Comparative medicine最新文献_第5页

Erratum: Unique Presentations of Burkholderia gladioli Infections in Several Strains of Immunocompromised Mice. 勘误：几种免疫力低下小鼠感染格氏伯克霍尔德氏菌的独特表现。

Comparative medicine Pub Date : 2024-02-01 Epub Date: 2024-03-04 DOI: 10.30802/AALAS-CM-24-000012

引用次数: 0

Consistency in Reporting of Loss of Righting Reflex for Assessment of General Anesthesia in Rats and Mice: A Systematic Review. 用于评估大鼠和小鼠全身麻醉的右反射丧失报告的一致性：系统性综述。

Comparative medicine Pub Date : 2024-02-01 DOI: 10.30802/AALAS-CM-23-000063

Michael Z Teng, Dexter Merenick, Anisha Jessel, Heather Ganshorn, Daniel S J Pang

{"title":"Consistency in Reporting of Loss of Righting Reflex for Assessment of General Anesthesia in Rats and Mice: A Systematic Review.","authors":"Michael Z Teng, Dexter Merenick, Anisha Jessel, Heather Ganshorn, Daniel S J Pang","doi":"10.30802/AALAS-CM-23-000063","DOIUrl":"10.30802/AALAS-CM-23-000063","url":null,"abstract":"General anesthesia induces a reversible loss of consciousness (LOC), a state that is characterized by the inability to feel pain. Identifying LOC in animals poses unique challenges, because the method most commonly used in humans, responding to questions, cannot be used in animals. For over a century, loss of righting reflex (LORR) has been used to assess LOC in animals. This is the only animal method that correlates directly with LOC in humans and has become the standard proxy measure used in research. However, the reporting of how LORR is assessed varies extensively. This systematic literature review examined the consistency and completeness of LORR methods used in rats and mice. The terms 'righting reflex,' 'anesthesia,' 'conscious,' 'rats,' 'mice,' and their derivatives were used to search 5 electronic databases. The abstracts of the 985 articles identified were screened for indications that the study assessed LORR in mice or rats. Full texts of selected articles were reviewed for LORR methodological completeness, with reported methods categorized by 1) animal placement method, 2) behavioral presence of righting reflex, 3) duration of LORR testing, 4) behavioral LORR, and 5) animal position for testing LORR. Only 22 papers reported on all 5 methodological categories. Of the 22 papers, 21 used unique LORR methodologies, with descriptions of LORR methods differing in at least one category as compared with all other studies. This variability indicates that even papers that included all 5 categories still had substantial differences in their methodological descriptions. These findings reveal substantial inconsistencies in LORR methodology and reporting in the biomedical literature likely compromising study replicability and data interpretation.","PeriodicalId":93950,"journal":{"name":"Comparative medicine","volume":"74 1","pages":"12-18"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10938561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Pharmacokinetics of a Single Transdermal Dose of Mirtazapine in Rhesus Macaques (Macaca mulatta). 更正：米氮平在猕猴体内单次透皮给药的药代动力学。

Comparative medicine Pub Date : 2024-02-01 DOI: 10.30802/AALAS-CM-24-000010

引用次数: 0

Erratum: AC-DC Electropenetrography as a Tool to Quantify Probing and Ingestion Behaviors of the Yellow Fever Mosquito (Aedes aegypti) on Mice in Biocontainment. 勘误：将 AC-DC 电穿孔成像技术作为量化黄热病蚊子（埃及伊蚊）对生物隔离小鼠的探查和摄食行为的工具。

Comparative medicine Pub Date : 2024-02-01 Epub Date: 2024-03-04 DOI: 10.30802/AALAS-CM-24-000009

Lyndsi D Vaughan, Samuel B Jameson, Dawn M Wesson, Kristopher S Silver, Dana N Mitzel, Georgina L Dobek, Berlin Londoño-Renteria

引用次数: 0

High-pressure Hydrodynamic Injection as a Method of Establishing Hepatitis B Virus Infection in Mice. 高压水动力注射作为一种确定小鼠乙型肝炎病毒感染的方法

Comparative medicine Pub Date : 2024-02-01 Epub Date: 2024-02-16 DOI: 10.30802/AALAS-CM-23-000050

Juan Wen, Guoli Chen, Tianshun Wang, Wan Yu, Zhengyun Liu, Huan Wang

引用次数: 0

AC-DC Electropenetrography as a Tool to Quantify Probing and Ingestion Behaviors of the Yellow Fever Mosquito (Aedes aegypti) on Mice in Biocontainment. 将 AC-DC 电笔描记术作为一种工具，量化小鼠在生物隔离饲养条件下的蚊子（埃及伊蚊）探查和注射行为。

