Journal of biomedical research & environmental sciences最新文献

{"title":"Administration of HeberFERON in Patients with Persistent Oropharyngeal SARS-CoV-2 Wuhan/D614G Strain Viral Shedding","authors":"I. Campa-Legrá, J. Lence, Marel Alonso-Valdés, Marisol Diaz-Galvez, Adriana Sin-Mayor, M. García-Sánchez, Sara Martinez-Martin, J. Perez-Escribano, Idelsis Esquivel-Moynelo, Y. Duncan-Roberts, Claudia Martu00ednez-Suarez, Abraham Beato-Canfuk, I. Bello-Rivero","doi":"10.37871/jbres1380","DOIUrl":"https://doi.org/10.37871/jbres1380","url":null,"abstract":"Study background: HeberFERON accelerates SARS-CoV-2 clearance in COVID-19 cases. Considering this we evaluated the employment of HeberFERON in patients with more than 14 days of viral shedding. Methods: This is a case series study of mild or moderate ill patients with laboratory-confirmed SARS-CoV-2 from one hospital in Havana, Cuba. We evaluated the effect and safety of HeberFERON in patients previously treated with Heberon Apha R that resulted with prolonged viral shedding. All patients received lopinavir-ritonavir 200/50 mg every 12 h and chloroquine 250 mg every 12 h. The primary endpoint was the time to negativization of viral RNA in patients with persistent viral shedding. The protocol was approved by the Ethics Committee of the Luis Diaz Soto Hospital. Results: The characteristics of the individuals included the age ranged from 19-87 years with a mean of 40 years, (Study and Control I groups), while in the Control group II the mean age was 43.8 years. Leukocytes, platelets, neutrophils, and eosinophils, show a significantly lower counts in the groups with viral persistence. Under IFN treatment the median viral shedding duration from diagnosis were 21 days and 19 days in Study group and Control group II, respectively. The Control group I showed a median viral shedding of 11 days (log-rank p = 0.000). Significant longer median viral negativization time (19 days) of symptomatic than asymptomatic patients (11 days, Long-rank p = 0.004), was observed. In patients under Heberon Alpha R treatment that resulted persistent for viral presence, the median time to viral negativization was 7 days for the period of administration of HeberFERON. Being symptomatic at diagnosis was significantly associated with viral persistence. The HeberFERON showed an adequate safety profile. Conclusion: HeberFERON showed a safe and rapid negativization of patients with viral persistence, achieving negativization in more than 50% of patients in 7 days.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84979893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical and Analytical Implications of GATES/GEB Principles","authors":"A. Michałowska-Kaczmarczyk, T. Michałowski","doi":"10.37871/jbres1373","DOIUrl":"https://doi.org/10.37871/jbres1373","url":null,"abstract":"The fundamental property of electrolytic systems involved with linear combination f12 = 2∙f(O) – f(H) of elemental balances: f1 = f(H) for Y1 = H, and f2 = f(O) for Y2 = O, is presented. The dependency/independency of the f12 on Charge Balance (f0 = ChB) and other elemental and/or core balances fk = f(Yk) (k = 3,…,K) is the general criterion distinguishing between non-redox and redox systems. The f12 related to a redox system is the primary form of a Generalized Electron Balance (GEB), formulated for redox systems within the Generalized Approach to Electrolytic System (GATES) as GATES/GEB ⊂ GATES. The set of K balances f0,f12,f3,…,fK is necessary/ sufficient/needed to solve an electrolytic redox system, while the K-1 balances f0,f3,…,fK are the set applied to solve an electrolytic non-redox system. The identity (0 = 0) procedure of checking the linear independency/ dependency property of f12 within the set f0,f12,f3,…,fK (i) provides the criterion distinguishing between the redox and non-redox systems and (ii) specifies Oxidation Numbers (ONs) of elements in particular components of the system, and in the species formed in the system. Some chemical concepts, such as oxidant, reductant, oxidation number, equivalent mass, stoichiometry, perceived as derivative within GATES, are indicated. All the information is gained on the basis of the titration Ce(SO4)2 (C) + H2SO4 (C1) + CO2 (C2) ⇨ FeSO4 (C0) + H2SO4 (C01) + CO2 (C02), simulated with use of the iterative computer program MATLAB.