Integrative molecular medicine最新文献

{"title":"The value of five - stage combined double - aortic arch incision in prenatal screening of low risk fetus","authors":"Shunan Zhang, Chu Shen, Xin Zhang","doi":"10.15761/imm.1000325","DOIUrl":"https://doi.org/10.15761/imm.1000325","url":null,"abstract":"Objective: To study how to check the difficulty of fetus heart examination in low risk fetus structure screening during the second trimester, and to explore how to use two-dimensional ultrasonography and color Doppler imaging to maximize the detection rate of fetal heart abnormalities The more standardized, effective and rapid inspection methods. Methods: Pregnant women who were screened for fetal structure during 18-24 weeks’ gestation were enrolled in this study. Fetal heart was routinely screened at the third level using fetal two-dimensional section technique and color Doppler imaging. Fetal heart of each case using the “fetal heart ultrasound 5 section basic section combined with double-aortic arch section method” were observed. From the bottom up are: 1, transverse section of the abdomen; 2, dual inflow section; 3, left ventricular outflow tract section; 4, right ventricular outflow tract section; 5, three vascular section; Discovery of Abnormal or Suspicious Anomalies Further append requests for this fetal line of echocardiography are directed to the fourth level of fetal heart screening. And some serious cardiac abnormalities, or combined with multiple organ malformation fetus specimens taken basic anatomical control analysis, and a large number of late follow-up control analysis. Results: This method can effectively improve the detection rate of fetal heart abnormalities. Conclusion: The method is used to instruct the ultrasound doctor to detect the fetal heart abnormality during the screening of the third-level structure of the fetus with low-risk during pregnancy, which can effectively reduce or avoid the missed diagnosis. DOI: 10.29011/2576-9588. 100023","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83378602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absence of bactericidal activity of orally administered wood creosote on human intestinal bacterial flora","authors":"N. Ogata, T. Miura","doi":"10.15761/IMM.1000321","DOIUrl":"https://doi.org/10.15761/IMM.1000321","url":null,"abstract":"[Abstract] Wood creosote is a mixture of simple phenolic compounds that has long been used for over a century as an antidiarrheal medicine. While there are many pharmacological studies that explain its antidiarrheal effects, it has long been a matter of controversy whether the antidiarrheal activity of wood creosote is attributable to its putative bactericidal effect on the human intestinal bacterial flora. The objective of our study was to investigate the putative bactericidal effect of wood creosote in the human intestine when given in an ordinary therapeutic dose. To this end, we used an in vitro test to measure the minimal inhibitory concentration of wood creosote for various bacteria from the human gut. In addition, we also quantified the copy numbers of bacterial 16S ribosomal DNA in the feces of healthy human participants after administration of a therapeutic dose of wood creosote. We determined the minimal inhibitory concentration of wood creosote to be >128 μg/ml, a level far greater than that of commonly used antibacterial agents. Copy numbers of bacterial 16S ribosomal DNA in human feces after the administration of a therapeutic dose of wood creosote did not change significantly ( p > 0.05) from that before dosing. Taken together, we conclude that wood creosote given at an ordinary therapeutic dose has no significant antibacterial effect in the human lower intestine where most of the intestinal bacterial flora resides. Thus, the antidiarrheal properties of wood creosote are not attributable to its effect on the intestinal bacterial flora, but rather to its other effects on the intestine.","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"40 23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88731544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analogous nano compounds of the form M(C8H8)2 exist for M = (Nd, Tb, Pu, Pa, Np, Th, and Yb)-enhanced precatalyst preparation stabilization and initiation (EPPSI) nano molecules","authors":"A. Heidari","doi":"10.15761/imm.1000343","DOIUrl":"https://doi.org/10.15761/imm.1000343","url":null,"abstract":"","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83983712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-301a/b function as oncomiRs in non-small-cell lung cancer","authors":"Takayuki Hirono, Kentaro Jingushi, K. Kitae, T. Nagata, Masami Sato, Kentaro Minami, M. Aoki, A. Takeda, Tadashi Umehara, H. Egawa, Y. Nakatsuji, Yuko Ueda, T. Furukawa, K. Tsujikawa","doi":"10.15761/IMM.1000347","DOIUrl":"https://doi.org/10.15761/IMM.1000347","url":null,"abstract":"Non-small-cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Although recent advances in understanding the pathogenic mechanism of NSCLC has led to the development of targeted treatments, these treatments are only