Canadian Journal of Plant Pathology最新文献_第10页

Detection and quantification of Sclerotinia sclerotiorum infestation of canola petals using a clearing and staining technique 用清除和染色技术检测和定量油菜花瓣中的核盘菌感染

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-08-31 DOI: 10.1080/07060661.2022.2119603

K. Jayasena, A. van Burgel, J. Galloway, G. Thomas

引用次数: 0

Gene and lncRNA expression patterns associated with Qfhs.ndsu-3AS Fusarium head blight resistance QTL in durum wheat 硬粒小麦抗枯萎病QTL相关基因和lncRNA表达模式

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-08-25 DOI: 10.1080/07060661.2022.2091663

D. Soresi, Marina L Díaz, P. Bagnaresi, C. Gallo, A. Carrera

{"title":"Gene and lncRNA expression patterns associated with Qfhs.ndsu-3AS Fusarium head blight resistance QTL in durum wheat","authors":"D. Soresi, Marina L Díaz, P. Bagnaresi, C. Gallo, A. Carrera","doi":"10.1080/07060661.2022.2091663","DOIUrl":"https://doi.org/10.1080/07060661.2022.2091663","url":null,"abstract":"Abstract To identify the transcriptional mechanisms underpinning Fusarium head blight (FHB) resistance in durum wheat, an RNA-seq comparative transcriptome analysis was conducted on spikes 72 h after inoculation of a resistant line Langdon(Dic-3A)10 and a susceptible cultivar ‘Langdon’. Previously, 14 differentially expressed genes (DEGs) were mapped in the region of the quantitative trait locus Qfhs.ndsu-3AS, associated with type II resistance to FHB. Here, we focused on the global response of durum wheat to Fusarium graminearum (Fg) infection, identifying the molecular pathways in which DEGs are involved and lncRNAs with roles in transcriptional and post-transcriptional regulation of gene expression. A set of 921 DEGs was identified using the reference genome of the cultivar ‘Svevo’. Relative to the susceptible cultivar, the resistant line Langdon(Dic-3A)10 showed 452 up-regulated genes and 469 down-regulated genes. Upregulated genes in the resistant line included fungal cell-wall degrading enzymes, transcription factors, and genes involved in kinase signalling, phytohormone pathways, calcium signalling, secondary metabolite biosynthesis and toxin sensitivity. Differentially expressed lncRNAs showed different genome locations: 12 cis intergenic (6 up-regulated in Langdon(Dic-3A)10), 4 trans intergenic (one up-regulated in Langdon(Dic-3A)10), 4 intronic (two up-regulated in Langdon(Dic-3A)10) and 2 putative miRNAs precursors. Possible target genes were predicted for 14 lncRNAs, indicating potential regulative effects on wax and lignin synthesis and ankyrines in the resistant line. In a context where resistance sources against Fg are scarce in durum germplasm, the transcriptomic characterization of a valuable genotype contributes to the understanding of the genetic basis and regulatory network involved in the resistance conferred by Qfhs.ndsu-3AS.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"45 1","pages":"41 - 56"},"PeriodicalIF":2.0,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42816780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and pathogenicity of Fusarium spp. isolates causing root and collar rot on carrot 胡萝卜根茎腐病镰刀菌的鉴定及致病性

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-07-19 DOI: 10.1080/07060661.2022.2103737

C. Pascouau, Corentin Chateau, F. Bastide, T. Le Moullec-Rieu, T. Guillemette, B. Hamon, Sophie Aligon, Agathe Cailleau, Daniel Sochard, J. Gombert, E. Morel, E. Laurent, I. Sérandat, R. Berruyer, P. Poupard

