Cancer Translational Medicine最新文献_第6页

The producing capabilities of Interferon-γ and Interleukin-10 of spleen cells in primary and metastasized oral squamous cell carcinoma cells-implanted mice 原发性和转移性口腔鳞状细胞癌移植小鼠脾细胞产生干扰素-γ和白细胞介素-10的能力

Cancer Translational Medicine Pub Date : 2017-11-01 DOI: 10.4103/ctm.ctm_30_17

Yasuka Azuma, M. Mizuno-Kamiya, E. Takayama, Harumi Kawaki, T. Inagaki, E. Chihara, Y. Muramatsu, N. Kondoh

{"title":"The producing capabilities of Interferon-γ and Interleukin-10 of spleen cells in primary and metastasized oral squamous cell carcinoma cells-implanted mice","authors":"Yasuka Azuma, M. Mizuno-Kamiya, E. Takayama, Harumi Kawaki, T. Inagaki, E. Chihara, Y. Muramatsu, N. Kondoh","doi":"10.4103/ctm.ctm_30_17","DOIUrl":"https://doi.org/10.4103/ctm.ctm_30_17","url":null,"abstract":"Aim: The aim of this study is to compare the immunomodulatory effects exerted by primary versus metastasized oral squamous cell carcinoma (OSCC) cells. Methods: Mouse OSCC cell line Sq-1979, 233 cells established from implanted Sq-1979 cells, and L cells established from the metastasized lymph node tissues of Sq-1979-implanted mice were subcutaneously inoculated into the later abdominal area of syngeneic C3H mice. The producing capabilities of interferon (IFN)-γ, a Th1 cytokine, and interleukin (IL)-10, a Th2 cytokine, by anti-CD3 antibody-stimulated spleen cells were investigated in tumor bearing-mice. Results: The quantity of IFN-γ produced by stimulated spleen cells was significantly suppressed in the mice implanted with Sq-1979, 233, and L cells. Conversely, the production of IL-10 was significantly elevated in Sq-1979 and 233 cell-implanted mice while markedly suppressed in L cell-implanted mice. Conclusion: Our results suggest that the metastasized L cells have acquired differential immunomodulatory functions compared to the original Sq-1979 and primary 233 cells.","PeriodicalId":9428,"journal":{"name":"Cancer Translational Medicine","volume":"25 1","pages":"194 - 199"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73464924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Biomarkers in molecular epidemiology study of oral squamous cell carcinoma in the era of precision medicine 精准医学时代口腔鳞状细胞癌分子流行病学研究中的生物标志物

Cancer Translational Medicine Pub Date : 2017-11-01 DOI: 10.4103/ctm.ctm_32_17

Qingshi Zhu, Qingyao Shang, Zhi-hao Hu, Yuan Liu, Bo Li, Bo Wang, An-hui Wang

{"title":"Biomarkers in molecular epidemiology study of oral squamous cell carcinoma in the era of precision medicine","authors":"Qingshi Zhu, Qingyao Shang, Zhi-hao Hu, Yuan Liu, Bo Li, Bo Wang, An-hui Wang","doi":"10.4103/ctm.ctm_32_17","DOIUrl":"https://doi.org/10.4103/ctm.ctm_32_17","url":null,"abstract":"Oral cancer, which occurs in the mouth, lips, and tongue, is a multifactorial disease whose etiology involves environment, genetic, and epigenetic factors. Tobacco use and alcohol consumption are regarded as the primary risk factors for oral squamous cell carcinoma (OSCC), and betel use, other chemicals, radiation, environmental, and genetics are reported as relevant risk factors for oral carcinogenesis. The human papillomavirus infection is an independent risk factor. Traditional epidemiology studies have revealed that environmental carcinogens are risk factors for OSCC. Molecular epidemiology studies have revealed that the susceptibility to OSCC is influenced by both environmental and genetic risk factors. However, the details and mechanisms of risk factors involved in OSCC are unclear. Advanced methods and techniques used in human genome studies provide great opportunities for researchers to explore and identify (a) the details of such risk factors and (b) genetic susceptibility involved in OSCC. Human genome epidemiology is a new branch of epidemiology, which leads the epidemiology study from the molecular epidemiology era into the era of genome-wide association study. In the era of precision medicine, molecular epidemiology studies should focus on biomarkers for cancer genomics and their potential utility in clinical practice. Here, we briefly reviewed several molecular epidemiology studies of OSCC, focusing on biomarkers as valuable utility in risk assessment, clinical screening, diagnosis, and prognosis prediction of OSCC in the era of precision medicine.","PeriodicalId":9428,"journal":{"name":"Cancer Translational Medicine","volume":"11 1","pages":"214 - 218"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79716728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

