Journal of biomolecular techniques : JBT最新文献

Article Watch: July, 2024. 文章观察2024 年 7 月

Journal of biomolecular techniques : JBT Pub Date : 2024-06-27 eCollection Date: 2024-07-31 DOI: 10.7171/3fc1f5fe.e91d498b

Clive Slaughter

引用次数: 0

Protein Expression Autoinduction in a Cold-Shock Expression System in Escherichia coli. 大肠杆菌冷休克表达系统中的蛋白质表达自诱导。

Journal of biomolecular techniques : JBT Pub Date : 2024-04-29 eCollection Date: 2024-07-31 DOI: 10.7171/3fc1f5fe.76009c9a

Yukino Tsujimoto, Naoto Isono

引用次数: 0

Improved Yield of SPRI Beads-Based Size Selection in the Very High Molecular Weight Range. 提高基于 SPRI 珠的超高分子量范围粒度选择的产量。

Journal of biomolecular techniques : JBT Pub Date : 2024-04-29 eCollection Date: 2024-04-30 DOI: 10.7171/3fc1f5fe.d13e7666

Alexei Stortchevoi, Stuart S Levine

引用次数: 0

Article Watch: April, 2024. 文章观察2024 年 4 月

Journal of biomolecular techniques : JBT Pub Date : 2024-04-25 eCollection Date: 2024-04-30 DOI: 10.7171/3fc1f5fe.56744044

Clive Slaughter

引用次数: 0

Core Exchange: A Professional Development Program for Shared Resource Personnel. 核心交流：共享资源人员专业发展计划。

Journal of biomolecular techniques : JBT Pub Date : 2024-04-25 eCollection Date: 2024-04-30 DOI: 10.7171/3fc1f5fe.1eb341de

Amy F Martinez, Jenny C Schafer, Jennifer Canning, Jayme O'Neal, Kimberly Brown Dahlman

{"title":"Core Exchange: A Professional Development Program for Shared Resource Personnel.","authors":"Amy F Martinez, Jenny C Schafer, Jennifer Canning, Jayme O'Neal, Kimberly Brown Dahlman","doi":"10.7171/3fc1f5fe.1eb341de","DOIUrl":"10.7171/3fc1f5fe.1eb341de","url":null,"abstract":"Introduction/objective: Academic institutions often struggle to meet the unique professional development needs of shared resource personnel, who require business skills, project and people management expertise, and an active, collaborative network of shared resource colleagues.Materials and methods: We launched the Vanderbilt Core Exchange professional development and networking program in 2020. The program was intentionally designed with core personnel input and supports faculty and staff from more than 80 shared resources across Vanderbilt University and Vanderbilt University Medical Center. Resources offered include a quarterly seminar series with both virtual and in-person events, a website for accessing professional development materials and session recordings, and a dedicated online discussion group for core personnel networking.Results: There have been 11 Vanderbilt Core Exchange events to date: 2 in person and 9 virtual. In-person events averaged 35 attendees, and virtual events averaged 45 attendees. Topics included equipment grant writing, marketing, handling difficult conversations, managing different workplace work styles, communication and project management tools, and the importance of self-care. Survey responses collected after each event were highly positive and informed areas of improvement and future event topics.Discussion: This model of local shared resource professional development serves as a template for institutions who desire to create opportunities for collaboration and community building. With a small coordinating committee of dedicated individuals, an institution-wide professional development and networking program can be successfully established even with limited resources.","PeriodicalId":94326,"journal":{"name":"Journal of biomolecular techniques : JBT","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11373896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding a Wastewater-Based Surveillance Methodology for DNA Isolation from a Workflow Optimized for SARS-CoV-2 RNA Quantification. 从优化的 SARS-CoV-2 RNA 定量工作流程中扩展基于废水的 DNA 分离监测方法。

Journal of biomolecular techniques : JBT Pub Date : 2023-12-20 eCollection Date: 2023-12-01 DOI: 10.7171/3fc1f5fe.dfa8d906

Kristina M Babler, Mark E Sharkey, Ayaaz Amirali, Melinda M Boone, Samuel Comerford, Benjamin B Currall, George S Grills, Jennifer Laine, Christopher E Mason, Brian Reding, Stephan Schürer, Mario Stevenson, Dušica Vidović, Sion L Williams, Helena M Solo-Gabriele

{"title":"Expanding a Wastewater-Based Surveillance Methodology for DNA Isolation from a Workflow Optimized for SARS-CoV-2 RNA Quantification.","authors":"Kristina M Babler, Mark E Sharkey, Ayaaz Amirali, Melinda M Boone, Samuel Comerford, Benjamin B Currall, George S Grills, Jennifer Laine, Christopher E Mason, Brian Reding, Stephan Schürer, Mario Stevenson, Dušica Vidović, Sion L Williams, Helena M Solo-Gabriele","doi":"10.7171/3fc1f5fe.dfa8d906","DOIUrl":"10.7171/3fc1f5fe.dfa8d906","url":null,"abstract":"Wastewater-based surveillance (WBS) is a noninvasive, epidemiological strategy for assessing the spread of COVID-19 in communities. This strategy was based upon wastewater RNA measurements of the viral target, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The utility of WBS for assessing the spread of COVID-19 has motivated research to measure targets beyond SARS-CoV-2, including pathogens containing DNA. The objective of this study was to establish the necessary steps for isolating DNA from wastewater by modifying a long-standing RNA-specific extraction workflow optimized for SARS-CoV-2 detection. Modifications were made to the sample concentration process and included an evaluation of bead bashing prior to the extraction of either DNA or RNA. Results showed that bead bashing reduced detection of RNA from wastewater but improved recovery of DNA as assessed by quantitative polymerase chain reaction (qPCR). Bead bashing is therefore not recommended for the quantification of RNA viruses using qPCR. Whereas for Mycobacterium bacterial DNA isolation, bead bashing was necessary for improving qPCR quantification. Overall, we recommend 2 separate workflows, one for RNA viruses that does not include bead bashing and one for other microbes that use bead bashing for DNA isolation. The experimentation done here shows that current-standing WBS program methodologies optimized for SARS-CoV-2 need to be modified and reoptimized to allow for alternative pathogens to be readily detected and monitored, expanding its utility as a tool for public health assessment.","PeriodicalId":94326,"journal":{"name":"Journal of biomolecular techniques : JBT","volume":"34 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10805363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139548063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Article Watch: December, 2023. 文章观察2023 年 12 月