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81920028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Developments and Applications of Single-Cell RNA Sequencing Technology in Cell Classification","authors":"Safir Ullah Khan, Munir Ullah Khan","doi":"10.37871/jbres1383","DOIUrl":"https://doi.org/10.37871/jbres1383","url":null,"abstract":"Multicellular organisms have many cell types and are complex, and heterogeneity is common among cells. Single-Cell RNA Sequencing (scRNA-SEQ) is a new technique for studying the transcriptional activity of a single cell that is still in its early stages of development. It generates transcriptional profiles from thousands of parallel cells to reveal the differential expression of individual cell genomes. They reflect the heterogeneity between cells to identify different cell types and form cell maps of tissues or organs, which play an essential role in biology and clinical medicine. Based on the introduction and comparison of the scRNA-SEQ sequencing platform, this paper focuses on the application of scRNA-SEQ in the exploration of cell types in the nervous system and immune system and summarizes the research results of the combination of scRNA-SEQ and spatial transcriptome technology.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77830334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avifauna within the Main Campus of Imam Mohammad Ibn Saud Islamic University Riyadh, Saudi Arabia","authors":"H. A. Rudayni","doi":"10.37871/jbres1379","DOIUrl":"https://doi.org/10.37871/jbres1379","url":null,"abstract":"Avian diversity of two zones within the main campus of IMSIU University was studied during the period from July 2019 to June 2020. A total number of 1,653 individual birds was recorded across the main campus which falls under fifteen species belonging to thirteen families. Out of this number, 760 individual of them were recorded in Zone-A versus 893 individual in Zone-B. Species richness in Zone-A was 15 while species richness in Zone-B was 13. Census index of the Birds was recorded as follows: 4.6 Birds/ha and 7.9 Birds/ha in both Zones A and B, respectively. Bird species sighted was higher in Zone-B as compared with Zone-A, as indicated by Shannon-Wiener Diversity Index (H) which accounts for 1.83 in Zone-A and 1.65 in Zone-B. Pigeon (Columba livia), House Sparrow (Passer domesticus) and Laughing Dove (Spilopelia senegalensis) were among the most frequently sighted. From the current study, it is recommended that Zone-B be pedestrianized for the enrichment and protection of the avian species. Also, new policies should be put forward by the relevant authority within the main campus to maintain avian diversity, such as expanding afforestation and green landscapes, in addition to the establishment of artificial lakes and ponds.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84607237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stemphylium and Ulocladium between Benefit and Harmful","authors":"W. Elkhateeb, G. Daba","doi":"10.37871/jbres1356","DOIUrl":"https://doi.org/10.37871/jbres1356","url":null,"abstract":"Background: Emerging of microbial resistance, spread of life-threatening diseases, and biological control of pathogens destroying economically important crops, are serious problems that encourage scientists to search for unusual sources for novel compounds with biological activities. Fungi are promising sources for such compounds due to their ability to produce variety of secondary metabolites that could be, if truly investigated, the solution for currently serious problems. Aim: The aim of this review is to highlight the diversity of compounds produced by endophytic Stemphylium and Ulocladium and represents their ability to produce biologically diverse metabolites. Materials and methods: This was a narrative review. A comprehensive literature search was done using PubMed, Google Scholar, Scopus, and EMBASE using the keywords, Stemphylium; Ulocladium; Secondary metabolites; biological activities. Results: Many studies reported that the endophytic Ulocladium especially, Ulocladium atrum Preuss, showed promising biocontrol activity against Botrytis cinerea on crops cultivated in the greenhouse and the field. The endophytic fungus Stemphylium especially, Stemphylium globuliferum was isolated from stem tissues of the Moroccan medicinal plant Mentha pulegium. Extracts of the fungus exhibited significant cytotoxicity when tested in vitro against L5178Y cells. Conclusion: Endophytic fungi are a noble and consistent source of unique natural mixtures with a high level of biodiversity and may also yield several compounds of pharmaceutical significance, which is currently attracting scientific surveys worldwide. Every study conducted on Stemphylium and Ulocladium resulted in discovery of new metabolites or pointing to a possible application, which made Stemphylium and Ulocladium species potential source of pharmaceuticals and attracted attention for further investigations of their biological control.