Translational research : the journal of laboratory and clinical medicine最新文献

Corrigendum to "Early vascular aging in chronic kidney disease: focus on microvascular maintenance, senescence signature and potential therapeutics" [Translational Research 275 (2025) 32-47]. “慢性肾脏疾病的早期血管衰老：关注微血管维持、衰老特征和潜在治疗方法”[Translational Research] 275(2025) 32-47]。

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-02-01 Epub Date: 2024-12-21 DOI: 10.1016/j.trsl.2024.12.003

Samsul Arefin, Neja Mudrovcic, Sam Hobson, Federico Pietrocola, Thomas Ebert, Liam J Ward, Anna Witasp, Leah Hernandez, Lars Wennberg, Torbjörn Lundgren, Julia Steinmetz-Späh, Karin Larsson, Anders Thorell, Stefania Bruno, Marita Marengo, Vincenzo Cantaluppi, Peter Stenvinkel, Karolina Kublickiene

引用次数: 0

Infectious agents in the pathogenesis of autoimmune rheumatic diseases. 自身免疫性风湿病发病机制中的传染因子。

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-02-01 Epub Date: 2024-12-30 DOI: 10.1016/j.trsl.2024.12.004

Aleksandra Korzeniowska, Ewa Bryl

引用次数: 0

Dissecting the cellular reprogramming and tumor microenvironment in left- and right-sided Colorectal Cancer by single cell RNA sequencing. 通过单细胞 RNA 测序剖析左右侧结直肠癌的细胞重编程和肿瘤微环境

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-02-01 Epub Date: 2024-12-13 DOI: 10.1016/j.trsl.2024.12.002

Congxue Hu, Xiaozhi Huang, Jing Chen, Weixin Liang, Kaiyue Yang, Hui Jiang, Kuan Yang, Qi Ou, Xia Li, Yunpeng Zhang

{"title":"Dissecting the cellular reprogramming and tumor microenvironment in left- and right-sided Colorectal Cancer by single cell RNA sequencing.","authors":"Congxue Hu, Xiaozhi Huang, Jing Chen, Weixin Liang, Kaiyue Yang, Hui Jiang, Kuan Yang, Qi Ou, Xia Li, Yunpeng Zhang","doi":"10.1016/j.trsl.2024.12.002","DOIUrl":"10.1016/j.trsl.2024.12.002","url":null,"abstract":"Sidedness and staging are major sources of tumor microenvironment (TME) differences in colorectal cancer (CRC). Subpopulation compositions of stromal cells and immune cells, and interactions between cells collectively constitute the immunosuppressive microenvironment of CRC. In this study, we comprehensively collected single-cell RNA sequencing data from public databases. We filtered out 126,279 cells from 55 CRC samples to characterize the differences in cellular composition, and to elucidate the transcriptional features and potential functions of cell types, temporally and positionally. We observed an increased degree of hypoxia in right side-specific cancer cells compared to left-sided cancer. Cancer-associated fibroblasts (CAFs) illustrated molecular signatures tremendously tended to be associated with functions that orchestrate extracellular matrix remodeling and angiogenesis, and right-sided CAFs characterized the stronger cancer invasion signals. Crosstalk between side-specific cancer cells and stromal together with immune cells characterized CRC via different sample groups, and was pertinent to worse prognosis. Our study captured immunosuppressive pattern exhibiting more intricate intercellular interactions in right-sided CRC. Additionally, during malignant progression of CRC, the transformation of CD8+ T cell cytotoxic and exhausted properties and macrophage pro-inflammatory and anti-inflammatory properties epitomized the cellular reprogramming phenomenon that the function of TME shifted from promoting immunity to suppressive immunity. Our study shed lights on refining personalized therapeutic regimens during malignant progression in left- and right-sided CRCs.","PeriodicalId":94257,"journal":{"name":"Translational research : the journal of laboratory and clinical medicine","volume":" ","pages":"22-37"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Tumor molecular landscape of Epstein-Barr virus (EBV) related nasopharyngeal carcinoma in EBV-endemic and non-endemic areas: Implications for improving treatment modalities" [Transl. Res. 265 (2024) 1-16]. eb病毒（EBV）相关鼻咽癌在eb病毒流行地区和非流行地区的肿瘤分子格局：改善治疗方式的意义[译]。Res. 265(2024) 1-16]。

