Virology最新文献_第2页

Expression and purification of PNGase F protein in yeast and its anti-PRV activity. PNGase F蛋白在酵母中的表达纯化及其抗prv活性研究。

Virology Pub Date : 2025-01-15 DOI: 10.1016/j.virol.2025.110393

Haonan Zhang, Yu Jiang, Gang Ding, Jingyu Chen, Yuda Liu, Furong Wang, Xiaolan Yu

{"title":"Expression and purification of PNGase F protein in yeast and its anti-PRV activity.","authors":"Haonan Zhang, Yu Jiang, Gang Ding, Jingyu Chen, Yuda Liu, Furong Wang, Xiaolan Yu","doi":"10.1016/j.virol.2025.110393","DOIUrl":"https://doi.org/10.1016/j.virol.2025.110393","url":null,"abstract":"Pseudorabies virus (Pseudorabiesvirus, PRV) has caused huge economic losses to the global pig industry. In recent years, it has been reported that there are PRV mutants, but the traditional vaccine can not completely prevent or control the infection of PRV, so there is an urgent need to develop new broad-spectrum anti-disease drugs for prevention and treatment. PNGase F from bacteria can catalyze the hydrolysis of oligosaccharides linked to asparagine residues on peptides, so we speculate that PNGase F can inhibit virus infection by removing the glycosylation of virus membrane glycoproteins. In this study, PNGase F protein was highly expressed and purified in Pichia pastoris, and the deglycosylation activity of PNGase F expressed in Pichia pastoris was verified. In vitro, 15 μM could significantly inhibit the proliferation of virus in cells. The results of cytotoxicity test showed that PNGase F was not toxic to many cells. To further evaluate the effect of PNGase F in different stages of virus infection, it was found that PNGase F had significant inhibitory effect on virus adsorption and invasion. In vivo experiments in mice, PNGase F could significantly inhibit the replication of PRV Ea strain in mice and inhibit PRV, reduced brain lesions. Our experiments show that PNGase F expressed by yeast can inhibit PRV infection in vitro and in vitro, and its inhibitory mechanism is preliminarily discussed, which can provide a new reference for the development of broad-spectrum antiviral drugs based on PNGase F.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110393"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Segment-specific promoter activity for RNA synthesis in the genome of Oz virus, genus Thogotovirus. Oz病毒基因组中RNA合成的片段特异性启动子活性。

Virology Pub Date : 2025-01-15 DOI: 10.1016/j.virol.2025.110410

Lipi Akter, Ryo Matsumura, Daisuke Kobayashi, Hiromichi Matsugo, Haruhiko Isawa, Yusuke Matsumoto

引用次数: 0

Enteric pathogenicity characterization of emerging parainfluenza virus 5 in western China.

Virology Pub Date : 2025-01-15 DOI: 10.1016/j.virol.2025.110409

Minting Ni, Shengyu Lin, Yongheng Shao, Jiao Tang, Shuxian Li, Chen Tan, Zhenli Gong, Hongbo Li, Jintao Wang, Guangliang Liu, Jianing Chen

引用次数: 0

An effective antiviral strategy based on silence of susceptibility genes through cucumber mosaic virus (CMV) attenuated vaccine vector. 黄瓜花叶病毒（CMV）减毒疫苗载体中易感基因沉默的有效抗病毒策略

Virology Pub Date : 2025-01-11 DOI: 10.1016/j.virol.2025.110396

Naihe Wang, Cheng Yuan, Zhao Wang, Chengming Yu, Zhifei Liu, Shuyuan Tian, Kaiqiang Hao, Xuefeng Yuan