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82203952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Cytomegalovirus, Rubella and Toxoplasma gondii among Internally Displaced Women of District North Waziristan, Pakistan with Home Obstetric History","authors":"Saddam S, Ghadir Ali, Abdul Rehman, A. Shahzad","doi":"10.37871/jbres1377","DOIUrl":"https://doi.org/10.37871/jbres1377","url":null,"abstract":"The present study was conducted to estimate the prevalence rate and to identify risk factors of Cytomegalovirus, Rubella and Toxoplasma gondii among internally displaced women of District North Waziristan, Pakistan with home obstetric history. A total of 387 blood samples were collected from internally displaced infected women with the help of a comprehensive filled data form. To determine levels of Cytomegalovirus, Rubella and Toxoplasma gondii Infection, all blood samples were qualitative and quantitative analyzed using Immune chromatographic and Chemiluminescence Microparticle Immunoassay (CMIA) technique. Among 387 blood samples, the total numbers of reactive samples observed were 341 (88.11%) and non-reactive samples were 46 (11.88%). Of 341 (88.11%) reactive samples, the highest ratio 168 (49.26%) was reported for the co-infection of Cytomegalovirus and Rubella, followed by 76 (22.28%) for Cytomegalovirus, Rubella and Toxoplasma gondii combined. In reactive samples, the highest ratio of IgG was noted in order of CMV 22 (64.70%), Rubella 21 (67.74%) and Toxoplasma gondii 16 (72.72%), followed by IgG+IgM and IgM only. Regarding age-wise distribution, the highest ratio 144 (90.56%) of total reactive samples of Cytomegalovirus, Rubella and Toxoplasma gondii was reported in the 25-32 years old group of individuals. Similarly, the highest ratio 53 (48.18%) and 60 (41.66%) of co-infection among reactive samples for Cytomegalovirus, and Rubella was observed in 18-24 and 25-32 years old group. Further, the highest ratio of 100 (40.32%) of risk factors was observed in infected patients who were exposed to a poor hygienic environment and 57 (44.18%) of infected patients had complications of Severe UTI followed by others. Hence, it was revealed that Cytomegalovirus, Rubella and Toxoplasma gondii infection was most prevalent in internally displaced women of District North Waziristan, Pakistan. Therefore, an urgent base prophylactic approaches should need to be addressed to reduce the risk of the spread of disease in this locality.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91263558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Adverse Events Post-COVID-19 Vaccination amongst the Adult Zambian Population","authors":"M. Chileshe, Geoffrey Mupeta, M. Kasanga, Ruth Lindizyani Mfune, S. Mudenda, Mildred Biemba, Nzooma M Shimaponda-Mataa, M. Sinkala, V. Daka","doi":"10.37871/jbres1389","DOIUrl":"https://doi.org/10.37871/jbres1389","url":null,"abstract":"Introduction: COVID-19 continues to be a public health concern despite several practical interventions to curb its spread. Now, widespread vaccination appears to be the most important strategy in winning the fight against this deadly pandemic by promoting herd immunity. However, a lack of independent and local studies on vaccines’ safety may adversely impact the vaccine uptake and eventually the overarching goal of reducing disease transmission. Objective: To determine the prevalence of adverse events post COVID-19 vaccination amongst the adult Zambia population. Methods: This was a descriptive cross-sectional study conducted among recipients of COVID-19 vaccines in Zambia. The study was conducted between July and August 2021. 584 adult individuals who had access to the questionnaire were enrolled. Relationships between patients' demographics and Adverse Events (AEs) were assessed using the chi-square (χ2) test given the categorical nature of the data. Results: Pain at the injection site (79.8%), headache (57.4%), fatigue (55.5%), chills (52.6%), fever (42.3%), and joint pains (37.5%) were the most commonly reported adverse events. When stratified by gender, pain at the injection site (p = 0.01), limitation of arm movement (p < 0.001), chills (p = 0.017), headache (p = 0.044), fatigue (p = 0.004), nausea or vomiting (p < 0.001) were statistically significant, with females mostly affected. There was a statistically significant difference in the case of limitation of arm movement (20.7% vs. 9.9%, p = 0.031), chills (54.8% vs. 36.6%, p = 0.004), headache (59.6% vs. 40.8%, p = 0.003) and fatigue (57.5% vs. 40.8%, p = 0.008) between those 49 years and below compared to those above 50 years. Conclusion: Our study provides evidence of adverse events being experienced by the recipient of COVID-19 vaccines and that these have been mainly mild. Healthcare authorities need to educate the public about the possible adverse events associated with COVID-19 vaccines and how to report these events should they experience some. This will improve pharmacovigilance of adverse events associated with COVID-19 vaccines.