“Eating” Cancer cells by blocking CD47 signaling: Cancer therapy by targeting the innate immune checkpoint 通过阻断CD47信号传导“吃掉”癌细胞:靶向先天免疫检查点的癌症治疗

Cancer Translational Medicine Pub Date : 2017-11-01 DOI: 10.4103/ctm.ctm_26_17

Y. Xiang, Li Liu

引用次数: 14

Glycosylation is involved in malignant properties of cancer cells 糖基化与癌细胞的恶性特性有关

Cancer Translational Medicine Pub Date : 2017-11-01 DOI: 10.4103/ctm.ctm_28_17

K. Hamamura, K. Furukawa

引用次数: 3

The significance of nuclear factor-kappa B signaling pathway in glioma: A review 核因子- κ B信号通路在胶质瘤中的意义

Cancer Translational Medicine Pub Date : 2017-09-01 DOI: 10.4103/ctm.ctm_48_16

Xiaoshan Xu, Hongwei Yang, Xin Wang, Y. Tu

引用次数: 3

Motion estimation of the liver based on deformable image registration: a comparison between four-dimensional-computed tomography and four-dimensional-magnetic resonance imaging 基于可变形图像配准的肝脏运动估计:四维计算机断层扫描和四维磁共振成像的比较

Cancer Translational Medicine Pub Date : 2017-09-01 DOI: 10.4103/ctm.ctm_24_17

X. Liang, F. Yin, Yilin Liu, B. Czito, M. Palta, M. Bashir, Jing Cai

{"title":"Motion estimation of the liver based on deformable image registration: a comparison between four-dimensional-computed tomography and four-dimensional-magnetic resonance imaging","authors":"X. Liang, F. Yin, Yilin Liu, B. Czito, M. Palta, M. Bashir, Jing Cai","doi":"10.4103/ctm.ctm_24_17","DOIUrl":"https://doi.org/10.4103/ctm.ctm_24_17","url":null,"abstract":"Aim: The aim of this study was to evaluate deformable image registration (DIR)-based motion estimation of the liver for four-dimensional-computed tomography (4D-CT) and 4D-magnetic resonance imaging (MRI). Methods: Five liver cancer patients were included. Each patient was imaged with 4D-CT and 4D-MRI under an Institutional Review Board-approved protocol. Motion estimation of the liver was obtained by performing DIR on 4D-CT and 4D-MRI. A region of interest (ROI) encompassing the expert-determined gross tumor volume was used as surrogate to evaluate the accuracy of the motion estimation. ROI motion trajectories were estimated by averaging the displacement vector fields (DVFs) within the ROI during the breathing cycles for 4D-CT and 4D-MRI and were compared to those extracted from cine MR. Target registration error (TRE), correlation coefficient (CC) for phase agreement, difference in phase at maximum displacement (ΔPmax), and Dice's Similarity Coefficient (DSC) for overall motion agreement were determined. Results: As compared to 4D-CT, 4D-MRI resulted in smaller TRE in DVFs (anterior-posterior [AP]: 1.0 ± 0.4 mm vs. 1.5 ± 0.5 mm, superior-inferior [SI]: 1.9 ± 0.7 mm vs. 2.2 ± 0.8 mm), greater CC (AP: 0.67 ± 0.32 vs. 0.49 ± 0.26, SI: 0.84 ± 0.15 vs. 0.58 ± 0.28), smaller ΔPmax (AP: 1.4 ± 1.7 vs. 2.0 ± 1.0, SI: 0.4 ± 0.9 vs. 1.2 ± 0.8), and greater DSC (AP: 0.67 ± 0.08 vs. 0.61 ± 0.11, SI: 0.73 ± 0.12 vs. 0.67 ± 0.10). Conclusion: 4D-MRI can potentially provide more realistic respiratory DVFs of the liver than 4D-CT.","PeriodicalId":9428,"journal":{"name":"Cancer Translational Medicine","volume":"8 1","pages":"153 - 158"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84411894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Recent progress in technological improvement and biomedical applications of the clustered regularly interspaced short palindromic repeats/cas system 聚类规则间隔短回文重复序列/cas系统的技术改进及生物医学应用进展