{"title":"An effective antiviral strategy based on silence of susceptibility genes through cucumber mosaic virus (CMV) attenuated vaccine vector.","authors":"Naihe Wang, Cheng Yuan, Zhao Wang, Chengming Yu, Zhifei Liu, Shuyuan Tian, Kaiqiang Hao, Xuefeng Yuan","doi":"10.1016/j.virol.2025.110396","DOIUrl":"https://doi.org/10.1016/j.virol.2025.110396","url":null,"abstract":"Plant viruses represent a major threat to agriculture, affecting a wide range of crops with substantial economic losses. This study presented a novel strategy for managing plant viral diseases through the development an attenuated vaccine utilizing cucumber mosaic virus (CMV) for virus-induced gene silencing (VIGS) targeting susceptibility gene. TOBAMOVIRUS MULTIPLICATION 2A (TOM2A) gene was identified as a critical factor that enhances susceptibility to TMV infection in plants. Two vaccines were constructed based on CMV attenuated vaccine vector-CR2V. The first vaccine was constructed by incorporating NtTOM2A fragments into CR2V, while the second vaccine was developed by inserting both NtTOM2A fragments and TMV-p183 fragments into CR2V. Each vaccine constructs significantly reduced TMV accumulation, exhibited no adverse effects on plant growth, and maintained stability of the inserted sequences up to 21 days post-vaccination (dpv) in vivo. The study underscored the potential of utilizing engineered plant viruses as environmentally sustainable vaccines for mitigating viral diseases in agriculture.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110396"},"PeriodicalIF":0.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibody responses in omicron BF.7-infected patients vaccinated with inactivated SARS-CoV-2. 灭活SARS-CoV-2疫苗接种组粒bf .7感染患者的抗体应答

Virology Pub Date : 2025-01-10 DOI: 10.1016/j.virol.2025.110404

Yabo Zhao, Weili Li, Liming Xu, Zhanling Tao, Junhe Zhang

{"title":"Antibody responses in omicron BF.7-infected patients vaccinated with inactivated SARS-CoV-2.","authors":"Yabo Zhao, Weili Li, Liming Xu, Zhanling Tao, Junhe Zhang","doi":"10.1016/j.virol.2025.110404","DOIUrl":"https://doi.org/10.1016/j.virol.2025.110404","url":null,"abstract":"Objective: Our study aimed to investigate antibody responses in omicron BF.7-infected patients after being vaccinated with inactivated SARS-CoV-2.Methods: Blood serum samples were collected every 2-7 d, 1 w before infection, during the acute infection period and recovery period, and every month after recovery to detect IgG, IgM, IgA, neutralizing antibodies, and neutralizing antibodies against different omicrons in the acute phase.Results: The levels of IgG, IgA and neutralizing antibodies increased sharply at 6-7 d after infection, and the levels of IgG and neutralizing antibodies peaked rapidly within 1-2 w, lasting for 3-4 w, and the antibody levels gradually decreased at 4-8 w after infection. The level of neutralizing antibodies against the wild-type strain SARS-CoV-2 was much greater than that against the variant strains during the acute infection period.Conclusion: After being infected with omicron BF.7, the IgG and neutralizing antibodies in inactivated SARS-CoV-2-vaccinated individuals increase sharply 6-7 days after infection, which is earlier than those in the initial WT SARS-CoV-2 infection, and the peak time within 1-2 w is shorter. The levels of IgG, neutralizing antibody, and IgA antibodies are high, and the levels of IgM antibodies are low; however, the neutralizing antibodies are mainly against the wild-type strain of SARS-CoV-2.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110404"},"PeriodicalIF":0.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a cell-adapted completely attenuated genotype GIIa porcine epidemic diarrhea virus strain.

Virology Pub Date : 2025-01-10 DOI: 10.1016/j.virol.2025.110407

Ruiming Yu, Liping Zhang, Dongsheng Wang, Jun Yang, Peng Zhou, Yuhan Wen, Mingxia Li, Yingjie Bai, Zhongwang Zhang, Yousheng Peng, Yanzhen Lu, Dan Li, Jian He, Yonglu Wang, Huichen Guo, Li Pan, Xinsheng Liu

