middle and the end of the experimental period. During the experiment, a number of indicators were recorded: body mass evolution, mortality rate, laying intensity, mean egg weight, feed consumption/egg, the weight of the main structural components of eggs and their proportion, egg defects, egg chemical composition and the main physical and morphological indicators of egg quality. The combined use of feed additives (Sel-Plex + Bio-Mos and Sel-Plex + Actigen) led to positive results that confirm the positive influence of these additives, when used in the nutrition of laying quails.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"162-168"},"PeriodicalIF":0.0,"publicationDate":"2015-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67358252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different Fertilization Systems in the Petrova Ecosystem, Maramureș County. Note 1: The Evolution of Forage Production","authors":"Doru Criste, G. Mihai, N. Sima, A. Botis, Iulia Medrea, Bogdan Fagadar","doi":"10.15835/BUASVMCN-ASB:11485","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11485","url":null,"abstract":"Set on a Festuca rubra - Agrostis capillaris (Agrostis tenuis) grassland, at a height of 628 m in the MaramureE™ Depression, the experiment was carried out by means of the randomized blocks method. We performed a bifactorial experiment with factor A as the amendment, and factor B the level of fertilization.As a result of fertilization throughout the study period using different doses in accordance with the experiment protocol, improvements were observed from one year to the next. The yield grew significantly on the fertilized variants compared to the witness lots, as seen in the dry matter content. The best results were obtained in the organic-mineral fertilized variants.Keeping in mind resource preservation, environment protection and biodiversity conservation, a good strategy regarding these ecological and economic concerns is the use of organic fertilizers on permanent grasslands. Concerning yield growth, the most effective method is the use of mineral and organic-mineral fertilization.The paper presents the evolution in time of the annual forage production, while data about the floristic composition will be presented in a future paper.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"148-152"},"PeriodicalIF":0.0,"publicationDate":"2015-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67357907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of traditionally obtained buffalo cheese.","authors":"A. Coroian, C. Coroian, V. Mireșan, C. Răducu, Luisa Andronie, D. Cocan, Z. Marchiș, O. Negrea","doi":"10.15835/BUASVMCN-ASB:11553","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11553","url":null,"abstract":"In the past decades buffalo milk has attracted growing interest in most countries, especially due to its nutrition characteristics. In Romania, buffalo milk and milk products are mainly considered local and traditional resources, and play an important role in enhancing livestock biodiversity. Currently, the overall availability of milk from all dairy animal species, as well as the possibility of obtaining new products must be analysed. Buffalo milk composition is influenced by season. Milk fat level in autumn is 7.95%, and in the summer it is 7.29%. The highest mean values for the cheese were recorded in the spring season, with a fat and protein content of 41.02% and 21.34%, respectively. In autumn, the fat content is at 36.92%, and protein is at 19.81% . Â","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"255-256"},"PeriodicalIF":0.0,"publicationDate":"2015-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67357935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insects as an alternative protein source for animal feeding: a short review about chemical composition.","authors":"V. Vrabec, M. Kulma, D. Cocan","doi":"10.15835/BUASVMCN-ASB:11656","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11656","url":null,"abstract":"Currently, insects are considered as a potential substitute for fishmeal and Currently, insects are considered as a potential substitute for fishmeal and soybean meal in feeding mixtures for farm animals. However, detailed information regarding insects’ nutritional values is available only for some species. We suggested criteria for insect suitability to mass production and found 15 species from 5 orders which meet our requirements: Blattaria: Blaptica dubia, Blatta lateralis, Eublaberus distanti, Coleoptera: Alphitobius diaperinus, Tenebrio molitor, Zophobas morio, Diptera: Hermetia illucens, Musca domestica, Lepidoptera: Antheraea assamensis, Bombyx mori, Galleria mellonela, Samia riciini, Orthoptera: Acheta domestica, Locusta migratoria, Zonocerus variegates. We have collected available information about their nutritional composition and compared it to soybean meal and fishmeal. Protein content was found to be similar to (or slightly higher than in) soybean and fish meals. In terms of protein quality, it was found that insect protein composition is more similar to soybean protein or fishmeal with low protein concentration, than to that of high concentration fishmeal or casein. Due to highest lysine and metionin contents, we recommend Musca domestica and Samia riciini as most suitable protein sources for poultry and pigs feeding","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":"72 1","pages":"116-126"},"PeriodicalIF":0.0,"publicationDate":"2015-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15835/BUASVMCN-ASB:11656","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67358365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}