{"title":"Characterization and pathogenicity of Fusarium spp. isolates causing root and collar rot on carrot","authors":"C. Pascouau, Corentin Chateau, F. Bastide, T. Le Moullec-Rieu, T. Guillemette, B. Hamon, Sophie Aligon, Agathe Cailleau, Daniel Sochard, J. Gombert, E. Morel, E. Laurent, I. Sérandat, R. Berruyer, P. Poupard","doi":"10.1080/07060661.2022.2103737","DOIUrl":"https://doi.org/10.1080/07060661.2022.2103737","url":null,"abstract":"Abstract Since 2015, necrosis caused by Fusarium spp. has been regularly observed in carrot seed crops in France. A collection of 53 Fusarium isolates was gathered from lesions on carrot between 2016 and 2019. Morphological characterization at both the macroscopic and microscopic levels and molecular typing based on the ACL1, RPB2 and EF1-α partial gene sequences resulted in the identification of two main groups, corresponding to 22 F. avenaceum and 22 F. tricinctum isolates. In addition, three isolates of F. graminearum, two each of F. oxysporum and F. acuminatum, and one isolate of F. solani were also identified. Dual cultures of Trichoderma spp. (T. atroviride and T. harzianum) and Fusarium spp. (F. tricinctum isolate FT001 and F. avenaceum isolate FA002) did not indicate any antibiosis capabilities of Trichoderma. The in vitro sensitivity of isolates FT001 and FA002 to two fungicides and one natural product was similar, with an efficacy depending on the active ingredient. Prothioconazole + tebuconazole was more effective (mycelial growth reduced by 97% for FT001 and 99.8% for FA002) than fluopyram + trifloxystrobin (mycelial growth reduced by 58% and 56%, respectively). Allium extracts seemed to be a promising alternative to fungicides, since they were almost as efficient as prothioconazole + tebuconazole. Artificial inoculation of durum wheat using three isolates from carrot lesions (FT001, FA002 and F. graminearum FG001) induced plant necrosis. This result suggests that Fusarium pathogens of carrot seed crops may be transmitted to wheat, which is commonly used in rotation with carrot.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"45 1","pages":"76 - 91"},"PeriodicalIF":2.0,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47092872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial communities of sweet cherry (Prunus avium L.) and assessment of their association with cherry slip-skin-maceration disorder 甜樱桃（Prunus avium L.）的微生物群落及其与樱桃滑皮浸渍障碍关系的评价

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-07-14 DOI: 10.1080/07060661.2022.2097315

D. O'gorman, P. Haag, J. Boulé, Glen Healy, Jordan Fraser, M. Walker, J. R. ÚRBEZ-TORRES

{"title":"Microbial communities of sweet cherry (Prunus avium L.) and assessment of their association with cherry slip-skin-maceration disorder","authors":"D. O'gorman, P. Haag, J. Boulé, Glen Healy, Jordan Fraser, M. Walker, J. R. ÚRBEZ-TORRES","doi":"10.1080/07060661.2022.2097315","DOIUrl":"https://doi.org/10.1080/07060661.2022.2097315","url":null,"abstract":"Abstract A relatively new condition of sweet cherry (Prunus avium L.), cherry slip-skin-maceration disorder (cherry-SSMD), presumably associated with yeast species, required a detailed study looking at the microbial communities inhabiting the fruit and blossoms of commercial cherry trees. Cherry-SSMD affects the fruit quality of mainly late harvest sweet cherries and may lead to symptom development that is associated with one or more different, but typically non-pathogenic yeast species. These symptoms may be the result of significant shifts within the fruit’s normal microbiota, and may in turn be influenced by tree fruit phenology and/or environmental factors relating to variable harvest dates. Therefore, the main objective of this study was to conduct field surveys to investigate the microbial species composition on cherry blossoms and fruit. Results obtained from surveys conducted during the 2013 and 2014 growing seasons indicated that while fungal populations remained low, numbers of yeasts and bacteria continued to increase on the surface of developing fruit throughout the growing season. Species composition was also observed to be variable between years and throughout the growing seasons. However, despite general increases in microbial populations of bacteria and yeasts leading up to harvest, no specific pattern was observed to suggest a pathogenic profile that would link either microbial communities, or individual species to cherry-SSMD.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"45 1","pages":"57 - 69"},"PeriodicalIF":2.0,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45728261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First report of rice grain discolouration and leaf blight caused by Pantoea ananatis in the Kuttanad agro-ecosystem, Kerala, India 印度喀拉拉邦Kuttanad农业生态系统中由Pantea ananatis引起的稻米变色和叶枯病的首次报告