{"title":"Characterization of a cell-adapted completely attenuated genotype GIIa porcine epidemic diarrhea virus strain.","authors":"Ruiming Yu, Liping Zhang, Dongsheng Wang, Jun Yang, Peng Zhou, Yuhan Wen, Mingxia Li, Yingjie Bai, Zhongwang Zhang, Yousheng Peng, Yanzhen Lu, Dan Li, Jian He, Yonglu Wang, Huichen Guo, Li Pan, Xinsheng Liu","doi":"10.1016/j.virol.2025.110407","DOIUrl":"https://doi.org/10.1016/j.virol.2025.110407","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV) has caused significant harm to the global pig industry since its discovery. In this study, a highly pathogenic strain of GIIa PEDV CH/HBXT/2018, isolated previously, was continuously passaged in Vero cells up to passage (P)240, resulting in a completely attenuated virus. The proliferation characteristics of different passages of the strain in Vero cells, pathogenicity in newborn piglets, and mutations in S gene sequence indicated that as the passage number increased, the replication efficiency of PEDV in Vero cells gradually improved, with a more pronounced cytopathic effect. However, its pathogenicity in piglets decreased progressively, evident as reduced viral loads in the feces and intestinal tissues, less-severe clinical symptoms, less-severe histopathological damage, and lower antigen expression in intestinal tissues. At P240, the strain was completely attenuated. A sequence analysis revealed 17 amino acid mutations in the structural spike protein, which may have contributed to the biological changes observed at P240. Furthermore, compared with P10, the strain's dependence on trypsin had decreased significantly at P200. A differential transcriptomic analysis revealed 1712 differentially expressed genes (DEGs) between the P10 and P200 infection groups, of which 458 were upregulated and 1254 downregulated. These DEGs were primarily involved in signaling pathways such as cytokine-cytokine receptor interaction, inflammatory response, and MHC protein complex. Our findings provide valuable insights into the mechanisms of PEDV attenuation and should facilitate the development of live vaccines.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"604 ","pages":"110407"},"PeriodicalIF":0.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bat coronavirus surveillance across different habitats in Yucatán, México. 牙买加Yucatán不同栖息地的蝙蝠冠状病毒监测。

Virology Pub Date : 2025-01-10 DOI: 10.1016/j.virol.2025.110401

Marco Antonio Jiménez-Rico, Ana Laura Vigueras-Galván, Erika N Hernández-Villegas, Paola Martínez-Duque, David Roiz, Luisa I Falcón, Ella Vázquez-Domínguez, Osiris Gaona, Audrey Arnal, Benjamin Roche, Rosa Elena Sarmiento-Silva, Gerardo Suzán

{"title":"Bat coronavirus surveillance across different habitats in Yucatán, México.","authors":"Marco Antonio Jiménez-Rico, Ana Laura Vigueras-Galván, Erika N Hernández-Villegas, Paola Martínez-Duque, David Roiz, Luisa I Falcón, Ella Vázquez-Domínguez, Osiris Gaona, Audrey Arnal, Benjamin Roche, Rosa Elena Sarmiento-Silva, Gerardo Suzán","doi":"10.1016/j.virol.2025.110401","DOIUrl":"https://doi.org/10.1016/j.virol.2025.110401","url":null,"abstract":"Bats, which play a vital role in maintaining ecosystems, are also known as natural reservoirs of coronaviruses (CoVs), thus have raised concerns about their potential transmission to humans, particularly in light of the emergence of MERS-CoV, SARS-CoV, and SARS-CoV-2. The increasing impact of human activities and ecosystem modifications is reshaping bat community structure and ecology, heightening the risk of the emergence of potential epidemics. Therefore, continuous monitoring of these viruses in bats is necessary. Despite the rich diversity of bats species in México, few studies have been conducted to search for CoVs in these group of mammals. In the present study we conducted CoV surveillance across previously unexplored sites in the state of Yucatán, a state located within the ecologically diverse Yucatán Peninsula, a tropical region undergoing pronounced anthropogenic changes, including deforestation, agricultural expansion and urbanization. We captured 191 bats between 2021 and 2022 in three different habitats: diversified rural (Tzucacab), rural (Tizimín) and urban (Mérida). Molecular analyses had revealed a 5.4% CoV prevalence, with the diversified rural site exhibiting a notably elevated rate (26.3%). Subsequent sequencing and phylogenetic assessment revealed four distinct Alphacoronavirus genotypes, indicating host-specific clustering among Phyllostomidae bats. Notably, one was detected for the first time in a Sturnira species. Our findings suggest a reduced likelihood of transmission of these viruses to humans or other species, evidenced by clustering patterns and sequence dissimilarity with known CoVs. We emphasize that maintaining sustained virus surveillance in bats is crucial to understanding viral diversity and identifying potential risks to human and animal health.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110401"},"PeriodicalIF":0.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization, phylogeny and prevalence of the coding-complete genomes of aviadenoviruses and siadenovirus in young racing pigeons. 雏赛鸽中鸟腺病毒和腺病毒编码全基因组的特征、系统发育和流行。