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-02-01 Epub Date: 2024-12-16 DOI: 10.1016/j.trsl.2024.12.001

Deborah Lenoci, Carlo Resteghini, Mara S Serafini, Federico Pistore, Silvana Canevari, Brigette Ma, Stefano Cavalieri, Salvatore Alfieri, Annalisa Trama, Lisa Licitra, Loris De Cecco

引用次数: 0

Breaking new ground: Unraveling the USP1/ID3/E12/P21 axis in vascular calcification. 开辟新天地：揭示血管钙化中的 USP1/ID3/E12/P21 轴。

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-02-01 Epub Date: 2024-09-24 DOI: 10.1016/j.trsl.2024.09.002

Aoran Huang, Jianyun Rao, Xin Feng, Xingru Li, Tianhua Xu, Li Yao

引用次数: 0

Cardiopulmonary bypass with deep hypothermic circulatory arrest results in organ-specific transcriptomic responses in pediatric swine. 在儿科猪中，体外循环与深度低温循环停止导致器官特异性转录组反应。

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-01-17 DOI: 10.1016/j.trsl.2025.01.002

Jesse A Davidson, John Iguidbashian, Ludmila Khailova, Tanner Lehmann, Alejandro Suarez-Pierre, Lindsay M Thomson, Jack Zakrzewski, Eiman Ali, Schuyler Lee, Benjamin S Frank, Richard J Ing, Matthew L Stone, Suzanne Osorio Lujan, Sierra Niemiec, Christopher A Mancuso

{"title":"Cardiopulmonary bypass with deep hypothermic circulatory arrest results in organ-specific transcriptomic responses in pediatric swine.","authors":"Jesse A Davidson, John Iguidbashian, Ludmila Khailova, Tanner Lehmann, Alejandro Suarez-Pierre, Lindsay M Thomson, Jack Zakrzewski, Eiman Ali, Schuyler Lee, Benjamin S Frank, Richard J Ing, Matthew L Stone, Suzanne Osorio Lujan, Sierra Niemiec, Christopher A Mancuso","doi":"10.1016/j.trsl.2025.01.002","DOIUrl":"10.1016/j.trsl.2025.01.002","url":null,"abstract":"The organ-level molecular response to cardiac surgery with cardiopulmonary bypass (CPB) remains inadequately understood and may be heterogeneous. Here, we measured organ-specific gene expression in a piglet model of CPB with deep hypothermic circulatory arrest (DHCA). Infant piglets underwent peripheral CPB with 75 min of DHCA and 6 h of critical care after separation from CPB. Mechanically ventilated animals served as controls. Tissue was obtained from the lung, kidney, liver, heart, and ileum. RNA sequencing was performed using NovaSeq 6000 and evaluated via differentially expressed gene (DEG) and pathway/network analyses. CPB/DHCA induced significant transcriptomic alterations, with greater changes seen in liver (2,166 DEGs), heart (775 DEGs), and kidney (1,759 DEGs) compared to lung (401 DEGs) and ileum (11 DEGs), and little overlap across organs (<20 % differentially expressed in >1 organ). Key upregulated systems included ribosomal proliferation and mitochondrial assembly in the liver, oxidative stress response and proximal tubular repair in the kidney, myofilament structural genes and pro-hypertrophy pathways in the heart, and solute channels and arginine metabolism in the lung. Downregulation of adaptive immunity genes occurred in multiple organs. Transcriptomics could inform the investigation of targeted therapies and adverse event screening after cardiac surgery.","PeriodicalId":94257,"journal":{"name":"Translational research : the journal of laboratory and clinical medicine","volume":" ","pages":"64-74"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the impact of e-cigarettes on human barrier systems: A systematic review. 评估电子烟对人体屏障系统的影响：一项系统综述。

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-01-14 DOI: 10.1016/j.trsl.2025.01.001