Comparative medicine Pub Date : 2023-12-01 Epub Date: 2023-12-12 DOI: 10.30802/AALAS-CM-23-000037

{"title":"AC-DC Electropenetrography as a Tool to Quantify Probing and Ingestion Behaviors of the Yellow Fever Mosquito (Aedes aegypti) on Mice in Biocontainment.","authors":"","doi":"10.30802/AALAS-CM-23-000037","DOIUrl":"10.30802/AALAS-CM-23-000037","url":null,"abstract":"A detailed understanding of mosquito probing and ingestion behaviors is crucial in developing novel interventions to interrupt the transmission of important human and veterinary pathogens, but these behaviors are difficult to observe as the mouthparts are inserted into the skin of the host. Electropenetrography (EPG) allows indirect observation, recording, and quantification of probing and ingestion behaviors of arthropods by visualizing the electrical waveform associated with these behaviors. The study of mosquito probing and ingestion behaviors has been limited to the use of human hands as host, which is not suitable for pathogen transmission studies. Mouse models of mosquito-borne diseases are a widely used and indispensable tool in this research, but previous attempts to use direct current EPG to study mosquito probing behaviors on mice have been unsuccessful. Accordingly, the present study used alternating or direct current (AC-DC) EPG to observe the ingestion behaviors of adult Aedes aegypti mosquitoes on a mouse host in real time under BSL-2 containment conditions with enhanced BSL-3 practices. Our results show that waveform families previously identified during Ae. aegypti probing and ingestion on human hands were observed using 100 mV of AC at an input resistance (Ri) of 107 Ohms (Ω) on CD1 mice. This work is a proof of concept for using mouse models for studying mosquito probing and ingestion behaviors with AC-DC EPG. In addition, these data show that the experimental setup used in these experiments is sufficient for conducting studies on mosquito probing and ingestion behaviors under BSL-2 containment conditions enhanced with BSL-3 practices. This work will serve as a foundation for using EPG to investigate the effects of pathogen infection on mosquito probing behaviors and to understand the real-time dynamics of pathogen transmission.","PeriodicalId":93950,"journal":{"name":"Comparative medicine","volume":" ","pages":"25"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10752365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Exogenous Erythropoietin on Rabbit (Oryctolagus cuniculus) Hematological and Biochemical Parameters. 外源性促红细胞生成素对兔血液学和生化指标的影响

Comparative medicine Pub Date : 2023-12-01 Epub Date: 2023-11-15 DOI: 10.30802/AALAS-CM-22-000107

Jessica K Levine, Josilene Nascimento Seixas, Jana M Ritter, Amanda Y Liew, Cassandra M Tansey

引用次数: 0

Antibiotic Treatment of Corynebacterium bovis-associated Clinical Disease in NSG Mice. 抗生素治疗NSG小鼠与牛棒状杆菌相关的临床疾病

Comparative medicine Pub Date : 2023-12-01 Epub Date: 2023-12-16 DOI: 10.30802/AALAS-CM-23-000039

Elena M Pires, Umarani Pugazhenthi, Michael K Fink, Lauren M Habenicht, Derek L Fong, Jori K Leszczynski, Michael J Schurr, Christopher A Manuel

{"title":"Antibiotic Treatment of Corynebacterium bovis-associated Clinical Disease in NSG Mice.","authors":"Elena M Pires, Umarani Pugazhenthi, Michael K Fink, Lauren M Habenicht, Derek L Fong, Jori K Leszczynski, Michael J Schurr, Christopher A Manuel","doi":"10.30802/AALAS-CM-23-000039","DOIUrl":"10.30802/AALAS-CM-23-000039","url":null,"abstract":"Depending on the strain of immunodeficient mice, Corynebacterium bovis infection can be asymptomatic or cause transient or prolonged skin disease. C. bovis infection of NOD. Cg- Prkdcscid Il2rgtm1Wjl /SzJ (NSG) mice results in clinical skin disease that progresses in severity. Amoxicillin metaphylaxic and prophylaxic therapy prevents transmission and infection of mice after exposure to C. bovis and inhibits the growth of C. bovis isolates at therapeutic doses that are clinically achievable in mice. Amoxicillin is not efficacious for treatment of transient clinical skin disease in athymic nude mice, but the efficacy of amoxicillin treatment has not previously been characterized in C. bovis -infected NSG mice. In the current study, NSG mice were treated with amoxicillin beginning at 5 wk after exposure to C. bovis, at which time they had well-established clinical signs of disease. Clinical signs were scored to assess disease progression, regression, and reappearance. Our results showed that amoxicillin treatment for 3 or 6 wk reduced the clinical scores of NSG mice with C. bovis -associated clinical disease. In addition, withdrawal of treatment led to the recurrence of clinical signs. Collectively, our data suggest that amoxicillin treatment is effective in alleviating the clinical signs associated with C. bovis infection for the duration of treatment in NSG mice. Clinical intervention with antibiotics for C. bovis -infected NSG mice can be an option for management of C. bovis -related clinical disease either before or during facility-wide remediation efforts.","PeriodicalId":93950,"journal":{"name":"Comparative medicine","volume":" ","pages":"461-465"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10752358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of the Effects of Some Extrinsic Factors on Mice Used in Research. 综述某些外在因素对研究用小鼠的影响