Journal of biomolecular techniques : JBT Pub Date : 2023-12-18 eCollection Date: 2023-12-01 DOI: 10.7171/3fc1f5fe.53f1c3bf

Clive Slaughter

引用次数: 0

Elevating the Educational Mission of "Full-Service" Core Facilities through Formal Biotechnology Workshops. 通过正式生物技术讲习班提升 "全方位服务 "核心设施的教育使命。

Journal of biomolecular techniques : JBT Pub Date : 2023-12-04 eCollection Date: 2023-12-01 DOI: 10.7171/3fc1f5fe.30644720

Emily Ricco, Stacy Nguyen, Victoria Rodriguez, Kieu Pham, Esmanur Tokcan, Sherita Love, Alana Newell, Daniel C Kraushaar

{"title":"Elevating the Educational Mission of \"Full-Service\" Core Facilities through Formal Biotechnology Workshops.","authors":"Emily Ricco, Stacy Nguyen, Victoria Rodriguez, Kieu Pham, Esmanur Tokcan, Sherita Love, Alana Newell, Daniel C Kraushaar","doi":"10.7171/3fc1f5fe.30644720","DOIUrl":"10.7171/3fc1f5fe.30644720","url":null,"abstract":"Core facility laboratories are an essential part of the successful research enterprise of many universities around the world. Core facilities provide state-of-the-art instrumentation and technologies to support research of all faculty, postdocs, and students on a fee-for-service basis. Academic next-generation sequencing cores are typically \"full service\" facilities, and access to and training on their instrumentation is limited to core staff. To address these limitations, we provided graduate students with technical training at our core facility. We developed a 1-week noncredit-bearing workshop and recruited 6 graduate students (N = 6) as part of a pilot program. The program involved online teaching, classroom-based teaching, and hands-on training in next-generation sequencing library preparation and sequencer operation. A post-participation survey revealed highly positive outcomes in terms of skill development and increased awareness of technologies offered by the core facility. A workshop of this scale could be incorporated into the graduate curriculum and extended to core facilities that focus on other technologies. We believe that introducing formal standardized teaching spearheaded by core facilities would improve the graduate student curriculum and hope that this study can provide guidance on curriculum design for similar workshops.","PeriodicalId":94326,"journal":{"name":"Journal of biomolecular techniques : JBT","volume":"34 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10805364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139548059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Survey on Core Flow Cytometry Facilities: Instrument Maintenance, Usage, and Funding. 关于核心流式细胞仪设施的调查：仪器维护、使用和资金。

Journal of biomolecular techniques : JBT Pub Date : 2023-11-30 eCollection Date: 2023-12-01 DOI: 10.7171/3fc1f5fe.557b2117

Sonal Priya, Kathleen Brundage, Ron Orlando

{"title":"A Survey on Core Flow Cytometry Facilities: Instrument Maintenance, Usage, and Funding.","authors":"Sonal Priya, Kathleen Brundage, Ron Orlando","doi":"10.7171/3fc1f5fe.557b2117","DOIUrl":"10.7171/3fc1f5fe.557b2117","url":null,"abstract":"Flow cytometry is a powerful tool that finds applications in various fields such as immunology, molecular biology, cancer biology, virology, and infectious disease monitoring. A significant portion of the research in these disciplines is supported by flow cytometry shared resource laboratories (SRLs). There are several types of flow cytometers available for use in SRLs, including analyzers, sorters, imaging flow cytometers, and mass cytometers. Each type has different challenges when it comes to maintenance and life expectancy. An independent online survey was conducted to better understand instrument maintenance and turnover in flow cytometry SRLs. Questions regarding instrument uptime (availability), its usage, routine maintenance, and cost associated with it were addressed. The respondents also answered questions pertaining to the frequency of deep cleaning of the instrument and quality control. In addition, the survey queried about the source of funding used to purchase the instruments and possible reasons for a replacement. Presented herein are the results compiled from 146 core facilities that provide a look at the operation within a typical SRL, with the responses reflecting researchers' experiences with handling flow cytometers.","PeriodicalId":94326,"journal":{"name":"Journal of biomolecular techniques : JBT","volume":"34 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10805361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139548049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Article Watch: September, 2023. 文章观察:2023年9月。

Journal of biomolecular techniques : JBT Pub Date : 2023-09-25 eCollection Date: 2023-09-30 DOI: 10.7171/3fc1f5fe.bdb707e7

Clive Slaughter

引用次数: 0