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-07-01 DOI: 10.1080/07060661.2022.2096697

T. Reshma, Smitha Balan, C. Dileep

{"title":"First report of rice grain discolouration and leaf blight caused by Pantoea ananatis in the Kuttanad agro-ecosystem, Kerala, India","authors":"T. Reshma, Smitha Balan, C. Dileep","doi":"10.1080/07060661.2022.2096697","DOIUrl":"https://doi.org/10.1080/07060661.2022.2096697","url":null,"abstract":"Abstract Rice is one of the most important staple food crops, and bacterial diseases are among the major limitations to its productivity and yield. Kuttanad is a unique agro-climatic region in Kerala, India, where rice cultivation occurs below sea level. It is recognized as a Globally Important Agricultural Heritage System by the Food and Agriculture Organization of the United Nations. In July 2021, grain discolouration and leaf blight symptoms were noted on rice plants in the field. Leaves developed yellowish to brown lesions, which turned a pale white to straw colour as the disease progressed. Bacteria were isolated from symptomatic leaves and grains. The pathogenicity of a recovered bacterial strain was assessed by artificial inoculation and Koch’s postulates were met. Re-isolation of the pathogenic strain showed high similarity with the originally recovered strain. The 16S rRNA gene was sequenced (accession no. OK398432) and found to have 100% similarity with Pantoea ananatis, an emerging pathogen of rice. To our knowledge, this is the first report of P.ananatis from the Kuttanad region of India.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"45 1","pages":"30 - 34"},"PeriodicalIF":2.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44206231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic structure and genome-wide association study of a genomic panel of two-row, spring barley (Hordeum vulgare L.) with differential reaction to Fusarium head blight (Fusarium graminearum Schwabe) and deoxynivalenol production 二行春大麦(Hordeum vulgare L.)对赤霉病(Fusarium graminearum Schwabe)和脱氧雪腐镰刀菌醇产生差异反应的遗传结构和全基因组关联研究

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-06-29 DOI: 10.1080/07060661.2022.2086925

J. Tucker, A. Brûlé-Babel, C. Hiebert, R. Larios, W. Legge, A. Badea, W. Fernando

{"title":"Genetic structure and genome-wide association study of a genomic panel of two-row, spring barley (Hordeum vulgare L.) with differential reaction to Fusarium head blight (Fusarium graminearum Schwabe) and deoxynivalenol production","authors":"J. Tucker, A. Brûlé-Babel, C. Hiebert, R. Larios, W. Legge, A. Badea, W. Fernando","doi":"10.1080/07060661.2022.2086925","DOIUrl":"https://doi.org/10.1080/07060661.2022.2086925","url":null,"abstract":"Abstract Fusarium head blight (FHB), primarily incited by Fusarium graminearum Schwabe, is the most devastating disease of barley (Hordeum vulgare L.) in Canada. Contaminated grains are unsuitable for use as livestock feed or by the malting industry, primarily due to contamination by mycotoxins such as deoxynivalenol (DON). Reducing DON content has been a long-term goal of barley breeders; however, the complex genetics of resistance and laborious testing required have made it difficult to develop resistant cultivars. An Illumina 50 K SNP beadchip was used to genotype a diverse collection (n = 400) of two-row barley genotypes, selected primarily from North American-breeding programs. This genomic panel was phenotyped in three environments in Manitoba, Canada, over two growing seasons. Genotypes were evaluated for heading date, height, FHB score, and DON content. A genome-wide association study (GWAS) was carried out using a mixed linear model (MLM: Q + K) accounting for population structure (Q) and kinship (K) as covariates. This study characterized the population structure of two-row barley germplasm important to North American-breeding programs. Significant marker-trait associations were identified on all chromosomes for FHB, and on all chromosomesexcept 1 H and 6 H for DON content. Individual marker effects were small, explaining only up to 5% of the phenotypic variation. Genes within genomic regions that were associated with FHB-related traits displayed multiple functions linked with disease resistance. While numerous minor, marker-trait associations were identified, phenotypic evaluations are still the best predictors of these traits, despite their time-consuming nature and dependenceon environmental conditions.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"44 1","pages":"874 - 891"},"PeriodicalIF":2.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46298127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In memoriam / En mémoire: Chuji Hiruki 1931-2021 纪念：Chuji Hiruki 1931-2021