applicable to a limited number of patients. Therefore, the development of a novel therapeutic drug for NSCLC is urgently needed. Here, we focused on miR-301a and miR-301b, belonging to the miR-130 family, because recently, it was reported that miR-130b functions as an oncomiR in NSCLC. The miR-301a and miR-301b were significantly upregulated in NSCLC tissues compared to those in matched-pair adjacent normal lung tissues. Overexpression of miR-301a/b promoted cell proliferation and miR-301b further promoted migration ability in NSCLC cells. Conversely, knockdown of miR-301a and miR-301b significantly suppressed cell proliferation and migration. Moreover, transactivating domain-containing p63 (TAp63), a close relative of the p53 tumor suppressor, was a target gene of both miR-301a and miR-301b in NSCLC cells. These findings showed that miR-301a and miR-301b might function as oncomiRs by targeting TAp63 in NSCLC. Abbreviations: NSCLC: non-small-cell lung cancer; miRNA: microRNA; 3’-UTR: 3’untranslated region; oncomiR: oncogenic miRNA; TAp63: transactivating domain-containing p63.","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79052775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of subcutaneous immunotherapy achieved with a whole-body extract of Pseudomyrmex ant. A case reported in a young woman, in Argentina","authors":"R. Rodriguez, Irañeta Sg, M. Olgiati, L. L. Soprano, K. Mouchián, A. Alonso, V. Duschak","doi":"10.15761/IMM.1000352","DOIUrl":"https://doi.org/10.15761/IMM.1000352","url":null,"abstract":"The aim of this work is to report for the first time a patient case with anaphylaxis by Pseudomyrmex acanthobius /favidulus ant sting, prepare an extract with the wholeant-body, to study their biochemical and immunological properties and to validate the efficacy of the subcutaneous immunotherapy with this non-commercial extract. An argentine woman patient, 19 years old, with background of allergic rhinitis and bronchial asthma in the childhood, suffered repeated episodes of anaphylaxis by non-identified insect’s stings and previous immunotherapies treatment. The entomologic analysis revealed that the aggressor insect was an ant belonging to Pseudomyrmex genera and acanthobius or favidulus species. High serum total levels of IgE (202 UI/ml) were measured by ELISA and a positive 6-mm wheal and flare reaction (extract dilution 1/100000) was revealed by in vivo intradermal test. A sample of the extract proteins fraction was analysed by SDS-PAGE. The silver stained protein bands ranged since 20 to 220 kDa. The patient serum immune-recognized allergenic extract proteins at approximately 160, 90, and a double band at 42/46 kDa prior to desensitization treatment by electro-blotting. Interestingly, post-specific-subcutaneous-immunotherapy the mentioned double band almost disappeared. After a 6-years-treatment, the total IgE serum values strongly decreased and the specific intradermal test was negative up to 1/10 extract dilution. The tolerance to the treatment was good. Altogether, our findings revealed that the immunotherapy performed with the whole-body-Pseudomyrmex ant allergenic extract was highly effective and the IgE specific-double protein allergenic extract bands (42/46 kDa) that disappeared after immunotherapy were responsible for the anaphylactic shock.","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84864418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteoclasts recruitment in internal root resorption associated with external granuloma","authors":"A. Ilea, V. Miclăuș, F. Ruxanda, A. B. Boșca, R. Cȃmpian, N. Petrescu, Anida-Maria Băbțan, A. Mesaroș","doi":"10.15761/IMM.1000341","DOIUrl":"https://doi.org/10.15761/IMM.1000341","url":null,"abstract":"The internal root resorption can be associated with external granuloma. If there is a connection between the two pathological process through a lateral accessory canal is possible that some cells to be recruited from external granuloma. The possible mechanisms involved in osteoclasts recruitment from external granuloma and various dentin resorption patterns in internal root resorption will be discussed based on a case in which the upper lateral incisor with a history of pulp necrosis treated endodontically, developed an internal root resorption in association with external granuloma. Also, a review of literature was performed.","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79016298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The quantum language of the microRNA gene and anti-cancer: with a dynamic computer simulation of human breast cancer drug resistance","authors":"Y. Fujii","doi":"10.15761/IMM.1000346","DOIUrl":"https://doi.org/10.15761/IMM.1000346","url":null,"abstract":"Precision medicine requires for treatments according to the personalized genetic and environmental diversities. In insights of the diversity, individual variation of drug sensitivity would be implied in many factors, one of them would be the microRNA (miRNA) gene. The drug resistance would also be related with alteration of miRNA genotype in the cancer cell via its administrated