Gabriella Lupo, Carmelina Daniela Anfuso, Giuseppe Smecca, Alessia Cosentino, Aleksandra Agafonova, Chiara Prinzi, Rosario Junior Ferrauto, Stefano Turzo, Venerando Rapisarda, Caterina Ledda

{"title":"Assessing the impact of e-cigarettes on human barrier systems: A systematic review.","authors":"Gabriella Lupo, Carmelina Daniela Anfuso, Giuseppe Smecca, Alessia Cosentino, Aleksandra Agafonova, Chiara Prinzi, Rosario Junior Ferrauto, Stefano Turzo, Venerando Rapisarda, Caterina Ledda","doi":"10.1016/j.trsl.2025.01.001","DOIUrl":"https://doi.org/10.1016/j.trsl.2025.01.001","url":null,"abstract":"The use of e-cigarettes has grown rapidly in recent years, raising concerns about their impact on human health, particularly on critical physiological barriers such as the blood-brain barrier (BBB), alveolar-capillary barrier, and vascular systems. This systematic review evaluates the current literature on the effects of e-cigarette exposure on these barrier systems. E-cigarettes, regardless of nicotine content, have been shown to induce oxidative stress, inflammation, and disruption of tight junction proteins, leading to impaired barrier function. Key findings include compromised pulmonary function, increased vascular stiffness, and neuroinflammation. The review highlights potential long-term health risks associated with e-cigarette use, such as cardiovascular disease, neurodevelopmental disorders, and multi-organ fibrosis, and emphasizes the need for public health interventions to regulate e-cigarette use, especially in vulnerable populations like pregnant women and adolescents.","PeriodicalId":94257,"journal":{"name":"Translational research : the journal of laboratory and clinical medicine","volume":"277 ","pages":"39-63"},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sodium-glucose co-transporter 2 (SGLT-2) inhibitors ameliorate renal ischemia-reperfusion injury (IRI) by modulating autophagic processes. 钠-葡萄糖共转运蛋白2 （SGLT-2）抑制剂通过调节自噬过程改善肾缺血再灌注损伤（IRI）。

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-01-04 DOI: 10.1016/j.trsl.2024.12.006

Mengmeng Liu, Yuanqing Yao, Fangyan Tan, Jing Wang, Rong Hu, Jianlin Du, Yonghong Jiang, Xin Yuan

{"title":"Sodium-glucose co-transporter 2 (SGLT-2) inhibitors ameliorate renal ischemia-reperfusion injury (IRI) by modulating autophagic processes.","authors":"Mengmeng Liu, Yuanqing Yao, Fangyan Tan, Jing Wang, Rong Hu, Jianlin Du, Yonghong Jiang, Xin Yuan","doi":"10.1016/j.trsl.2024.12.006","DOIUrl":"https://doi.org/10.1016/j.trsl.2024.12.006","url":null,"abstract":"Renal ischemia-reperfusion injury (IRI) is a common clinical condition that currently lacks effective treatment options. Inhibitors targeting the sodium-glucose co-transporter-2 (SGLT-2), recognized for their role in managing hyperglycemia, have demonstrated efficacy in enhancing the health outcomes for diabetic patients grappling with chronic kidney disease. Nevertheless, the precise impact of SGLT-2 inhibitors on renal ischemia-reperfusion injury (IRI) and the corresponding transcriptomic alterations remain to be elucidated. In our research, we developed a model of IRI using male C57BL/6 mice by clamping the unilateral renal artery and administering empagliflozin Transcriptomic alterations were analyzed using RNA sequencing (RNA-Seq), complemented by proteomic analysis to investigate the effects of empagliflozin. Histological assessments revealed increased renal inflammatory cell infiltration, widespread renal tubular injury, and elevated autophagosomes formation in the IRI group compared to controls. These pathological changes were significantly attenuated following empagliflozin treatment. Besides, renal function impairment can be alleviated in empagliflozin-treated group. RNA-Seq analysis identified lysosomal autophagy as a key biological process in IRI mice. Empagliflozin exerted a renoprotective effect by downregulating lysosome-associated membrane proteins, primarily LAMP1, LAMP2, and LAMP4 (CD68), through the PI3K-Akt, MAPK, and mTOR signaling pathways, thereby inhibiting autophagic processes. In conclusion, this study highlights enhanced inflammation and disrupted metabolism as hallmark transcriptomic signatures of renal. Furthermore, it demonstrates the renoprotective effects of empagliflozin in alleviating renal IRI by modulating autophagic processes.","PeriodicalId":94257,"journal":{"name":"Translational research : the journal of laboratory and clinical medicine","volume":"277 ","pages":"27-38"},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canagliflozin inhibits hedgehog interacting protein (Hhip) induction of tubulopathy in diabetic Akita mice. 卡格列净抑制刺猬相互作用蛋白（hip）诱导糖尿病秋田小鼠小管病变。