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-06-28 DOI: 10.1080/07060661.2022.2087334

Stephen E. Strelkov

引用次数: 0

Brome grasses represent the primary source of Claviceps purpurea inoculum associated with barley fields in the San Luis Valley of Colorado Brome草是与科罗拉多州圣路易斯山谷的大麦田相关的紫锥菌接种物的主要来源

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-06-16 DOI: 10.1080/07060661.2022.2091041

Stephen A. Wyka, And Kirk Broders

{"title":"Brome grasses represent the primary source of Claviceps purpurea inoculum associated with barley fields in the San Luis Valley of Colorado","authors":"Stephen A. Wyka, And Kirk Broders","doi":"10.1080/07060661.2022.2091041","DOIUrl":"https://doi.org/10.1080/07060661.2022.2091041","url":null,"abstract":"Abstract The fungal pathogen Claviceps purpurea can infect numerous grass species including important crops like barley, rye, and wheat, but the ecology of ergot disease is rarely studied in non-crop grass species. Recent outbreaks of ergot in barley production systems in the western U.S. have implicated nearby grass hosts as reservoirs for inoculum and spread of C. purpurea into the barley crop. We surveyed the prevalence of ergot infections among native, invasive, and weedy grass species near barley fields in the San Luis Valley of Colorado over three years. We found that barley fields with a history of ergot disease were surrounded by a grass community composed largely of smooth brome, that smooth brome was very susceptible to ergot infection and had the highest infection rates among grass species, and that disease severity among the grass community increased during the growing season. Over the study period there was a low incidence of barley infections and this precluded analysing the effects unmanaged grass composition and disease incidence had on within field disease incidence. Our findings indicate that smooth brome represents a significant source of C. purpurea inoculum and inoculum levels increase as the composition of brome grass in the grass community increases. There is sufficient inoculum present in the brome grass population in the San Luis Valley to cause sporadic disease in barley fields when environmental conditions are optimal for infection. Future disease management strategies should take into account the large pool of inoculum in the grass population near barley production fields.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"45 1","pages":"15 - 29"},"PeriodicalIF":2.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49618880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Response of the legume Macrotyloma axillare ‘Java’ to different inoculum levels of Meloidogyne javanica and defence responses 不同接种量的爪哇根结线虫对豆科植物‘爪哇’腋窝巨菌的反应及防御反应

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-06-14 DOI: 10.1080/07060661.2022.2088621

A. Miamoto, A. P. Mattos, A. Calandrelli, L. Rinaldi, T. M. Mioranza, M. T. Silva, L. P. S. Chidichima, C. R. Dias-Arieira