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84780920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Application of Genetically Engineered Ribosome-Inactivating Toxin Proteins for Cancer","authors":"S. Khan, M. U. Khan","doi":"10.37871/jbres1375","DOIUrl":"https://doi.org/10.37871/jbres1375","url":null,"abstract":"Recently, Ribosome-Inactivating Proteins (RIPs) as a class of anticancer medicines have garnered considerable attention due to their novel anticancer mechanism. Although the medications are small, RIPs utilize the Large-Size Effect (LSE) to block the efflux procedure that are regulated through Drug Resistance Transporters (DRTs), and protect host cells from drug transfection. There are many significant challenges for their therapeutic applications that seriously restrict their usefulness, particularly their strategy towards tumor cells. The primary objective of this review is to emphasize Trichosanthin (TCS) along with Gelonin (Gel) and additional types of RIPs, particularly scorpion venom-derived RIPs, to demonstrate that they should be grappling through what kinds of bio-barriers to overcome in cancer therapeutic science. Next, we will emphasize the latest state-of-the-art in providing cancer treatment RIPs.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81498232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Torsion Gravity","authors":"K. Si","doi":"10.37871/jbres1388","DOIUrl":"https://doi.org/10.37871/jbres1388","url":null,"abstract":"Based on the discovery by astrophysicists of dark matter halos around galaxies, stars and planets, it became possible to abandon the speculative concept of the spatial curvature of Einstein's space-time fabric and geometric gravity. Torsional gravity and spinors in fundamental theoretical physics should be based on a new cosmology, including a dark matter halo rotating with planets, stars and galaxies and forming funnels in the continuous space environment of a quantum vacuum (dark matter). The article discusses the nature of tornado and tropical hurricanes.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"276 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73482580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal-Based Nanomaterials Incorporate with Ultrasound as Acceptable Approach towards Cancer Therapy","authors":"Xiaoxiao He, Shiyue Chen, Xiang Mao","doi":"10.37871/jbres1354","DOIUrl":"https://doi.org/10.37871/jbres1354","url":null,"abstract":"Among current biological researches, there have a plenty of works related cancer therapy issues by using functional or pure-phased composites in non-invasive strategies. Especially in fabricating anticancer candidates, functional composites are divided into different sorts with different characteristics. Additionally, nanotechnology provides various approaches in utilizing composites’ functionality for cancer diagnostics and therapeutics. Compared with previous Photodynamic Therapy (PDT), Photo-Thermal Therapy (PTT), chemotherapy and radiotherapy, ultrasound is used to activate sonosensitizer to produce cytotoxic Reactive Oxygen Species (ROS) toward target cancer cells. In recent years, the form of Sonodynamic Therapy (SDT) has been making much effort to develop highly efficient metal based Nanomaterials (NMs) as sonosensitizers, which can efficiently generate ROS and has the advantages of deeper tissue penetration. However, the traditional sonosensitizers, such as porphyrins, hypericin, and curcumins suffer from complex synthesis, poor water solubility, and low tumor targeting efficacy. For contrasting this limitation, the metal based inorganic NMs show biocompatibility, controllable physicochemical properties, and ease of achieving multifunctional properties, which greatly expanded their application in SDT. In this review, we systematically summarize the metal based inorganic NMs as carrier of molecular sonosensitizers, and produce ROS under ultrasound. Moreover, the prospects of advanced metal based further materials application are also discussed.","PeriodicalId":94067,"journal":{"name":"Journal of biomedical research & environmental sciences","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86819409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}