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-01-03 DOI: 10.1016/j.trsl.2024.12.005

Shiao-Ying Chang, Min-Chun Liao, Kana N Miyata, Yuchao Pang, Xin-Ping Zhao, Junzheng Peng, Alain Rivard, Julie R Ingelfinger, John S D Chan, Shao-Ling Zhang

{"title":"Canagliflozin inhibits hedgehog interacting protein (Hhip) induction of tubulopathy in diabetic Akita mice.","authors":"Shiao-Ying Chang, Min-Chun Liao, Kana N Miyata, Yuchao Pang, Xin-Ping Zhao, Junzheng Peng, Alain Rivard, Julie R Ingelfinger, John S D Chan, Shao-Ling Zhang","doi":"10.1016/j.trsl.2024.12.005","DOIUrl":"10.1016/j.trsl.2024.12.005","url":null,"abstract":"Renal hedgehog interacting protein (Hhip) activates sodium-glucose cotransporter 2 (Sglt2) expression and promotes tubular senescence in murine diabetic kidney disease (DKD), yet its underlying mechanism(s) are poorly understood. Here we study the effect of the SGLT2 inhibitor, canagliflozin on tubulopathy (fibrosis and apoptosis) in Akita/HhipRPTC-transgenic (Tg) mice with overexpression of Hhip in their renal proximal tubular cells (RPTCs) and its relevant mechanisms. The DKD-tubulopathy with pronounced Sglt2 expression was aggravated in the kidney of Akita/HhipRPTC-Tg cf. Akita/non-Tg mice. A strong association was observed between Hhip and tubular senescence in Nephroseq from the Nakagawa chronic kidney disease study. Both in vivo and in vitro, excessive Hhip in RPTCs triggered RPTC senescence (polyploidization and cytoskeleton destabilization) and released extracellular vesicles (EVs) carrying Hhip (EVsHhip), most of which were apoptotic bodies (ABsHhip) or microvesicles (MVsHhip) and little exosomes (EXOsHhip). Further, Hhip stimulated β2-microglobulin, which further interacts with EVsHhip, together facilitating RPTC turn-over from cellular senescence to fibrosis and/or apoptosis, ultimately leading to advanced tubulopathy. In contrast, canagliflozin administration offset the action of Hhip in RPTCs, thereby preventing DKD progression. In conclusion, canagliflozin prevented excessive Hhip-mediated tubulopathy, possibly via the inhibition of excessive Hhip carried by extracellular vehicles in DKD.","PeriodicalId":94257,"journal":{"name":"Translational research : the journal of laboratory and clinical medicine","volume":" ","pages":"13-26"},"PeriodicalIF":0.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Characterization of upregulated adhesion GPCRs in acute myeloid leukemia" [Transl Res. 2019 Oct:212:26-35. doi: 10.1016/j.trsl.2019.05.004. Epub 2019 May 17.]. “急性髓性白血病中粘附性gpcr上调的表征”的更正[翻译]. 2019年10月：22 -35。doi: 10.1016 / j.trsl.2019.05.004。[Epub 2019年5月17日]。

Translational research : the journal of laboratory and clinical medicine Pub Date : 2025-01-01 Epub Date: 2024-11-29 DOI: 10.1016/j.trsl.2024.11.003

Jiawen Yang, Sharon Wu, Houda Alachkar

引用次数: 0