{"title":"Response of the legume Macrotyloma axillare ‘Java’ to different inoculum levels of Meloidogyne javanica and defence responses","authors":"A. Miamoto, A. P. Mattos, A. Calandrelli, L. Rinaldi, T. M. Mioranza, M. T. Silva, L. P. S. Chidichima, C. R. Dias-Arieira","doi":"10.1080/07060661.2022.2088621","DOIUrl":"https://doi.org/10.1080/07060661.2022.2088621","url":null,"abstract":"Abstract This study aimed to examine the relationship between Macrotyloma axillare ‘Java’ and Meloidogyne javanica at different levels of nematode inoculum, and to assess the activity of plant defence enzymes in the presence of nematodes. In Experiment 1, ‘Java’ and soybean (control) were inoculated with 300 to 8000 eggs + second-stage juveniles (J2) of M. javanica in two different periods (Trials 1 and 2). To confirm the results, a third trial was conducted using 1000 to 4000 eggs + J2 per plant. In Experiment 2, β-1,3-glucanase (GLU), phenylalanine ammonia-lyase (PAL), peroxidase (POX), and polyphenol oxidase (PPO) activities were measured in non-inoculated plants and in plants inoculated with 2000 eggs + J2. ‘Java’ presented a reproduction factor (RF) < 1 at all inoculum levels in Trial 1 (RF of 0.17–0.78) and at 2000, 3000, and 6000 eggs + J2 in Trial 2 (RF of 0.47, 0.86, and 0.59, respectively). In Trial 3, ‘Java’ showed a RF < 1 at all inoculum levels except for 3000 eggs + J2 (RF = 1.1). There was no difference in ‘Java’ vegetative variables in relation to inoculum levels in any experiment. In Experiment 2, inoculated plants showed increased GLU, PAL, POX, and PPO activities, when compared with non-inoculated plants. ‘Java’ appears to be moderately resistant to M. javanica, reducing nematode reproduction at different inoculum levels. The plant exhibits increased GLU, PAL, POX, and PPO activities in the presence of M. javanica.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"44 1","pages":"907 - 917"},"PeriodicalIF":2.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44446024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Application of plant growth-promoting rhizobacteria combined with compost as a management strategy against Verticillium dahliae in tomato 植物生长促进根际细菌与堆肥联合防治番茄黄萎病菌的研究

IF 2 4区农林科学

Canadian Journal of Plant Pathology Pub Date : 2022-06-13 DOI: 10.1080/07060661.2022.2089235

Y. Ait Rahou, A. Douira, A. Tahiri, El Modafar Cherkaoui, R. Benkirane, A. Meddich

{"title":"Application of plant growth-promoting rhizobacteria combined with compost as a management strategy against Verticillium dahliae in tomato","authors":"Y. Ait Rahou, A. Douira, A. Tahiri, El Modafar Cherkaoui, R. Benkirane, A. Meddich","doi":"10.1080/07060661.2022.2089235","DOIUrl":"https://doi.org/10.1080/07060661.2022.2089235","url":null,"abstract":"Abstract This study aimed to evaluate the effect of plant growth-promoting rhizobacteria (PGPR) and compost, applied alone or in combination, on tomato resistance to vascular wilt caused by Verticillium dahliae. Improved management of vascular wilt is important for enhanced productivity of greenhouse-grown susceptible tomato plants. The application of compost and PGPR resulted in a reduction in infection parameters compared with untreated plants inoculated with the pathogen, as well as activation of plant defence mechanisms. In the presence of the pathogen, compost application significantly improved the total fresh and dry weights by 97% and 71%, respectively, compared with pathogen-inoculated controls. The combination of compost with PGPR significantly increased chlorophyll fluorescence and stomatal conductance compared with the infected-control. In addition, the combination of compost and PGPR reduced the accumulation of stress markers such as malondialdehyde and hydrogen peroxide by 48% and 77%, respectively, and promoted the activity of antioxidant enzymes. Fruit yield and quality were also significantly improved relative to the control. The results suggest that compost applied alone or in combination with PGPR helps to suppress vascular wilt and to improve the yield and quality of tomatoes.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"44 1","pages":"806 - 827"},"PeriodicalIF":2.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43214775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5