Virology Pub Date : 2025-01-09 DOI: 10.1016/j.virol.2025.110400

Ewa Łukaszuk, Daria Dziewulska, Agata Prątnicka, Joy M Custer, Simona Kraberger, Arvind Varsani, Tomasz Stenzel

{"title":"Characterization, phylogeny and prevalence of the coding-complete genomes of aviadenoviruses and siadenovirus in young racing pigeons.","authors":"Ewa Łukaszuk, Daria Dziewulska, Agata Prątnicka, Joy M Custer, Simona Kraberger, Arvind Varsani, Tomasz Stenzel","doi":"10.1016/j.virol.2025.110400","DOIUrl":"https://doi.org/10.1016/j.virol.2025.110400","url":null,"abstract":"Adenoviruses are a concern for pigeon breeders due to their impact on animal health. Furthermore, they have been studied for nearly five decades and are one of the most studied viruses in pigeons. However, the number of complete genomic sequences of pigeon-infecting adenoviruses available is very low, and the pathogenic effect of these viruses on pigeons is still yet to be thoroughly explored. In this study, we describe the usage of high-throughput sequencing to identify and assemble genomic sequences of adenoviruses from pigeon samples. We were able to recover two coding-complete genomic sequences of members of Aviadenovirus genus, presumably belonging to Aviadenovirus columbidae species, and the first coding-complete genomic sequence of a member of Siadenovirus genus derived from a pigeon, genetically distinct from all other known species in this genus, which we proposed to be named Siadenovirus columbae. In addition, we designed quantitative PCR assay with TaqMan probes and digital droplet PCR to examine the potential link between the amount of pigeon adenovirus 2 (species Aviadenovirus columbidae) genome copies and the occurrence of enteropathy in young racing pigeons. The results of the quantitative analysis show that pigeon adenovirus 2 occurs in both healthy and diseased pigeons which is consistent with the previous findings. Further research is needed to assess how adenoviruses may influence enteric disease in racing pigeons.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110400"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review of impaired immune parameters in RSV infections in the elderly. 老年人呼吸道合胞病毒感染中免疫参数受损的综述。

Virology Pub Date : 2025-01-08 DOI: 10.1016/j.virol.2025.110395

Jonathan L Miller, Stefan Niewiesk

引用次数: 0

Characterization of a highly pathogenic porcine Teschoviruses 5 emerged in Western China. 中国西部出现的猪高致病性teschovirus 5的特征分析。

Virology Pub Date : 2025-01-07 DOI: 10.1016/j.virol.2025.110398

Yongheng Shao, Yabin Lu, Shuxian Li, Shengyu Lin, Jiao Tang, Chen Tan, Zhenli Gong, Wenhui Wang, Guangliang Liu, Jianing Chen

{"title":"Characterization of a highly pathogenic porcine Teschoviruses 5 emerged in Western China.","authors":"Yongheng Shao, Yabin Lu, Shuxian Li, Shengyu Lin, Jiao Tang, Chen Tan, Zhenli Gong, Wenhui Wang, Guangliang Liu, Jianing Chen","doi":"10.1016/j.virol.2025.110398","DOIUrl":"https://doi.org/10.1016/j.virol.2025.110398","url":null,"abstract":"Porcine teschovirus (PTV) is a devastating virus that targets the central nervous system and led to great economic losses in Europe between the 1920s and 1960s. Since 1973, PTV variants with lower pathogenicity have been prevalent globally, whereas highly pathogenic PTV strains have rarely emerged. In 2022, diarrhea with high mortality occurred on a pig farm in Gansu China. Virome analysis revealed that PTV was enriched among diarrheal samples. A PTV strain was then isolated and characterized by TEM, IFA, and growth kinetic features. Phylogenetic analyses revealed that the isolate shared an identity of approximately 90% with the most related PTV strain and with various mutant clusters among VP1. Further animal tests demonstrated that the isolate can result in serious respiratory distress, watery diarrhea, paralysis and high mortality in challenged pigs. H&E staining revealed the presence of lymphocyte cells infiltration and hemorrhage in the tissues. Overall, a PTV variant with high mortality was identified in western China, which could result in interstitial pneumonia, hemorrhage, and diarrhea. Although most PTV strains are associated with asymptomatic infection now, the sporadic occurrence of highly pathogenic PTVs is worthy of alarm.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110398"},"PeriodicalIF":0.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0