Cancer Translational Medicine Pub Date : 2017-09-01 DOI: 10.4103/ctm.ctm_22_17

Yanlan Li, Zheng Hu, Yufang Yin, Rongzhang He, Jian Hu, Weihao Luo, Jia Li, Gebo Wen, Li Xiao, Kai Li, Duan-fang Liao, Dixian Luo

引用次数: 0

A feasibility study of applying thermal imaging to assist quality assurance of high-dose rate brachytherapy 应用热成像辅助高剂量率近距离放射治疗质量保证的可行性研究

Cancer Translational Medicine Pub Date : 2017-09-01 DOI: 10.4103/ctm.ctm_25_17

Xiaofeng Zhu, Y. Lei, D. Zheng, Sicong Li, V. Verma, Mutian Zhang, Qinghui Zhang, Xiaoli Tang, J. Lian, Sha X. Chang, Haijun Song, Sumin Zhou, C. Enke

{"title":"A feasibility study of applying thermal imaging to assist quality assurance of high-dose rate brachytherapy","authors":"Xiaofeng Zhu, Y. Lei, D. Zheng, Sicong Li, V. Verma, Mutian Zhang, Qinghui Zhang, Xiaoli Tang, J. Lian, Sha X. Chang, Haijun Song, Sumin Zhou, C. Enke","doi":"10.4103/ctm.ctm_25_17","DOIUrl":"https://doi.org/10.4103/ctm.ctm_25_17","url":null,"abstract":"Aim: High-dose rate (HDR) brachytherapy poses a special challenge to radiation safety and quality assurance (QA) due to its high radioactivity, and it is thus critical to verify the HDR source location and its radioactive strength. This study explores a new application for thermal imaging, to visualize/locate the HDR source and measure radioactivity using temperature information. A potential application would relate to HDR QA and safety improvement. Methods: Heating effects by an HDR source were studied using finite element analysis (FEA). Thermal cameras were used to visualize an HDR source inside a plastic catheter made of polyvinylidene difluoride (PVDF). Using different source dwell times, relationships between the HDR source strength and heating effects were studied, thus establishing potential daily QA criteria using thermal imaging. Results: For an Ir-192 source with a source radioactivity of 10 Ci, the decay-induced heating power inside the source was about 13.3 mW. After the HDR source was extended into the PVDF applicator and reached thermal equilibrium, thermal imaging visualized the temperature gradient of 10 K/cm along the PVDF catheter surface, which agreed with FEA modeling. For the Ir-192 source strengths ranging from 16.9 to 41.1 kU, thermal imaging could verify source activity with a relative error of 6.3% with a dwell time of 10 s, and a relative error of 2.5% with 100 s. Conclusion: Thermal imaging could be a feasible tool to visualize HDR source dwell positions and verify source integrity. Potentially, patient safety and treatment quality may be improved by integrating thermal measurements into HDR QA procedures.","PeriodicalId":9428,"journal":{"name":"Cancer Translational Medicine","volume":"51 1","pages":"159 - 166"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89216793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Combination of Interleukin-11Rα chimeric antigen receptor T-cells and programmed death-1 blockade as an approach to targeting osteosarcoma cells In vitro 白细胞介素- 11r α嵌合抗原受体t细胞联合程序性死亡-1阻断作为体外靶向骨肉瘤细胞的方法