Comparative medicine Pub Date : 2023-12-01 DOI: 10.30802/AALAS-CM-23-000028

Alfonso S Gozalo, William R Elkins

{"title":"A Review of the Effects of Some Extrinsic Factors on Mice Used in Research.","authors":"Alfonso S Gozalo, William R Elkins","doi":"10.30802/AALAS-CM-23-000028","DOIUrl":"10.30802/AALAS-CM-23-000028","url":null,"abstract":"Animals have been used in research for over 2,000 y. From very crude experiments conducted by ancient scholars, animal research, as a science, was refined over hundreds of years to what we know it as today. However, the housing conditions of animals used for research did not improve significantly until less than 100 years ago when guidelines for housing research animals were first published. In addition, it was not until relatively recently that some extrinsic factors were recognized as a research variable, even when animals were housed under recommended guidelines. For example, temperature, humidity, light, noise, vibration, diet, water, caging, bedding, etc., can all potentially affect research using mice, contributing the inability of others to reproduce published findings. Consequently, these external factors should be carefully considered in the design, planning, and execution of animal experiments. In addition, as recommended by others, the housing and husbandry conditions of the animals should be described in detail in publications resulting from animal research to improve study reproducibility. Here, we briefly review some common, and less common, external factors that affect research in one of the most popular animal models, the mouse.","PeriodicalId":93950,"journal":{"name":"Comparative medicine","volume":"73 6","pages":"413-431"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10752364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139432950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics of a Single Transdermal Dose of Mirtazapine in Rhesus Macaques (Macaca mulatta). 猕猴单次透皮服用米氮平的药代动力学

Comparative medicine Pub Date : 2023-12-01 DOI: 10.30802/AALAS-CM-23-000060

David W Bissinger, Luke A Wittenburg, Laura M Garzel, Diane E Stockinger, Gregory B Timmel

{"title":"Pharmacokinetics of a Single Transdermal Dose of Mirtazapine in Rhesus Macaques (Macaca mulatta).","authors":"David W Bissinger, Luke A Wittenburg, Laura M Garzel, Diane E Stockinger, Gregory B Timmel","doi":"10.30802/AALAS-CM-23-000060","DOIUrl":"10.30802/AALAS-CM-23-000060","url":null,"abstract":"Decreased appetite is a common clinical problem in captive rhesus macaques (Macaca mulatta). Mirtazapine, a tetracyclic antidepressant originally developed for humans, has shown promise as a safe and effective promoter of weight gain and appetite in several veterinary species including rhesus and cynomolgus macaques. Although mirtazapine is available as oral formulations, transdermal delivery in macaques with reduced appetite would allow quick, painless, topical application. Here we describe the pharmacokinetics of a single application of a widely available veterinary transdermal mirtazapine formulation in 6 rhesus macaques. A dose of 0.5 mg/kg of transdermal mirtazapine ointment that has proven to be effective in rhesus was applied to the caudal pinnae of 3 female and 3 male young adult macaques. Serum was collected at 0, 0.5, 1, 3, 6, 8, 12, 24, 36, 48, and 72 h after administration. Our data indicate transdermal mirtazapine is absorbed at a lower level in rhesus as compared with published values in domestic cats (rhesus peak serum concentration: 1.2 ± 0.3 ng/mL), while drug half-life is longer than that reported in cats (rhesus: 33 ± 7 h). Mirtazapine reaches peak plasma concentrations in rhesus at 16 ± 10 h after administration; our model indicates that up to 5 d of serial dosing may be necessary to reach steady state. Our preliminary data also suggest that sex differences may contribute to efficacy and/or indicate sex-based differences, as male macaques reached Tmax more quickly than females (19 ± 2 h in females and 8 ± 3 h in males) and showed higher variation in half-life (33 ± 4 h in females and 34 ± 11 h in males). While previous work indicates clinical efficacy of the 0.5-mg/kg dosage in macaques, further investigation is warranted to determine if rhesus may benefit from higher recommended doses than companion animal species.","PeriodicalId":93950,"journal":{"name":"Comparative medicine","volume":"73 6","pages":"432-438"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10752366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139432951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0