anticancer drug. Further, natural products as the nutraceuticals could also modulate miRNA expression and promote anticancer effects. In the case of foods, not only foods’ miRNAs but also nutrients and natural products could render changing the phenotype of cells by alteration of miRNA expression. The facts show that environmental quantum energy of therapeutic drugs and foods may have an important role for miRNA gene expression upon the cells to personalize biological reaction under pharmacokinetics, and the new pharmacokinetics may be required through miRNA response. In turn, drugs among individuals treated would be also affected by personal state of quantum energy in the miRNA genes, and the quantum language of miRNA would dynamically adjust re-position of the personalized health. On the contrary, miRNA could control protein expression or epigenetic traits both negatively and positively, or directly and indirectly in the transcription and translation processes. Dysregulation of miRNA induces human cancer. Further, long noncoding RNA (lncRNA) and circular RNA (circRNA) could also act together with miRNAs and would control protein gene expression with miRNA. Participating in cancer development and progression, lncRNAs and circRNAs would directly or indirectly regulate signalling pathways and proliferation processes via miRNA sponging, and function as miRNA reservoir or as binding protein scavengers. The pharmaceutical agents would affect these RNA gene regulators at first in a cell and in vivo. To elucidate new pharmacokinetics of agents, the miRNA-mRNA-lncRNA-circRNA network architecture should be investigated. Thus, we review lncRNA and circRNA functions in cancers. Then, quantum energy relation among miRNA-mRNA-lncRNA-circRNA is discussed. Further, as a sample of drug treatment-related with quantum language of miRNA, drug resistance of human breast cancer was dynamically simulated using the miRNA entangling target sorter (METS). Since we have proved that quantum characters among miRNAs is implicated in oncogenesis, experimental evidences reviewed and dynamic in silico simulation with METS suggested that quantum language of miRNAs may be a common factor through tumorigenesis, anti-cancer and drug resistance.","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88992829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iodine Status in European Women in New Zealand With Moderate Selenium Deficiency","authors":"Ljiljana M Jowitt, Mark Duxbury","doi":"10.15761/IMM.1000302","DOIUrl":"https://doi.org/10.15761/IMM.1000302","url":null,"abstract":"For the optimal function of thyroid gland, adequate intakes of iodine and selenium are required. Since iodine is essential component of the thyroid hormones, its insufficiency leads to inadequate hormone production and further to inadequate tissue response (hypothyroidism), goitre, stillbirth and miscarriages, and growth retardation. According to the World Health Organisation the recommended median urinary iodine concentrations (UIC) are the best indicators of iodine nutrition. The WHO defines iodine sufficiency in an adult population as a median UIC of > 100 μg/L in spot urine samples. Iodine deficiency was and still is a problem in New Zealand. In September 2009 the mandatory fortification of all bread with iodized salt was introduced. Therefore, the primary aim of the study was to determine the levels of iodine in both groups, and the secondary aim of the study was to determine whether there is a relationship between selenium and iodine, and iodine and thyroid hormones in two groups of European women. Urinary iodine concentration was determined in spot samples by the new method called “Fast B”, which is improved Sandell-Kolthoff reaction. The results of the study showed the mean urinary iodine level in the control group and the group of women with the Hashimoto’s thyroiditis was 120.77± 59.35 (median UIC was128.00 μg/L), and 98.64 ± 62.83 (median UIC was 95.00 μg/L), respectively. Estimated daily iodine intake of 150μg/ day was achieved in five participants in the control group, and four participants in the group of women with Hashimoto’s thyroiditis. Estimated median iodine intake in the control group and Hashimoto’s group was 142.22 and 105.55, respectively, indicating mild iodine deficiency. There was no significant relationships found between iodine and selenium, and iodine and thyroid hormones in both groups. The results of the current study are in line with the results from larger studies carried out in New Zealand. Iodine intakes appear to have improved after the mandatory fortification of bread with iodised salt in 2009, although iodine deficiency is still a problem in New Zealand. Using an iodine fortified bread clearly made an impact on the overall iodine intake but not to the expected level. There was no association found between iodine and selenium, and iodine and thyroid hormones. Any possible interaction between selenium and iodine is still unclear.","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"16 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84430775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel Ubc9 -dependent pathway regulates SIRT1- ER-α Axis and BRCA1-associated TNBC lung metastasis.","authors":"Jingyao Xu, Collin Shumate, Yulong Qin, V. Reddy, Yonte Burnam, V. López, J. Okoli, E. P. Reddy, Veena N Rao","doi":"10.15761/IMM.1000298","DOIUrl":"https://doi.org/10.15761/IMM.1000298","url":null,"abstract":"Triple negative breast cancer (TNBC) is a heterogeneous disease and has a higher rate of recurrence and distant metastasis. African-American (AA) women have a higher frequency of BRCA1 mutations and TNBC compared to other populations. Basal-like tumors have a higher rate of brain, lung and distant nodal metastasis more than other TNBC subtypes, contributing to higher mortality rate. Our previous work suggested Ubc9, a SUMO E2-conjugating enzyme to induce proliferation and migration of BRCA1-incompetent TNBC cells and TNBC cell lines established from the pleural effusion metastasis of a woman with TNBC. To understand the downstream signaling axis involved in distant metastasis we have used clinically relevant BRCA1 mutant and lung metastatic TNBC cell lines and our results show deregulated expression of caveolin-1, VEGF and SIRT1 in these cells compared to normal mammary epithelial cells by immunofluorescence analysis. We observed SIRT1 to be induced by wild type BRCA1a and BRCA1a I26A mutant unlike the disease associated Ubc9 binding mutants in TNBC cells. Knock down of Ubc9 induced SIRT1 expression in TNBC and ER-α expression in breast cancer cells. This is the first report demonstrating a role for Ubc9 in repressing both SIRT1 and ER-α expression in BRCA1 associated TNBC cells. It also suggests that the BARD-dependent E3 Ubiquitin ligase and HR (homologous recombination) activity of BRCA1 may not be required for inducing SIRT1 expression. Our results suggest for the first time that in BRCA1 mutant TNBC Ubc9-mediated induction of VEGF, inhibition of caveolin-1, SIRT1 and ER-α expression as a novel molecular mechanism underlying TNBC EMT (epithelial mesenchymal transition) leading to lung metastasis with pleural effusion. Drugs that target Ubc9 to both induce SIRT1 and ER-α or using SIRT1 agonists in combination with chemotherapy can be used as a promising targeted therapeutic approach for treating basal-like metastatic BRCA1-linked TNBC thus reducing the mortality in patients with TNBC.","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"411 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77830757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium and Thyroid Health in NZ European Women","authors":"Ljiljana M Jowitt","doi":"10.15761/IMM.1000286","DOIUrl":"https://doi.org/10.15761/IMM.1000286","url":null,"abstract":"The primary aim of the study was to determine differences in the hormonal status (TSH, T3, and T4) between healthy participants and participants with Hashimoto’s thyroiditis. The secondary aim of the study was to assess plasma selenium nutritional status and its relationships with the serum levels of thyroid hormones in both of the groups. This study is a pilot and a cross-sectional. Study participants were assigned into two groups, the control group with healthy participants (n=13) and a group of women with Hashimoto’s thyroiditis (n=8). Any change in the participants’ daily routine was not required. For the measurement of plasma selenium, thyroid stimulating hormone, tetra iodothyronine, and triiodothyronine, creatinine, and glomerular filtration rate, participants’ non-fasting blood samples were taken. Two women with subclinical hypothyroidism were excluded from the statistical data analyses. Women with Hashimoto’s thyroiditis were older, had higher tetra iodothyronine level, low triiodothyronine/tetra iodothyronine ratio, higher plasma selenium levels, and similar triiodothyronine plasma levels to women in the control group. Significant relationships between triiodothyronine and selenium, triiodothyronine were identified in the group of women with Hashimoto’s thyroiditis. There was a moderate selenium deficiency in both of the groups that affected more women with hypothyroidism. Compromised peripheral deiodination in women with hypothyroidism required increased L-thyroxine dosing, which in turn increased the level of tetra iodothyronine, and decreased pituitary thyroid stimulating hormone, in order to achieve the desired level of the triiodothyronine. In order to increase plasma selenium level, recent research has suggested that selenium supplementation with selenomethionine or selenium selenite, might also slow down the process of thyroid destruction by thyroid autoantibodies in Hashimoto’s thyroiditis. In the past, the process of selenium supplementation had variable success rates. Therefore, further research is warranted. Abbreviations: TSH: Thyroid stimulating hormone; T3: Triiodothyronine; T4: Tetraiodothyronine; SeMet: Selenomethionine; SeCys: selenocysteine","PeriodicalId":94322,"journal":{"name":"Integrative molecular medicine","volume":"7 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80602990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}