Cancer Translational Medicine Pub Date : 2017-07-01 DOI: 10.4103/ctm.ctm_3_17

H. Moonat, Gangxiong Huang, P. Dhupkar, Keri L Schadler, N. Gordon, E. Kleinerman

{"title":"Combination of Interleukin-11Rα chimeric antigen receptor T-cells and programmed death-1 blockade as an approach to targeting osteosarcoma cells In vitro","authors":"H. Moonat, Gangxiong Huang, P. Dhupkar, Keri L Schadler, N. Gordon, E. Kleinerman","doi":"10.4103/ctm.ctm_3_17","DOIUrl":"https://doi.org/10.4103/ctm.ctm_3_17","url":null,"abstract":"Aim: To test whether combining interleukin (IL)-11Rα chimeric antigen receptor (CAR) T-cells with an anti-programmed death (PD-1) antibody (an immune checkpoint inhibitor) is an effective therapeutic approach in osteosarcoma (OS), allowing improved tumor eradication. Methods: IL-11Rα-CAR T-cells were cocultured in vitro with human LM7 OS tumor cells (with and without anti-PD-1 antibody). Coculture of LM7 cells with purified T-cells served as the control. Cytotoxicity and surface PD-1 expression were analyzed in all groups. Results: PD-1 expression increased during expansion of CAR T-cells. Exposure of immune cells to tumor cells in vitro subsequently decreased surface PD-1 expression on the CAR T-cells. Addition of an anti-PD-1 antibody (Clone J110) to further decrease surface PD-1 expression on CAR T-cells before coculture did not enhance cytotoxic effects of the CAR T-cells against LM7 cells. Conclusion: This combination of IL-11Rα-CAR T-cells and an anti-PD-1 antibody did not provide any additional cytotoxic benefit over IL-11Rα-CAR T-cell therapy alone in this setting. Further studies are needed as simple interference with surface PD-1 expression alone may not be sufficient to inhibit this immune checkpoint pathway to then enhance IL-11Rα-CAR T-cell therapeutic effects.","PeriodicalId":9428,"journal":{"name":"Cancer Translational Medicine","volume":"24 1","pages":"139 - 145"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90317605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A T-cell engager-armed oncolytic vaccinia virus to target the tumor stroma 一种靶向肿瘤基质的t细胞接合子溶瘤痘苗病毒

Cancer Translational Medicine Pub Date : 2017-07-01 DOI: 10.4103/ctm.ctm_13_17

Feng Yu, Bangxing Hong, Xiao-tong Song

{"title":"A T-cell engager-armed oncolytic vaccinia virus to target the tumor stroma","authors":"Feng Yu, Bangxing Hong, Xiao-tong Song","doi":"10.4103/ctm.ctm_13_17","DOIUrl":"https://doi.org/10.4103/ctm.ctm_13_17","url":null,"abstract":"Aim: Cancer-associated fibroblasts (CAFs) are the key cellular components of the tumor stroma. CAFs express fibroblast activation protein (FAP) and FAP-targeted immunotherapies have shown potent antitumor effects in preclinical mouse studies, highlighting their central role in tumorigenesis. However, safety concerns have been raised in regard to FAP-targeted immunotherapies since bone marrow failure and cachexia were observed in transgenic models and preclinical studies. Here, we describe a novel oncolytic virotherapy by locally targeting FAP within tumor tissue. Methods: T-cell engager-armed oncolytic vaccinia virus (TEA-VV) that encodes a secretory bi-specific T-cell engager consisting of two single-chain variable fragments specific for murine CD3 and fibroblast activation protein (mFAP-TEA-VV) was generated. The antitumor effects of mFAP-TEA-VV were compared to unmodified VVs using standard in vitro immunological assays and an immunocompetent B16 melanoma mouse model. Results: In vitro, the ability of mFAP-TEA-VV to replicate within tumor cells and induce oncolysis was similar to that of unmodified VVs. However, in co-culture assays, only mFAP-TEA-VV induced bystander killing of noninfected FAP-expressing cells in the presence of murine T-cells. In vivo, mFAP-TEA-VV enhanced viral titer within the tumor and had potent antitumor activity in comparison to control VVs in an immunocompetent B16 melanoma mouse model. Importantly, the improved viral spread of mFAP-TEA-VV correlated with the destruction of tumor stroma. Conclusion: Arming oncolytic VVs with an FAP-targeted T-cell engager may be a promising improvement to oncolytic virus therapy for solid tumors.","PeriodicalId":9428,"journal":{"name":"Cancer Translational Medicine","volume":"45 1","pages":"122 - 132"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91528989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25